Article(id=1322626703386592119, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1322626690153562922, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=1582300800000, acceptedDateStr=2020-02-22, onlineDate=1793268379303, onlineDateStr=2026-10-29, pubDate=1610640000000, pubDateStr=2021-01-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1793268379303, onlineIssueDateStr=2026-10-29, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1793268379303, creator=13701087609, updateTime=1793268379303, updator=13701087609, issue=Issue{id=1322626690153562922, tenantId=1146029695717560320, journalId=1235980733773295621, year='2021', volume='30', issue='1', pageStart='1', pageEnd='93', issueExtLink='null', onlineDate='null', pubDate='1610640000000', pubDateStr='2021-01-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1793268376148, creator='13701087609', updateTime=1793269030229, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1322629433626837820, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1322626690153562922, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1322629433626837821, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1322626690153562922, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=73, endPage=79, ext={EN=ArticleExt(id=1322626703575335800, articleId=1322626703386592119, tenantId=1146029695717560320, journalId=1235980733773295621, language=EN, title=Development of standard reagents for heparin anti-FXa and anti-F Ⅱa assay, columnId=null, journalTitle=Chinese Journal of New Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=
Objective: To develop the first batch of standardized reagents for the potency assay of heparin, low-molecular-weight heparin (LMWH) anti-factor Xa and anti-factor Ⅱa.
Methods: Fibrinogen coagulation method was used for factor Ⅱa reagent to assign the potency, which was calculated with the national standard of thrombin. Chromogenic substrate method was used for antithrombin Ⅲ to assign the potency, which was calculated with the WHO international standard. The potency of facor Xa was measured using a fully automatic hemagglutination instrument, calculated with the national standard of human coagulation factor. The purity and free pNA content of chromogenic substrates S-2765 and S-2238 were analyzed by reversed-phase chromatography. The separation was obtained by the column Agela Technologies Venusil XBP RP C18 (250 mm×4.6 mm, 5 μm) using mobile phase A of 20% acetonitrile solution which contained 0.1% trifluoroacetic acid and phase B of 60% acetonitrile solution which contained 0.1% trifluoroacetic acid in a gradient elution. The wavelength was set at 313 nm. The constant Km was calculated to evaluate the enzyme and substrate affinities.
Results: The standard reagent potency of factors Ⅱa, Factor Xa, and Antithrombin III were 40 IU, 76.3 nKat, and 10 IU per vial, respectively. The limit of quantification (LOQ) and detection limit of detection (LOD) of pNA were 7.6 ng·mL-1 and 2.3 ng·mL-1; the linear range is 0.005~100 μg·mL-1(r=1.0); the sample recovery rate is in the range of 98.5%~102.3%. The contents of chromogenic substrates, S-2765 and S-2238, are 96.2% and 97.3%. There is no statistical difference in Km between imported reagents and standard reagents.
Conclusion: According to the quality test results of standard reagents in this study, it can be seen that standard reagents can completely replace imported reagents, and the value of standard reagents is much accurate compared to imported reagents.
, authors=Yue WANG, Jing LI, Xin-yu WANG, Li-sha LIU, Hui-hong FAN, authorsList=Yue WANG, Jing LI, Xin-yu WANG, Li-sha LIU, Hui-hong FAN, authorCompany=null, correspAuthors=Hui-hong FAN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1322626703906685817, articleId=1322626703386592119, tenantId=1146029695717560320, journalId=1235980733773295621, language=CN, title=肝素抗Xa因子和抗Ⅱa因子效价测定用标准试剂的研制, columnId=1236330556003242277, journalTitle=中国新药杂志, columnName=实验研究, runingTitle=null, highlight=null, articleAbstract=
目的： 研制我国首批肝素、低分子肝素抗Xa因子和抗Ⅱa因子效价测定用标准化试剂。
方法： Ⅱa因子试剂使用纤维蛋白原凝固法对效价进行赋值，效价溯源至凝血酶国家标准品；抗凝血酶Ⅲ采用生色底物法对效价进行赋值，效价溯源至WHO国际对照品；Xa因子效价采用全自动血凝仪进行测定，溯源至国家人凝血因子国家标准品；S-2765，S-2238生色底物和游离pNA含量采用反相色谱进行分离，色谱柱为Agela Technologies Venusil XBP RP C18（250 mm×4.6 mm，5 μm）；流动相A为20%乙腈与0.1%三氟醋酸溶液，流动相B为60%乙腈与0.1%三氟醋酸溶液；检测波长：313 nm；梯度洗脱；酶和底物亲和力通过米氏常数进行评价。
结果： Ⅱa因子、Xa因子、抗凝血酶每支效价结果分别为40 IU、76.3 nKat和10 IU；pNA的定量限与检出限分别为7.6和2.3 ng·mL-1；线性范围为0.005~100 μg·mL-1，r=1.0；加样回收率在98.5%~102.3%范围内；S-2765，S-2238发色底物含量分别为96.2%和97.3%；进口试剂与标准试剂米氏常数无统计学差异。
结论： 通过本研究对标准试剂较为全面的质量考察结果来看，标准试剂可完全替代进口试剂，且标准试剂效价赋值更为准确。
, authors=王悦, 李京, 王鑫钰, 刘莉莎, 范慧红, authorsList=王悦, 李京, 王鑫钰, 刘莉莎, 范慧红, authorCompany=null, correspAuthors=范慧红, authorNote=
, correspAuthorsNote=
, copyrightStatement=null, copyrightOwner=《中国新药杂志》有限公司, extLink=null, articleAbsUrl=null, sourceXml=up8t+udUuj/X5plLJ78r4g==, magXml=jznr0LYtEXknk0TOHQJXbw==, pdfUrl=null, pdf=S0SM6wQzL33aoM9XdfYlog==, pdfFileSize=1099040, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=9KZK2zLUjHVKDgmMS6av8g==, mapNumber=null, fund=null)}, authors=[Author(id=1322626705693459326, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=wangyue07@nifdc.org.cn, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322626705819288448, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, authorId=1322626705693459326, language=EN, stringName=Yue WANG, firstName=Yue, middleName=null, lastName=WANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=National Institute for Food & Drug Control, Beijing 102629, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322626705919951745, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, authorId=1322626705693459326, language=CN, stringName=王悦, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=中国食品药品检定研究院，北京 102629, bio={"content":"
王悦，女，副研究员，研究方向：多糖、酶类等生化药品质量控制。联系电话：（010）53851530，E-mail：wangyue07@nifdc.org.cn。
"}, bioImg=null, bioContent=
王悦，女，副研究员，研究方向：多糖、酶类等生化药品质量控制。联系电话：（010）53851530，E-mail：wangyue07@nifdc.org.cn。
, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322626705546658682, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, xref=null, ext=[AuthorCompanyExt(id=1322626705563435899, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, companyId=1322626705546658682, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=National Institute for Food & Drug Control, Beijing 102629, China), AuthorCompanyExt(id=1322626705571824508, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, companyId=1322626705546658682, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=中国食品药品检定研究院，北京 102629)])]), Author(id=1322626706008032131, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322626706163221381, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, authorId=1322626706008032131, language=EN, stringName=Jing LI, firstName=Jing, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=National Institute for Food & Drug Control, Beijing 102629, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322626706276467590, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, authorId=1322626706008032131, language=CN, stringName=李京, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=中国食品药品检定研究院，北京 102629, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322626705546658682, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, xref=null, ext=[AuthorCompanyExt(id=1322626705563435899, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, companyId=1322626705546658682, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=National Institute for Food & Drug Control, Beijing 102629, China), AuthorCompanyExt(id=1322626705571824508, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, companyId=1322626705546658682, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=中国食品药品检定研究院，北京 102629)])]), Author(id=1322626706351965064, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322626706456822666, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, authorId=1322626706351965064, language=EN, stringName=Xin-yu WANG, firstName=Xin-yu, middleName=null, lastName=WANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=National Institute for Food & Drug Control, Beijing 102629, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322626706540708747, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, authorId=1322626706351965064, language=CN, stringName=王鑫钰, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=中国食品药品检定研究院，北京 102629, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322626705546658682, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, xref=null, ext=[AuthorCompanyExt(id=1322626705563435899, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, companyId=1322626705546658682, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=National Institute for Food & Drug Control, Beijing 102629, China), AuthorCompanyExt(id=1322626705571824508, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, companyId=1322626705546658682, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=中国食品药品检定研究院，北京 102629)])]), Author(id=1322626706641372045, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322626706779784079, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, authorId=1322626706641372045, language=EN, stringName=Li-sha LIU, firstName=Li-sha, middleName=null, lastName=LIU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=National Institute for Food & Drug Control, Beijing 102629, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322626706905613200, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, authorId=1322626706641372045, language=CN, stringName=刘莉莎, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=中国食品药品检定研究院，北京 102629, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322626705546658682, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, xref=null, ext=[AuthorCompanyExt(id=1322626705563435899, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, companyId=1322626705546658682, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=National Institute for Food & Drug Control, Beijing 102629, China), AuthorCompanyExt(id=1322626705571824508, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, companyId=1322626705546658682, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=中国食品药品检定研究院，北京 102629)])]), Author(id=1322626706972722066, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=shenghuayaoshi@126.com, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1322626707236963220, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, authorId=1322626706972722066, language=EN, stringName=Hui-hong FAN, firstName=Hui-hong, middleName=null, lastName=FAN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=National Institute for Food & Drug Control, Beijing 102629, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322626707329237909, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, authorId=1322626706972722066, language=CN, stringName=范慧红, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=中国食品药品检定研究院，北京 102629, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322626705546658682, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, xref=null, ext=[AuthorCompanyExt(id=1322626705563435899, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, companyId=1322626705546658682, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=National Institute for Food & Drug Control, Beijing 102629, China), AuthorCompanyExt(id=1322626705571824508, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, companyId=1322626705546658682, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=中国食品药品检定研究院，北京 102629)])])], keywords=[Keyword(id=1322626707513787286, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, language=EN, orderNo=1, keyword=heparin), Keyword(id=1322626707627033495, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, language=EN, orderNo=2, keyword=low molecular weight heparin), Keyword(id=1322626707706725272, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, language=EN, orderNo=3, keyword=factor Xa), Keyword(id=1322626707811582873, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, language=EN, orderNo=4, keyword=factor Ⅱa), Keyword(id=1322626707887080346, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, language=EN, orderNo=5, keyword=antithrombin), Keyword(id=1322626707966772123, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, language=EN, orderNo=6, keyword=chromogenic substrate), Keyword(id=1322626708046463900, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, language=CN, orderNo=1, keyword=肝素), Keyword(id=1322626708126155677, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, language=CN, orderNo=2, keyword=低分子肝素), Keyword(id=1322626708205847454, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, language=CN, orderNo=3, keyword=Xa因子), Keyword(id=1322626709929706399, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, language=CN, orderNo=4, keyword=Ⅱa因子), Keyword(id=1322626710034564000, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, language=CN, orderNo=5, keyword=抗凝血酶), Keyword(id=1322626710101672865, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, language=CN, orderNo=6, keyword=发色底物)], refs=[Reference(id=1322626710353331107, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=null, journalName=null, refType=null, unstructuredReference=ONISHI A, ST ANGE K, DORDICK JS,
et al. Heparin and anticoagulation[J].
Front Biosci, 2016, 21(7): 1372-1392., articleTitle=null, refAbstract=null), Reference(id=1322626710449800100, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=null, journalName=null, refType=null, unstructuredReference=冯昭龙, 刘纯慧. 肝素前体heparosan的制备及药学应用研究进展[J]. 中国现代应用药学, 2019, 36(1): 114-119., articleTitle=null, refAbstract=null), Reference(id=1322626710529491877, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=null, journalName=null, refType=null, unstructuredReference=杨涛, 王春丽, 刘音, 等. 运用肝素酶血栓弹力图对等管腔容积肝素钠封管后凝血功能的评价[J]. 世界临床药物, 2015, 36(10): 679-681, 701., articleTitle=null, refAbstract=null), Reference(id=1322626710604989350, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=null, journalName=null, refType=null, unstructuredReference=ROEMISCH J, GRAY E, HOFFMANN JN,
et al. Antithrombin: a new look at the actions of a serine protease inhibitor[J].
Blood Coagul Fibrinolysis, 2002, 13(8): 657-670., articleTitle=null, refAbstract=null), Reference(id=1322626710676292519, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=null, journalName=null, refType=null, unstructuredReference=MARGETIC S, ELAP I, DELI BRKLJAI D,
et al. Chromogenic anti-FXa assay calibrated with low molecular weight heparin in patients treated with rivaroxaban and apixaban: possibilities and limitations[J].
Biochem Med (
Zagreb), 2020, 30(1): 010702., articleTitle=null, refAbstract=null), Reference(id=1322626710776955816, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=null, journalName=null, refType=null, unstructuredReference=HARRIS LF, CASTRO-LÓPEZ V, HAMMADI N,
et al. Development of a fluorescent anti-factor Xa assay to monitor unfractionated and low molecular weight heparins[J].
Talanta, 2010, 81(4-5): 1725-1730., articleTitle=null, refAbstract=null), Reference(id=1322626710890202025, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=null, journalName=null, refType=null, unstructuredReference=GRAY E, WALKER AD, HEATH AB. A collaborative study to establish the 2nd International standard for antithrombin concentrate[J].
Thromb Haemost, 1999, 82: 46-50., articleTitle=null, refAbstract=null), Reference(id=1322626710974088106, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=null, journalName=null, refType=null, unstructuredReference=SHOJAEI F, HOMAEI A, TAHERIZADEH MR,
et al. Characterization of biosynthesized chitosan nanoparticles from Penaeus vannamei for the immobilization of P. vannamei protease: an eco-friendly nanobiocatalyst[J].
Int J Food Prop, 2017, 20(Suppl 2): S1413-S1423., articleTitle=null, refAbstract=null), Reference(id=1322626711049585579, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[9], rfOrder=8, authorNames=null, journalName=null, refType=null, unstructuredReference=李京, 范慧红. 微量生色底物法测定肝素钠抗Xa因子和抗Ⅱa因子活性[J]. 中国生化药物杂志, 2010, 30(1): 45-47., articleTitle=null, refAbstract=null), Reference(id=1322626711129277356, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[10], rfOrder=9, authorNames=null, journalName=null, refType=null, unstructuredReference=CASTRO-LÓPEZ V, HARRIS LF, O'DONNELL JS,
et al. Comparative study of factor Xa fluorogenic substrates and their influence on the quantification of LMWHs[J].
Anal Bioanal Chem, 2011, 399(2): 691-700., articleTitle=null, refAbstract=null), Reference(id=1322626711234134957, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[11], rfOrder=10, authorNames=null, journalName=null, refType=null, unstructuredReference=齐元麟, 陈兵, 刘广芬, 等. 蕲蛇酶、凝血酶对人及牛纤维蛋白原的凝血反应比较[J]. 蛇志, 2000, 12(2): 50-53., articleTitle=null, refAbstract=null)], funds=[Fund(id=1322626710223307682, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, awardId=2012A5, language=CN, fundingSource=中国食品药品检定研究院中青年发展研究资助项目(2012A5), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1322626705546658682, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, xref=null, ext=[AuthorCompanyExt(id=1322626705563435899, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, companyId=1322626705546658682, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=National Institute for Food & Drug Control, Beijing 102629, China), AuthorCompanyExt(id=1322626705571824508, tenantId=1146029695717560320, journalId=1235980733773295621, articleId=1322626703386592119, companyId=1322626705546658682, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=中国食品药品检定研究院，北京 102629)])], figs=null, attaches=null, journal=Journal(id=1235980159023624194, delFlag=0, nameCn=中国新药杂志, nameEn=Chinese Journal of New Drugs, nameHistory1=null, nameHistory2=null, issn=1003-3734, eissn=null, cn=11-2850/R, coden=ZXZHA6, periodic=3, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=ohPEEN//Vz1tDT3ZBNTp4Q==, journalPrice=null, startedYear=null, abbrevIsoEn=Chinese Journal of New Drugs, journalRemark=null, publicationField=null, createdTime=1772610233314, updatedTime=1788948968376, createdBy=18614031015, updatedBy=13041195026, firstLetterCn=Z, firstLetterEn=Z, subjectCode=Medical and Pharmaceutical Sciences, subjectName=null, subjectCodeEn=Medical and Pharmaceutical Sciences, subjectNameEn=null, picCn=ohPEEN//Vz1tDT3ZBNTp4Q==, picEn=f9WK9bvTM4guTmS/iGhOMg==, jcr=null, cjcr=null, exts=[JournalExt(id=1304509780974924039, language=CN, name=中国新药杂志, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788948968404, updatedTime=1788948968404, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=http://draft.newdrug.cn/journalx_zgxy/authorLogOn.action, submissionEditorUrl=http://draft.newdrug.cn/journalx_zgxy/editorLogOn.action, submissionReviewUrl=http://draft.newdrug.cn/journalx_zgxy/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1304509781025255688, language=EN, name=Chinese Journal of New Drugs, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788948968416, updatedTime=1788948968416, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=http://draft.newdrug.cn/journalx_zgxy/authorLogOn.action, submissionEditorUrl=http://draft.newdrug.cn/journalx_zgxy/editorLogOn.action, submissionReviewUrl=http://draft.newdrug.cn/journalx_zgxy/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1235980733773295621, websiteList=[Website(id=1235982099191222761, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1235980733773295621, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgxyzz/CN, language=CN, createTime=1772610695884, createBy=18614031015, updateTime=1772610860397, updateBy=18614031015, name=中国新药杂志-中文, tplId=1146099689490845704, title=中国新药杂志, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1235983918629966444, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=articleTextType, value=kx, createTime=1772611129672, updateTime=1772611129672, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918604800617, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=banner, value=null, createTime=1772611129666, updateTime=1772611129666, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918655132271, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=grayFlag, value=0, createTime=1772611129678, updateTime=1772611129678, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918596412008, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=logo, value=https://castjournals.cast.org.cn/joweb/zgxyzz/CN/file/pic?fileId=W567vm71kFC7UhK9TSU6xw==, createTime=1772611129664, updateTime=1772611129664, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918667715185, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=minRunFlag, value=0, createTime=1772611129681, updateTime=1772611129681, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918617383531, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgxyzz/CN/file/pic, createTime=1772611129669, updateTime=1772611129669, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918659326576, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=silenceFlag, value=0, createTime=1772611129679, updateTime=1772611129679, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918613189226, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1772611129668, updateTime=1772611129668, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918634160749, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=themeColor, value=null, createTime=1772611129673, updateTime=1772611129673, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983918646743662, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099191222761, code=themeStyle, value=null, createTime=1772611129676, updateTime=1772611129676, creator=18614031015, updator=18614031015)]), Website(id=1235982099270914542, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1235980733773295621, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgxyzz/EN, language=EN, createTime=1772610695903, createBy=18614031015, updateTime=1772610875891, updateBy=18614031015, name=中国新药杂志-英文, tplId=1146101810881728533, title=Chinese Journal of New Drugs, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1235983955829248630, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=articleTextType, value=kx, createTime=1772611138541, updateTime=1772611138541, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955804082803, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=banner, value=null, createTime=1772611138535, updateTime=1772611138535, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955854414457, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=grayFlag, value=0, createTime=1772611138547, updateTime=1772611138547, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955795694194, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=logo, value=https://castjournals.cast.org.cn/joweb/zgxyzz/EN/file/pic?fileId=W567vm71kFC7UhK9TSU6xw==, createTime=1772611138533, updateTime=1772611138533, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955866997371, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=minRunFlag, value=0, createTime=1772611138550, updateTime=1772611138550, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955820860021, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgxyzz/EN/file/pic, createTime=1772611138539, updateTime=1772611138539, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955862803066, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=silenceFlag, value=0, createTime=1772611138549, updateTime=1772611138549, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955812471412, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1772611138537, updateTime=1772611138537, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955837637239, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=themeColor, value=null, createTime=1772611138543, updateTime=1772611138543, creator=18614031015, updator=18614031015), WebsiteProps(id=1235983955846025848, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1235982099270914542, code=themeStyle, value=null, createTime=1772611138545, updateTime=1772611138545, creator=18614031015, updator=18614031015)])], journalTitle=中国新药杂志, weixinUrl=null, journalUrl=http://www.newdrug.cn/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Chinese Journal of New Drugs, journalPhotoCn=ohPEEN//Vz1tDT3ZBNTp4Q==, journalPhotoEn=f9WK9bvTM4guTmS/iGhOMg==, journalFirstLetter=Z, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/zgxyzz/CN/Y2021/V30/I1/73, detailUrlEn=https://castjournals.cast.org.cn/joweb/zgxyzz/EN/Y2021/V30/I1/73, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zgxyzz/CN/PDF/Y2021/V30/I1/73, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zgxyzz/EN/PDF/Y2021/V30/I1/73, aliStartDate=0, aliEndDate=0, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1610640000000, fullTextJson=null, articleText=null, reference=null)
全屏
文章导航
PDF下载
12
3
关闭全屏
北京市海淀区学院南路86号
010-62662699（运营及技术支持）
qkjq@cast.org.cn
