中草药

基于数据挖掘和网络药理学探析酸枣仁-人参药对的镇静催眠作用机制

PDF下载

中草药 | 数据挖掘与循证医学 2026,57(9): 3495-3507

收起

中草药 |数据挖掘与循证医学 2026 , 57 (9) : 3495 -3507

基于数据挖掘和网络药理学探析酸枣仁-人参药对的镇静催眠作用机制

全屏

卢春阳¹, 徐志佳¹, 李雪¹, 孟令鲲¹, 张红印¹, 李光哲^1,2, 严铭铭^1,3

作者信息

通讯作者:

李光哲

作者简介:

卢春阳: 卢春阳,硕士研究生,从事天然药物化学及新药开发研究。E-mail:24204892094@stu.ccucm.edu.cn

Analysis on sedative hypnotic mechanism of Ziziphi Spinosae Semen-Ginseng Radix et Rhizoma herb pair based on data mining and network pharmacology

LU Chunyang, XU Zhijia, LI Xue, MENG Lingkun, ZHANG Hongyin, LI Guangzhe, YAN Mingming

Affiliations

doi: 10.7501/j.issn.0253-2670.2026.09.019

文章导航

摘要

收起

目的挖掘中国古今含酸枣仁-人参药对的复方，并进一步分析药对常治疾病及机制。方法应用数据挖掘收集含有酸枣仁-人参药对的复方并统计药对常治疾病及药理作用，运用网络药理学预测酸枣仁-人参药对常治疾病机制，并且使用分子对接技术验证候选化合物与潜在靶标的结合能力，最后运用酶联免疫吸附法和免疫组织化学技术验证所发现的酸枣仁-人参药对失眠与神经系统功能的调节机制。结果检索得到含酸枣仁-人参药对复方596首，经复方药理作用挖掘统计发现酸枣仁-人参药对主要发挥镇静催眠作用，常用于治疗失眠症等疾病。网络药理学分析结果显示，酸枣仁-人参药对可能通过调控神经活性配体-受体相互作用、γ-氨基丁酸（gamma-aminobutyric acid，GABA）能突触及炎症相关通路发挥镇静催眠作用。分子对接结果表明，多种核心活性成分与关键靶点具有较好的结合活性。体内外实验结果显示，酸枣仁-人参药对可改善谷氨酸诱导的SH-SY5Y细胞损伤，提高失眠模型大鼠相关神经递质水平，降低炎症因子水平，并促进海马及下丘脑中γ-氨基丁酸A型受体亚基α1（gamma-aminobutyric acid type A receptor subunit alpha1，GABRA1）和γ-氨基丁酸A型受体亚基γ2（gamma-aminobutyric acid type A receptor subunit gamma 2，GABRG2）的表达。结论通过对酸枣仁-人参药对常治疾病的挖掘和发挥镇静催眠机制的探索，揭示了酸枣仁-人参药对的主要用药方向及作用机制，为该药对后续的深入研究及临床应用提供了一定的参考。

关键词

人参 / 酸枣仁 / 药对 / 数据挖掘 / 网络药理学 / 分子对接 / 失眠症

Abstract

收起

Objective Discovering the ancient and modern Chinese compound prescriptions containing Suanzaoren (Ziziphi Spinosae Semen)-(Renshen) Ginseng Radix et Rhizoma herb pair (ZSS-GRR) and further analyzing the diseases commonly treated by the pairs and their therapeutic mechanisms. Methods Data mining method was applied to collect the compound formulas containing ZSS-GRR and count the commonly treated diseases and pharmacological effects, network pharmacology was applied to predict the mechanism of ZSS-GRR herb pair, and molecular docking technique was used to verify the binding ability of candidate compounds to potential targets, and finally enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were used to validate the discovered mechanisms of ZSS-GRR for insomnia and nervous system function. Results A total of 596 compound formulations containing the ZSS-GRR were searched. Statistical analysis of the pharmacological effects of these formulations revealed that the ZSS-GRR primarily exerts sedative and hypnotic effects and is commonly used to treat conditions such as insomnia. Network pharmacology analysis indicates that the ZSS-GRR may exert its sedative and hypnotic effects by regulating neuroactive ligand-receptor interactions, gamma-aminobutyric acid (GABA) ergic synapses, and inflammation-related pathways. Molecular docking results suggest that multiple core active components exhibit strong binding affinity with key targets. Experimental results demonstrate that the ZSS-GRR combination can alleviate glutamate-induced damage in SH-SY5Y cells, increase levels of relevant neurotransmitters in rats with an insomnia model, reduce levels of inflammatory factors, and promote the expression of gamma-aminobutyric acid type A receptor subunit alpha 1 (GABRA1) and gamma-aminobutyric acid type A receptor subunit gamma 2 (GABRG2) in the hippocampus and hypothalamus. Conclusion Through the exploration of the commonly treated diseases and the sedative-hypnotic mechanism of ZSS-GRR, the main direction and mechanism of action of ZSS-GRR have been revealed, which provides certain valuable references for the subsequent in-depth research and clinical application of the pair.

Key words

Ginseng Radix et Rhizoma / Ziziphi Spinosae Semen / herb pair / data mining / network pharmacology / molecular docking / insomnia

引用本文

卢春阳, 徐志佳, 李雪, 孟令鲲, 张红印, 李光哲, 严铭铭. 基于数据挖掘和网络药理学探析酸枣仁-人参药对的镇静催眠作用机制. 中草药, 2026 , 57 (9) : 3495 -3507 . DOI: 10.7501/j.issn.0253-2670.2026.09.019

LU Chunyang, XU Zhijia, LI Xue, MENG Lingkun, ZHANG Hongyin, LI Guangzhe, YAN Mingming. Analysis on sedative hypnotic mechanism of Ziziphi Spinosae Semen-Ginseng Radix et Rhizoma herb pair based on data mining and network pharmacology[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (9) : 3495 -3507 . DOI: 10.7501/j.issn.0253-2670.2026.09.019

基金

收起

国家自然科学基金资助项目(82274360);山西省中医药管理局资助项目(2024ZYY2A032);山西省中医药创新团队(zyytd2024020);山西省中药功效物质研究与利用重点实验室资助(202105D121009);经方扶阳山西省重点实验室开放课题研究基金资助(CPSY202501)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

张诗嘉.基于数据挖掘分析刘朝霞教授治疗胃食管反流病的用药规律[D].哈尔滨:黑龙江中医药大学,2022.
Zhang H, Liu W, Qi S M, et al. Improved effect of fresh ginseng paste(Radix ginseng-Ziziphus Jujube)on hyperuricemia based on network pharmacology and molecular docking[J]. Front Pharmacol, 2022, 13:955219.
姚玉静,张书敏,任艳艳,等.酸枣仁提取物、龙眼肉提取物、γ-氨基丁酸和酪蛋白水解物复配制剂改善睡眠功能[J].食品工业科技, 2023, 44(7):406-410.
Li F T, Yang D, Song F Y, et al. In vitro effects of ginseng and the seed of Zizyphus jujuba var. spinosa on gut microbiota of rats with spleen deficiency[J]. Chem Biodivers, 2020, 17(9):e2000199.
Liu M, Cai Y Y, Li F T, et al. Metabolomics and network pharmacological analysis to explore the mechanism of ginseng treatment for spleen-qi deficiency[J]. J Sep Sci,2022, 45(24):4427-4438.
Bian Z H, Zhang W M, Tang J Y, et al. Mechanisms underlying the action of Ziziphi Spinosae Semen in the treatment of insomnia:A study involving network pharmacology and experimental validation[J]. Front Pharmacol, 2021, 12:752211.
徐敬娅,马欣雨,赵佳鹤,等.人参、附子药对配伍及其温阳功效研究概况[J].河北中医, 2022, 44(7):1224-1228.
王媛.基于“脑肠轴”探讨药对人参-酸枣仁对心脾两虚失眠大鼠的影响[D].沈阳:辽宁中医药大学, 2022.
吴艳,杜娟,张晓雷. UHPLC-TOF-MS结合网络药理学与分子对接探讨人参-酸枣仁药对治疗帕金森的作用机制[J].中国现代应用药学, 2025, 42(20):3559-3568.
张经北.基于数据挖掘分析古代名医治疗不寐医案中用药规律[D].长春:长春中医药大学, 2023.
任海琴,孔祥鹏,王颖莉.基于古今方剂数据挖掘的酸枣仁-远志药对配伍特点及外延分析[J].中草药,2022, 53(13):4065-4074.
中国药典[S].一部. 2025:431.
冉小峰,胡长鸿.全国中药成药处方集[M].北京:人民卫生出版社, 1962:34-73.
Ru J L, Li P, Wang J N, et al. TCMSP:A database of systems pharmacology for drug discovery from herbal medicines[J]. J Cheminf, 2014, 6(1):13.
Xue R C, Fang Z, Zhang M X, et al. TCMID:Traditional Chinese medicine integrative database for herb molecular mechanism analysis[J]. Nucleic Acids Res, 2012, 41(D1):D1089-D1095.
Upton D H, Liu J, George S M, et al. High-throughput in vitro drug screening and in vivo studies identify fenretinide as a brain-penetrant DMG therapeutic[J]. Neuro Oncol,2025, 27(7):1813-1828.
Zhang Y Q, Guo Q Y, Li Q Y, et al. Main active constituent identification in Guanxinjing Capsule, a traditional Chinese medicine, for the treatment of coronary heart disease complicated with depression[J]. Acta Pharmacol Sin, 2018, 39(6):975-987.
Daina A, Michielin O, Zoete V. SwissTargetPrediction:Updated data and new features for efficient prediction of protein targets of small molecules[J]. Nucleic Acids Res,2019, 47(W1):W357-W364.
Safran M, Dalah I, Alexander J, et al. GeneCards Version3:The human gene integrator[J]. Database J Biol Databases Curation, 2010, 2010:baq020.
Queralt-Rosinach N, Piñero J, BravoÀ, et al. DisGeNETRDF:Harnessing the innovative power of the Semantic Web to explore the genetic basis of diseases[J].Bioinformatics, 2016, 32(14):2236-2238.
Wishart D S, Feunang Y D, Guo A C, et al. DrugBank 5.0:A major update to the DrugBank database for 2018[J].Nucleic Acids Res, 2018, 46(D1):D1074-D1082.
Zhou Y, Zhang Y T, Lian X C, et al. Therapeutic target database update 2022:Facilitating drug discovery with enriched comparative data of targeted agents[J]. Nucleic Acids Res, 2022, 50(D1):D1398-D1407.
Whirl-Carrillo M, McDonagh E M, Hebert J M, et al.Pharmacogenomics knowledge for personalized medicine[J]. Clin Pharmacol Ther, 2012, 92(4):414-417.
Soudy M, Anwar A M, Ali Ahmed E, et al. UniprotR:Retrieving and visualizing protein sequence and functional information from Universal Protein Resource(UniProt knowledgebase)[J]. J Proteom, 2020, 213:103613.
Szklarczyk D, Gable A L, Lyon D, et al. STRING v11:Protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets[J]. Nucleic Acids Res, 2019,47(D1):D607-D613.
Sherman B T, Hao M, Qiu J, et al. DAVID a web server for functional enrichment analysis and functional annotation of gene lists(2021 update)[J]. Nucleic Acids Res, 2022,50(W1):W216-W221.
翁小建,谈毅,高秀飞.基于网络药理学与生物信息学预测大枣抗焦虑、抗抑郁的潜在活性成分及作用机制[J].浙江中西医结合杂志, 2022, 32(4):359-363.
Bae G Y, Ahn Y, Hong K B, et al. Sleep-enhancing effect of water extract from jujube(Zizyphus jujuba Mill.)seeds fermented by Lactobacillus brevis L32[J]. Foods, 2023,12(15):2864.
Hua Y, Guo S, Xie H, et al. Ziziphus jujuba Mill. var.spinosa(Bunge)Hu ex H. F. Chou seed ameliorates insomnia in rats by regulating metabolomics and intestinal flora composition[J]. Front Pharmacol, 2021, 12:653767.
Cui X F, Zhang S M, He L, et al. In vitro biotransformation of Ziziphi Spinosae Semen saponins by gut microbiota from healthy and insomniac groups[J]. Int J Mol Sci, 2025,26(9):4011.
Kim S, Chen J, Cheng T J, et al. PubChem 2023 update[J]. Nucleic Acids Res, 2023, 51(D1):D1373-D1380.
K S B, Charmi B, Chunxiao B, et al. RCSB Protein Data Bank(RCSB.org):Delivery of experimentally-determined PDB structures alongside one million computed structure models of proteins from artificial intelligence/machine learning[J]. Nucleic Acids Res, 2022, 51(D1):D488-D508.
肖凤琴,王欣,刘晖,等.基于熵权法和灰色关联度法优选人参-酸枣仁抗氧化活性最佳配比[J].食品与机械, 2023, 39(6):173-179.
肖凤琴.人参-酸枣仁药对药效物质基础及对ROT诱导的帕金森模型的神经保护作用研究[D].长春:长春中医药大学, 2023.
郭海波,王慧.对氯苯丙氨酸在动物失眠模型中的应用概述[J].中国比较医学杂志, 2019, 29(6):135-140.
丁环宇,洪勇良,齐凤军,等.“君臣佐使”于临床的创新应用[J].时珍国医国药, 2022, 33(5):1178-1179.
Zhao N, Zhao Y J, An F R, et al. Network analysis of comorbid insomnia and depressive symptoms among psychiatric practitioners during the COVID-19 pandemic[J]. J Clin Sleep Med, 2023, 19(7):1271-1279.
Selbæk-Tungevåg S, Selbæk G, Strand B H, et al.Insomnia and risk of dementia in a large population-based study with 11-year follow-up:The HUNT study[J]. J Sleep Res, 2023, 32(4):e13820.
Yang Y N, Su W Q, Zang C C, et al. Traditional Chinese medicines(TCMs)with varied meridians(Gui-Jing)differentially alleviate the adverse impact of Coptis chinensis on gut microbiota[J]. J Ethnopharmacol, 2023,307:116256.
赵萱,陈云慧,郑明月,等.基于数据挖掘的含人参-附子药对方剂的组方规律分析[J].中草药, 2021,53(4):1083-1091.
Xiao F Q, Shao S, Zhang H Y, et al. Neuroprotective effect of Ziziphi Spinosae Semen on rats with pchlorophenylalanine-induced insomnia via activation of GABAA receptor[J]. Front Pharmacol, 2022, 13:965308.
de Oliveira Zanuso B, de Oliveira dos Santos A R, Miola V F B, et al. Panax ginseng and aging related disorders:A systematic review[J]. Exp Gerontol, 2022, 161:111731.
Li R, Wang L, Wang X, et al. Simultaneous determination of four monoamine neurotransmitters and seven effective components of Zaoren Anshen prescription in rat tissue using UPLC-MS/MS[J]. Curr Pharm Anal, 2021, 17(1):67-80.
Qiao T, Wang Y, Liang K, et al. Effects of the Radix Ginseng and Semen Ziziphi Spinosae drug pair on the GLU/GABA-GLN metabolic cycle and the intestinal microflora of insomniac rats based on the brain-gut axis[J]. Front Pharmacol, 2022, 13:1094507.

2026年第57卷第9期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.7501/j.issn.0253-2670.2026.09.019

接收时间：2025-09-23
首发时间：2026-09-09

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2025-09-23

基金

作者信息

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.09.019

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT