Article(id=1322627667015352411, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1322627622589280278, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=1584979200000, acceptedDateStr=2020-03-24, onlineDate=1793268609049, onlineDateStr=2026-10-29, pubDate=1617033600000, pubDateStr=2021-03-30, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1793268609049, onlineIssueDateStr=2026-10-29, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1793268609049, creator=13701087609, updateTime=1793268609049, updator=13701087609, issue=Issue{id=1322627622589280278, tenantId=1146029695717560320, journalId=1235980733773295621, year='2021', volume='30', issue='6', pageStart='481', pageEnd='576', issueExtLink='null', onlineDate='null', pubDate='1617033600000', pubDateStr='2021-03-30', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1793268598458, creator='13701087609', updateTime=1793269229249, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1322630268373021321, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1322627622589280278, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1322630268373021322, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1322627622589280278, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=562, endPage=568, ext={EN=ArticleExt(id=1322627667170541660, articleId=1322627667015352411, tenantId=1146029695717560320, journalId=1235980733773295621, language=EN, title=Isolation, purification and mass spectral analysis of recombinant Arg
34-GLP-1 [7-37], columnId=null, journalTitle=Chinese Journal of New Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=
Objective: To identify the potential impurities generated during manufacturing of recombinant Arg34-GLP-1 [7-37].
Methods: After fermentation, the broth extract was analyzed by HPLC, then the main product Arg34-GLP-1 [7-37] and impurities were separated and purified by ion exchange chromotography and preparative HPLC respectively. The main product was treated by extreme conditions such as high temperature, acid and alkali experiments in order to figure out how the impurities were generated. MALDI-TOF, protein sequencing and other methods were used to identify the structures of impurities.
Results: The purity of the main product reached 97.9% after purification by anion exchange chromatography and preparation of liquid phase. Three main impurities were isolated and purified to 91.4%, 99.2%, and 96.7%. Their MWs of [M+H]+ were 1 033.544 6, 2 744.239 7, and 3 112.447 5 as determined by MALDI-TOF. Through the destructive test and the amino acid sequences of Arg34-GLP-1 [7-37] it was speculated that the possible sequences of impurity 1, impurity 3, and impurity 9 may be E-F-I-A-W-L-V-R, H-A-E-G-T-F-T-S-D-V-S-S-Y-L-E-G-Q-A-A-K-E-F-I-A-W, H-A-E-G-T-F-T-S-D-V-S-S-Y-L-E-G-Q-A-A-K-E-F-I-A-W-L-V-R. Comparison of the components before and after purification showed that the above three main impurities were generated by the fermentation process, and the contents of these impurities would increase significantly by extreme conditions. The structure analysis and mechanism investigation of the three impurities analyzed in this study were all published for the first time.
Conclusion: Since recombinant Arg34-GLP-1 [7-37] is unstable in high temperature, peracid and alkali, and oxidizing environments, care should be taken to avoid these conditions in order to prevent degradation of the main product during purification. This paper shows some pitfalls to avoid for the research of GLP-1 and its analogs peptides.
, authors=Tao LIANG, Ting GAO, Jin-jun LI, Chen ZHAO, Yan GE, Duan-hua LI, Lu WANG, authorsList=Tao LIANG, Ting GAO, Jin-jun LI, Chen ZHAO, Yan GE, Duan-hua LI, Lu WANG, authorCompany=null, correspAuthors=Lu WANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1322627667774521437, articleId=1322627667015352411, tenantId=1146029695717560320, journalId=1235980733773295621, language=CN, title=重组Arg
34-GLP-1[7-37]杂质的分离纯化及质谱解析, columnId=1236330556003242277, journalTitle=中国新药杂志, columnName=实验研究, runingTitle=null, highlight=null, articleAbstract=
目的： 对重组Arg34-胰高血糖素样肽-1（Arg34-Glucagon-like Peptide-1[7-37]，Arg34-GLP-1[7-37]）生产制备过程中的潜在杂质进行分离纯化和结构确证。
方法： 将发酵后的料液进行高效液相色谱分析检测，利用离子交换色谱和制备液相纯化得到Arg34-GLP-1[7-37]纯品和杂质组分，使用纯品进行高温、酸碱等不同条件下的破坏实验，来探知杂质产生机理，利用MALDI-TOF质谱、蛋白质全序列测序等方式推断杂质的分子量与结构。
结果： 经阴离子交换色谱及反相制备纯化后，Arg34-GLP-1[7-37]的纯度可达97.9%。分离出3种主要杂质并将其纯化至91.4%，99.2%和96.7%。基质辅助激光解吸电离飞行时间质谱（Matrix-assisted laser desorption/ionization time of flight mass spectrometry，MALDI-TOF）测试其[M+H]+分子量分别为1 033.544 6，2 744.239 7和3 112.447 5，通过破坏实验及Arg34-GLP-1[7-37]的氨基酸序列来推测杂质1、杂质3与杂质9可能序列分别为E-F-I-A-W-L-V-R，H-A-E-G-T-F-T-S-D-V-S-S-Y-L-E-G-Q-A-A-K-E-F-I-A-W，H-A-E-G-T-F-T-S-D-V-S-S-Y-L-E-G-Q-A-A-K-E-F-I-A-W-L-V-R。将纯化前后的成分进行比对，表明上述3个主要杂质是由发酵过程产生，通过极端条件处理这些杂质，其含量会显著增加。本研究解析的3个杂质结构及机理探究均为首次发表。
结论： 由于重组Arg34-GLP-1[7-37]在高温、过酸过碱及氧化环境中不稳定，为防止纯化过程中主产物降解，应注意避免这些条件。本研究为胰高血糖素样肽-1（glucagon-like peptide1，GLP-1）及其类似物的研究指出了一些应避免的误区。
, authors=梁涛, 高婷, 李进军, 赵晨, 葛燕, 李端华, 王辂, authorsList=梁涛, 高婷, 李进军, 赵晨, 葛燕, 李端华, 王辂, authorCompany=null, correspAuthors=王辂, authorNote=
, correspAuthorsNote=
, copyrightStatement=null, copyrightOwner=《中国新药杂志》有限公司, extLink=null, articleAbsUrl=null, sourceXml=7iVNV+GZa1N+nAy7WAvlhg==, magXml=uFSBSDs70To1jqHxsw05pQ==, pdfUrl=null, pdf=iBOuQ9jrcCie6cFV8fekWQ==, pdfFileSize=1266023, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=+W9BCN71derH7KdH6730KQ==, mapNumber=null, fund=null)}, authors=[Author(id=1322627668210729058, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=1369327437@qq.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322627668282032228, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627668210729058, language=EN, stringName=Tao LIANG, firstName=Tao, middleName=null, lastName=LIANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322627668395278437, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627668210729058, language=CN, stringName=梁涛, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=成都大学四川抗菌素工业研究所，成都 610052, bio={"content":"
梁涛，男，硕士研究生，主要从事蛋白与多肽类药物分离纯化研究。联系电话：（028）84216031，E-mail：1369327437@qq.com。
"}, bioImg=null, bioContent=
梁涛，男，硕士研究生，主要从事蛋白与多肽类药物分离纯化研究。联系电话：（028）84216031，E-mail：1369327437@qq.com。
, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322627668135231582, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, xref=null, ext=[AuthorCompanyExt(id=1322627668143620191, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China), AuthorCompanyExt(id=1322627668152008800, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=成都大学四川抗菌素工业研究所，成都 610052)])]), Author(id=1322627668458192999, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322627668525301865, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627668458192999, language=EN, stringName=Ting GAO, firstName=Ting, middleName=null, lastName=GAO, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322627668584022122, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627668458192999, language=CN, stringName=高婷, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=成都大学四川抗菌素工业研究所，成都 610052, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322627668135231582, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, xref=null, ext=[AuthorCompanyExt(id=1322627668143620191, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China), AuthorCompanyExt(id=1322627668152008800, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=成都大学四川抗菌素工业研究所，成都 610052)])]), Author(id=1322627668655325292, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322627668739211374, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627668655325292, language=EN, stringName=Jin-jun LI, firstName=Jin-jun, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322627668827291759, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627668655325292, language=CN, stringName=李进军, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=成都大学四川抗菌素工业研究所，成都 610052, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322627668135231582, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, xref=null, ext=[AuthorCompanyExt(id=1322627668143620191, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China), AuthorCompanyExt(id=1322627668152008800, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=成都大学四川抗菌素工业研究所，成都 610052)])]), Author(id=1322627668894400625, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322627668969898099, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627668894400625, language=EN, stringName=Chen ZHAO, firstName=Chen, middleName=null, lastName=ZHAO, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322627669057978484, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627668894400625, language=CN, stringName=赵晨, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=成都大学四川抗菌素工业研究所，成都 610052, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322627668135231582, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, xref=null, ext=[AuthorCompanyExt(id=1322627668143620191, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China), AuthorCompanyExt(id=1322627668152008800, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=成都大学四川抗菌素工业研究所，成都 610052)])]), Author(id=1322627669116698742, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322627669234139256, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627669116698742, language=EN, stringName=Yan GE, firstName=Yan, middleName=null, lastName=GE, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322627669305442425, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627669116698742, language=CN, stringName=葛燕, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=成都大学四川抗菌素工业研究所，成都 610052, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322627668135231582, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, xref=null, ext=[AuthorCompanyExt(id=1322627668143620191, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China), AuthorCompanyExt(id=1322627668152008800, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=成都大学四川抗菌素工业研究所，成都 610052)])]), Author(id=1322627669372551291, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, orderNo=5, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322627669456437373, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627669372551291, language=EN, stringName=Duan-hua LI, firstName=Duan-hua, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322627669544517758, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627669372551291, language=CN, stringName=李端华, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=成都大学四川抗菌素工业研究所，成都 610052, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322627668135231582, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, xref=null, ext=[AuthorCompanyExt(id=1322627668143620191, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China), AuthorCompanyExt(id=1322627668152008800, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=成都大学四川抗菌素工业研究所，成都 610052)])]), Author(id=1322627669615820928, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, orderNo=6, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=wanglu@cdu.edu.cn, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1322627669703901314, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627669615820928, language=EN, stringName=Lu WANG, firstName=Lu, middleName=null, lastName=WANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322627669771010179, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, authorId=1322627669615820928, language=CN, stringName=王辂, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=成都大学四川抗菌素工业研究所，成都 610052, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322627668135231582, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, xref=null, ext=[AuthorCompanyExt(id=1322627668143620191, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China), AuthorCompanyExt(id=1322627668152008800, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=成都大学四川抗菌素工业研究所，成都 610052)])])], keywords=[Keyword(id=1322627669863284868, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, language=EN, orderNo=1, keyword=impurity profile), Keyword(id=1322627669951365253, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, language=EN, orderNo=2, keyword=recombinant Arg
34-GLP-1 [7-37]), Keyword(id=1322627670035251334, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, language=EN, orderNo=3, keyword=peptide purification), Keyword(id=1322627670278520967, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, language=EN, orderNo=4, keyword=high resolution mass spectrometry), Keyword(id=1322627670412738696, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, language=CN, orderNo=1, keyword=杂质谱), Keyword(id=1322627670718922889, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, language=CN, orderNo=2, keyword=重组Arg
34-GLP-1[7-37]), Keyword(id=1322627670781837450, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, language=CN, orderNo=3, keyword=多肽纯化), Keyword(id=1322627671075438731, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, language=CN, orderNo=4, keyword=高分辨质谱)], refs=[Reference(id=1322627671952048271, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=null, journalName=null, refType=null, unstructuredReference=陆珣靓, 李成江. GLP-1的生理作用及其机制研究进展[J]. 浙江医学, 2011, 33(1): 137-140., articleTitle=null, refAbstract=null), Reference(id=1322627672069488784, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=null, journalName=null, refType=null, unstructuredReference=吴婷婷, 李小兵, 张兰青. GLP-1受体激动剂联合阿卡波糖治疗T2DM临床效果及其影响[J]. 浙江临床医学, 2018, 20(4): 626-628., articleTitle=null, refAbstract=null), Reference(id=1322627672325341329, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=null, journalName=null, refType=null, unstructuredReference=王小平, 容蓉, 田景振. 多肽类药物分析方法的研究进展[J]. 食品与药品, 2009, 11(9): 55-58., articleTitle=null, refAbstract=null), Reference(id=1322627673919176851, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=null, journalName=null, refType=null, unstructuredReference=FARILLA L, BULOTTA A, HIRSHBERG B,
et al. Glucagon-like peptide 1 inhibits cell apoptosis and improves glucose responsiveness of freshly isolated human islets[J].
Endocrinology, 2003, 144(12): 5149-5158., articleTitle=null, refAbstract=null), Reference(id=1322627674007257236, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=null, journalName=null, refType=null, unstructuredReference=SANZNEBOT V, BENAVENTE F, TORO I,
et al. Separation and characterization of complex crude mixtures produced in the synthesis of therapeutic peptide hormones by liquid chromatography coupled to electrospray mass spectrometry (LC-ES-MS)[J].
Anal Chimica Acta, 2004, 521(1): 25-36., articleTitle=null, refAbstract=null), Reference(id=1322627674091143317, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=null, journalName=null, refType=null, unstructuredReference=井绪文, 吴优, 李柏林, 等. 高效液相色谱法测定扎那米韦鼻用粉雾剂含量和相关杂质的研究[J]. 中国新药杂志, 2018, 27(20): 2447-2450., articleTitle=null, refAbstract=null), Reference(id=1322627674175029398, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=null, journalName=null, refType=null, unstructuredReference=周冠月. 清开灵注射液中多肽含量的快速测定及杂质蛋白的限量检查[D]. 北京: 北京中医药大学, 2011., articleTitle=null, refAbstract=null), Reference(id=1322627674237943959, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=null, journalName=null, refType=null, unstructuredReference=D'HONDT M, BRACKE N, TAEVERNIER L,
et al. Related impurities in peptide medicines[J].
J Pharm Biomed Anal, 2014, 101: 2-30., articleTitle=null, refAbstract=null)], funds=[Fund(id=1322627671180296332, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, awardId=20GJHZ0211, language=CN, fundingSource=四川省科学技术厅国际合作资助项目(20GJHZ0211), fundOrder=null, country=null), Fund(id=1322627671507452045, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, awardId=2016-XT00-00023-GX, language=CN, fundingSource=成都市科技局基金资助项目(2016-XT00-00023-GX), fundOrder=null, country=null), Fund(id=1322627671587143822, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, awardId=2020YFH0013, language=CN, fundingSource=国际科技创新合作资助项目(2020YFH0013), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1322627668135231582, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, xref=null, ext=[AuthorCompanyExt(id=1322627668143620191, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, China), AuthorCompanyExt(id=1322627668152008800, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322627667015352411, companyId=1322627668135231582, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=成都大学四川抗菌素工业研究所，成都 610052)])], figs=null, attaches=null, journal=Journal(id=1235980159023624194, delFlag=0, nameCn=中国新药杂志, nameEn=Chinese Journal of New Drugs, nameHistory1=null, nameHistory2=null, issn=1003-3734, eissn=null, cn=11-2850/R, coden=ZXZHA6, periodic=3, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=ohPEEN//Vz1tDT3ZBNTp4Q==, journalPrice=null, startedYear=null, abbrevIsoEn=Chinese Journal of New Drugs, journalRemark=null, publicationField=null, createdTime=1772610233314, updatedTime=1788948968376, createdBy=18614031015, updatedBy=13041195026, firstLetterCn=Z, firstLetterEn=Z, subjectCode=Medical and Pharmaceutical Sciences, subjectName=null, subjectCodeEn=Medical and Pharmaceutical Sciences, subjectNameEn=null, picCn=ohPEEN//Vz1tDT3ZBNTp4Q==, picEn=f9WK9bvTM4guTmS/iGhOMg==, jcr=null, cjcr=null, exts=[JournalExt(id=1304509780974924039, language=CN, name=中国新药杂志, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788948968404, updatedTime=1788948968404, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=http://draft.newdrug.cn/journalx_zgxy/authorLogOn.action, submissionEditorUrl=http://draft.newdrug.cn/journalx_zgxy/editorLogOn.action, submissionReviewUrl=http://draft.newdrug.cn/journalx_zgxy/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1304509781025255688, language=EN, name=Chinese Journal of New Drugs, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788948968416, updatedTime=1788948968416, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=http://draft.newdrug.cn/journalx_zgxy/authorLogOn.action, submissionEditorUrl=http://draft.newdrug.cn/journalx_zgxy/editorLogOn.action, submissionReviewUrl=http://draft.newdrug.cn/journalx_zgxy/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1235980733773295621, websiteList=[Website(id=1235982099191222761, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1235980733773295621, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgxyzz/CN, language=CN, createTime=1772610695884, createBy=18614031015, updateTime=1772610860397, updateBy=18614031015, name=中国新药杂志-中文, tplId=1146099689490845704, title=中国新药杂志, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1235983918629966444, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=articleTextType, value=kx, createTime=1772611129672, updateTime=1772611129672, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918604800617, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=banner, value=null, createTime=1772611129666, updateTime=1772611129666, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918655132271, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=grayFlag, value=0, createTime=1772611129678, updateTime=1772611129678, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918596412008, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=logo, value=https://castjournals.cast.org.cn/joweb/zgxyzz/CN/file/pic?fileId=W567vm71kFC7UhK9TSU6xw==, createTime=1772611129664, updateTime=1772611129664, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918667715185, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=minRunFlag, value=0, createTime=1772611129681, updateTime=1772611129681, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918617383531, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgxyzz/CN/file/pic, createTime=1772611129669, updateTime=1772611129669, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918659326576, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=silenceFlag, value=0, createTime=1772611129679, updateTime=1772611129679, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918613189226, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1772611129668, updateTime=1772611129668, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918634160749, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=themeColor, value=null, createTime=1772611129673, updateTime=1772611129673, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918646743662, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=themeStyle, value=null, createTime=1772611129676, updateTime=1772611129676, creator=18614031015, updator=18614031015)]), Website(id=1235982099270914542, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1235980733773295621, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgxyzz/EN, language=EN, createTime=1772610695903, createBy=18614031015, updateTime=1772610875891, updateBy=18614031015, name=中国新药杂志-英文, tplId=1146101810881728533, title=Chinese Journal of New Drugs, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1235983955829248630, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=articleTextType, value=kx, createTime=1772611138541, updateTime=1772611138541, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955804082803, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=banner, value=null, createTime=1772611138535, updateTime=1772611138535, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955854414457, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=grayFlag, value=0, createTime=1772611138547, updateTime=1772611138547, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955795694194, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=logo, value=https://castjournals.cast.org.cn/joweb/zgxyzz/EN/file/pic?fileId=W567vm71kFC7UhK9TSU6xw==, createTime=1772611138533, updateTime=1772611138533, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955866997371, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=minRunFlag, value=0, createTime=1772611138550, updateTime=1772611138550, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955820860021, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgxyzz/EN/file/pic, createTime=1772611138539, updateTime=1772611138539, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955862803066, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=silenceFlag, value=0, createTime=1772611138549, updateTime=1772611138549, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955812471412, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1772611138537, updateTime=1772611138537, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955837637239, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=themeColor, value=null, createTime=1772611138543, updateTime=1772611138543, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955846025848, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=themeStyle, value=null, createTime=1772611138545, updateTime=1772611138545, creator=18614031015, updator=18614031015)])], journalTitle=中国新药杂志, weixinUrl=null, journalUrl=http://www.newdrug.cn/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Chinese Journal of New Drugs, journalPhotoCn=ohPEEN//Vz1tDT3ZBNTp4Q==, journalPhotoEn=f9WK9bvTM4guTmS/iGhOMg==, journalFirstLetter=Z, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/zgxyzz/CN/Y2021/V30/I6/562, detailUrlEn=https://castjournals.cast.org.cn/joweb/zgxyzz/EN/Y2021/V30/I6/562, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zgxyzz/CN/PDF/Y2021/V30/I6/562, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zgxyzz/EN/PDF/Y2021/V30/I6/562, aliStartDate=0, aliEndDate=0, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1617033600000, fullTextJson=null, articleText=null, reference=null)
全屏
文章导航
PDF下载
11
2
关闭全屏
北京市海淀区学院南路86号
010-62662699（运营及技术支持）
qkjq@cast.org.cn
