中国新药杂志

标准桃金娘油对慢性阻塞性肺疾病大鼠Nrf2/HO-1通路的影响

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林琦 ¹^,²^,³ , 李紫如 ² , 郭锦玲 ² , 严晓萍 ² , 郑伟男 ³ , 林群英 ⁴

中国新药杂志 | 实验研究 2021,30(22): 2105-2110

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中国新药杂志 |实验研究 2021 , 30 (22) : 2105 -2110

标准桃金娘油对慢性阻塞性肺疾病大鼠Nrf2/HO-1通路的影响

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林琦¹^,²^,³, 李紫如², 郭锦玲², 严晓萍², 郑伟男³, 林群英⁴

作者信息

¹莆田学院附属医院药学部，莆田　351100

²莆田学院药学与医学技术学院，莆田　351100

³肿瘤转化医学福建省高校重点实验室，莆田　351100

⁴莆田学院附属医院呼吸与危重症医学科，莆田　351100

通讯作者:

林群英，女，二级教授，主任医师，主要从事呼吸病学的诊治与研究。联系电话：（0594）2292660，E-mail：linqyin@126.com。

作者简介:

林琦，男，副主任医师，副教授，医学博士，主要从事药物毒理学、药物动力学等临床药学研究。E-mail：linqitc@hotmail.com。

Study on the effect of standardized myrtle on Nrf2/HO-1 pathway in chronic obstructive pulmonary disease rats

Qi LIN¹^,²^,³, Zi-ru LI², Jin-ling GOU², Xiao-ping YAN², Wei-nan ZHENG³, Qun-ying LIN⁴

Affiliations

¹Department of Pharmacy, The Affiliated Hospital of Putian University, Putian 351100, China

²School of Pharmacy an Medical Technology, Putian University, Putian 351100, China

³Key Laboratory of Translational Tumor Medicine in Fujian Province, Putian University, Putian 351100, China

⁴Department of Pulmonary and Critical Care Medicine, The Affiliated Hospital of Putian University, Putian 351100, China

出版时间: 2021-11-30

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摘要

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目的：

观察标准桃金娘油（myrtol standardized，MS）对慢性阻塞性肺疾病（COPD）大鼠核因子E2相关因子2（Nrf2）/血红素加氧酶1（HO-1）通路的影响，探讨标准桃金娘油治疗COPD可能的作用机制。

方法：

将Wistar大鼠随机分为4组，1组为空白对照组（CON），另外3组首先使用香烟（CS）慢性暴露90 d建立COPD模型，1组给予持续CS暴露（CSM）、1组停止CS暴露（QSM）、1组停止CS暴露并给予MS处理（MSM），持续30 d。造模结束后，分别检测大鼠呼吸功能、血清炎症因子[白介素6（IL6）和肿瘤坏死因子-α（TNF-α）]、肺组织抗氧化应激指标[总超氧化物歧化酶（T-SOD）和丙二醛（MDA）]、肺组织HE染色观察、蛋白质印迹（WB）检测肺组织Nrf2和HO-1蛋白表达。

结果：

CSM组呼吸功能明显减退（P<0.05），QSM和MSM组呼吸功能有所改善（P<0.05）；MSM组的IL6和TNF-α显著低于CSM组（P<0.05），T-SOD显著升高（P<0.05），MDA显著下降（P<0.05）；HE染色可见CSM组出现肺气肿样改变，MSM组可见肺组织结构异常的改善；同时MSM组Nrf2和HO-1蛋白表达相对于CSM和QSM组升高（P<0.05），接近于CON组（P>0.05）。

结论：

MS可显著改善COPD大鼠的呼吸功能、减少炎症浸润、减弱氧化应激，其内在机制与Nrf2/HO-1通路激活密切相关。

关键词

慢性阻塞性肺疾病 / 标准桃金娘油 / 核因子E2相关因子2 / 血红素加氧酶1 / 氧化应激

Abstract

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Objective:

To observe the influence of myrtol standardized (MS) on the Nrf2/HO-1 pathway in chronic obstructive pulmonary disease (COPD) rats, and explore the possible mechanism of MS in treating COPD.

Methods:

Wistar rats were randomly divided into four groups: one was control group (CON), while the other three groups were exposed to cigarettes (CS) for 90 d to establish a COPD model firstly, then one group was given CS exposure (CSM) continuously, one group was quitted CS exposure (QSM), the other one group was quitted CS exposure and then given MS treatment (MSM), all the operations lasted for 30 d. After the modeling, the respiratory function, serum inflammatory factors (IL6, TNF-α), and lung tissue anti-oxidative stress indicators (T-SOD, MDA) were detected, HE staining of lung tissue was observed, and the expressions of Nrf2 and HO-1 in lung tissue by WB were analyzed.

Results:

The respiratory function of the CSM group decreased significantly (P<0.05), and the respiratory function of the QSM and MSM groups improved (P<0.05); the IL6 and TNF-α levels of the MSM group were significantly lower than those of the CSM group (P<0.05), and the T-SOD level increased (P<0.05), MDA decreased (P<0.05); HE staining showed emphysema-like forms in the lung tissues of the CSM group, MSM group could improve the abnormal structure of lung tissue. Meanwhile, the expressions of Nrf2 and HO-1 protein in the MSM group were relatively increased than the CSM and QSM groups (P<0.05), which was close to the CON group (P>0.05).

Conclusion:

MS could significantly improve the respiratory function of COPD rats, reduce inflammation and infiltration, and weaken oxidative stress. Its internal mechanism is closely related to the activation of the Nrf2/HO-1 pathway.

Key words

chronic obstructive pulmonary disease / myrtol standardized / nuclear factor erythroid 2 related factor 2 / heme oxygenase 1 / oxidative stress

引用本文

林琦, 李紫如, 郭锦玲, 严晓萍, 郑伟男, 林群英. 标准桃金娘油对慢性阻塞性肺疾病大鼠Nrf2/HO-1通路的影响. 中国新药杂志, 2021 , 30 (22) : 2105 -2110 .

Qi LIN, Zi-ru LI, Jin-ling GOU, Xiao-ping YAN, Wei-nan ZHENG, Qun-ying LIN. Study on the effect of standardized myrtle on Nrf2/HO-1 pathway in chronic obstructive pulmonary disease rats[J]. Chinese Journal of New Drugs, 2021 , 30 (22) : 2105 -2110 .

基金

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福建省自然科学基金面上项目(2020J011251)
福建省卫生健康科技计划资助项目(2020GGA078)
福建省教育厅中青年教师教育科研立项资助项目(JAT190563)
莆田市科技计划资助项目(2019S3F002)
莆田学院校级教育教学改革研究立项项目(JG202047)
福建省大学生创新训练资助项目(S202111498007)

参考文献

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文献

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参考文献引证文献

排序方式：

[1]

FANG L, GAO P, BAO H, et al. Chronic obstructive pulmonary disease in China: a nationwide prevalence study[J]. Lancet Respir Med, 2018, 6(6): 421-430.

[2]

WANG C, XU J, YANG L, et al. Prevalence and risk factors of chronic obstructive pulmonary disease in China (the China Pulmonary Health [CPH] study): a national cross-sectional study[J]. Lancet, 2018, 391(10131): 1706-1717.

[3]

CANTOR JO, TURINO GM. COPD pathogenesis: finding the common in the complex[J]. Chest, 2019, 155(2): 266-271.

[4]

YUAN C, CHANG, LU G, et al. Genetic polymorphism and chronic obstructive pulmonary disease[J]. Int J Chron Obstruct Pulmon Dis, 2017, 12: 1385-1393.

[5]

刘立立, 陈路佳, 占美, 等. 标准桃金娘油治疗慢性支气管炎和COPD的系统评价[J]. 中国药房, 2012, 23(16): 1496-1500.

[6]

杜宁, 刘小强, 段争. 标准桃金娘油减轻PM2.5致大鼠气道黏液纤毛的损伤[J]. 基础医学与临床, 2020, 40(3): 356-362.

[7]

RANTZSCH U, VACCA G, DÜCK R, et al. Anti-inflammatory effects of myrtol standardized and other essential oils on alveolar macrophages from patients with chronic obstructive pulmonary disease[J]. Eur J Med Res, 2009, 14(Suppl 4): S205-S209.

[8]

周建娅, 唐法娣, 王砚, 等. 二氧化硫吸入致大鼠肺组织核因子κB的激活及蓝桉油的抑制作用[J]. 中华结核和呼吸杂志, 2003, 26(5): 311-312.

[9]

GRAßMANNA J, HIPPELIA S, DORNISCHA K, et al. Antioxidant properties of essential oils: possible explanations for their anti-inflammatory effects[J]. Arzneim Forsch, 2000, 50(2): 135-139.

[10]

MOI P, CHAN K, ASUNIS I, et al. Isolation of NF-E2-related factor 2 (Nrf2), a NF-E2-like basic leucine zipper transcriptional activator that binds to the tandem NF-E2/AP1 repeat of the beta-globin locus control region[J]. PNAS, 1994, 91(21): 9926-9930.

[11]

FRATTA PASINI AM, STRANIERI C, FERRARI M, et al. Oxidative stress and Nrf2 expression in peripheral blood mononuclear cells derived from COPD patients: an observational longitudinal study[J]. Respir Res, 2020, 21(1): 37-48.

[12]

JONES B, DONOVAN C, LIU G, et al. Animal models of COPD: what do they tell us?[J]. Respirology, 2017, 22(1): 21-32.

[13]

WRIGHT JL, CHURG A. Animal models of cigarette smoke-induced COPD[J]. Chest, 2002, 122(6): S301-S306.

[14]

KIRKHAM PA, BARNES PJ. Oxidative stress in COPD[J]. Chest, 2013, 144(1): 266-273.

[15]

BOUKHENOUNA S, WILSON MA, BAHMED K, et al. Reactive oxygen species in chronic obstructive pulmonary disease[J]. Oxid Med Cell Longev, 2018, 2018: 5730395.

[16]

ZUO L, WIJEGUNAWARDANA D. Redox role of ROS and inflammation in pulmonary diseases[J]. Adv Exp Med Biol, 2021, 1304: 187-204.

[17]

BERNARDO I, BOZINOVSKI S, VLAHOS R. Targeting oxidant-dependent mechanisms for the treatment of COPD and its comorbidities[J]. Pharmacol Ther, 2015, 155: 60-79.

[18]

DANG X, HE B, NING Q, et al. Alantolactone suppresses inflammation, apoptosis and oxidative stress in cigarette smoke-induced human bronchial epithelial cells through activation of Nrf2/HO-1 and inhibition of the NF-κB pathways[J]. Respir Res, 2020, 21(1): 95.

[19]

ESPINOSA-DIEZ C, MIGUEL V, MENNERICH D, et al. Antioxidant responses and cellular adjustments to oxidative stress[J]. Redox Biol, 2015, 6: 183-197.

[20]

曾梦莹, 马晓丽. 基于Keap1-Nrf2/ARE信号通路研究牛磺酸对胰岛素抵抗模型大鼠氧化应激的影响[J]. 中国现代应用药学, 2020, 37(22): 2703-2707.

[21]

崔磊. 平喘养肺汤联合布地奈德治疗COPD稳定期患者的疗效与安全性研究[J]. 世界中医药, 2020, 15(14): 2097-2100.

[22]

谭亲友, 刘兵, 张靖, 等. 基于Nrf2信号通路探讨异甘草酸镁对雷公藤甲素损伤L-02细胞中相关蛋白表达的影响[J]. 中国现代应用药学, 2020, 37(2): 138-142.

[23]

张永华, 谷伟, 毛山, 等. 慢性阻塞性肺疾病稳定期治疗药物的优化选择[J]. 世界临床药物, 2021, 42(8): 675-679.

2021年第30卷第22期

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首发时间：2026-10-28
出版时间：2021-11-30

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出版历史

录用日期：2021-08-22

基金

福建省自然科学基金面上项目(2020J011251)

福建省卫生健康科技计划资助项目(2020GGA078)

福建省教育厅中青年教师教育科研立项资助项目(JAT190563)

莆田市科技计划资助项目(2019S3F002)

莆田学院校级教育教学改革研究立项项目(JG202047)

福建省大学生创新训练资助项目(S202111498007)

作者信息

¹莆田学院附属医院药学部，莆田　351100

²莆田学院药学与医学技术学院，莆田　351100

³肿瘤转化医学福建省高校重点实验室，莆田　351100

⁴莆田学院附属医院呼吸与危重症医学科，莆田　351100

通讯作者:

林群英，女，二级教授，主任医师，主要从事呼吸病学的诊治与研究。联系电话：（0594）2292660，E-mail：linqyin@126.com。

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https://castjournals.cast.org.cn/joweb/zgxyzz/CN/1322125586802258226

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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