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黄花菜叶多波长指纹图谱的建立及一测多评法测定其中5种指标成分

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中草药 | 药材与资源 2026,57(9): 3572-3578

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中草药 |药材与资源 2026 , 57 (9) : 3572 -3578

黄花菜叶多波长指纹图谱的建立及一测多评法测定其中5种指标成分

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张菊¹, 张乾年¹, 曹阳洋¹, 韩佳冰¹, 宋平平¹, 王燕萍¹, 胡芳弟¹

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通讯作者:

胡芳弟

作者简介:

张菊: 张菊(1996-),女,硕士研究生,主要从事中药成分分离分析研究。Tel:18419151426 E-mail:zhangju19@lzu.edu.cn

Study on multi-wavelength fingerprints of Hemerocallis citrina leaves and five index components by QAMS

ZHANG Ju, ZHANG Qiannian, CAO Yangyang, HAN Jiabing, SONG Pingping, WANG Yanping, HU Fangdi

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doi: 10.7501/j.issn.0253-2670.2026.09.025

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目的通过指纹图谱分析黄花菜Hemerocallis citrina叶的特征成分，进一步对其中指标成分绿原酸、芦丁、槲皮素-3-O-葡萄糖苷、槲皮苷、槲皮素进行含量测定，以期为黄花菜叶利用及其质量评价提供依据。方法采用乙醇提取法制备黄花菜叶提取物，选用色谱柱DIKMA spursil C₁₈柱（250 mm×4.6 mm，5 mm），流动相为乙腈-0.5%乙酸水，进样量10 mL，检测波长为190～400 nm，柱温30 ℃，体积流量1 mL/min，采集时间80 min；基于最大吸收波长原则，分别在时间段0～5.5 min和23～48 min选择258 nm，5.5～23 min选择300 nm，48～80 min选择370 nm作为检测波长，构建了3波长切换指纹图谱。采用一测多评法（quantitative analysis of multi-components by single-marker，QAMS）测定黄花菜叶乙醇提取物中绿原酸、芦丁、槲皮素-3-O-葡萄糖苷、槲皮苷、槲皮素5种酚类成分的含量。结果 10批黄花菜叶的指纹图谱相似度在0.90以上，所构建的3波长切换指纹图谱共包括42个共有峰（占总峰面积90%以上），其中峰11、25、26、32、37被鉴定为绿原酸、芦丁、槲皮素-3-O-葡萄糖苷、槲皮苷、槲皮素。以槲皮素（S）为内参物，QAMS法获得绿原酸、芦丁、槲皮素-3-O-葡萄糖苷、槲皮苷的相对校正因子分别为1.402、1.896、1.268、1.221。10批黄花菜叶中上述5种成分的平均含量为0.079、0.086、0.374、0.325、0.037 mg/g。外标法与QAMS法的含量测定结果无显著性差异。结论 3波长切换指纹图谱较任何单一波长指纹图谱所包含的色谱峰数均有显著提升，所构建的多波长切换指纹图谱可用于黄花菜叶化学成分表征，进一步构建的QAMS法可以一次性实现绿原酸、芦丁、槲皮素-3-O-葡萄糖苷、槲皮苷、槲皮素5种成分的含量测定，为全面评价黄花菜叶质量提供了方法学依据和参考。

关键词

黄花菜植物叶 / HPLC指纹图谱 / 多波长切换 / 多成分含量测定 / 一测多评 / 绿原酸 / 芦丁 / 槲皮素-3-O-葡萄糖苷 / 槲皮苷 / 槲皮素

Abstract

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Objective Huanghuacai (Hemerocallis citrina) is a traditional vegetable with anti-inflammatory and anti-depressant effects. Its leaves are widely used in Taiwan and other regions to promote sleep, however, there are few reports on its chemical composition. This article aims to analyze the characteristic components of H. citrina leaves by fingerprint spectrum and to further determine the content of the index components, including chlorogenic acid, rutin, quercetin-3-O-glucoside, quercitrin, and quercetin, providing a basis for better utilization and quality evaluation of H. citrina leaves. Methods Firstly, the ethanol extraction method was used to prepare H. citrina leaves extract. Chromatographic separation was performed by DIKMA spursil C₁₈ column (250 mm × 4.6 mm, 5 mm) maintained at 30 ℃ and the mobile phase was acetonitrile-0.5% acetic acid water. The injection volume was 10 mL, the detection wavelength was 190—400 nm and the flow rate was 1 mL/min. Based on the principle of maximum absorption wavelength, the wavelengths of 258 nm was selected in two periods of 0—5.5 and 23—48 min, the wavelengths of 300 and 370 nm were selected in two periods of 5.5—23 and 48—80 min respectively, and a three-wavelength switching fingerprint was constructed. Secondly,a quantitative analysis of multi-components by single marker (QAMS) was used to determine the contents of chlorogenic acid, rutin, quercetin-3-O-glucoside, quercetin, and quercetin in ethanol extract of H. citrina leaves. Results The similarity of fingerprint spectrums of 10 batches of H. citrina leaves was above 0.90, and the three-wavelength switching fingerprint spectrums included 42 common peaks (accounting for more than 90% of the total peak area), of which 11, 25, and 26, 32 and 37 were identified as chlorogenic acid, rutin, quercetin-3-O-glucoside, quercitrin, and quercetin. With quercetin (S) as the internal reference, the relative correction factors of chlorogenic acid, rutin, quercetin-3-O-glucoside, and quercitrin were obtained by the QAMS as 1.402, 1.896, 1.268, 1.221. The average contents of the above five components in 10 batches of H. citrina leaves are 0.079, 0.086, 0.374, 0.325, 0.037 mg/g. There was no significant difference between the external standard method and QAMS. Conclusion The three-wavelength switching fingerprint spectrum constructed in this study has significantly increased the number of chromatographic peaks contained in any single wavelength fingerprint spectrum and can be used to characterize the chemical composition of H. citrina leaves. The QAMS further constructed can be used to determine the content of chlorogenic acid, rutin, quercetin-3-O-glucoside, quercitrin, and quercetin at one time. This study provides a methodological basis and reference for the comprehensive evaluation of H. citrina leaves quality.

Key words

Hemerocallis citrina Baroni. leaves / HPLC fingerprint spectrum / multi-wavelength switching / quantitative analysis of multi-components / QAMS / chlorogenic acid / rutin / quercetin-3-O-glucoside / quercitrin

引用本文

张菊, 张乾年, 曹阳洋, 韩佳冰, 宋平平, 王燕萍, 胡芳弟. 黄花菜叶多波长指纹图谱的建立及一测多评法测定其中5种指标成分. 中草药, 2026 , 57 (9) : 3572 -3578 . DOI: 10.7501/j.issn.0253-2670.2026.09.025

ZHANG Ju, ZHANG Qiannian, CAO Yangyang, HAN Jiabing, SONG Pingping, WANG Yanping, HU Fangdi. Study on multi-wavelength fingerprints of Hemerocallis citrina leaves and five index components by QAMS[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (9) : 3572 -3578 . DOI: 10.7501/j.issn.0253-2670.2026.09.025

基金

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河南省重点研发专项(251111310500);河南省重点研发专项(31111312700);河南省重点研发专项(241111310200);国家自然科学基金资助项目(82104329);国家自然科学基金资助项目(32401226);中国博士后科学基金特别资助项目(2014T170252);河南省国际科技合作项日(242102521056);河南中医药大学科研苗圃工程资助项目(MP2024-56)

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2026年第57卷第9期

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doi: 10.7501/j.issn.0253-2670.2026.09.025

接收时间：2025-12-01
首发时间：2026-09-09

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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