中草药

苦蘵中1个新的甾体类化合物及其抗炎活性

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中草药 | 化学成分 2026,57(18): 7226-7232

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中草药 |化学成分 2026 , 57 (18) : 7226 -7232

苦蘵中1个新的甾体类化合物及其抗炎活性

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何月¹, 黄杰林¹, 沈惠玲¹, 徐丽丽¹, 尹俊林¹

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通讯作者:

徐丽丽

作者简介:

何月: 何月，女，硕士研究生，主要从事天然药物化学及其生物活性研究。E-mail:1679224399@qq.com

A new steroid from Physalis angulata and its anti-inflammatory activity

HE Yue¹, HUANG Jielin¹, SHEN Huiling¹, XU Lili¹, YIN Junlin¹

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doi: 10.7501/j.issn.0253-2670.2026.18.012

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目的研究苦蘵Physalis angulata全株的化学成分及其抗炎活性。方法综合运用多种色谱技术进行化合物的分离纯化，结合核磁共振、质谱及紫外光谱等波谱学方法鉴定其结构；采用SRB法评价化合物对小鼠巨噬细胞RAW264.7的细胞毒性，同时通过Griess法测定其体外抗炎活性。结果从苦蘵全株的甲醇提取物中分离得到5个化合物，分别鉴定为6-5β,6β:14α,17:14β,26-三环氧-13α,15β-二羟基-1-酮-11,15-环-13,14-开环麦角甾烷-2-烯-18,26-内酯（1）、酸浆苦素B（2）、酸浆苦素F（3）、酸浆苦素XI（4）、烟曲霉三唑酯A（5）。化合物2～5具有抑制RAW264.7细胞NO生成的活性，半数抑制浓度（median inhibition concentration，IC₅₀）值为（1.97±0.12）～（6.73±0.71）mmol/L。结论化合物1为新化合物，命名为5,6-环氧酸浆苦素IX；化合物4、5具有显著的抗炎活性。

关键词

酸浆属 / 苦蘵 / 甾体 / 5,6-环氧酸浆苦素IX / 酸浆苦素B / 酸浆苦素XI / 烟曲霉三唑酯A / 抗炎活性

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Objective To investigate the chemical constituents from the whole plant of Physalis angulata and their anti-inflammatory activities. Methods Various chromatographic techniques were employed for the isolation and purification of compounds, and their structures were elucidated by spectroscopic methods including NMR, MS, and UV. The cytotoxicity against mouse macrophage RAW264.7 cells was evaluated using the SRB assay, while the in vitro anti-inflammatory activity was determined via the Griess assay. Results Five compounds were isolated from the methanol extract of the whole plant of P. angulata and identified as 6- 5β,6β:14α,17:14β,26-triepoxy-13α,15β-dihydroxy-1-keto-11,15-cyclo-13,14-secoergostan-2-en-18,26-olide (1), physalin B (2), physalin F (3), physalin XI (4), and aspergillus triazolate A (5). Compounds 2—5 exhibited inhibitory effects on NO production in RAW264.7 cells, with IC₅₀ values ranging from (1.97 ± 0.12) to (6.73 ± 0.71) μmol/L. Conclusion Compound 1 is a new compound, named 5,6-epoxyphysalin IX. Compounds 4 and 5 showed significant anti-inflammatory activities.

Key words

Physalis L. / Physalis angulate L. / steroids / 5,6-epoxyphysalin IX / physalin B / physalin XI / aspergillus triazolate A / anti-inflammatory activity

引用本文

何月, 黄杰林, 沈惠玲, 徐丽丽, 尹俊林. 苦蘵中1个新的甾体类化合物及其抗炎活性. 中草药, 2026 , 57 (18) : 7226 -7232 . DOI: 10.7501/j.issn.0253-2670.2026.18.012

HE Yue, HUANG Jielin, SHEN Huiling, XU Lili, YIN Junlin. A new steroid from Physalis angulata and its anti-inflammatory activity[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (18) : 7226 -7232 . DOI: 10.7501/j.issn.0253-2670.2026.18.012

基金

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国家自然科学基金项目 (21768005); 国家自然科学基金项目 (82660967)

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2026年第57卷第18期

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doi: 10.7501/j.issn.0253-2670.2026.18.012

接收时间：2026-07-08
首发时间：2026-10-29

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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