中国新药杂志

基于c-JNK/CXCL1信号通路研究杜仲腰痛丸对腰椎间盘突出症大鼠脊髓背角组织炎症的抑制作用

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刘俊豪 ¹ , 赵继荣 ¹^,²^,^* , 湛洋 ¹ , 殷红年 ¹ , 蒋鹏 ² , 王化省 ¹ , 马东 ¹ , 赵瑞 ¹ , 马伯骞 ¹

中国新药杂志 | 实验研究 2026,35(13): 1417-1425

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中国新药杂志 |实验研究 2026 , 35 (13) : 1417 -1425

基于c-JNK/CXCL1信号通路研究杜仲腰痛丸对腰椎间盘突出症大鼠脊髓背角组织炎症的抑制作用

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刘俊豪¹, 赵继荣¹^,²^,^*, 湛洋¹, 殷红年¹, 蒋鹏², 王化省¹, 马东¹, 赵瑞¹, 马伯骞¹

作者信息

¹甘肃中医药大学，兰州 730000

²甘肃中医药大学附属医院，兰州 730000

通讯作者:

^* 赵继荣，男，一级主任医师，博士生导师，研究方向：中医药防治脊柱退行性疾患的临床与基础研究。E-mail: 359973018@qq.com。

作者简介:

刘俊豪，男，硕士研究生，研究方向：中医药防治脊柱退行性疾患的临床与基础研究。E-mail: 1596695819@qq.com。

Inhibitory effect of Duzhong Yaotong Pill on inflammation of spinal dorsal horn in rats with lumbar discs herniation based on c-JNK/CXCL1 signal pathway

Jun-hao LIU¹, Ji-rong ZHAO¹^,²^,^*, Yang ZHAN¹, Hong-nian YIN¹, Peng JIANG², Hua-sheng WANG¹, Dong MA¹, Rui ZHAO¹, Bo-qian MA¹

Affiliations

¹Gansu University of Chinese Medicine, Lanzhou 730000, China

²Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou 730000, China

出版时间: 2026-07-15 doi: 10.20251/j.cnki.1003-3734.2026.13.011

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摘要

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目的：

分析杜仲腰痛丸调节c-Jun氨基末端激酶（c-Jun N-terminal kinase, c-JNK）/趋化因子配体1[chemokine（C-X-C motif）ligand 1, CXCL1]信号通路对腰椎间盘突出症（lumbar intervertebral disc protrusion, LDH）大鼠脊髓背角组织炎症的影响。

方法：

将75只大鼠随机分为假手术组，模型组，杜仲腰痛丸低、中、高剂量组。模型复制2 h后，杜仲腰痛丸剂量组灌胃给予杜仲腰痛丸溶液，假手术组和模型组灌胃给予生理盐水。干预后观察各组大鼠一般情况，痛觉过敏实验测定机械刺激缩足反射阈值（paw withdrawal mechanical threshold, PWMT）和热刺激缩足反射潜伏期（paw withdrawal thermal latency, PWTL），ELISA检测大鼠脊髓背角组织白细胞介素6（interleukin-6, IL-6）和IL-1β水平，HE染色法观察大鼠背根神经节组织病理学变化，TUNEL染色检测脊髓细胞凋亡情况，qRT-PCR检测离子钙结合接头分子1（ion calcium binding junction molecule 1, Iba-1）、c-JNK、p-c-JNK、CXCL1mRNA表达，Westernblot检测Iba-1、c-JNK、p-c-JNK、CXCL1通路蛋白表达。

结果：

与假手术组相比，模型组大鼠神经细胞萎缩，细胞核和尼氏小体减少，细胞凋亡率明显升高，IL-6、IL-β、Iba-1、p-c-JNK、CXCL1mRNA表达及蛋白表达升高，PWMT、PWTL下降（P<0.05）；与模型组相比，杜仲腰痛丸低、中、高剂量组神经细胞形态恢复，核仁清晰，尼氏小体增加，细胞凋亡率明显下降，IL-6、IL-β、Iba-1、p-c-JNK、CXCL1mRNA表达及蛋白表达下降，PWMT、PWTL升高（P<0.05）。c-JNK的mRNA表达及蛋白表达相对表达水平组间比较差异无统计学意义（P>0.05）。

结论：

杜仲腰痛丸能够抑制LDH大鼠脊髓背角组织炎症反应，作用机制可能与抑制c-JNK/CXCL1信号通路有关。

关键词

腰椎间盘突出症 / 杜仲腰痛丸 / 脊髓炎症 / c-JNK/CXCL1信号通路

Abstract

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Objective:

To investigate the effect of Duzhong Yaotong Pill on spinal dorsal horn inflammation in rats with lumbar disc herniation (LDH) by regulating the c-Jun N-terminal kinase (c-JNK)/chemokine (C-X-C motif) ligand 1 (CXCL1) signaling pathway.

Methods:

Seventy-five rats were randomly divided into a sham operation group, a model group, and low-, medium-, and high-dose Duzhong Yaotong Pill groups. Two hours after model establishment, the Duzhong Yaotong Pill groups were gavaged with Duzhong Yaotong Pill solution, while the sham operation group and the model group were gavaged with normal saline. The general condition of the rats in each group was observed. The paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured by hyperalgesia testing. The levels of interleukin-6 (IL-6) and interleukin-1β (IL-1β) in the spinal dorsal horn tissue were detected by ELISA. Histopathological changes in the dorsal root ganglion were observed by HE staining. Spinal cord cell apoptosis was detected by TUNEL staining. The mRNA expression of ionized calcium-binding adapter molecule 1 (Iba-1), c-JNK, p-c-JNK and CXCL1 was detected by qRT-PCR, and the protein expression of Iba-1, c-JNk, p-d-JNK and CXCL1 was detected by Westernblot.

Results:

Compared with the sham operation group, the model group showed neuronal atrophy, reduced nuclei and Nissl bodies, a significantly increased apoptosis rate, increased levels of IL-6, IL-β, Iba-1, p-c-JNK, and CXCL1, and decreased PWMT and PWTL (all P<0.05). Compared with the model group, the low-, medium-, and high-dose Duzhong Yaotong Pill groups showed recovery of neuronal morphology, distinct nucleoli, increased Nissl bodies, a significantly decreased apoptosis rate, decreased levels of IL-6, IL-β, decreased mRNA expression and protein expression of Iba-1, p-c-JNK, and CXCL1, and increased PWMT and PWTL (all P<0.05). There were no significant differences in the mRNA and protein expression levels of c-JNK among groups (P>0.05).

Conclusion:

Duzhong Yaotong Pill can inhibit spinal dorsal horn inflammation in LDH rats, and its mechanism may be related to the inhibition of the c-JNK/CXCL1 signaling pathway.

Key words

lumbar disc herniation / Duzhong Yaotong Pill / spinal cord inflammation / c-Jun N-terminal kinase/CXCL1 signal pathway

引用本文

刘俊豪, 赵继荣, 湛洋, 殷红年, 蒋鹏, 王化省, 马东, 赵瑞, 马伯骞. 基于c-JNK/CXCL1信号通路研究杜仲腰痛丸对腰椎间盘突出症大鼠脊髓背角组织炎症的抑制作用. 中国新药杂志, 2026 , 35 (13) : 1417 -1425 . DOI: 10.20251/j.cnki.1003-3734.2026.13.011

Jun-hao LIU, Ji-rong ZHAO, Yang ZHAN, Hong-nian YIN, Peng JIANG, Hua-sheng WANG, Dong MA, Rui ZHAO, Bo-qian MA. Inhibitory effect of Duzhong Yaotong Pill on inflammation of spinal dorsal horn in rats with lumbar discs herniation based on c-JNK/CXCL1 signal pathway[J]. Chinese Journal of New Drugs, 2026 , 35 (13) : 1417 -1425 . DOI: 10.20251/j.cnki.1003-3734.2026.13.011

基金

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国家自然科学基金资助项目(82460940)
兰州市科技计划资助项目(2023-2-60)
甘肃省自然科学基金资助项目(23JRRA1248)

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2026年第35卷第13期

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文章信息

doi: 10.20251/j.cnki.1003-3734.2026.13.011

首发时间：2026-10-26
出版时间：2026-07-15

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出版历史

录用日期：2025-10-30

基金

国家自然科学基金资助项目(82460940)

兰州市科技计划资助项目(2023-2-60)

甘肃省自然科学基金资助项目(23JRRA1248)

作者信息

¹甘肃中医药大学，兰州 730000

²甘肃中医药大学附属医院，兰州 730000

通讯作者:

^* 赵继荣，男，一级主任医师，博士生导师，研究方向：中医药防治脊柱退行性疾患的临床与基础研究。E-mail: 359973018@qq.com。

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https://castjournals.cast.org.cn/joweb/zgxyzz/CN/10.20251/j.cnki.1003-3734.2026.13.011

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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