Article(id=1304414955709690590, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414955046985824, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.07.002, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1769443200000, receivedDateStr=2026-01-27, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788926360299, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788926360299, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788926360299, creator=13701087609, updateTime=1788926360299, updator=13701087609, issue=Issue{id=1304414955046985824, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='7', pageStart='2445', pageEnd='2876', issueExtLink='null', onlineDate='null', pubDate='1775923200000', pubDateStr='2026-04-12', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788926360140, creator='13701087609', updateTime=1788926711174, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304416427457409395, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414955046985824, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304416427457409396, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414955046985824, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=2456, endPage=2462, ext={EN=ArticleExt(id=1304414955990708960, articleId=1304414955709690590, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=A new diterpene compound from Alstonia mairei and its antifungal activity, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective To investigate the chemical constituents from the twigs and leaves of Alstonia mairei and to evaluate their antifungal activity. Methods The separation and purification were conducted by a variety of chromatographic separation techniques, including MCI, silica gel, Sephadex LH-20 gel column chromatography, and preparative high-performance liquid chromatography. The chemical structures of the isolated compounds were elucidated by spectroscopic methods, including mass spectrometry, nuclear magnetic resonance, and electronic circular dichroism (ECD). The antifungal activity of the selected compounds was evaluated against Candida albicans CMCC98001 and Aspergillus niger R330 using the microbroth dilution method. Results A total of eight compounds were isolated from the twigs and leaves of A. mairei, including one new compound, ent-12-hydroxy-16-methylol-atisane-3-one (1), and seven known compounds, 17-acetylsarpagine (2), 1,2,3,4-tetrahydro-1-oxo-β-carboline (3), vinorine N4-oxide (4), vinorine (5), yohimbine (6), hancockinol (7), ent-16β, 17-dihydroxyatlsan-3-one (8). The MICs of compound 1 and 8 against A. niger R330 were 12.5 and 6.25 μg/mL, respectively. Conclusion Compound 1 is a new diterpenoid named alstomanoid A, and compounds 3, 7 and 8 are isolated from the genus of A. mairei for the first time. Compounds 1 and 8 show antifungal effect against A. niger R330., authors=ZHANG Ruihan, QIN Wenxiu, YU Shuo, LI Chang, PEI Yuehu, YANG Yihui, authorsList=ZHANG Ruihan, QIN Wenxiu, YU Shuo, LI Chang, PEI Yuehu, YANG Yihui, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304414955923600095, articleId=1304414955709690590, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=羊角棉中1个新的二萜类化合物及其抗真菌活性, columnId=1304140187169214944, journalTitle=中草药, columnName=化学成分, runingTitle=null, highlight=null, articleAbstract=目的 研究羊角棉Alstonia mairei枝叶的化学成分及抗真菌活性。方法 采用MCI、硅胶、羟丙基葡聚糖凝胶及制备高效液相等多种色谱方法进行分离纯化,利用质谱、核磁共振和ECD等谱学方法鉴定化合物结构。采用微量肉汤稀释法测试了部分单体化合物对白色念珠菌(Candida albicans CMCC98001)和黑曲霉菌(Aspergillus niger R330)的抗菌活性。结果 从羊角棉枝叶中分离并鉴定了8个化合物,包括1个新化合物ent-12-羟基-16-羟甲基阿替烷-3-酮(1)以及7个已知化合物,分别为17-乙酰蛇根精(2)、1,2,3,4-四氢-1-氧-β-咔啉(3)、维诺任碱N₄-氧化物(4)、维诺任碱(5)、育亨宾(6)、华北白前醇(7)、ent-16β,17-二羟基阿替烷-3-酮(8)。化合物18对黑曲霉菌的最低抑菌浓度(minimum inhibitory concentration,MIC)值分别为12.5、6.25 μg/mL。结论 化合物1为新的二萜类化合物,命名为替烷醇酮A,化合物378是首次从鸡骨常山属植物中分离获得。化合物18对黑曲霉菌有一定的抑制作用。, authors=张瑞函1, 秦文秀1, 于硕1, 李畅1, 裴月湖1, 杨异卉1, authorsList=张瑞函, 秦文秀, 于硕, 李畅, 裴月湖, 杨异卉, authorCompany=1 哈尔滨医科大学药学院, 黑龙江 哈尔滨 150081, correspAuthors=裴月湖, authorNote=张瑞函: 张瑞函,女,硕士研究生,研究方向为天然药物化学。E-mail:ruihanz2000@126.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=2s1fl+PucZg8uXNk2oXSkw==, pdfFileSize=1432562, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, 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Mohammed A E, Abdul-Hameed Z H, Alotaibi M O, et al.Chemical diversity and bioactivities of monoterpene indole alkaloids(MIAs)from six Apocynaceae Genera[J].Molecules, 2021, 26(2):488.
Ali M, Sultana S, Mir S R. Chemical constituents from the Alstonia scholaris stem bark, Eclipta prostrata aerial parts and Morus alba stem bark[J]. Eur J Pharm Med Res, 2020,7(4):511-521.
Zhang L, Hua Z Q, Song Y, et al. Monoterpenoid indole alkaloids from Alstonia rupestris with cytotoxic,antibacterial and antifungal activities[J]. Fitoterapia,2014, 97:142-147.
Yan T L, Han D X, Hu J, et al. Monoterpenoid indole alkaloids from Alstonia mairei and their cytotoxicity[J]. J Asian Nat Prod Res, 2017, 19(6):550-556.
Zhao Y L, Shang J H, Pu S B, et al. Effect of total alkaloids from Alstonia scholaris on airway inflammation in rats[J].J Ethnopharmacol, 2016, 178:258-265.
Chua L S, Abdullah F I, Awang M A. Potential of natural bioactive C-glycosyl flavones for antidiabetic properties[M] //Bioactive Natural Products. Amsterdam:Elsevier,2020:241-261.
Li Y Q, Cai J, Cheng J X, et al. Alstomairines D–G:New monoterpenoid indole alkaloids with cytotoxic activity from Alstonia mairei[J]. J Mol Struct, 2022, 1253:132277.
Pierce C G, Uppuluri P, Tristan A R, et al. A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing[J]. Nat Protoc, 2008, 3(9):1494-1500.
Li X A, Zhang D Z, Onda M, et al. Ent-kauranoid diterpenes from Artemisia sacrorum[J]. J Nat Prod, 1990,53(3):657-661.
Chen W M, Yan Y P, Wang Y J, et al. Isolation and identification of three new alkaloids from the roots of Alstonia yunnanensis Diels Indiana, USA[J]. Acta Pharm Sin, 1985, 20(12):906-912.
TissandiéL, Viciana S, Brevard H, et al. Towards a complete characterisation of guaiacwood oil[J].Phytochemistry, 2018, 149:64-81.
Feng T, Li Y, Cai X H, et al. Monoterpenoid indole alkaloids from Alstonia yunnanensis[J]. J Nat Prod, 2009,72(10):1836-1841.
吴昊,宋京风,范堃,等.云南萝芙木根中吲哚生物碱及其抗菌活性[J].中草药, 2023, 54(4):1033-1042.
李文静,洪博,赵春杰.萝芙木化学成分的分离与鉴定[J].中国药房, 2013, 24(3):256-258.
Konda Y, Iguchi M, Harigaya Y, et al. Hancokinol, a novel triterpene, from Cynanchum hancokianum[J].Tetrahedron Lett, 1990, 31(37):5315-5318.
王妍,梁旭博,赵珍珠.泽漆全草中二萜类成分研究[J].中草药, 2022, 53(15):4625-4633.)
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羊角棉中1个新的二萜类化合物及其抗真菌活性
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张瑞函, 秦文秀, 于硕, 李畅, 裴月湖, 杨异卉
中草药 | 化学成分 2026,57(7): 2456-2462
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中草药 |化学成分 2026 , 57 (7) : 2456 -2462
羊角棉中1个新的二萜类化合物及其抗真菌活性
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张瑞函, 秦文秀, 于硕, 李畅, 裴月湖, 杨异卉
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通讯作者:
裴月湖
作者简介:
张瑞函: 张瑞函,女,硕士研究生,研究方向为天然药物化学。E-mail:ruihanz2000@126.com
A new diterpene compound from Alstonia mairei and its antifungal activity
ZHANG Ruihan, QIN Wenxiu, YU Shuo, LI Chang, PEI Yuehu, YANG Yihui
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doi: 10.7501/j.issn.0253-2670.2026.07.002
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目的 研究羊角棉Alstonia mairei枝叶的化学成分及抗真菌活性。方法 采用MCI、硅胶、羟丙基葡聚糖凝胶及制备高效液相等多种色谱方法进行分离纯化,利用质谱、核磁共振和ECD等谱学方法鉴定化合物结构。采用微量肉汤稀释法测试了部分单体化合物对白色念珠菌(Candida albicans CMCC98001)和黑曲霉菌(Aspergillus niger R330)的抗菌活性。结果 从羊角棉枝叶中分离并鉴定了8个化合物,包括1个新化合物ent-12-羟基-16-羟甲基阿替烷-3-酮(1)以及7个已知化合物,分别为17-乙酰蛇根精(2)、1,2,3,4-四氢-1-氧-β-咔啉(3)、维诺任碱N₄-氧化物(4)、维诺任碱(5)、育亨宾(6)、华北白前醇(7)、ent-16β,17-二羟基阿替烷-3-酮(8)。化合物18对黑曲霉菌的最低抑菌浓度(minimum inhibitory concentration,MIC)值分别为12.5、6.25 μg/mL。结论 化合物1为新的二萜类化合物,命名为替烷醇酮A,化合物378是首次从鸡骨常山属植物中分离获得。化合物18对黑曲霉菌有一定的抑制作用。
羊角棉  /  二萜类化合物  /  生物碱类化合物  /  抗真菌活性  /  替烷醇酮A  /  1,2,3,4-四氢-1-氧-β-咔啉  /  华北白前醇  /  ent- 16β,17-二羟基阿替烷-3-酮
Objective To investigate the chemical constituents from the twigs and leaves of Alstonia mairei and to evaluate their antifungal activity. Methods The separation and purification were conducted by a variety of chromatographic separation techniques, including MCI, silica gel, Sephadex LH-20 gel column chromatography, and preparative high-performance liquid chromatography. The chemical structures of the isolated compounds were elucidated by spectroscopic methods, including mass spectrometry, nuclear magnetic resonance, and electronic circular dichroism (ECD). The antifungal activity of the selected compounds was evaluated against Candida albicans CMCC98001 and Aspergillus niger R330 using the microbroth dilution method. Results A total of eight compounds were isolated from the twigs and leaves of A. mairei, including one new compound, ent-12-hydroxy-16-methylol-atisane-3-one (1), and seven known compounds, 17-acetylsarpagine (2), 1,2,3,4-tetrahydro-1-oxo-β-carboline (3), vinorine N4-oxide (4), vinorine (5), yohimbine (6), hancockinol (7), ent-16β, 17-dihydroxyatlsan-3-one (8). The MICs of compound 1 and 8 against A. niger R330 were 12.5 and 6.25 μg/mL, respectively. Conclusion Compound 1 is a new diterpenoid named alstomanoid A, and compounds 3, 7 and 8 are isolated from the genus of A. mairei for the first time. Compounds 1 and 8 show antifungal effect against A. niger R330.
Alstonia mairei Lévl.  /  diterpenoid  /  alkaloids  /  antifungal activity  /  alstomanoid A  /  1,2,3,4-tetrahydro-1-oxo-β-carboline  /  hancockinol  /  ent-16β,17-dihydroxyatlsan-3-one
张瑞函, 秦文秀, 于硕, 李畅, 裴月湖, 杨异卉. 羊角棉中1个新的二萜类化合物及其抗真菌活性. 中草药, 2026 , 57 (7) : 2456 -2462 . DOI: 10.7501/j.issn.0253-2670.2026.07.002
ZHANG Ruihan, QIN Wenxiu, YU Shuo, LI Chang, PEI Yuehu, YANG Yihui. A new diterpene compound from Alstonia mairei and its antifungal activity[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (7) : 2456 -2462 . DOI: 10.7501/j.issn.0253-2670.2026.07.002

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Wang C M, Yeh K L, Tsai S J, et al. Anti-proliferative activity of triterpenoids and sterols isolated from Alstonia scholaris against non-small-cell lung carcinoma cells[J].Molecules, 2017, 22(12):2119.
Mohammed A E, Abdul-Hameed Z H, Alotaibi M O, et al.Chemical diversity and bioactivities of monoterpene indole alkaloids(MIAs)from six Apocynaceae Genera[J].Molecules, 2021, 26(2):488.
Ali M, Sultana S, Mir S R. Chemical constituents from the Alstonia scholaris stem bark, Eclipta prostrata aerial parts and Morus alba stem bark[J]. Eur J Pharm Med Res, 2020,7(4):511-521.
Zhang L, Hua Z Q, Song Y, et al. Monoterpenoid indole alkaloids from Alstonia rupestris with cytotoxic,antibacterial and antifungal activities[J]. Fitoterapia,2014, 97:142-147.
Yan T L, Han D X, Hu J, et al. Monoterpenoid indole alkaloids from Alstonia mairei and their cytotoxicity[J]. J Asian Nat Prod Res, 2017, 19(6):550-556.
Zhao Y L, Shang J H, Pu S B, et al. Effect of total alkaloids from Alstonia scholaris on airway inflammation in rats[J].J Ethnopharmacol, 2016, 178:258-265.
Chua L S, Abdullah F I, Awang M A. Potential of natural bioactive C-glycosyl flavones for antidiabetic properties[M] //Bioactive Natural Products. Amsterdam:Elsevier,2020:241-261.
Li Y Q, Cai J, Cheng J X, et al. Alstomairines D–G:New monoterpenoid indole alkaloids with cytotoxic activity from Alstonia mairei[J]. J Mol Struct, 2022, 1253:132277.
Pierce C G, Uppuluri P, Tristan A R, et al. A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing[J]. Nat Protoc, 2008, 3(9):1494-1500.
Li X A, Zhang D Z, Onda M, et al. Ent-kauranoid diterpenes from Artemisia sacrorum[J]. J Nat Prod, 1990,53(3):657-661.
Chen W M, Yan Y P, Wang Y J, et al. Isolation and identification of three new alkaloids from the roots of Alstonia yunnanensis Diels Indiana, USA[J]. Acta Pharm Sin, 1985, 20(12):906-912.
TissandiéL, Viciana S, Brevard H, et al. Towards a complete characterisation of guaiacwood oil[J].Phytochemistry, 2018, 149:64-81.
Feng T, Li Y, Cai X H, et al. Monoterpenoid indole alkaloids from Alstonia yunnanensis[J]. J Nat Prod, 2009,72(10):1836-1841.
吴昊,宋京风,范堃,等.云南萝芙木根中吲哚生物碱及其抗菌活性[J].中草药, 2023, 54(4):1033-1042.
李文静,洪博,赵春杰.萝芙木化学成分的分离与鉴定[J].中国药房, 2013, 24(3):256-258.
Konda Y, Iguchi M, Harigaya Y, et al. Hancokinol, a novel triterpene, from Cynanchum hancokianum[J].Tetrahedron Lett, 1990, 31(37):5315-5318.
王妍,梁旭博,赵珍珠.泽漆全草中二萜类成分研究[J].中草药, 2022, 53(15):4625-4633.
2026年第57卷第7期
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doi: 10.7501/j.issn.0253-2670.2026.07.002
  • 接收时间:2026-01-27
  • 首发时间:2026-09-09
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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