Article(id=1236696516199510059, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1236696515473895464, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=1655568000000, acceptedDateStr=2022-06-19, onlineDate=1772781026172, onlineDateStr=2026-03-06, pubDate=1673712000000, pubDateStr=2023-01-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772781026172, onlineIssueDateStr=2026-03-06, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772781026172, creator=13701087609, updateTime=1772781026172, updator=13701087609, issue=Issue{id=1236696515473895464, tenantId=1146029695717560320, journalId=1235980733773295621, year='2023', volume='32', issue='1', pageStart='1', pageEnd='109', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772781025999, creator=13701087609, updateTime=1772782685740, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236703476982542786, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1236696515473895464, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236703476982542787, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1236696515473895464, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=86, endPage=92, ext={EN=ArticleExt(id=1236696516400836654, articleId=1236696516199510059, tenantId=1146029695717560320, journalId=1235980733773295621, language=EN, title=Simultaneous determination of eight components in Shensu Ganmao tablets using two-reference multi-component method, columnId=null, journalTitle=Chinese Journal of New Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=

Objective: To develop the determination method of the contents of 3′-hydroxy puerarin, puerarin, puerarin apioside, narirutin, naringin, hesperidin, neohesperidin, and praeruptorin A in Shensu Ganmao tablets (SGT) using high performance liquid chromatography with two-reference multi-component method (TRMC). Methods: The analyses were performed on an Agilent ZORBAX SB-C₁₈ column (250 mm×4.6 mm, 5 μm) using the mobile phase of 0.1% phosphoric acid-acetonitrile in gradient elution mode at a flow rate of 1.0 mL·min^-1. The detection wavelength was 283 nm. Results: Taking puerarin as internal reference, the RCF of 3′-hydroxy puerarin and puerarin apioside are 0.853 and 1.354. Taking naringin as internal reference, the relative correlation factors (RCF) of narirutin, hesperidin, neohesperidin, and praeruptorin A are 1.181, 1.027, 1.002, and 2.188. The RCF showed validated on different experimental conditions. The results of 8 components in SGT were in acceptable limits by using TRMC and the external standard method (ESM). Conclusion: The newly established TRMC can determine the characteristic constituents in Puerariae Lobatae Radix, Citri Reticulatae Pericarpium, Aurantii Fructus, and Peucedani Radix in SGT simultaneously, which can be used for quality control of SGT.

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目的：建立参苏感冒片中3′-羟基葛根素、葛根素、葛根素芹菜糖苷、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、白花前胡甲素的高效液相色谱双标多测的含量测定方法。方法：采用Agilent ZORBAX SB-C₁₈色谱柱（250 mm×4.6 mm，5 μm），以0.1%磷酸-乙腈为溶液梯度洗脱，流速：1.0 mL·min^-1，检测波长：283 nm。结果：以葛根素为内参物，参苏感冒片中的3′-羟基葛根素、葛根素芹菜糖苷的相对校正因子为0.853，1.354，以柚皮苷为内参物，芸香柚皮苷、橙皮苷、新橙皮苷、白花前胡甲素的相对校正因子为1.181，1.027，1.002，2.188，不同条件下耐用性良好，双标多测与外标一点法所测结果RSD均小于1%，方法可行。结论：建立了双标多测法同时测定参苏感冒片中葛根、陈皮、枳壳、前胡中特征性成分的含量，从而能更充分地控制参苏感冒片的整体质量。

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陈晶，女，主管药师，主要从事中药成分分析研究。E-mail: chenjing4477@163.com。

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陈晶，女，主管药师，主要从事中药成分分析研究。E-mail: chenjing4477@163.com。

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