中草药

茉莉酸甲酯处理对天麻中天麻素和巴利森苷E生物合成关键基因的影响

PDF下载

陈丽, 刘祥宇, 何元峰, 向贵生, 刀莉萍, 田孟华, 郝冰

中草药 | 药材与资源 2026,57(6): 2302-2315

收起

中草药 |药材与资源 2026 , 57 (6) : 2302 -2315

茉莉酸甲酯处理对天麻中天麻素和巴利森苷E生物合成关键基因的影响

全屏

陈丽, 刘祥宇, 何元峰, 向贵生, 刀莉萍, 田孟华, 郝冰

作者信息

作者简介:

陈丽: 陈丽,硕士研究生,研究方向为药用植物合成生物学。E-mail:2728809640@qq.com

Effects of methyl jasmonate treatment on key genes in biosynthesis of gastrodin and parishin E in Gastrodia elata

CHEN Li, LIU Xiangyu, HE Yuanfeng, XIANG Guisheng, DAO Liping, TIAN Menghua, HAO Bing

Affiliations

doi: 10.7501/j.issn.0253-2670.2026.06.024

文章导航

摘要

收起

目的基于茉莉酸甲酯（methyl jasmonate，MeJA）处理下的天麻Gastrodia elata比较转录组测序，筛选与天麻素及巴利森苷E化合物生物合成相关的关键酶基因。方法以箭麻和共生白头麻为实验材料，采用不同浓度（100～500 μmol/L）MeJA喷施处理不同时间（0～72 h），采用HPLC对天麻素和巴利森苷E的含量变化进行测定，对最佳MeJA处理条件下的样品进行转录组和代谢组联合分析，挖掘巴利森苷E生物合成途径的关键基因。结果在300 μmol/L MeJA处理48 h后，2个目标化合物含量均显著提升，其中巴利森苷E由初始的0.21 mg/g显著增至0.61 mg/g，提升2.90倍，故选定该处理条件进行转录组分析。测序共获得432.79 Gb Clean Data，检测到18 886个表达基因，含2 389个新基因。差异分析共筛选出5 704个差异基因，其中2 635个上调、3 069个下调。KEGG富集显示差异基因表达主要集中于苯丙素生物合成、植物信号转导等次生代谢相关通路。通过表达模式与化合物积累关联分析及同源比对，筛选出可能参与巴利森苷E生物合成途径中关键基因6条，糖基转移酶基因（GeUGT）、柠檬酰辅酶A合成酶基因（GeCCS）和酰基转移酶基因（GeBAHD）各2条。结论证实MeJA能有效调控天麻次生代谢，并筛选出与天麻素及巴利森苷E生物合成可能相关的候选基因（GeUGTs、GeCCSs、GeBAHDs），为完整解析这些关键活性成分的生物合成途径奠定了分子基础。

关键词

天麻 / 巴利森苷E / 茉莉酸甲酯 / 天麻素 / 组学分析 / 生物合成

Abstract

收起

Objective This study aimed to screen key enzyme genes associated with the biosynthesis of gastrodin and parishin E in Gastrodia elata. Through comparative transcriptome sequencing following methyl jasmonate (MeJA) treatment. Methods Using both the corm (MTE and the symbiotic tuber (IMTC) as experimental materials, different concentrations (100—500 μmol/L) of MeJA were sprayed for varying durations (0—72 h). The content changes of gastrodin and parishins E were determined by HPLC. Transcriptomic and metabolomic analyses were performed on samples treated under the optimal MeJA conditions to mine key genes in the parishin E biosynthetic pathway. Results After 48 h of treatment with 300 μmol/L MeJA, the contents of all six target compounds were significantly increased. Specifically, the content of parishin E increased from 0.21 mg/g to 0.61 mg/g, representing an approximately 2.90-fold enhancement. Transcriptome sequencing generated 432.79 Gb of Clean Data and identified 18 886 expressed genes, including 2 389 novel genes. Differential expression analysis revealed 5 704 differentially expressed genes, with 2 635 up-regulated and 3 069 down-regulated. KEGG enrichment analysis showed significant enrichment in phenylpropanoid biosynthesis, plant signal transduction, and other secondary metabolism-related pathways. Through integrated analysis of expression patterns and compound accumulation trends, along with homology comparison, we identified two glycosyltransferase genes GeUGT, two citrate-CoA synthetase genes GeCCS, and two acyltransferase genes GeBAHD potentially involved in parishin E biosynthesis. Conclusion This study demonstrates that MeJA effectively regulates secondary metabolism in G. elata and identifies candidate genes (GeUGTs, GeCCSs, GeBAHDs) potentially associated with the biosynthesis of gastrodin and parishin E, providing a molecular foundation for elucidating the complete biosynthetic pathways of these key active compounds.

Key words

Gastrodia elata Bl. / parishin E / methyl jasmonate / gastrodin / omics analysis / biosynthesis

引用本文

陈丽, 刘祥宇, 何元峰, 向贵生, 刀莉萍, 田孟华, 郝冰. 茉莉酸甲酯处理对天麻中天麻素和巴利森苷E生物合成关键基因的影响. 中草药, 2026 , 57 (6) : 2302 -2315 . DOI: 10.7501/j.issn.0253-2670.2026.06.024

CHEN Li, LIU Xiangyu, HE Yuanfeng, XIANG Guisheng, DAO Liping, TIAN Menghua, HAO Bing. Effects of methyl jasmonate treatment on key genes in biosynthesis of gastrodin and parishin E in Gastrodia elata[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (6) : 2302 -2315 . DOI: 10.7501/j.issn.0253-2670.2026.06.024

基金

收起

云南省科技厅基础研究专项 (202201AT070274)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

中国药典[S]. 一部. 2025: 89.
于潇, 王贵阳, 侯宇东, 等. 中药抗脑缺血再灌注损伤的作用及其机制的研究进展[J]. 中草药, 2021, 52(5): 1471-1484.
付亚轩, 孟宪钰, 李明超, 等. 天麻抗抑郁药效物质及其作用机制研究进展[J]. 中草药, 2020, 51(21): 5622-5630.
Zhang Z Y, Li X D, Zhang Y C, et al. Ecological factors impacting genetic characteristics and metabolite accumulations of Gastrodia elata[J]. Chin Herb Med, 2025, 17(3): 562-574.
Su Z H, Yang Y G, Chen S Z, et al. The processing methods, phytochemistry and pharmacology of Gastrodia elata Bl.: A comprehensive review[J]. J Ethnopharmacol, 2023, 314: 116467.
Zhou H B, Lu S Z, Yu Z S, et al. Mechanisms for the biological activity of Gastrodia elata Blume and its constituents: A comprehensive review on sedative-hypnotic, and antidepressant properties[J]. Phytomedicine, 2024, 123: 155251.
于涵, 张俊, 陈碧清, 等. 天麻化学成分分类及其药理作用研究进展[J]. 中草药, 2022, 53(17): 5553-5564.
徐德宏, 崔培梧, 罗怀浩, 等. 天麻素生物合成的研究进展[J]. 中草药, 2020, 51(22): 5877-5883.
张志龙, 郜玉钢, 臧埔, 等. 天麻素、对羟基苯甲醇对中枢神经系统作用机制研究进展[J]. 中国中药杂志, 2020, 45(2): 312-320.
陈善军, 王欢, 胡凯庆, 等. 基于斑马鱼模型和代谢组学技术筛选天麻中潜在抗癫痫活性成分[J]. 山东科学, 2024, 37(3): 1-9.
Liu P, Zhao Z H, Zhang H L, et al. A comprehensive pharmacology study reveals the molecular mechanisms underlying the antidepressant effects of Gastrodiae Rhizoma [J]. Phytomedicine, 2025, 142: 156761.
Wang L L, Hu M, Fu Y, et al. Ginger processing remodels the metabolome profile of Gastrodia elata and potentiates its neuroprotective effects[J]. Phytomedicine, 2025, 148: 157469.
张菊, 宋娜丽, 马克坚. 天麻中巴利森苷类成分药理作用、体内过程研究进展[J]. 中成药, 2022, 44(7): 2223-2229.
陈贵生. 天麻素药理作用研究进展[J]. 中国药物经济学, 2015, 10(S1): 281-283.
黄娟. 天麻素与天麻苷元肠吸收机制研究[D]. 广州: 南方医科大学, 2012.
谢淼, 邵明莎, 翟庆超, 等. 天麻中巴利森苷类成分研究进展[J]. 广东化工, 2016, 43(22): 93-95.
单鸣秋, 张丽, 于生, 等. HPLC-MS法同时测定天麻饮片中8种活性成分[J]. 中草药, 2015, 46(14): 2087-2091.
Tang C L, Wang L, Liu X X, et al. Comparative pharmacokinetics of gastrodin in rats after intragastric administration of free gastrodin, parishin and Gastrodia elata extract[J]. J Ethnopharmacol, 2015, 176: 49-54.
Fu Y, Xu Q Q, Zhang J Q, et al. Identifying the quality markers and optimizing the processing of Gastrodiae Rhizoma to treat brain diseases[J]. Front Pharmacol, 2024, 15: 1396825.
Chen R B, Li Q, Tan H X, et al. Gene-to-metabolite network for biosynthesis of lignans in MeJA-elicited Isatis indigotica hairy root cultures[J]. Front Plant Sci, 2015, 6: 952.
樊荣辉, 孔兰, 林榕燕, 等. 基于转录组测序的建兰花香相关基因挖掘及表达分析[J]. 东南园艺, 2022, 10(6): 401-407.
Xu Q, Wang S T, Hong H Z, et al. Transcriptomic profiling of the flower scent biosynthesis pathway of Cymbidium faberi Rolfe and functional characterization of its jasmonic acid carboxyl methyltransferase gene[J]. BMC Genomics, 2019, 20(1): 125.
林江波, 邹晖, 王伟英, 等. 铁皮石斛DoLIS基因克隆与茉莉酸甲酯诱导表达分析[J]. 福建农业学报, 2020, 35(10): 1071-1077.
罗才林. 水杨酸和茉莉酸甲酯胁迫下白及响应与苯丙氨酸解氨酶基因的克隆[D]. 遵义: 遵义医科大学, 2019.
Bolger A M, Lohse M, Usadel B. Trimmomatic: A flexible trimmer for Illumina sequence data[J]. Bioinformatics, 2014, 30(15): 2114-2120.
Deng Z L, Münch P C, Mreches R, et al. Rapid and accurate identification of ribosomal RNA sequences via deep learning[J]. Nucleic Acids Res, 2022, 50(10): e60.
Xu Y X, Lei Y T, Su Z X, et al. A chromosome-scale Gastrodia elata genome and large-scale comparative genomic analysis indicate convergent evolution by gene loss in mycoheterotrophic and parasitic plants[J]. Plant J, 2021, 108(6): 1609-1623.
Chen Q L, Steinhauer L, Hammerlindl J, et al. Biosynthesis of phytosterol esters: Identification of a sterol o-acyltransferase in Arabidopsis[J]. Plant Physiol, 2007, 145(3): 974-984.
Chen X L, Fang D M, Xu Y X, et al. Balanophora genomes display massively convergent evolution with other extreme holoparasites and provide novel insights into parasite-host interactions[J]. Nat Plants, 2023, 9(10): 1627-1642.
刘云霞, 狄永国, 仇全雷, 等. 基于转录组测序初步揭示天麻生长代谢的分子机制[J]. 中草药, 2021, 52(3): 827-837.
Aoshima M, Ishii M, Igarashi Y. A novel enzyme, citryl-CoA synthetase, catalysing the first step of the citrate cleavage reaction in Hydrogenobacter thermophilus TK-6[J]. Mol Microbiol, 2004, 52(3): 751-761.
Yang Y H, Xi D Y, Wu Y N, et al. Complete biosynthesis of the phenylethanoid glycoside verbascoside[J]. Plant Commun, 2023, 4(4): 100592.
刘益宏, 聂江力, 裴毅. 诱导子对药用植物次生代谢产物积累的作用综述[J]. 天津农林科技, 2024(5): 39-42.
Jiao C Y, Song C, Zheng S Y, et al. Metabolic profiling of Dendrobium officinale in response to precursors and methyl jasmonate[J]. Int J Mol Sci, 2018, 19(3): 728.
Zhang W H, Zhang J Z, Fan Y D, et al. RNA sequencing analysis reveals PgbHLH28 as the key regulator in response to methyl jasmonate-induced saponin accumulation in Platycodon grandiflorus[J]. Hortic Res, 2024, 11(5): uhae058.
Guo F Y, Zhou H, Yan F F, et al. A comprehensive multiomics approach illuminates the biosynthetic mechanism of scoparone in Artemisia capillaris[J]. Plant Biotechnol J, 2025. DOI:10.1111/pbi.70398.
Yin H, Hu T D, Zhuang Y B, et al. Metabolic engineering of Saccharomyces cerevisiae for high-level production of gastrodin from glucose[J]. Microb Cell Fact, 2020, 19(1): 218.
吴克红, 谢颖, 曹皇亮, 等. 天麻中巴利森类物质的化学、药理和体内外代谢研究进展[J]. 中草药, 2025, 56(13): 4841-4851.
Park M K, Choi B Y, Kho A R, et al. L-theanine ameliorates traumatic-brain-injury-induced hippocampal neuronal death in rats[J]. Phytomedicine, 2025, 139: 156457.

2026年第57卷第6期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.7501/j.issn.0253-2670.2026.06.024

接收时间：2025-11-02
首发时间：2026-09-09

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2025-11-02

基金

作者信息

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.06.024

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT