Article(id=1236330555705446668, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1236330549866975405, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=1668614400000, acceptedDateStr=2022-11-17, onlineDate=1772693774391, onlineDateStr=2026-03-05, pubDate=1697299200000, pubDateStr=2023-10-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772693774391, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772693774391, creator=13701087609, updateTime=1772693774391, updator=13701087609, issue=Issue{id=1236330549866975405, tenantId=1146029695717560320, journalId=1235980733773295621, year='2023', volume='32', issue='19', pageStart='1905', pageEnd='2008', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772693772998, creator=13701087609, updateTime=1772695059296, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236335945046348400, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1236330549866975405, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236335945046348401, tenantId=1146029695717560320, journalId=1235980733773295621, issueId=1236330549866975405, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=2001, endPage=2008, ext={EN=ArticleExt(id=1236330555915161885, articleId=1236330555705446668, tenantId=1146029695717560320, journalId=1235980733773295621, language=EN, title=Simultaneous determination of 12 components in Ji-Ni De-Xie by HPLC-QqQ-MS, columnId=null, journalTitle=Chinese Journal of New Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=
Objective: To establish the method of simultaneous determination of 12 major chemical components (such as berberine, magnolflorine and curcumin) in Tibetan medicine Ji-Ni De-Xie.
Methods: The separation was achieved on a WondaSil C18-WR column. High performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-QqQ-MS) was used with the mobile phase 0.1% formic acid solution (A)-methanol (B) under gradient elution. The temperature of Electron Spray Ionization (ESI) was 300 ℃. The charged ions were scanned in positive and negative ion modes at the same time with multiple reaction monitoring. The contents of 12 components in 5 batches of samples were calculated according to the linear equation of 12 reference substances.
Results: The 12 components of the Ji-Ni De-Xie have a good linear relationship within the detection range. The average contents of magnolflorine, jatrorrhizine, berberine, curcumin, demethoxycurcumin, gallic acid, chebulagic acid, ferulic acid 4-O-β-D-glucopyranoside, hydroxysafflor yellow A, rutin, ferulic acid, and ellagic acid are 3.18, 0.20, 1.71, 0.52, 0.22, 15.93, 7.56, 3.29, 0.07, 0.20, 1.47, 2.89 mg·g-1, respectively.
Conclusion: The established analytical method is highly selective with high sensitivity. providing reference for the quality control of the Tibetan medicine Ji-Ni De-Xie.
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目的： 建立同时测定吉尼德协中小檗碱、木兰花碱、姜黄素等12种成分含量的方法。
方法： 以WondaSil C18-WR色谱柱，流动相为0.1%甲酸（A）-甲醇（B）进行梯度洗脱；采用高效液相色谱-三重四极杆质谱（HPLC-QqQ-MS）仪，电喷雾离子源（ESI）温度为300 ℃，带电离子在正、负离子模式同时扫描，多重反应监测模式下检测；依据12种对照品线性方程计算5批样品中12种成分的含量。
结果： 吉尼德协中12种成分在检测范围内线性关系良好。5个批次吉尼德协中木兰花碱、药根碱、小檗碱、姜黄素、去甲氧基姜黄素、没食子酸、诃黎勒酸、阿魏酸4-O-β-D-吡喃葡萄糖苷、羟基红花黄色素A、芦丁、阿魏酸、鞣花酸的平均含量分别为3.18，0.20，1.71，0.52，0.22，15.93，7.56，3.29，0.07，0.20，1.47，2.89 mg·g-1。
结论： 本研究所建立的分析方法专属性强、灵敏度高，可为吉尼德协的质量控制提供参考。
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彭芳，女，硕士，主要从事药物化学成分分析与质量标准研究。E-mail: 2671302590@qq.com。
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