中草药

基于指纹图谱和多成分定量结合化学模式识别法评价不同产地狭叶薰衣草的质量

PDF下载

中草药 | 药材与资源 2026,57(17): 6946-6954

收起

中草药 |药材与资源 2026 , 57 (17) : 6946 -6954

基于指纹图谱和多成分定量结合化学模式识别法评价不同产地狭叶薰衣草的质量

全屏

王聪¹, 朱静平¹, 刘仟禧¹, 陈祥相¹, 张淋¹, 李红伟^1,2,3, 尹海龙⁴, 尹强⁴, 李凯^1,2,3

作者信息

通讯作者:

尹强

作者简介:

王聪: 王聪（1999—），女，河南新密人，硕士研究生，研究方向为中药饮片质量控制及临床应用研究。E-mail:wangcong662024@163.com

Quality evaluation of Lavandula angustifolia from different origins based on fingerprint combined with multi-component quantification and chemical pattern recognition

WANG Cong¹, ZHU Jingping¹, LIU Qianxi¹, CHEN Xiangxiang¹, ZHANG Lin¹, LI Hongwei^1,2,3, YIN Hailong⁴, YIN Qiang⁴, LI Kai^1,2,3

Affiliations

doi: 10.7501/j.issn.0253-2670.2026.17.024

文章导航

摘要

收起

目的建立不同产地狭叶薰衣草Lavandula angustifolia的HPLC指纹图谱及多成分含量测定方法，结合化学模式识别法评价狭叶薰衣草的质量，为其进一步研究和开发提供依据。方法采用ZORBAX Eclipse XDB-C₁₈色谱柱（250 mm×4.6 mm，5 μm），乙腈-0.1%磷酸水溶液为流动相，梯度洗脱，检测波长350 nm，柱温30 ℃，体积流量0.6 mL/min，进样量10 μL，建立新疆产地狭叶薰衣草HPLC指纹图谱并分析相似度，结合对照品对比指认化学成分并进行定量测定。通过Origin2025b、IBM SPSS Statistics 27和SIMCA 14.1软件进行聚类分析（hierarchical clustering analysis，HCA）、主成分分析（principal component analysis，PCA）及正交偏最小二乘法判别分析（orthogonal partial least squares-discriminant analysis，OPLS-DA），对不同产地狭叶薰衣草进行质量评价，筛选差异性标志物。结果建立了不同产地狭叶薰衣草的HPLC指纹图谱，共标定14个共有色谱峰，指认出其中5个成分，分别为绿原酸（峰2）、木犀草苷（峰8）、迷迭香酸（峰12）、木犀草素（峰13）、芹菜素（峰14）；15批样品相似度均在0.9以上；HCA将15批狭叶薰衣草分为3类；PCA提取了4个主成分，其方差累积贡献率为93.310%；PCA结果显示新疆霍城样品（批号YC23072402）质量最优；OPLS-DA筛选出狭叶薰衣草样品中木犀草素、迷迭香酸和芹菜素等7个质量差异标志物，狭叶薰衣草中木犀草素、迷迭香酸、芹菜素质量分数分别为0.018～0.230、0.380～3.095、0.144～0.481 mg/g。结论建立的新疆不同产地狭叶薰衣草HPLC指纹图谱及多成分含量测定方法简单、准确、重复性好，可用于狭叶薰衣草的质量评价，为其质量控制提供依据。

关键词

狭叶薰衣草 / 指纹图谱 / 木犀草素 / 迷迭香酸 / 芹菜素 / 化学模式识别 / 主成分分析

Abstract

收起

Objective To establish the HPLC fingerprint and multi-component content determination method of Lavandula angustifolia from different origins, and to evaluate the quality of L. angustifolia medicinal materials from different origins by combining chemical pattern recognition methods, providing a basis for further research and development. Methods A Zorbax Eclipse XDB-C₁₈ column (250 mm × 4.6 mm, 5 μm) was used, with acetonitrile-0.1% phosphoric acid solution as the mobile phase, and gradient elution. The detection wavelength was 350 nm, the column temperature was 30 ℃, the flow rate was 0.6 mL/min, and the injection volume was 10 μL. The HPLC fingerprint of L. angustifolia from Xinjiang was established and the similarity was analyzed. At the same time, the chemical components were identified by comparison with reference substances and quantitatively determined. Hierarchical clustering analysis (HCA), principal component analysis (PCA), and orthogonal partial least squares-discriminant analysis (OPLS-DA) were performed using Origin2025b, IBM SPSS Statistics 27 and SIMCA 14.1 software to evaluate the quality of L. angustifolia medicinal materials from different origins and screen the quality difference markers. Results The HPLC fingerprint chromatograms of L.angustifolia from different regions in Xinjiang were established. A total of 14 common chromatographic peaks were identified, and five components were recognized: Chlorogenic acid (peak 2), luteolin-7-O-glucoside (peak 8), rosmarinic acid (peak 12), luteolin (peak 13), and apigenin (peak 14).The similarity of 15 batches of samples was all above 0.9. HCA divided 15 batches of L. angustifolia into three categories. PCA extracted four principal components, with a cumulative contribution rate of 93.310%. PCA results showed that batch YC23072402 from Huocheng, Xinjiang, had the best quality. OPLS-DA identified seven quality difference markers, namely luteolin, rosmarinic acid and apigenin, in L. angustifolia samples. The contents of luteolin, rosmarinic acid and apigenin in L. angustifolia were 0.018—0.230, 0.380—3.095 and 0.144—0.481 mg/g respectively. Conclusion The HPLC fingerprint and multi-component content determination method for L. angustifolia from different origins is simple, accurate, and has good repeatability. It can be used for the quality evaluation of L. angustifolia from Xinjiang and provide a basis for its quality control.

Key words

Lavandula angustifolia Mill. / fingerprint chromatogram / luteolin / rosmarinic acid / apigenin / chemical pattern recognition / principal component analysis

引用本文

王聪, 朱静平, 刘仟禧, 陈祥相, 张淋, 李红伟, 尹海龙, 尹强, 李凯. 基于指纹图谱和多成分定量结合化学模式识别法评价不同产地狭叶薰衣草的质量. 中草药, 2026 , 57 (17) : 6946 -6954 . DOI: 10.7501/j.issn.0253-2670.2026.17.024

WANG Cong, ZHU Jingping, LIU Qianxi, CHEN Xiangxiang, ZHANG Lin, LI Hongwei, YIN Hailong, YIN Qiang, LI Kai. Quality evaluation of Lavandula angustifolia from different origins based on fingerprint combined with multi-component quantification and chemical pattern recognition[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (17) : 6946 -6954 . DOI: 10.7501/j.issn.0253-2670.2026.17.024

基金

收起

河南省杰出青年基金项目 (252300421028); 河南省高校科技创新团队 (26IRTSTHN018)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

韩凯乐, 张卫明, 杨建新, 等. 伊犁河谷有机薰衣草栽培技术[J]. 中国野生植物资源, 2013, 32(5): 59-61.
李慕春, 曹雪琴, 买吾兰江·买提努尔. 基于指纹图谱和化学计量学的两种薰衣草差异研究[J]. 中国野生植物资源, 2025, 44(4): 18-26.
欧文, 张海燕, 汪镇朝, 等. 薰衣草挥发油的化学成分及药理作用研究进展[J]. 中成药, 2022, 44(1): 170-176.
王伟娥, 田丽华, 张会竹, 等. 薰衣草有效成分研究进展[J]. 山东化工, 2020, 49(9): 55-56.
陈莘雨, 陈新梅. 薰衣草化学成分与药理作用研究进展[J]. 中华中医药杂志, 2022, 37(3): 1600-1604.
Yeung K S, Hernandez M, Mao J J, et al. Herbal medicine for depression and anxiety: A systematic review with assessment of potential psycho-oncologic relevance [J]. Phytother Res, 2018, 32(5): 865-891.
Linck V M, da Silva A L, Figueiró M, et al. Effects of inhaled linalool in anxiety, social interaction and aggressive behavior in mice [J]. Phytomedicine, 2010, 17(8/9): 679-683.
Sayout A, Ouarhach A, Rabie R, et al. Evaluation of antibacterial activity of Lavandula pedunculata subsp. Atlantica (Braun-blanq.) Romo essential oil and selected terpenoids against resistant bacteria strains-structure-activity relationships [J]. Chem Biodivers, 2020, 17(1): e1900496.
单孔荣, 王红丽, 姚乃捷, 等. 薰衣草精油与罗勒精油对痤疮致病菌的体外抑菌作用研究[J]. 广东药科大学学报, 2017, 33(5): 677-680.
Huang M Y, Liao M H, Wang Y K, et al. Effect of lavender essential oil on LPS-stimulated inflammation [J]. Am J Chin Med, 2012, 40(4): 845-859.
Zhao C Z, Sun J B, Fang C Y, et al. 1, 8-cineol attenuates LPS-induced acute pulmonary inflammation in mice [J]. Inflammation, 2014, 37(2): 566-572.
赵鑫, 鲍其泠, 王改香. 薰衣草精油成分分析及其抗菌和抗氧化活性研究[J]. 日用化学品科学, 2013, 36(4): 32-34.
Kozics K, Srancikova A, Sedlackova E, et al. Antioxidant potential of essential oil from Lavandula angustifolia in in vitro and ex vivo cultured liver cells [J]. Neoplasma, 2017, 64(4): 485-493.
Hritcu L, Cioanca O, Hancianu M. Effects of lavender oil inhalation on improving scopolamine-induced spatial memory impairment in laboratory rats [J]. Phytomedicine, 2012, 19(6): 529-534.
Velasco-Rodríguez R, Pérez-Hernández M G, Maturano-Melgoza J A, et al. The effect of aromatherapy with lavender (Lavandula angustifolia) on serum melatonin levels [J]. Complementary Ther Med, 2019, 47: 102208.
Coelho L S, Correa-Netto N F, Masukawa M Y, et al. Inhaled Lavandula angustifolia essential oil inhibits consolidation of contextual- but not tone-fear conditioning in rats [J]. J Ethnopharmacol, 2018, 215: 34-41.
古力娜·太来提, 任燕, 张莉. 新疆霍城薰衣草种植区土壤养分元素地球化学特征分析[J]. 新疆地质, 2024, 42(4): 640-645.
Yi D D, Liu X, Wang M H, et al. Rosmarinic acid alleviated intestinal barrier damage caused by Escherichia coli by regulating the gut microbiota and inhibiting the NF-κB signalling pathway in mice [J]. Food Funct, 2024, 15(23): 11740-11756.
Sim Y E, Kim C L, Kim D H, et al. Rosmarinic acid promotes cartilage regeneration through Sox9 induction via NF-κB pathway inhibition in mouse osteoarthritis progression [J]. Heliyon, 2024, 10(19): e38936.

2026年第57卷第17期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.7501/j.issn.0253-2670.2026.17.024

接收时间：2026-02-03
首发时间：2026-10-29

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2026-02-03

基金

作者信息

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.17.024

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT