Article(id=1321425575609221808, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1321425569611362746, articleNumber=null, orderNo=null, doi=10.20251/j.cnki.1003-3734.2026.07.009, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=1750953600000, acceptedDateStr=2025-06-27, onlineDate=1792982008124, onlineDateStr=2026-10-26, pubDate=1776182400000, pubDateStr=2026-04-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1792982008124, onlineIssueDateStr=2026-10-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1792982008124, creator=13701087609, updateTime=1792982008124, updator=13701087609, issue=Issue{id=1321425569611362746, tenantId=1146029695717560320, journalId=1235980733773295621, year='2026', volume='35', issue='7', pageStart='673', pageEnd='784', issueExtLink='null', onlineDate='null', pubDate='1776182400000', pubDateStr='2026-04-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1792982006695, creator='13701087609', updateTime=1792983136833, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1321430309837587328, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1321425569611362746, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1321430309837587329, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1321425569611362746, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=734, endPage=740, ext={EN=ArticleExt(id=1321425576007680689, articleId=1321425575609221808, tenantId=1146029695717560320, journalId=1235980733773295621, language=EN, title=Simultaneous determination of 10 components in Puqin Jiedu Lishi granules by HPLC-PDA method, columnId=null, journalTitle=Chinese Journal of New Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=
Objective: To establish an HPLC-PDA method for the simultaneous determination of 5-hydroxymethylfurfural, chlorogenic acid, caffeic acid, isochlorogenic acid A, cichoric acid, baicalin, oroxylin A-7-O-β-D-glucuronide, wogonoside, baicalein and glycyrrhizic acid in the Puqin Jiedu Lishi Granules.
Methods: The analysis was performed on a Waters Symmetry C18 column (250 mm×4.6 mm, 5 μm) with gradient elution using acetonitrile and 0.2% phosphoric acid solution as the mobile phase. The flow rate was 1.0 mL·min-1, the column temperature was 20 ℃, and the detection wavelengths were set at 284 nm, 327 nm, 280 nm and 254 nm.
Results: The linear ranges of 5-hydroxymethylfurfural, chlorogenic acid, caffeic acid, isochlorogenic acid A, cichoric acid, baicalin, oroxylin A-7-O-β-D-glucuronide, wogonoside, baicalein and glycyrrhizic acid were 1.860~59.64, 5.425~173.6, 1.980~63.36, 1.890~60.24, 5.990~191.6, 33.30~1066, 6.440~206.0, 28.00~896.0, 2.044~65.40, 6.660~213.1 μg·mL-1, respectively, with the correlation coefficients r≥0.999 5. The precision, stability, and reproducibility tests yielded satisfactory results. The average recoveries of the 10 components ranged from 98.15%~102.21%, with RSDs between 0.96%~1.91%.
Conclusion: This method is reliable, stable and reproducible, providing a reference for the quality control of Puqin Jiedu Lishi Granules.
, authors=Shuai-yin LI
1, Hui-rong XI
2, Ting CHEN
1, Xi-xiang LI
2, *, Xiao-feng LI
1, Ya-li ZHOU
1, Shou-yuan YANG
3, authorsList=Shuai-yin LI, Hui-rong XI, Ting CHEN, Xi-xiang LI, Xiao-feng LI, Ya-li ZHOU, Shou-yuan YANG, authorCompany=null, correspAuthors=Xi-xiang LI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1321425576255144626, articleId=1321425575609221808, tenantId=1146029695717560320, journalId=1235980733773295621, language=CN, title=HPLC-PDA法同时测定蒲芩解毒利湿颗粒中10种成分的含量, columnId=1236330556003242277, journalTitle=中国新药杂志, columnName=实验研究, runingTitle=null, highlight=null, articleAbstract=
目的: 建立HPLC-PDA法同时测定蒲芩解毒利湿颗粒中5-羟甲基糠醛、绿原酸、咖啡酸、异绿原酸A、菊苣酸、黄芩苷、千层纸素A-7-O-β-D-葡糖醛酸苷、汉黄芩苷、黄芩素、甘草酸的含量。
方法: 采用Waters Symmetry C18色谱柱(250 mm×4.6 mm, 5 μm),以乙腈-0.2%磷酸溶液为流动相,梯度洗脱;流速1.0 mL·min-1;柱温20 ℃;检测波长284、327、280、254 nm。
结果: 5-羟甲基糠醛、绿原酸、咖啡酸、异绿原酸A、菊苣酸、黄芩苷、千层纸素A-7-O-β-D-葡糖醛酸苷、汉黄芩苷、黄芩素、甘草酸的线性范围分别为1.860~59.64、5.425~173.6、1.980~63.36、1.890~60.24、5.990~191.6、33.30~1 066、6.440~206.0、28.00~896.0、2.044~65.40、6.660~213.1 μg·mL-1,相关系数r均≥0.999 5。精密度、稳定性和重复性试验良好。10种组分的平均加样回收率在98.15%~102.21%,RSD在0.96%~1.91%。
结论: 该方法可靠稳定且重复性好,可为蒲芩解毒利湿颗粒的质量控制提供参考。
, authors=李帅印
1, 葸慧荣
2, 陈挺
1, 李喜香
2, *, 李小凤
1, 周亚丽
1, 杨寿圆
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