中草药

基于多维度质量表征技术的牡荆叶标准汤剂质量评价

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刘喜婵 ¹, , 黄博 ¹, , 吴冰燕 ² , 蔡静 ² , 黄清泉 ¹, , 吴玉强 ³ , 罗轶 ¹,

中草药 | 药剂与工艺 2026,57(16): 6343-6355

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中草药 |药剂与工艺 2026 , 57 (16) : 6343 -6355

基于多维度质量表征技术的牡荆叶标准汤剂质量评价

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刘喜婵¹,, 黄博¹,, 吴冰燕², 蔡静², 黄清泉¹,, 吴玉强³, 罗轶¹,

作者信息

^1.广西壮族自治区药品检验研究院, 广西南宁 530021

^2.广西中医药大学, 广西南宁 530200

^3.广西中医药大学百年乐制药有限公司, 广西南宁 530023

^4.广西壮族自治区药品检验研究院, 广西南宁 530021

通讯作者:

吴玉强

作者简介:

刘喜婵: 刘喜婵(2000—),硕士研究生,主要从事中药制剂研究与开发研究。E-mail:liuxichan2023@stu.gxtcmu.edu.cn 黄博: 黄博(1981—),副主任药师,主要从事中药质量控制及安全研究。E-mail:137968757@qq.com

Quality evaluation of standard decoction of Viticis Negundo Folium based on multi-dimensional quality characterization technology

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出版时间: 2026-08-28 doi: 10.7501/j.issn.0253-2670.2026.16.011

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摘要

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目的基于特征图谱、网络药理学及定量分析方法，分析预测牡荆叶Viticis Negundo Folium标准汤剂的潜在质量标志物（quality markers，Q-Marker），构建牡荆叶标准汤剂的多维度质量评价体系。方法采用UPLC建立牡荆叶标准汤剂的特征图谱，色谱条件：色谱柱为Cortecs C₁₈柱（100 mm×2.1 mm，2.7 μm），流动相为乙腈-0.1%磷酸水溶液，梯度洗脱：0～9 min，3%～16%乙腈；9～21 min，16%～20%乙腈；21～22 min，20%～65%乙腈；22～23 min，65%乙腈；23～29 min，65%～95%乙腈；检测波长270 nm，柱温35 ℃，体积流量0.3 mL/min，进样体积2 μL。通过网络药理学预测候选Q-Marker的关键靶点和关键通路，构建“候选Q-Marker-关键靶点-关键通路”网络。对牡荆叶候选Q-Marker进行定量分析，明确牡荆叶候选Q-Marker在饮片-标准汤剂的量值传递规律。结果建立了牡荆叶标准汤剂的特征图谱，共标定出9个特征峰，通过对照品确认其中4个色谱峰（峰1、3、5、8），分别为对羟基苯甲酸、异荭草苷、异牡荆苷、芹菜素-7-O-β-D-葡萄糖醛酸苷（apigenin-7-O-β-D-glucuronide，AG）。经网络药理学分析，牡荆叶标准汤剂中对羟基苯甲酸、异荭草苷、异牡荆苷、AG，可能作用于基质金属蛋白酶2（matrix metalloproteinase 2，MMP2）、v-Rel禽网状内皮组织增殖病毒癌基因同源物A（v-Rel avian reticuloendotheliosis virus oncogene homolog A，RELA）、组蛋白去乙酰化酶1（histone deacetylase 1，HDAC1）等关键靶点，调控细胞衰老、晚期糖基化终末产物-受体（advanced glycation end products-receptor for advanced glycation end products signaling pathway，AGE-RAGE）、磷脂酰肌醇3-激酶-蛋白激酶B（phosphatidylinositol 3-kinase-protein kinase B，PI3K-Akt）等关键通路发挥治疗支气管炎的作用。对这4个成分进行定量分析，15批牡荆叶标准汤剂中的平均质量分数分别为18.98、9.13、4.72、1.64 mg/g，饮片中的平均质量分数分别为4.98、11.44、2.38、2.60 mg/g，从饮片到标准汤剂的平均转移率分别为118.83%、24.61%、54.75%、19.81%。初步确定对羟基苯甲酸、异荭草苷、异牡荆苷、AG为牡荆叶治疗支气管炎的Q-Marker。结论建立的质量评价方法准确可靠，量值传递均一稳定，结合网络药理学筛选的潜在Q-Marker可为牡荆叶标准汤剂的质量控制提供依据，为后续阐明其药效物质基础的作用机制提供支撑。

关键词

牡荆叶 / 标准汤剂 / 特征图谱 / 网络药理学 / 多维度质量表征技术 / 量值传递 / 对羟基苯甲酸 / 异荭草苷 / 异牡荆苷 / 芹菜素-7-O-β-D-葡萄糖醛酸苷 / 质量标志物

Abstract

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Objective To analyze and predict the quality markers (Q-Markers) of Mujingye (Viticis Negundo Folium, VNF) standard decoction based on characteristic chromatogram, network pharmacology, and quantitative analysis, and to construct a multi-dimensional quality evaluation system for VNF standard decoction. Methods UPLC was used to establish the characteristic chromatogram of VNF standard decoction. The chromatographic conditions were as follows: Cortecs C₁₈ column (100 mm×2.1 mm, 2.7 μm); the mobile phase consisted of acetonitrile (A) and 0.1% phosphoric acid (B), using gradient elution (0—9 min, 3%—16% A; 9—21 min, 16%—20% A; 21—22 min, 20%—65% A; 22—23 min, 65% A; 23—29 min, 65%—95% A); the detection wavelength was 270 nm; the column temperature was 35 ℃; the flow rate was 0.3 mL/min; and the injection volume was 2 μL. Network pharmacology was employed to obtain the key targets and key pathways of candidate Q-Markers, and the “candidate Q-Marker-key target-key pathway” network was constructed. Quantitative analysis was performed on the candidate Q-Markers of VNF to clarify the rules of quantity transfer of candidate Q-Markers from decoction pieces to standard decoction. Results The characteristic chromatogram of VNF standard decoction was established, and nine characteristic peaks were marked. Among them, four chromatographic peaks (peaks 1, 3, 5, and 8) were identified by reference substances as p-hydroxybenzoic acid, isoorientin, isovitexin, and apigenin-7-O-β-D-glucuronide (AG). Network pharmacology analysis revealed that p-hydroxybenzoic acid, isoorientin, isovitexin, and AG in VNF standard decoction could act on key targets such as matrix metalloproteinase 2 (MMP2), v-Rel avian reticuloendotheliosis virus oncogene homolog A(RELA), and histone deacetylase 1 (HDAC1), and regulate key pathways including cellular senescence, advanced glycation end products-receptor for advanced glycation end products (AGE-RAGE) signaling pathway, and phosphatidylinositol 3-kinase-protein kinase B (PI3K-Akt) signaling pathway to exert therapeutic effects against bronchitis. Quantitative analysis of these four components showed that their average contents in 15 batches of VNF standard decoction were 18.98, 9.13, 4.72, 1.64 mg/g, respectively, while their average contents in the decoction pieces were 4.98, 11.44, 2.38, 2.60 mg/g, respectively. The average transfer rates from decoction pieces to standard decoction were 118.83%, 24.61%, 54.75%, 19.81%, respectively. p-Hydroxybenzoic acid, isoorientin, isovitexin, and AG were preliminarily identified as the Q-Markers of VNF for treating bronchitis. Conclusion The established quality evaluation method is accurate and reliable, with uniform and stable quantity transfer. Combined with the Q-Markers screened by network pharmacology, it can provide a basis for the quality control of VNF standard decoction and support for the subsequent elucidation of the mechanism of action of its pharmacodynamic material basis.

Key words

Viticis Negundo Folium / standard decoction / characteristic chromatogram / network pharmacology / multi-dimensional quality characterization technology / quantity transfer / p-hydroxybenzoic acid / isoorientin / isovitexin / apigenin-7-O-β-D-glucuronide / Q-Marker

引用本文

刘喜婵, 黄博, 吴冰燕, 蔡静, 黄清泉, 吴玉强, 罗轶. 基于多维度质量表征技术的牡荆叶标准汤剂质量评价. 中草药, 2026 , 57 (16) : 6343 -6355 . DOI: 10.7501/j.issn.0253-2670.2026.16.011

LIU Xichan, HUANG Bo, WU Bingyan, CAI Jing, HUANG Qingquan, WU Yuqiang, LUO Yi. Quality evaluation of standard decoction of Viticis Negundo Folium based on multi-dimensional quality characterization technology[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (16) : 6343 -6355 . DOI: 10.7501/j.issn.0253-2670.2026.16.011

基金

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广西科技重大专项
河池市中央引导地方科技发展专项

参考文献

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文献

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参考文献引证文献

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中国药典[S]. 四部. 2025: 185.
陈士林, 刘昌孝, 张铁军, 等. 基于中药质量标志物和传统用法的中药饮片标准汤剂传承发展研究思路与建议[J]. 中草药, 2019, 50(19): 4519-4528.
王岩, 陈俊利, 冯文佳, 等. 全球、高SDI国家和中国＜5岁儿童1990—2019年下呼吸道感染疾病负担变化趋势[J]. 中国公共卫生, 2024, 40(7): 840-848.
Safiri S, Carson-Chahhoud K, Noori M, et al. Burden of chronic obstructive pulmonary disease and its attributable risk factors in 204 countries and territories, 1990—2019:Results from the Global Burden of Disease Study 2019[J]. Bmj, 2022, 378: e069679.
刘学东, 隋风翔, 刘菁. 吸入抗生素在支气管扩张症中的应用现状[J]. 中国临床药理学杂志, 2023, 39(14): 2107-2110.
Malo S, Poblador-Plou B, Prados-Torres A, et al. Poor congruence with guidelines in the use of antibiotics for acute bronchitis: A descriptive study based on electronic health records [J]. Fam Pract, 2016, 33(5): 471-475.
李曼曼, 黄正, 霍会霞, 等. 牡荆叶化学成分研究[J]. 世界科学技术—中医药现代化, 2015, 17(3): 578-582.
Umamaheswari G, Selvam K, Prakash P. Phytochemical profiling and antioxidant activity of aqueous leaf extract of Vitex negundo [J]. J Pharm Res Int, 2021, 33(23B): 22-32.
李曼曼. 牡荆叶抗炎活性成分研究[D]. 北京: 北京中医药大学, 2015.
Fang S T, Kong N N, Yan B F, et al. Chemical constituents and their bioactivities from the fruits of Vitex negundo var. cannabifolia [J]. Nat Prod Res, 2016, 30(24): 2856-2860.
刘昌孝, 陈士林, 肖小河, 等. 中药质量标志物(Q-Marker): 中药产品质量控制的新概念[J]. 中草药, 2016, 47(9): 1443-1457.
Zhang L X, Yi H L, Sang N. Sulfur dioxide-induced exacerbation of airway inflammation via reactive oxygen species production and the Toll-like receptor 4/nuclear factor-κB pathway in asthmatic mice [J]. Toxicol Ind Health, 2021, 37(9): 564-572.
Hu R, Wang M Q, Ni S H, et al. Salidroside ameliorates endothelial inflammation and oxidative stress by regulating the AMPK/NF-κB/NLRP3 signaling pathway in AGEs-induced HUVECs [J]. Eur J Pharmacol, 2020, 867: 172797.
Reynaert N L, Vanfleteren L E G W, Perkins T N. The AGE-RAGE axis and the pathophysiology of multimorbidity in COPD [J]. J Clin Med, 2023, 12(10):3366.
Li Y J, Du L, Ye K Q, et al. Akt inhibition sensitizes acute leukemia cells to S63845-induced apoptosis [J]. Hematology, 2023, 28(1): 2214465.
Li Y, Jiang Y, Wan Y C, et al. Medroxyprogestogen enhances apoptosis of SKOV-3 cells via inhibition of the PI3K/Akt signaling pathway [J]. J Biomed Res, 2013, 27(1): 43-50.
Zhou B H, Tan P P, Jia L S, et al. PI3K/Akt signaling pathway involvement in fluoride-induced apoptosis in C2C12 cells [J]. Chemosphere, 2018, 199: 297-302.
Ji K Y, Wu S H, Yuan J Y, et al. L-selenomethylselenocysteine exerts inhibitory effects on the progression of esophageal cancer by targeting the PI3K/Akt signaling pathway [J]. Recent Pat Anti Cancer Drug Discov, 2026, 21(2): 161-172.
Wang M, Liu M, Huang Y J, et al. Differential gene expression and methylation analysis of melanoma in TCGA database to further study the expression pattern of KYNU in melanoma [J]. J Pers Med, 2022, 12(8): 1209.
Wang B, Han J, Elisseeff JH, et al. The senescence-associated secretory phenotype and its physiological and pathological implications [J]. Nat Rev Mol Cell Biol. 2024, 25(12): 958-978.
Li D, Luo Y F, Chen X L, et al. NF-κB and poly (ADP-ribose) polymerase 1 form a positive feedback loop that regulates DNA repair in acute myeloid leukemia cells [J]. Mol Cancer Res, 2019, 17(3): 761-772.
Kwon J W, Kwon H K, Shin H J, et al. Activating transcription factor 3 represses inflammatory responses by binding to the p65 subunit of NF-κB [J]. Sci Rep, 2015, 5: 14470.
Qu H, Zhang P, Tang Y, et al. Epstein-Barr virus-driven immunosuppression in nasopharyngeal carcinoma: A comprehensive review of viral mechanisms, spatial tumor ecosystems, and precision therapeutics [J]. Front Immunol, 2026, 17:1875687.
赵婕. 基于亚胺类共价有机骨架磁性复合材料的制备及其在食品样品前处理中的应用[D]. 烟台: 鲁东大学, 2024.
Sirichokworrakit S, Rimkeeree H, Chantrapornchai W, et al. Comparative study on conventional, accelerated solvent extraction and ultrasonic-assisted extraction of total phenolic and anthocyanin contents and antioxidant activities from riceberry bran [J]. Agr Nat Res, 2022, 56(2): 243-254.
杨云霞, 李可, 王易佳, 等. 对羟基苯甲酸-水为主体晶格的管状包合物的合成与晶体结构[J]. 北京师范大学学报: 自然科学版, 2010, 46(2): 160-165.
Ginwala R, Bhavsar R, Chigbu D I, et al. Potential role of flavonoids in treating chronic inflammatory diseases with a special focus on the anti-inflammatory activity of apigenin [J]. Antioxidants, 2019, 8(2): 35.
陆松侠, 黄茸茸, 胡健力, 等. 基于IL-33/ST2信号通路研究补肺汤对慢性支气管炎大鼠肺组织炎症因子表达的影响[J]. 中国老年学杂志, 2023, 43(23): 5826-5830.
Liu M X, Li T, Wang W G, et al. Regulatory effect of isovitexin on MAPK/NF-κB signal in mice with acute ulcerative colitis [J]. J Asian Nat Prod Res, 2023, 25(8): 765-782.
Chen T T, Xu L Q, Gao Z G, et al. Isovitexin accelerates diabetic wound repair via coordinated angiogenesis and collagen remodeling: Mechanistic insights from cellular and streptozotocin-induced SD rat models [J]. Tissue Cell, 2025, 97: 103100.
杨兰珠, 李诗怡, 孙雨伟, 等. 异荭草苷通过MAPK/ FoxO信号通路减轻LPS诱导的RAW264.7细胞炎症反应[J]. 安徽农业大学学报, 2024, 51(1): 132-137.

2026年第57卷第16期

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doi: 10.7501/j.issn.0253-2670.2026.16.011

接收时间：2026-01-21
首发时间：2026-10-22
出版时间：2026-08-28

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广西科技重大专项

河池市中央引导地方科技发展专项

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^1.广西壮族自治区药品检验研究院, 广西南宁 530021

^2.广西中医药大学, 广西南宁 530200

^3.广西中医药大学百年乐制药有限公司, 广西南宁 530023

^4.广西壮族自治区药品检验研究院, 广西南宁 530021

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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