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首荟通便胶囊通过调节肠道菌群、抑制TLR4/MyD88/NF-κB通路及恢复神经递质平衡改善卒中后抑郁

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中草药 | 药理与临床 2026,57(7): 2583-2601

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中草药 |药理与临床 2026 , 57 (7) : 2583 -2601

首荟通便胶囊通过调节肠道菌群、抑制TLR4/MyD88/NF-κB通路及恢复神经递质平衡改善卒中后抑郁

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李若秋¹, 潘成龙¹, 王宇婷¹, 王融融¹, 曹庆宇², 沈萌萌³, 叶青⁴, 周继栋³, 牛德军³, 潘丽红³, 姚景春³, 曾振³, 张贵民¹

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曾振

作者简介:

李若秋: 李若秋,硕士研究生,研究方向为药理学。E-mail:369495752@qq.com 潘成龙: 潘成龙,硕士研究生,研究方向为药理学。E-mail:ll07140605@163.com

Shouhui Tongbian Capsule alleviate post-stroke depression by modulating gut microbiota, inhibiting TLR4/MyD88/NF-κB signaling pathway and restoring neurotransmitter homeostasis

LI Ruoqiu, PAN Chenglong, WANG Yuting, WANG Rongrong, CAO Qingyu, SHEN Mengmeng, YE Qing, ZHOU Jidong, NIU Dejun, PAN Lihong, YAO Jingchun, ZENG Zhen, ZHANG Guimin

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doi: 10.7501/j.issn.0253-2670.2026.07.014

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目的采用多组学策略探讨首荟通便胶囊（Shouhui Tongbian Capsule，SHTB）对卒中后抑郁（post-stroke depression，PSD）的治疗效果及其作用机制。方法通过光化学诱导脑卒中联合慢性不可预见性温和应激（chronic unpredictable mild stress，CUMS）建立PSD小鼠模型。通过行为学测试评价抑郁样行为，并通过组织学分析评估神经元和髓鞘结构。综合运用肠道菌群16S rDNA测序、粪便和脑组织代谢组学、蛋白组学、神经递质检测及Western blotting等方法，系统解析其潜在机制。此外，通过抗生素清除肠道菌群，以验证SHTB的作用是否依赖于肠道微生物。结果 SHTB能显著改善PSD小鼠的抑郁样行为及神经元形态。16S rDNA测序分析结果显示，SHTB可恢复肠道菌群平衡，表现为有益菌属（如肠球菌属Enterococcus）的增加以及促炎菌属（如颤螺菌属Oscillibacter、丹毒丝菌科Erysipelotrichaceae）的减少。代谢组学结果表明，SHTB可纠正脂肪酸代谢紊乱，特别是亚油酸和花生四烯酸通路，并降低神经炎症相关代谢物水平。蛋白组学和神经递质检测进一步揭示，SHTB能够恢复5-羟色胺（5-hydroxytryptamine，5-HT）、γ-氨基丁酸（γ-aminobutyric acid，GABA）、多巴胺（dopamine，DA）和去甲肾上腺素（norepinephrine，NE）水平（P＜0.05），并减少谷氨酸的异常累积（P＜0.05），同时抑制TLR4/MyD88/NF-κB炎症通路的过度激活（P＜0.05）。在抗生素清除肠道菌群后，上述效应均显著减弱，表明SHTB的功效具有肠道菌群依赖性。结论 SHTB能通过肠道菌群依赖性的方式，重塑肠道菌群、修复肠道屏障、调控脂肪酸代谢、抑制TLR4/MyD88/NF-κB通路介导的外周及中枢炎症反应、并恢复关键神经递质平衡，多维度改善肠-脑轴功能，从而发挥抗PSD及神经保护作用。

关键词

卒中后抑郁 / 首荟通便胶囊 / 肠道菌群 / 脂肪酸代谢 / 神经炎症 / 神经递质失衡 / TLR4/MyD88/NF-κB通路

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Objective To explore the therapeutic effect and mechanism of Shouhui Tongbian Capsule (首荟通便胶囊, SHTB) on post-stroke depression (PSD) using a multi-omics strategy. Methods A mouse model of PSD was established by photochemical induction of stroke combined with chronic unpredictable mild stress (CUMS). Depressive-like behaviors were evaluated by behavioral tests, and neuronal and myelin sheath structures were assessed by histological analysis. A comprehensive approach integrating 16S rDNA sequencing of gut microbiota, metabolomics of feces and brain tissues, proteomics, neurotransmitter detection and Western blotting was employed to systematically elucidate the potential mechanism. Additionally, gut microbiota was depleted by antibiotics to verify whether the effect of SHTB is dependent on intestinal microbes. Results SHTB significantly improved depressive-like behaviors and neuronal morphology in PSD mice. 16S rDNA sequencing analysis showed that SHTB restored the balance of gut microbiota, characterized by an increase in beneficial genera (e.g., Enterococcus) and a decrease in pro-inflammatory genera (e.g., Oscillibacter, Erysipelotrichaceae). Metabolomic results indicated that SHTB could correct fatty acid metabolism disorders, particularly linoleic acid and arachidonic acid pathways, and reduce the levels of neuroinflammation-related metabolites. Proteomics and neurotransmitter detection further revealed that SHTB could restore the levels of serotonin (5-HT), γ-aminobutyric acid (GABA), dopamine (DA) and norepinephrine (NE) (P < 0.05), reduce the abnormal accumulation of glutamate (P < 0.05), and inhibit the excessive activation of TLR4/MyD88/NF-κB inflammatory pathway (P < 0.05). After depletion of gut microbiota by antibiotics, the above effects were significantly attenuated, indicating that the efficacy of SHTB was gut microbiota-dependent. Conclusion SHTB could restore the gut microbiota, repair the intestinal barrier, regulate fatty acid metabolism, inhibit TLR4/MyD88/NF-κB pathway-mediated peripheral and central inflammatory responses, and restore the balance of key neurotransmitters through a gut microbiota-dependent mechanism. This multi-dimensional improvement in the gut-brain axis function contributes to its anti-PSD and neuroprotective effects.

Key words

post-stroke depression / Shouhui Tongbian Capsule / gut microbiota / fatty acid metabolism / neuroinflammation / neurotransmitter imbalance / TLR4/MyD88/NF-κB pathway

引用本文

李若秋, 潘成龙, 王宇婷, 王融融, 曹庆宇, 沈萌萌, 叶青, 周继栋, 牛德军, 潘丽红, 姚景春, 曾振, 张贵民. 首荟通便胶囊通过调节肠道菌群、抑制TLR4/MyD88/NF-κB通路及恢复神经递质平衡改善卒中后抑郁. 中草药, 2026 , 57 (7) : 2583 -2601 . DOI: 10.7501/j.issn.0253-2670.2026.07.014

LI Ruoqiu, PAN Chenglong, WANG Yuting, WANG Rongrong, CAO Qingyu, SHEN Mengmeng, YE Qing, ZHOU Jidong, NIU Dejun, PAN Lihong, YAO Jingchun, ZENG Zhen, ZHANG Guimin. Shouhui Tongbian Capsule alleviate post-stroke depression by modulating gut microbiota, inhibiting TLR4/MyD88/NF-κB signaling pathway and restoring neurotransmitter homeostasis[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (7) : 2583 -2601 . DOI: 10.7501/j.issn.0253-2670.2026.07.014

基金

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山东省自然科学基金资助项目 (ZR2022LZY021)

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2026年第57卷第7期

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doi: 10.7501/j.issn.0253-2670.2026.07.014

接收时间：2025-11-18
首发时间：2026-09-09

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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