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Characteristics and biological control functions of Bacillus sp. PM8313 as a host-associated probiotic in red sea bream (Pagrus major) aquaculture
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Won Je Janga, Kyung-Bon Leeb, Mi-Hyeon Jeonc, Su-Jeong Leec, Sang Woo Hurd, Seunghan Leed, Bong-Joo Leed, e, Jong Min Leea, Kang-Woong Kimd, *, Eun-Woo Leec, *
Animal Nutrition | 2023, 12(1) : 20 - 31
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Animal Nutrition | 2023, 12(1): 20-31
• Original Research Article •
Characteristics and biological control functions of Bacillus sp. PM8313 as a host-associated probiotic in red sea bream (Pagrus major) aquaculture
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Won Je Janga, Kyung-Bon Leeb, Mi-Hyeon Jeonc, Su-Jeong Leec, Sang Woo Hurd, Seunghan Leed, Bong-Joo Leed, e, Jong Min Leea, Kang-Woong Kimd, *, Eun-Woo Leec, *
Affiliations
  • aDepartment of Biotechnology, Pukyong National University, Busan, 48513, South Korea
  • bDepartment of Biology Education College of Education, Chonnam National University, Gwangju, 61186, South Korea
  • cBiopharmaceutical Engineering Major, Division of Applied Bioengineering, Dong-Eui University, Busan, 47340, South Korea
  • dAquafeed Research Center, National Institute of Fisheries Science, Pohang, 37517, South Korea
  • eDepartment of Smart Fisheries Resources, College of Industrial Sciences, Kongju National University, Yesan, 32439, South Korea
Published: 2023-03-10 doi: 10.1016/j.aninu.2022.08.011
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Host-associated probiotics (HAPs) are bacteria originally isolated from rearing water or the host's gastrointestinal tract in order to enhance the host's growth and health. This study investigated the HAP potential of Bacillus sp. PM8313, isolated from wild red sea bream (Pagrus major), through characterization and feeding trials. Results based on in vitro tests showed that PM8313 is safe, confirming its hemolytic, cytotoxic, and antibiotic resistance. In addition, PM8313 showed advantages as a probiotic with high viability in the gastrointestinal model and a high cell adhesion rate. Whole-genome sequencing demonstrated that PM8313 has a 4,615,871 bp single circular chromosome and a guanine-cytosine content of 45.25%. It also showed the absence of genes encoding virulence factors, such as cytotoxin, enterotoxin, hemolysin, sphingomyelinase, and phospholipase. In the feeding trial, a supplemental diet of 1 × 108 CFU/g PM8313 positively altered the weight gain, digestive enzyme activity, and intestinal microbiota composition of red sea bream. Analysis of nonspecific immune parameters and immune-related gene expression, and a challenge test showed that PM8313 supplementation increases immunity and pathogenic bacteria resistance. Our findings suggest that PM8313 should be considered for application as a novel HAP to red sea bream aquaculture.

Host-associated probiotics  /  Immunity  /  Microbiota  /  Probiotics  /  Red sea bream
Won Je Jang, Kyung-Bon Lee, Mi-Hyeon Jeon, Su-Jeong Lee, Sang Woo Hur, Seunghan Lee, Bong-Joo Lee, Jong Min Lee, Kang-Woong Kim, Eun-Woo Lee. Characteristics and biological control functions of Bacillus sp. PM8313 as a host-associated probiotic in red sea bream (Pagrus major) aquaculture[J]. Animal Nutrition, 2023 , 12 (1) : 20 -31 . DOI: 10.1016/j.aninu.2022.08.011
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Year 2023 volume 12 Issue 1
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Article Info
doi: 10.1016/j.aninu.2022.08.011
  • Receive Date：2022-03-27
  • Online Date：2026-02-03
  • Published：2023-03-10
Article Data
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History
  • Received：2022-03-27
  • Revised：2022-07-29
  • Accepted：2022-08-10
Affiliations
    aDepartment of Biotechnology, Pukyong National University, Busan, 48513, South Korea
    bDepartment of Biology Education College of Education, Chonnam National University, Gwangju, 61186, South Korea
    cBiopharmaceutical Engineering Major, Division of Applied Bioengineering, Dong-Eui University, Busan, 47340, South Korea
    dAquafeed Research Center, National Institute of Fisheries Science, Pohang, 37517, South Korea
    eDepartment of Smart Fisheries Resources, College of Industrial Sciences, Kongju National University, Yesan, 32439, South Korea

Corresponding:

* Corresponding authors. E-mail addresses: (K.-W. Kim)
(E.-W. Lee).
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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