Article(id=1236700466738950797, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1236700465119949418, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=1666972800000, acceptedDateStr=2022-10-29, onlineDate=1772781968054, onlineDateStr=2026-03-06, pubDate=1681488000000, pubDateStr=2023-04-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772781968054, onlineIssueDateStr=2026-03-06, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772781968054, creator=13701087609, updateTime=1772781968054, updator=13701087609, issue=Issue{id=1236700465119949418, tenantId=1146029695717560320, journalId=1235980733773295621, year='2023', volume='32', issue='7', pageStart='657', pageEnd='760', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772781967668, creator=13701087609, updateTime=1772782901622, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236704382474047808, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1236700465119949418, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236704382474047809, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1236700465119949418, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=747, endPage=751, ext={EN=ArticleExt(id=1236700467003191963, articleId=1236700466738950797, tenantId=1146029695717560320, journalId=1235980733773295621, language=EN, title=Qualitative and quantitative analysis of vemurafenib by nuclear magnetic resonance spectroscopy, columnId=null, journalTitle=Chinese Journal of New Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=

Objective: To establish a quantitative proton nuclear magnetic resonance method for the determination of vemurafenib.Methods:The structure of vemurafenib was confirmed by MS and NMR. The spectra of proton nuclear magnetic resonance spectroscopy were obtained in dimethylsulfoxide-d₆ by using Bruker ANANCE NEO 600, the parameters of which were set up as follows: 90° pulse sequences, the relaxation delay time was 15 s, the number for scanning was 32, the experiment temperature was controlled at 25 ℃, and hydroquinone were selected as internal standard. The quantitative study was conducted on vemurafenib.Results:The ¹H and ¹³C NMR spectra of vemurafenib was assigned. The content of vemurafenib determined by qHNMR were 98.78%, which was basically consistent with the results of mass balance method (98.91%).Conclusion:The method is simple, rapid and accurate, and can be used for the determination of vemurafenib.

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目的：建立定量核磁共振氢谱法（qHNMR）测定维莫非尼含量的方法。方法：首先采用质谱、核磁共振方法对维莫非尼结构进行确证，然后采用Bruker ANANCE NEO 600型核磁共振波谱仪测定一维定量氢谱，90°脉冲，弛豫时间（D1）15 s，扫描次数（NS）32次，测定温度25 ℃，以对苯二酚为内标，氘代二甲基亚砜（DMSO-d₆）为溶剂，对维莫非尼进行定量研究。结果：对维莫非尼核磁共振氢谱和碳谱数据进行归属，利用qHNMR对维莫非尼进行定量分析，测得含量为98.78%，与质量平衡法测得的结果（98.91%）基本一致。结论：建立的qHNMR法操作简单、快速、准确，可用于维莫非尼含量测定。

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陈忠兰，女，药师，研究方向：药品标准物质研究。E-mail: 550736995@qq.com。

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陈忠兰，女，药师，研究方向：药品标准物质研究。E-mail: 550736995@qq.com。

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