中国新药杂志

高内涵技术在药物线粒体损伤毒性机制研究中的应用

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宋佳敏 ¹ , 王萌 ¹ , 陈美玲 ²

中国新药杂志 | 综述 2021,30(20): 1861-1866

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中国新药杂志 |综述 2021 , 30 (20) : 1861 -1866

高内涵技术在药物线粒体损伤毒性机制研究中的应用

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宋佳敏¹, 王萌¹, 陈美玲²

作者信息

¹天津中医药大学　组分中药国家重点实验室，天津　301617

²天津中医药大学　中药学院，天津　301617

通讯作者:

王萌，女，副研究员，硕士生导师，研究方向：中药生物药剂学研究、中药制剂稳定性研究。E-mail：mengwangr@163.com。

陈美玲，女，副教授，硕士生导师，研究方向：纳米材料在生命科学中的应用。E-mail：custard.chen@tjutcm.edu.cn。

作者简介:

宋佳敏，女，硕士研究生在读，研究方向：新型制剂制备与研究。E-mail：15122833075@139.com。

Application of high content analysis in the study of toxicity mechanism of drug mitochondrial damage

Jia-min SONG¹, Meng WANG¹, Mei-ling CHEN²

Affiliations

¹Tianjin University of Traditional Chinese Medicine State Key Laboratory of Component-based Chinese Medicine, Tianjin 301617, China

²Tianjin University of Traditional Chinese Medicine School of Chinese Materia Medica, Tianjin 301617, China

出版时间: 2021-10-30

文章导航

摘要

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高内涵技术是一种细胞成像技术，利用其自动显微镜、荧光探针及图像分析系统可以同时获得细胞的多项参数，具有高效、快速、多指标的优点，目前已经逐渐用于多种药物细胞毒性的分析和筛选。本文综述了近年来高内涵技术在研究药物对肝脏、肾脏、神经等组织线粒体损伤机制中的应用，旨为进一步药物毒性研究及安全用药提供参考。

关键词

高内涵分析 / 高内涵筛选 / 药物毒性 / 线粒体损伤 / 毒性机制

Abstract

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High content technology is a kind of cell imaging technology, which can obtain many parameters of cells simultaneously by using its automatic microscope, fluorescence probe and image analysis system. It has the advantages of high efficiency, high speed and multi index, and has been gradually used in the analysis and screening of various drug cytotoxicity. This paper reviews the application of high content technology in the study of mitochondrial damage mechanism of liver, kidney, nerve and other tissues in recent years, aiming to provide reference for further drug toxicity research and safe drug use.

Key words

high content analysis / high content screening / drug toxicity / mitochondrial damage / mechanisms of toxicity

引用本文

宋佳敏, 王萌, 陈美玲. 高内涵技术在药物线粒体损伤毒性机制研究中的应用. 中国新药杂志, 2021 , 30 (20) : 1861 -1866 .

Jia-min SONG, Meng WANG, Mei-ling CHEN. Application of high content analysis in the study of toxicity mechanism of drug mitochondrial damage[J]. Chinese Journal of New Drugs, 2021 , 30 (20) : 1861 -1866 .

基金

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国家自然科学基金面上项目(81873191)

参考文献

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2021年第30卷第20期

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首发时间：2026-10-30
出版时间：2021-10-30

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录用日期：2021-06-25

基金

国家自然科学基金面上项目(81873191)

作者信息

¹天津中医药大学　组分中药国家重点实验室，天津　301617

²天津中医药大学　中药学院，天津　301617

通讯作者:

王萌，女，副研究员，硕士生导师，研究方向：中药生物药剂学研究、中药制剂稳定性研究。E-mail：mengwangr@163.com。

陈美玲，女，副教授，硕士生导师，研究方向：纳米材料在生命科学中的应用。E-mail：custard.chen@tjutcm.edu.cn。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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