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反柄紫芝化学成分及其生物活性研究

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中草药 | 化学成分 2026,57(6): 2009-2015

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中草药 |化学成分 2026 , 57 (6) : 2009 -2015

反柄紫芝化学成分及其生物活性研究

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叶家文: 叶家文,硕士研究生,中药学专业。E-mail:zdtsyejiawen@stu.cdutcm.edu.cn

Chemical constituents isolated from Ganoderma cochlear and their biological activities

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doi: 10.7501/j.issn.0253-2670.2026.06.001

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目的研究多孔菌科真菌反柄紫芝Ganoderma cochlear甲醇提取物的化学成分，并初步评价其体外抗动脉粥样硬化活性。方法采用D-101大孔吸附树脂、硅胶柱色谱、反向C₁₈柱色谱、制备薄层色谱及半制备高效液相色谱等技术对提取物进行分离纯化，并通过高分辨质谱、红外光谱、核磁共振等波谱手段鉴定化合物结构，再运用计算NMR和计算ECD确定新化合物的绝对构型；采用氧化低密度脂蛋白（oxidized low-density lipoprotein，ox-LDL）诱导的巨噬细胞脂质沉积模型评估化合物体外抗动脉粥样硬化活性。结果从反柄紫芝中分离鉴定了2个新颖的化合物，分别鉴定为7,10-环氧-3,4-裂环-9(10→19)迁-25,26,27-三降羊毛脂甾烷-4,11-二氧-8-烯-3,24-二羧酸甲酯（1）和6-羟基-2-(4-甲基戊-3-烯-1-基)-4H-色烯-4-酮（2）。在ox-LDL诱导的巨噬细胞模型中，化合物1和2在40  μmol/L浓度下未表现出明显的抗脂质沉积活性。结论成功分离并鉴定了2个结构新颖的化合物，分别命名为反柄紫芝萜J（1）和反柄紫芝萜K（2）；其中化合物1代表首个拥有5β-H的灵芝三萜，丰富了灵芝的化学多样性。化合物1和2在初步活性评价中未显示显著的体外抗动脉粥样硬化作用。

关键词

反柄紫芝 / 三萜 / 杂萜 / 反柄紫芝萜J / 反柄紫芝萜K / 灵芝三萜 / 脂质沉积

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Objective To investigate the chemical constituents from the methanol extract of the polyporaceae fungus Ganoderma cochlear and preliminarily evaluate their anti-atherosclerotic activity in vitro. Methods The extract was systematically isolated and purified by D-101 macroporous adsorption resin, silica gel column chromatography, reversed-phase C₁₈ column chromatography, preparative thin layer chromatography and semi-preparative HPLC. The structures of the isolated compounds were elucidated by HR-ESI-MS, IR, and NMR. Meanwhile, the absolute configurations of the new compounds were determined by NMR and ECD calculation. The in vitro anti-atherosclerotic activities of the isolates were assessed using an oxidized low-density lipoprotein (ox-LDL)-induced macrophage lipid deposition model. Results Two novel compounds were isolated and identified from G. cochlear, designated as 7,10-epoxy-3,4-seco-9 (10→19) abeo-25,26,27-trinorlanosta-4,11-dioxo-8-en-3,24-dimethyl ester (1) and 6-hydroxy-2-(4-methylpent-3-en-1-yl)-4H-chromen-4-one (2). In the ox-LDL-induced macrophage model, compounds 1 and 2 did not exhibit significant anti-lipid deposition activity at 40 μmol/L. Conclusion This study successfully isolated and identified two new compounds, and named as ganodeconoid J (1) and ganodeconoid K (2). Compound 1 represents the first Ganoderma triterpenoid possessing a 5β-H, enriching the chemical diversity of Ganoderma fungi. Compounds 1 and 2 did not show significant in vitro anti-atherosclerotic activity.

Key words

Ganoderma cochlear (Blume & T. Nees) Bres. / triterpenoids / meroterpenoids / ganodeconoid J / ganodeconoid K / Ganoderma triterpenoid / lipid deposition

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叶家文, 褚天骄, 熊利, 王欣叶, 苏海国, 熊亮. 反柄紫芝化学成分及其生物活性研究. 中草药, 2026 , 57 (6) : 2009 -2015 . DOI: 10.7501/j.issn.0253-2670.2026.06.001

YE Jiawen, CHU Tianjiao, XIONG Li, WANG Xinye, SU Haiguo, XIONG Liang. Chemical constituents isolated from Ganoderma cochlear and their biological activities[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (6) : 2009 -2015 . DOI: 10.7501/j.issn.0253-2670.2026.06.001

基金

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国家自然科学基金项目 (82204226)

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2026年第57卷第6期

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doi: 10.7501/j.issn.0253-2670.2026.06.001

接收时间：2025-12-09
首发时间：2026-09-09

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https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.06.001

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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