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Article(id=1322627661197857384, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1322627622589280278, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=1595174400000, acceptedDateStr=2020-07-20, onlineDate=1793268607662, onlineDateStr=2026-10-29, pubDate=1617033600000, pubDateStr=2021-03-30, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1793268607662, onlineIssueDateStr=2026-10-29, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1793268607662, creator=13701087609, updateTime=1793268607662, updator=13701087609, issue=Issue{id=1322627622589280278, tenantId=1146029695717560320, journalId=1235980733773295621, year='2021', volume='30', issue='6', pageStart='481', pageEnd='576', issueExtLink='null', onlineDate='null', pubDate='1617033600000', pubDateStr='2021-03-30', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1793268598458, creator='13701087609', updateTime=1793269229249, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1322630268373021321, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1322627622589280278, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1322630268373021322, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1322627622589280278, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=514, endPage=519, ext={EN=ArticleExt(id=1322627661378212457, articleId=1322627661197857384, tenantId=1146029695717560320, journalId=1235980733773295621, language=EN, title=Comparative study of immune responses induced by MDCK cell-based and egg-based influenza vaccines in mice, columnId=null, journalTitle=Chinese Journal of New Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=
Objective:

To compare the difference of immune response induced by egg-based and cell-based H1N1 influenza vaccines.

Methods:

C57BL/6 mice were injected subcutaneously with H1N1 influenza vaccines produced from eggs or MDCK cells, respectively. The cellular and humoral immune responses induced by two type of vaccines were detected by using methods such as serological test, intracellular cytokine staining and multiplex cytokine detection assay.

Results:

In terms of humoral immunity, the serum titer of the cellular vaccine group was basically the same as that of the chicken embryo vaccine group. As to cellular immunity, the secretion level of immune-related cytokines in the cellular vaccine group was more significant.

Conclusion:

Due to the different culture media, the two monovalent influenza vaccines have certain differences in the induction of immune response. Cellular vaccine has the advantage of inducing multiple immune responses, and the combination of other advantages of cellular technology indicates that the use of cellular technology will become a trend to produce influenza vaccine. This study is helpful to evaluate the immune level in mice induced by monovalent influenza vaccines prepared with different substrates.

, authors=Zhe-gang ZHANG1, 2, Xing-hang LI2, Jia-you ZHANG1, Wei ZHAO2, Chen LIU2, Tian HAN2, Lu-yao YAN1, Zhi-wu XIA2, Chang-gui LI3, Xiao-ming YANG1, 4, authorsList=Zhe-gang ZHANG, Xing-hang LI, Jia-you ZHANG, Wei ZHAO, Chen LIU, Tian HAN, Lu-yao YAN, Zhi-wu XIA, Chang-gui LI, Xiao-ming YANG, authorCompany=null, correspAuthors=Chang-gui LI, Xiao-ming YANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1322627661579539050, articleId=1322627661197857384, tenantId=1146029695717560320, journalId=1235980733773295621, language=CN, title=MDCK细胞基质与鸡胚基质单价流感疫苗在小鼠中诱导的免疫反应的比较研究, columnId=1236330552693944345, journalTitle=中国新药杂志, columnName=生物医药前沿, runingTitle=null, highlight=null, articleAbstract=
目的：

比较鸡胚基质和细胞基质的H1N1单价流感疫苗在小鼠体内诱导的免疫反应的差异。

方法：

分别采用鸡胚基质和MDCK细胞基质H1N1流感疫苗注射C57BL/6小鼠，通过体外中和实验，胞内细胞因子染色及多细胞因子检测比较2种基质生产的单价流感疫苗诱导的体液及细胞免疫反应的差异。

结果：

体液免疫方面，细胞基质疫苗组血清滴度与鸡胚基质疫苗组血清滴度基本一致。在细胞免疫方面，细胞基质疫苗组的细胞免疫相关细胞因子分泌水平更加显著。

结论：

由于病毒培养基质的不同，2种流感单价疫苗在诱导免疫反应时间存在一定差异。细胞疫苗具有诱导多种免疫反应的优势，综合细胞工艺的其他优势，说明利用细胞工艺生产流感疫苗将会成为趋势。本实验有助于评价不同基质制备的单价流感疫苗在小鼠中诱导的免疫水平。

, authors=张哲罡1, 2, 李兴航2, 张家友1, 赵巍2, 刘琛2, 韩天2, 闫璐瑶1, 夏志武2, 李长贵3, 杨晓明1, 4, authorsList=张哲罡, 李兴航, 张家友, 赵巍, 刘琛, 韩天, 闫璐瑶, 夏志武, 李长贵, 杨晓明, authorCompany=null, correspAuthors=李长贵, 杨晓明, authorNote=

张哲罡，男，博士研究生，主要从事病毒性疫苗研发。联系电话：（027）86637107，E-mail：。

李兴航，男，硕士研究生，主要从事病毒疫苗研究。联系电话：（027）88843621，E-mail：。

, correspAuthorsNote=
李长贵，男，研究员，主要从事疫苗的开发、检定及管理工作。联系电话：（010）53851616，E-mail：。
杨晓明，男，研究员，主要从事疫苗的开发与管理工作。联系电话：（010）84663377，E-mail：。
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张哲罡，男，博士研究生，主要从事病毒性疫苗研发。联系电话：（027）86637107，E-mail：。

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李兴航，男，硕士研究生，主要从事病毒疫苗研究。联系电话：（027）88843621，E-mail：。

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MDCK细胞基质与鸡胚基质单价流感疫苗在小鼠中诱导的免疫反应的比较研究
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张哲罡 1, 2 , 李兴航 2 , 张家友 1 , 赵巍 2 , 刘琛 2 , 韩天 2 , 闫璐瑶 1 , 夏志武 2 , 李长贵 3 , 杨晓明 1, 4
中国新药杂志 | 生物医药前沿 2021,30(6): 514-519
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中国新药杂志 |生物医药前沿 2021 , 30 (6) : 514 -519
MDCK细胞基质与鸡胚基质单价流感疫苗在小鼠中诱导的免疫反应的比较研究
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张哲罡1, 2 , 李兴航2 , 张家友1, 赵巍2, 刘琛2, 韩天2, 闫璐瑶1, 夏志武2, 李长贵3 , 杨晓明1, 4
作者信息
  • 1国家联合疫苗工程技术研究中心，武汉　430207
  • 2武汉生物制品研究所有限责任公司，武汉　430207
  • 3中国食品药品检定研究院，北京　100050
  • 4中国生物技术股份有限公司，北京　100029
通讯作者:
李长贵，男，研究员，主要从事疫苗的开发、检定及管理工作。联系电话：（010）53851616，E-mail：。
杨晓明，男，研究员，主要从事疫苗的开发与管理工作。联系电话：（010）84663377，E-mail：。
作者简介:

张哲罡，男，博士研究生，主要从事病毒性疫苗研发。联系电话：（027）86637107，E-mail：。

李兴航，男，硕士研究生，主要从事病毒疫苗研究。联系电话：（027）88843621，E-mail：。

Comparative study of immune responses induced by MDCK cell-based and egg-based influenza vaccines in mice
Zhe-gang ZHANG1, 2 , Xing-hang LI2 , Jia-you ZHANG1, Wei ZHAO2, Chen LIU2, Tian HAN2, Lu-yao YAN1, Zhi-wu XIA2, Chang-gui LI3 , Xiao-ming YANG1, 4
Affiliations
  • 1Wuhan Institute of Biological Products Co., Ltd., Wuhan 430207, China
  • 2National Institute of Engineering Technology Research in Combination Vaccines, Wuhan 430207, China
  • 3National Institute for Food and Drug Control, Beijing 100050, China
  • 4China National Biotec Group, Beijing 100029, China
出版时间: 2021-03-30
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摘要
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目的：

比较鸡胚基质和细胞基质的H1N1单价流感疫苗在小鼠体内诱导的免疫反应的差异。

方法：

分别采用鸡胚基质和MDCK细胞基质H1N1流感疫苗注射C57BL/6小鼠，通过体外中和实验，胞内细胞因子染色及多细胞因子检测比较2种基质生产的单价流感疫苗诱导的体液及细胞免疫反应的差异。

结果：

体液免疫方面，细胞基质疫苗组血清滴度与鸡胚基质疫苗组血清滴度基本一致。在细胞免疫方面，细胞基质疫苗组的细胞免疫相关细胞因子分泌水平更加显著。

结论：

由于病毒培养基质的不同，2种流感单价疫苗在诱导免疫反应时间存在一定差异。细胞疫苗具有诱导多种免疫反应的优势，综合细胞工艺的其他优势，说明利用细胞工艺生产流感疫苗将会成为趋势。本实验有助于评价不同基质制备的单价流感疫苗在小鼠中诱导的免疫水平。

H1N1流感疫苗  /  MDCK细胞基质流感疫苗  /  鸡胚基质流感疫苗  /  免疫效果
Objective:

To compare the difference of immune response induced by egg-based and cell-based H1N1 influenza vaccines.

Methods:

C57BL/6 mice were injected subcutaneously with H1N1 influenza vaccines produced from eggs or MDCK cells, respectively. The cellular and humoral immune responses induced by two type of vaccines were detected by using methods such as serological test, intracellular cytokine staining and multiplex cytokine detection assay.

Results:

In terms of humoral immunity, the serum titer of the cellular vaccine group was basically the same as that of the chicken embryo vaccine group. As to cellular immunity, the secretion level of immune-related cytokines in the cellular vaccine group was more significant.

Conclusion:

Due to the different culture media, the two monovalent influenza vaccines have certain differences in the induction of immune response. Cellular vaccine has the advantage of inducing multiple immune responses, and the combination of other advantages of cellular technology indicates that the use of cellular technology will become a trend to produce influenza vaccine. This study is helpful to evaluate the immune level in mice induced by monovalent influenza vaccines prepared with different substrates.

H1N1 influenza vaccines  /  MDCK cell-based influenza vaccine  /  egg-based influenza vaccine  /  immunogenicity
张哲罡, 李兴航, 张家友, 赵巍, 刘琛, 韩天, 闫璐瑶, 夏志武, 李长贵, 杨晓明. MDCK细胞基质与鸡胚基质单价流感疫苗在小鼠中诱导的免疫反应的比较研究. 中国新药杂志, 2021 , 30 (6) : 514 -519 .
Zhe-gang ZHANG, Xing-hang LI, Jia-you ZHANG, Wei ZHAO, Chen LIU, Tian HAN, Lu-yao YAN, Zhi-wu XIA, Chang-gui LI, Xiao-ming YANG. Comparative study of immune responses induced by MDCK cell-based and egg-based influenza vaccines in mice[J]. Chinese Journal of New Drugs, 2021 , 30 (6) : 514 -519 .

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2021年第30卷第6期
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  • 首发时间：2026-10-29
  • 出版时间：2021-03-30
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  • 录用日期：2020-07-20
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    1国家联合疫苗工程技术研究中心，武汉　430207
    2武汉生物制品研究所有限责任公司，武汉　430207
    3中国食品药品检定研究院，北京　100050
    4中国生物技术股份有限公司，北京　100029

通讯作者:

李长贵，男，研究员，主要从事疫苗的开发、检定及管理工作。联系电话：（010）53851616，E-mail：。
杨晓明，男，研究员，主要从事疫苗的开发与管理工作。联系电话：（010）84663377，E-mail：。
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2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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