Article(id=1236687126260151259, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1236687122619495346, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=1665936000000, acceptedDateStr=2022-10-17, onlineDate=1772778787436, onlineDateStr=2026-03-06, pubDate=1682784000000, pubDateStr=2023-04-30, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772778787436, onlineIssueDateStr=2026-03-06, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772778787436, creator=13701087609, updateTime=1772778787436, updator=13701087609, issue=Issue{id=1236687122619495346, tenantId=1146029695717560320, journalId=1235980733773295621, year='2023', volume='32', issue='8', pageStart='761', pageEnd='864', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772778786568, creator=13701087609, updateTime=1772779118928, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236688516713861515, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1236687122619495346, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236688516713861516, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1236687122619495346, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=853, endPage=857, ext={EN=ArticleExt(id=1236687128151782377, articleId=1236687126260151259, tenantId=1146029695717560320, journalId=1235980733773295621, language=EN, title=Analysis of monosaccharide residues composition in human erythropoietin, columnId=null, journalTitle=Chinese Journal of New Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=
Objective: To analyze the monosaccharide composition of human erythropoietin by cIEF-MS.
Methods: The cIEF peak was obtained after cIEF detection, and each peak was detected by MS to obtain the high resolution mass spectral molecular weight. After deducting the protein molecular weight, the redundant molecular weight was compared with the molecular weight combination database of monosaccharide residues (accuracy being about 0.01%) to obtain a series of monosaccharide combination manners with basically consistent mass, and the rationality of monosaccharide quantity and the relationship between monosaccharides were analyzed.
Results: Ten cIEF peaks were obtained by cIEF detection, and no less than 32 peak mass numbers were obtained by MS detection. After comparison, a series of monosaccharide combination forms corresponding to each mass number were obtained. Only the combination form with the highest ion number P3 was listed in this paper. The effectiveness analysis results showed that the most extensible combination manners were 19 GlcNAc/GlaNAc residues, 22 Man/Gla residues, 12 Neu5Ac residues and 3 Fuc residues.
Conclusion: The monosaccharide combination manner of human erythropoietin protein glycosylation chain was obtained, which laid a foundation for the further analysis and characterization of complete protein glycoforms.
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目的： 利用人促红素cIEF-MS检测结果，解析其糖链单糖组成。
方法： 经cIEF检测后获得cIEF峰，并对每个峰进行了MS检测，得到每个峰的高分辨质谱分子量，扣除蛋白分子量后，将分子量差值与单糖残基分子量组合数据库进行比对（精度约0.01%），获得一系列质量数基本相符的单糖组合方式，并对单糖数量及单糖之间关系合理性进行分析。
结果： 经cIEF检测后获得10个cIEF峰，MS检测获得不少于32个峰质量数，比较后获得各质量数对应的一系列单糖组合形式，文中只列举了离子数量最高峰P3的组合形式，经有效性分析结果显示其最具有延伸性的组合方式为19个GlcNAc/GlaNAc残基、22个Man/Gla残基、12个Neu5Ac残基和3个Fuc残基。
结论： 获得人促红素蛋白糖链单糖组合方式，为进一步完整蛋白糖型的解析和表征奠定基础。
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