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Effect of Grifola frondosa polysaccharide on immune function and gut microbiota in mice
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Linlin Ma1, Xiaoliang Lin2, Ming Liang2, Jieyi Long2, Xian Qu1, Yi Yu2, Yifa Zhou1, Hairong Cheng1, *
Acupuncture and Herbal Medicine | 2025, 5(1) : 68 - 75
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Acupuncture and Herbal Medicine | 2025, 5(1): 68-75
• Original Articles •
Effect of Grifola frondosa polysaccharide on immune function and gut microbiota in mice
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Linlin Ma1, Xiaoliang Lin2, Ming Liang2, Jieyi Long2, Xian Qu1, Yi Yu2, Yifa Zhou1, Hairong Cheng1, *
Affiliations
  • 1Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory of Chemistry and Biology of Changbai Mountain NaturalDrugs, Northeast Normal University, Changchun, China
  • 2Infinitus China Ltd., Guangzhou, China.
About Author:

Linlin Ma and Xiaoliang Lin contributed equally to this work and should be considered as co-first authors.

Published: 2025-03-25 doi: 10.1097/HM9.0000000000000150
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Objective:

Grifola frondosa, a medicinal mushroom, is widely used to enhance immunity and treat cancer. Polysaccharides are its primary active components. We aimed to investigate the effects of the alkaloid G. frondosa polysaccharide (GFP) extract on immunity and gut microbiota.

Methods:

Alkaloid GFP was extracted using an alkaline extraction method, followed by hollow-fiber microfiltration. The molecular weight of alkaloid GFP was determined by high-performance gel permeation chromatography (HPGPC). Monosaccharide composition was analyzed by pre-column derivatization combined with high-performance liquid chromatography (HPLC). Methylation analysis was performed to characterize glycosidic linkages in alkaloid GFP. The immune function of alkaloid GFP was assessed in a cyclophosphamide (CTX)-induced immunosuppressive mouse model. Splenic lymphocyte proliferation, macrophage phagocytic capacity, and natural killer (NK) cell cytotoxicity were evaluated. The effect of alkaloid GFP on gut microbiota was assessed by 16S rRNA sequencing.

Results:

The molecular weight distribution of alkaloid GFP ranged from 17 to 18 kDa. The alkaloid GFP contained a β-(1→6)-glucan backbone branched at O-3 by β-1,3-D-Glcp. Oral administration of alkaloid GFP mitigated the effects of CTX on spleen index, splenic lymphocyte proliferation, and peritoneal macrophage phagocytosis. Additionally, alkaloid GFP improved the gut microbiota composition of immunosuppressed mice, increasing the relative abundances of Ligilactobacillus and Lactobacillus.

Conclusions:

Alkaloid GFP demonstrated immune-enhancing effects and gut microbiota regulatory activity, providing a basis for developing related health food ingredients.

Grifola frondosa  /  Gut microbiota  /  Immune activity  /  Polysaccharide
Linlin Ma, Xiaoliang Lin, Ming Liang, Jieyi Long, Xian Qu, Yi Yu, Yifa Zhou, Hairong Cheng. Effect of Grifola frondosa polysaccharide on immune function and gut microbiota in mice[J]. Acupuncture and Herbal Medicine, 2025 , 5 (1) : 68 -75 . DOI: 10.1097/HM9.0000000000000150
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Funding
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  • Infinitus Co., Ltd(2019009)
  • Scientific and Technologic Foundation of Jilin Province(20230202050NC)
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Year 2025 volume 5 Issue 1
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Article Info
doi: 10.1097/HM9.0000000000000150
  • Receive Date：2024-01-15
  • Online Date：2026-11-04
  • Published：2025-03-25
Article Data
Affiliations
History
  • Received：2024-01-15
  • Accepted：2025-02-18
Funding
Infinitus Co., Ltd(2019009)
Scientific and Technologic Foundation of Jilin Province(20230202050NC)
Affiliations
    1Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory of Chemistry and Biology of Changbai Mountain NaturalDrugs, Northeast Normal University, Changchun, China
    2Infinitus China Ltd., Guangzhou, China.

Corresponding:

* Hairong Cheng, E-mail:.
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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