Article(id=1304415020125810735, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414997581427653, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.08.027, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1759680000000, receivedDateStr=2025-10-06, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788926375657, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788926375657, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788926375657, creator=13701087609, updateTime=1788926375657, updator=13701087609, issue=Issue{id=1304414997581427653, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='8', pageStart='2877', pageEnd='3260', issueExtLink='null', onlineDate='null', pubDate='1777305600000', pubDateStr='2026-04-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788926370282, creator='13701087609', updateTime=1788926758667, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304416626649096991, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414997581427653, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304416626649096992, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414997581427653, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=3163, endPage=3172, ext={EN=ArticleExt(id=1304415021954527281, articleId=1304415020125810735, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Study on spatiotemporal distribution characteristics of index components and processing technology optimization in Rubus chingii, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective To investigate the spatiotemporal distribution characteristics of kaempferol-3-O-rutinoside and ellagic acid, the medicinal marker components of Rubus chingii, and optimize the processing technology to improve their contents based on the findings. Methods Matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF IMS) was used for imaging analysis of R. chingii fruits, and high-performance liquid chromatography (HPLC) was employed for quantitative determination of the marker components in fresh fruits and medicinal materials processed by different technologies. Results Kaempferol-3-O-rutinoside was mainly distributed in the outer layer of the fruit, while ellagic acid and its precursors were enriched in the receptacle. Both components exhibited the highest content in green fruits and gradually decreased with fruit ripening. Compared with oven-drying, the sun-drying process significantly increased ellagic acid content by 68.71% but reduced kaempferol-3-O-rutinoside content by 41.30%. Drying after steam moistening at 120 ℃ for 30 min followed by drying effectively improved ellagic acid content without significant negative impacts on kaempferol-3-O-rutinoside content or the appearance of medicinal materials. Conclusion The tissue-specific distribution of the marker components in R. chingii is a key factor affecting their content changes during processing. Timely harvesting of green fruits and optimization of processing technology (short-time steam moistening) can synergistically improve the quality of medicinal materials., authors=LI Xiaobai, JIN Shuifeng, SHAO Ruoxuan, JIN Liang, LUO Laiyin, FU Shunhua, WANG Yuping, JIANG Ling, authorsList=LI Xiaobai, JIN Shuifeng, SHAO Ruoxuan, JIN Liang, LUO Laiyin, FU Shunhua, WANG Yuping, JIANG Ling, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304415021862252592, articleId=1304415020125810735, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=覆盆子中指标成分时空分布特征及其加工工艺优化研究, columnId=1304140203967410414, journalTitle=中草药, columnName=药材与资源, runingTitle=null, highlight=null, articleAbstract=目的 探究覆盆子Rubus chingii中药用指标成分山柰酚-3-O-芸香糖苷和鞣花酸的时空分布特征，基于优化加工工艺提高其含量。方法 采用基质辅助激光解吸/电离飞行时间质谱成像（MALDI-TOF IMS）技术对覆盆子果实进行成像分析，并用HPLC法测定覆盆子鲜果及日晒、烘干处理后药材的指标成分山柰酚-3-O-芸香糖苷和鞣花酸含量。结果 山柰酚-3-O-芸香糖苷主要分布于果实外层，鞣花酸及其前体富集于果托，两者均在青果中含量最高，并随着果实成熟而逐渐下降。与烘干相比，日晒工艺使鞣花酸含量显著增加68.71%，但山柰酚-3-O-芸香糖苷含量降低41.30%。120℃蒸汽回润30 min后干燥可有效提升鞣花酸含量，且对山柰酚-3-O-芸香糖苷及药材品相无显著负面影响。结论 覆盆子指标成分的组织特异性分布是加工过程中含量变化的关键因素，适时采收青果并优化加工工艺（短时蒸汽回润）可协同提升药材质量。, authors=李小白1, 金水丰2, 邵若玄3, 金亮1, 罗来印4, 付顺华5, 王玉萍6, 姜玲6, authorsList=李小白, 金水丰, 邵若玄, 金亮, 罗来印, 付顺华, 王玉萍, 姜玲, authorCompany=1 浙江省农业科学院, 浙江 杭州 310021; 2 杭州市农业技术推广中心(杭州市植保植检中心), 浙江 杭州 310020; 3 嵊州市农业技术推广中心, 浙江 绍兴 312400; 4 淳安县永印家庭农场, 浙江 杭州 311700; 5 浙江农林大学, 浙江 杭州 311300; 6 淳安县农业农村发展服务中心, 浙江 杭州 311700, correspAuthors=姜玲, authorNote=李小白: 李小白,男,博士,副研究员,主要从事覆盆子和蓝莓等高价值保健水果的研究。E-mail:hufanfan1982815@outlook.com, correspAuthorsNote=null, 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Ding H Y. Extracts and constituents of Rubus chingii with 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity[J]. Int J Mol Sci, 2011, 12(6):3941-3949. Zhang T T, Lu C L, Jiang J G, et al. Bioactivities and extraction optimization of crude polysaccharides from the fruits and leaves of Rubus chingii Hu[J]. Carbohydr Polym, 2015, 130:307-315. Sun N, Wang Y, Liu Y, et al. A new ent-labdane diterpene saponin from the fruits of Rubus chingii[J]. Chem Nat Compd, 2013, 49(1):49-53. Jantas D, Malarz J, Le T N, et al. Neuroprotective properties of kempferol derivatives from Maesa membranacea against oxidative stress-induced cell damage:An association with cathepsin D inhibition and PI3K/Akt activation[J]. Int J Mol Sci, 2021, 22(19):10363. 中国药典[S]. 一部. 2020:1088. Hering N A, Luettig J, Jebautzke B, et al. The punicalagin metabolites ellagic acid and urolithin a exert different strengthening and anti-inflammatory effects on tight junction-mediated intestinal barrier function in vitro[J]. Front Pharmacol, 2021, 12:610164. Chang S Y, Ko Y, Kim M J. Regulatory mechanisms of kaempferol on iNOS expression in RINm5F β-cells under exposure to interleukin-1β[J]. Heliyon, 2023, 9(4):e14818. Huwait E, Ayoub M, Karim S. Investigation of the molecular mechanisms underlying the antiatherogenic actions of kaempferol in human THP-1 macrophages[J]. Int J Mol Sci, 2022, 23(13):7461. 何佳, 周越美, 赵智慧, 等. 不同加工方法与贮存时间对覆盆子含量测定指标的影响[J]. 医药导报, 2022, 41(5):693-697. Li K Y, Zeng M L, Li Q L, et al. Identification of polyphenolic composition in the fruits of Rubus chingii Hu and its antioxidant and antiproliferative activity on human bladder cancer T24 cells[J]. J Food Meas Charact, 2019, 13(1):51-60. Li X B, Sun J, Chen Z, et al. Characterization of carotenoids and phenolics during fruit ripening of Chinese raspberry (Rubus chingii Hu)[J]. RSC Adv, 2021, 11(18):10804-10813. Cid-Ortega S, Monroy-Rivera J A. Extraction of kaempferol and its glycosides using supercritical fluids from plant sources:A review[J]. Food Technol Biotechnol, 2018, 56(4):480-493. de Alencar Filho José M T, Pedrita A S, Emanuella C V P, et al. Flavonoids as photoprotective agents:A systematic review[J]. J Med Plants Res, 2016, 10(47):848-864. Li X B, Jiang J Y, Chen Z, et al. Transcriptomic, proteomic and metabolomic analysis of flavonoid biosynthesis during fruit maturation in Rubus chingii Hu[J]. Front Plant Sci, 2021, 12:706667. Lin Y, Jiang X C, Zhu S, et al. Multi-omics combined with MALDI mass spectroscopy imaging reveals the mechanisms of biosynthesis of characteristic compounds in Tetrastigma hemsleyanum Diels et Gilg[J]. Front Plant Sci, 2024, 14:1294804. Klewicka E, Sójka M, Klewicki R, et al. Ellagitannins from raspberry (Rubus idaeus L.) fruit as natural inhibitors of Geotrichum candidum[J]. Molecules, 2016, 21(7):908. Zhao W R, Zheng M Z, Li X, et al. Fruit astringency:Mechanisms, technologies, and future directions[J]. Horticulturae, 2025, 11(6):45-56. Sharifi-Rad J, Quispe C, Castillo C M S, et al. Ellagic acid:A review on its natural sources, chemical stability, and therapeutic potential[J]. Oxid Med Cell Longev, 2022, 2022:3848084. Li X B, Wang Y, Jin L, et al. Development of fruit color in Rubus chingii Hu (Chinese raspberry):A story about novel offshoots of anthocyanin and carotenoid biosynthesis[J]. Plant Sci, 2021, 311:110996. Kähkönen M, Kylli P, Ollilainen V, et al. Antioxidant activity of isolated ellagitannins from red raspberries and cloudberries[J]. J Agric Food Chem, 2012, 60(5):1167-1174. Raya-Morquecho E M, Aguilar-Zarate P, Sepúlveda L, et al. Ellagitannins and their derivatives:A review on the metabolization, absorption, and some benefits related to intestinal health[J]. Microbiol Res, 2025, 16(6):78-86. Larrosa M, Tomás-Barberán F A, Espín J C. The dietary hydrolysable tannin punicalagin releases ellagic acid that induces apoptosis in human colon adenocarcinoma Caco-2 cells by using the mitochondrial pathway[J]. J Nutr Biochem, 2006, 17(9):611-625. Dall'Acqua S, Miolo G, Innocenti G, et al. The photodegradation of quercetin:Relation to oxidation[J]. Molecules, 2012, 17(8):8898-8907. He B H, Dai L H, Jin L, et al. Bioactive components, pharmacological effects, and drug development of traditional herbal medicine Rubus chingii Hu (Fu-Pen-Zi)[J]. Front Nutr, 2023, 9:1052504. Dehghanian Z, Habibi K, Dehghanian M, et al. Reinforcing the bulwark:Unravelling the efficient applications of plant phenolics and tannins against environmental stresses[J]. Heliyon, 2022, 8(3):e09094. Qian C, Li H F, Hou Z N, et al. Effects of different drying methods on Rubus chingii Hu fruit during processing[J]. Heliyon, 2024, 10(2):e24512. Teslić N, Santos F, Oliveira F, et al. Simultaneous hydrolysis of ellagitannins and extraction of ellagic acid from defatted raspberry seeds using natural deep eutectic solvents (NADES)[J]. Antioxidants, 2022, 11(2):254. Hu J X, Li X, Yu Q T, et al. Understanding the impact of pectin physicochemical variation on browning of simulated Maillard reaction system in thermal and storage processing[J]. Int J Biol Macromol, 2023, 240:124347.)
Objective To investigate the spatiotemporal distribution characteristics of kaempferol-3-O-rutinoside and ellagic acid, the medicinal marker components of Rubus chingii, and optimize the processing technology to improve their contents based on the findings. Methods Matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF IMS) was used for imaging analysis of R. chingii fruits, and high-performance liquid chromatography (HPLC) was employed for quantitative determination of the marker components in fresh fruits and medicinal materials processed by different technologies. Results Kaempferol-3-O-rutinoside was mainly distributed in the outer layer of the fruit, while ellagic acid and its precursors were enriched in the receptacle. Both components exhibited the highest content in green fruits and gradually decreased with fruit ripening. Compared with oven-drying, the sun-drying process significantly increased ellagic acid content by 68.71% but reduced kaempferol-3-O-rutinoside content by 41.30%. Drying after steam moistening at 120 ℃ for 30 min followed by drying effectively improved ellagic acid content without significant negative impacts on kaempferol-3-O-rutinoside content or the appearance of medicinal materials. Conclusion The tissue-specific distribution of the marker components in R. chingii is a key factor affecting their content changes during processing. Timely harvesting of green fruits and optimization of processing technology (short-time steam moistening) can synergistically improve the quality of medicinal materials.
Key words
Rubus chingii Hu
/
kaempferol-3-O-rutinoside
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ellagic acid
/
processing technology
/
mass spectrometry imaging
LI Xiaobai, JIN Shuifeng, SHAO Ruoxuan, JIN Liang, LUO Laiyin, FU Shunhua, WANG Yuping, JIANG Ling.
Study on spatiotemporal distribution characteristics of index components and processing technology optimization in Rubus chingii[J].
Chinese Traditional and Herbal Drugs,
2026
, 57
(8)
: 3163
-3172
.
DOI: 10.7501/j.issn.0253-2670.2026.08.027
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杭州市农业产业技术专家团队项目 (202407TD17)
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中国药典[S]. 一部. 2025:411-412. Ding H Y. Extracts and constituents of Rubus chingii with 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity[J]. Int J Mol Sci, 2011, 12(6):3941-3949. Zhang T T, Lu C L, Jiang J G, et al. Bioactivities and extraction optimization of crude polysaccharides from the fruits and leaves of Rubus chingii Hu[J]. Carbohydr Polym, 2015, 130:307-315. Sun N, Wang Y, Liu Y, et al. A new ent-labdane diterpene saponin from the fruits of Rubus chingii[J]. Chem Nat Compd, 2013, 49(1):49-53. Jantas D, Malarz J, Le T N, et al. Neuroprotective properties of kempferol derivatives from Maesa membranacea against oxidative stress-induced cell damage:An association with cathepsin D inhibition and PI3K/Akt activation[J]. Int J Mol Sci, 2021, 22(19):10363. 中国药典[S]. 一部. 2020:1088. Hering N A, Luettig J, Jebautzke B, et al. The punicalagin metabolites ellagic acid and urolithin a exert different strengthening and anti-inflammatory effects on tight junction-mediated intestinal barrier function in vitro[J]. Front Pharmacol, 2021, 12:610164. Chang S Y, Ko Y, Kim M J. Regulatory mechanisms of kaempferol on iNOS expression in RINm5F β-cells under exposure to interleukin-1β[J]. Heliyon, 2023, 9(4):e14818. Huwait E, Ayoub M, Karim S. Investigation of the molecular mechanisms underlying the antiatherogenic actions of kaempferol in human THP-1 macrophages[J]. Int J Mol Sci, 2022, 23(13):7461. 何佳, 周越美, 赵智慧, 等. 不同加工方法与贮存时间对覆盆子含量测定指标的影响[J]. 医药导报, 2022, 41(5):693-697. Li K Y, Zeng M L, Li Q L, et al. Identification of polyphenolic composition in the fruits of Rubus chingii Hu and its antioxidant and antiproliferative activity on human bladder cancer T24 cells[J]. J Food Meas Charact, 2019, 13(1):51-60. Li X B, Sun J, Chen Z, et al. Characterization of carotenoids and phenolics during fruit ripening of Chinese raspberry (Rubus chingii Hu)[J]. RSC Adv, 2021, 11(18):10804-10813. Cid-Ortega S, Monroy-Rivera J A. Extraction of kaempferol and its glycosides using supercritical fluids from plant sources:A review[J]. Food Technol Biotechnol, 2018, 56(4):480-493. de Alencar Filho José M T, Pedrita A S, Emanuella C V P, et al. Flavonoids as photoprotective agents:A systematic review[J]. J Med Plants Res, 2016, 10(47):848-864. Li X B, Jiang J Y, Chen Z, et al. Transcriptomic, proteomic and metabolomic analysis of flavonoid biosynthesis during fruit maturation in Rubus chingii Hu[J]. Front Plant Sci, 2021, 12:706667. Lin Y, Jiang X C, Zhu S, et al. Multi-omics combined with MALDI mass spectroscopy imaging reveals the mechanisms of biosynthesis of characteristic compounds in Tetrastigma hemsleyanum Diels et Gilg[J]. Front Plant Sci, 2024, 14:1294804. Klewicka E, Sójka M, Klewicki R, et al. Ellagitannins from raspberry (Rubus idaeus L.) fruit as natural inhibitors of Geotrichum candidum[J]. Molecules, 2016, 21(7):908. Zhao W R, Zheng M Z, Li X, et al. Fruit astringency:Mechanisms, technologies, and future directions[J]. Horticulturae, 2025, 11(6):45-56. Sharifi-Rad J, Quispe C, Castillo C M S, et al. Ellagic acid:A review on its natural sources, chemical stability, and therapeutic potential[J]. Oxid Med Cell Longev, 2022, 2022:3848084. Li X B, Wang Y, Jin L, et al. Development of fruit color in Rubus chingii Hu (Chinese raspberry):A story about novel offshoots of anthocyanin and carotenoid biosynthesis[J]. Plant Sci, 2021, 311:110996. Kähkönen M, Kylli P, Ollilainen V, et al. Antioxidant activity of isolated ellagitannins from red raspberries and cloudberries[J]. J Agric Food Chem, 2012, 60(5):1167-1174. Raya-Morquecho E M, Aguilar-Zarate P, Sepúlveda L, et al. Ellagitannins and their derivatives:A review on the metabolization, absorption, and some benefits related to intestinal health[J]. Microbiol Res, 2025, 16(6):78-86. Larrosa M, Tomás-Barberán F A, Espín J C. The dietary hydrolysable tannin punicalagin releases ellagic acid that induces apoptosis in human colon adenocarcinoma Caco-2 cells by using the mitochondrial pathway[J]. J Nutr Biochem, 2006, 17(9):611-625. Dall'Acqua S, Miolo G, Innocenti G, et al. The photodegradation of quercetin:Relation to oxidation[J]. Molecules, 2012, 17(8):8898-8907. He B H, Dai L H, Jin L, et al. Bioactive components, pharmacological effects, and drug development of traditional herbal medicine Rubus chingii Hu (Fu-Pen-Zi)[J]. Front Nutr, 2023, 9:1052504. Dehghanian Z, Habibi K, Dehghanian M, et al. Reinforcing the bulwark:Unravelling the efficient applications of plant phenolics and tannins against environmental stresses[J]. Heliyon, 2022, 8(3):e09094. Qian C, Li H F, Hou Z N, et al. Effects of different drying methods on Rubus chingii Hu fruit during processing[J]. Heliyon, 2024, 10(2):e24512. Teslić N, Santos F, Oliveira F, et al. Simultaneous hydrolysis of ellagitannins and extraction of ellagic acid from defatted raspberry seeds using natural deep eutectic solvents (NADES)[J]. Antioxidants, 2022, 11(2):254. Hu J X, Li X, Yu Q T, et al. Understanding the impact of pectin physicochemical variation on browning of simulated Maillard reaction system in thermal and storage processing[J]. Int J Biol Macromol, 2023, 240:124347.
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