Article(id=1304414870779220037, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414858296971266, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.06.006, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1762704000000, receivedDateStr=2025-11-10, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788926340049, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788926340049, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788926340049, creator=13701087609, updateTime=1788926340049, updator=13701087609, issue=Issue{id=1304414858296971266, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='6', pageStart='2009', pageEnd='2444', issueExtLink='null', onlineDate='null', pubDate='1774627200000', pubDateStr='2026-03-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788926337074, creator='13701087609', updateTime=1788926665348, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304416235240841997, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414858296971266, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304416235240841998, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414858296971266, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=2060, endPage=2074, ext={EN=ArticleExt(id=1304414871190261831, articleId=1304414870779220037, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Preparation and characterization of polyphyllin VII targeted nanoparticles based on metal-organic framework materials IRMOF-10 and preliminary evaluation of anti-triple negative breast cancer efficacy, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective To prepare hyaluronic acid-modified polyphyllin VII-loaded metal-organic framework material IRMOF-10 targeted nanoparticles (HA@IRMOF-10@PPVII), systematically evaluate their physicochemical properties, safety and in vitro anti-triple negative breast cancer (TNBC) activity. Methods Metal-organic framework material IRMOF-10 was synthesized by triethylamine method. PPVII was encapsulated by the heterotopic encapsulation method, and the surface of the drug-loaded nanoparticles was modified with hyaluronic acid to construct the targeted nanoparticles HA@IRMOF-10@PPVII. The drug loading was determined by HPLC. The morphology, structure and thermal stability of nanoparticles were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TG) and BET specific surface area method. Its blood safety was evaluated by hemolysis test, and its cell targeting was verified by laser confocal microscopy. CCK-8, DAPI staining, Annexin V/PI double staining, migration assay, clone formation assay, cell uptake, reactive oxygen species (ROS) detection, mitochondrial membrane potential (MMP) detection and other methods were used to verify its inhibitory effect on TNBC 4T1 cells. Results The drug loading capacity of HA@IRMOF-10@PPVII was (39.56% ± 1.78)% (n = 3). HA@IRMOF-10@PPVII had good biosafety, which could significantly reduce the hemolysis risk of PPVII. This nanoparticle can effectively improve the uptake of PPVII in 4T1 cells, and significantly inhibits cell migration and clone formation. Its anti-tumor mechanism is closely related to inducing an increase in intracellular ROS levels, a decrease in MMP triggering apoptosis, and inhibiting the migration and cloning of 4T1 cells. Conclusion The HA@IRMOF-10@PPVII nanoparticles were successfully prepared, which exhibit excellent targeting ability and anti-TNBC activity. They can significantly enhance the efficacy of PPVII, providing experimental evidence for the development of PPVII-based anti-tumor formulations., authors=LIN Shuting, YIN Dongge, ZHU Rongyue, CHEN Hongyue, JING Xiaohong, WANG Wenqi, CHANG Rongrong, SUN Yufei, DENG Xixi, DONG Xiaoxu, QV Changhai, NI Jian, YIN Xingbin, authorsList=LIN Shuting, YIN Dongge, ZHU Rongyue, CHEN Hongyue, JING Xiaohong, WANG Wenqi, CHANG Rongrong, SUN Yufei, DENG Xixi, DONG Xiaoxu, QV Changhai, NI Jian, YIN Xingbin, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304414871068627014, articleId=1304414870779220037, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=基于金属有机骨架材料IRMOF-10的重楼皂苷VII靶向纳米粒制备、表征及抗三阴性乳腺癌药效评价, columnId=1304140189132149234, journalTitle=中草药, columnName=药剂与工艺, runingTitle=null, highlight=null, articleAbstract=目的 构建透明质酸（hyaluronic acid，HA）修饰的载重楼皂苷VII（polyphyllin VII，PPVII）金属有机骨架材料IRMOF-10靶向纳米粒HA@IRMOF-10@PPVII，并系统评价其理化特性、安全性和体外抗三阴性乳腺癌（triple negative breast cancer，TNBC）活性。方法 采用三乙胺法制备金属有机骨架材料IRMOF-10，通过异位包封法包载PPVII，并利用透明质酸对载药纳米粒表面进行修饰，构建靶向纳米粒HA@IRMOF-10@PPVII；采用HPLC法测定其载药量，运用扫描电子显微镜（scanning electron microscopy，SEM）、傅里叶变换红外光谱（Fourier transform infrared spectroscopy，FTIR）分析、热重分析（thermogravimetric analysis，TG）及BET比表面积法等分析手段表征纳米粒的形貌、结构及热稳定性；通过溶血实验评估其血液安全性，采用激光共聚焦显微镜验证其靶向能力，通过CCK-8法、DAPI染色、Annexin V/PI双染、迁移实验、克隆形成实验，以及细胞摄取、活性氧（reactive oxygen species，ROS）检测、线粒体膜电位（mitochondrial membrane potential，MMP）检测等方法，综合评价纳米粒对TNBC 4T1细胞的抑制作用。结果 所制备的HA@IRMOF-10@PPVII载药量为 （39.56±1.78）%（n＝3），具备良好的生物安全性，可显著降低PPVII的溶血风险；该纳米粒能够有效增强PPVII在4T1细胞中的摄取，并显著抑制细胞迁移与克隆形成；其抗肿瘤机制与诱导细胞内ROS水平升高、MMP下降触发细胞凋亡、抑制4T1细胞的迁移与克隆密切相关。结论 HA@IRMOF-10@PPVII纳米粒制备成功，具有良好的靶向性与抗TNBC活性，能够显著增强PPVII的药效，为基于PPVII的抗肿瘤制剂研发提供了实验依据。, authors=林舒婷1, 尹东阁1, 朱荣玥2, 陈红月1, 荆霄鸿1, 王雯琪1, 常榕蓉1, 孙宇菲1, 邓兮玺1, 董晓旭1, 曲昌海1, 倪健1, 尹兴斌1, authorsList=林舒婷, 尹东阁, 朱荣玥, 陈红月, 荆霄鸿, 王雯琪, 常榕蓉, 孙宇菲, 邓兮玺, 董晓旭, 曲昌海, 倪健, 尹兴斌, authorCompany=1 北京中医药大学中药学院, 北京 102488; 2 吉林省药品审评中心, 吉林 长春 130000, correspAuthors=null, authorNote=林舒婷: 林舒婷,硕士研究生,从事中药新剂型与新技术的研究。E-mail:20240935230@bucm.edu.cn, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=sAY5xAg1sqxQR1Dv8gFAnA==, pdfFileSize=2132609, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=北京市自然科学基金项目 (7262198); 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Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2024, 74(3): 229-263. Siegel R L, Kratzer T B, Giaquinto A N, et al. Cancer statistics, 2025[J]. CA A Cancer J Clin, 2025, 75(1): 10-45. Yin L, Duan J J, Bian X W, et al. Triple-negative breast cancer molecular subtyping and treatment progress[J]. Breast Cancer Res, 2020, 22(1): 61. Chapdelaine A G, Sun G Q. Challenges and opportunities in developing targeted therapies for triple negative breast cancer[J]. Biomolecules, 2023, 13(8): 1207. 杨秋莉, 王学芬, 张向农. 古代中医对乳腺癌的认识[J]. 中国中医基础医学杂志, 2010, 16(5): 437-439. 王春晖, 裴晓华, 孙艳丽. 乳腺癌的古今中医认识及治疗研究概况[J]. 世界中西医结合杂志, 2016, 11(9): 1323-1325. 刘明明, 丁舸, 赵益, 等. 基于数据挖掘分析熊墨年辨治乳腺癌组方用药规律[J]. 新中医, 2021, 53(7): 104-110. 王桂彬, 谭世珺, 宋雪, 等. 中医临床“六辨”思维视域下乳腺癌全周期管理与辨治[J]. 北京中医药大学学报, 2025, 48(12): 1629-1635. 董晶, 汪秋颖, 皮佩仙, 等. 七叶一枝花抗癌临床应用及作用机制研究进展[J]. 中国民族民间医药, 2024, 33(13): 42-48. 李世香, 梁智蓉, 杨生丽, 等. 一例巨块型溃破乳腺癌中西医整合治疗分享[J]. 基层中医药, 2025, 4(8): 63-68. Zhang C, Jia X J, Bao J L, et al. Polyphyllin VII induces apoptosis in HepG2 cells through ROS-mediated mitochondrial dysfunction and MAPK pathways[J]. BMC Complement Altern Med, 2016, 16: 58. Lin Z F, Liu Y T, Li F Y, et al. Anti-lung cancer effects of polyphyllin VI and VII potentially correlate with apoptosis in vitro and in vivo[J]. Phytother Res, 2015, 29(10): 1568-1576. Wang H Q, Xiao X J, Li Z Z, et al. Polyphyllin VII, a novel moesin inhibitor, suppresses cell growth and overcomes bortezomib resistance in multiple myeloma[J]. Cancer Lett, 2022, 537: 215647. Hamouda A O, Sarg N H, Ibrahim S I, et al. Structure-driven mechanisms and synergistic approaches of polyphyllin VII in breast cancer therapy[J]. Arch Der Pharm, 2025, 358(7): e70064. Li Y H, Sun Y, Tang T L, et al. Paris saponin VII reverses chemoresistance in breast MCF-7/ADR cells[J]. J Ethnopharmacol, 2019, 232: 47-54. Zhang X, Sun Y, Cheng Y, et al. Biopharmaceutics classification evaluation for Paris saponin VII[J]. Chin J Nat Med, 2020, 18(9): 714-720. Wang W P, Liu Y, Sun M Y, et al. Hepatocellular toxicity of Paris saponins I, II, VI and VII on two kinds of hepatocytes-HL-7702 and HepaRG cells, and the underlying mechanisms[J]. Cells, 2019, 8(7): 690. 高飞燕, 刘鑫龙, 彭珊, 等. 鼠李糖类似物介导的脂质体递药系统及其靶向抗胰腺癌的研究[J]. 药学学报, 2024, 59(4): 1067-1078. Liu Y, An T Q, Que Z J, et al. Inhalable herbal nano-liposomes co-delivering astragaloside IV and polyphyllin VII reprogram immunosuppressive microenvironment to overcome anti-PD-1 resistance in NSCLC[J]. Biol Proced Online, 2025, 27(1): 43. Bai S B, Sun Y, Cheng Y, et al. MCP mediated active targeting calcium phosphate hybrid nanoparticles for the treatment of orthotopic drug-resistant colon cancer[J]. J Nanobiotechnology, 2021, 19(1): 367. 蔡梦如, 姚宇, 尹东阁, 等. 金属有机骨架材料在中药研究的应用进展[J]. 中草药, 2021, 52(16): 5028-5038. Wang K X, Cai M R, Yin D G, et al. Functional metal-organic framework nanoparticles loaded with polyphyllin I for targeted tumor therapy[J]. J Sci Adv Mater Devices, 2023, 8(2): 100548. Filiz A, Bayazit Ş S, Baris Barlas F. Metal-organic frameworks (MOFs) in drug delivery: Emerging trends, functional enhancements, and biocompatibility challenges[J]. J Drug Deliv Sci Technol, 2025, 112: 107284. Cai M R, Yang Y Q, Kong J H, et al. Nanoscale metal-organic frameworks as a versatile platform for synergistic combination tumor therapy[J]. J Nanobiotechnology, 2025, 23(1): 601. Borycz J, Tiana D, Haldoupis E, et al. CO2 adsorption in M-IRMOF-10(M = Mg, Ca, Fe, Cu, Zn, Ge, Sr, Cd, Sn, Ba)[J]. J Phys Chem C, 2016, 120(23): 12819-12830. Liu Z W, Zhang K, Wu Y, et al. New functionalized IRMOF-10 with strong affinity for methanol: A simulation study[J]. Appl Surf Sci, 2018, 440: 351-358. Cai M R, Liang W L, Wang K X, et al. Aperture modulation of isoreticular metal organic frameworks for targeted antitumor drug delivery[J]. ACS Appl Mater Interfaces, 2022, 14(32): 36366-36378. Yin D G, Hu X L, Cai M R, et al. Preparation, characterization, and invitro release of curcumin-loaded IRMOF-10 nanoparticles and investigation of their pro-apoptotic effects on human hepatoma HepG2 cells[J]. Molecules, 2022, 27(12): 3940. Liu X, Taftaf R, Kawaguchi M, etal. Homophilic CD44 interactions mediate tumor cell aggregation and polyclonal metastasis in patient-derived breast cancer models[J]. Cancer Discov, 2019, 9(1): 96-113. Xu H X, Niu M K, Yuan X, et al. CD44 as a tumor biomarker and therapeutic target[J]. Exp Hematol Oncol, 2020, 9(1): 36. Guo Q, Yang C X, Gao F. The state of CD44 activation in cancer progression and therapeutic targeting[J]. FEBS J, 2022, 289(24): 7970-7986. Peira E, Sapino S, Chirio D, et al. Formulation and comparative characterization of SLNs and NLCs for targeted co-delivery of paclitaxel and hydroxytyrosol carboxylic acid esters against triple-negative breast cancer[J]. Pharmaceutics, 2025, 17(9): 1208. Soares F A, Salinas B, Reis S, et al. Hyaluronic acid-engineered milk extracellular vesicles to target triple negative breast cancer through CD44[J]. Pharm Biol, 2025, 63(1): 411-427. Wang J, Wang J Y, Zhang J, et al. Bimetallic chitosan/hyaluronic acid nanoparticles self-amplify ferroptosis/cuproptosis in triple-negative breast cancer[J]. Int J Biol Macromol, 2025, 308(Pt 4): 142535. Sharma R, Yadav V, Katari O, et al. Hyaluronic acid functionalized liposomes for co-delivery of paclitaxel and ursolic acid for enhanced efficacy against triple negative breast cancer[J]. J Drug Deliv Sci Technol, 2025, 104: 106451. Cai M R, Yao Y, Yin D G, et al. Enhanced lysosomal escape of cell penetrating peptide-functionalized metal-organic frameworks for co-delivery of survivin siRNA and oridonin[J]. J Colloid Interface Sci, 2023, 646: 370-380. Zhao L, Liu Z Y, Deng X Y, et al. Polyphyllin VII induces mitochondrial apoptosis by regulating the PP2A/Akt/ DRP1 signaling axis in human ovarian cancer[J]. Oncol Rep, 2021, 45(2): 513-522. Ahmad B, Rehman S U, Azizullah A, et al. Molecular mechanisms of anticancer activities of polyphyllin VII[J]. Chem Biol Drug Des, 2021, 97(4): 914-929. Xiang Y C, Peng P, Liu X W, et al. Paris saponin VII, a Hippo pathway activator, induces autophagy and exhibits therapeutic potential against human breast cancer cells[J]. Acta Pharmacol Sin, 2022, 43(6): 1568-1580. Zhang Y B, Wei S J, Zhang Q X, et al. Paris saponin VII inhibits triple-negative breast cancer by targeting the MEK/ERK/STMN1 signaling axis[J]. Phytomedicine, 2024, 130: 155746.)
Preparation and characterization of polyphyllin VII targeted nanoparticles based on metal-organic framework materials IRMOF-10 and preliminary evaluation of anti-triple negative breast cancer efficacy
LIN Shuting, YIN Dongge, ZHU Rongyue, CHEN Hongyue, JING Xiaohong, WANG Wenqi, CHANG Rongrong, SUN Yufei, DENG Xixi, DONG Xiaoxu, QV Changhai, NI Jian, YIN Xingbin
Objective To prepare hyaluronic acid-modified polyphyllin VII-loaded metal-organic framework material IRMOF-10 targeted nanoparticles (HA@IRMOF-10@PPVII), systematically evaluate their physicochemical properties, safety and in vitro anti-triple negative breast cancer (TNBC) activity. Methods Metal-organic framework material IRMOF-10 was synthesized by triethylamine method. PPVII was encapsulated by the heterotopic encapsulation method, and the surface of the drug-loaded nanoparticles was modified with hyaluronic acid to construct the targeted nanoparticles HA@IRMOF-10@PPVII. The drug loading was determined by HPLC. The morphology, structure and thermal stability of nanoparticles were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TG) and BET specific surface area method. Its blood safety was evaluated by hemolysis test, and its cell targeting was verified by laser confocal microscopy. CCK-8, DAPI staining, Annexin V/PI double staining, migration assay, clone formation assay, cell uptake, reactive oxygen species (ROS) detection, mitochondrial membrane potential (MMP) detection and other methods were used to verify its inhibitory effect on TNBC 4T1 cells. Results The drug loading capacity of HA@IRMOF-10@PPVII was (39.56% ± 1.78)% (n = 3). HA@IRMOF-10@PPVII had good biosafety, which could significantly reduce the hemolysis risk of PPVII. This nanoparticle can effectively improve the uptake of PPVII in 4T1 cells, and significantly inhibits cell migration and clone formation. Its anti-tumor mechanism is closely related to inducing an increase in intracellular ROS levels, a decrease in MMP triggering apoptosis, and inhibiting the migration and cloning of 4T1 cells. Conclusion The HA@IRMOF-10@PPVII nanoparticles were successfully prepared, which exhibit excellent targeting ability and anti-TNBC activity. They can significantly enhance the efficacy of PPVII, providing experimental evidence for the development of PPVII-based anti-tumor formulations.
Key words
polyphyllin VII
/
metal organic framework materials
/
IRMOF-10
/
anti-tumor
/
triple negative breast cancer
LIN Shuting, YIN Dongge, ZHU Rongyue, CHEN Hongyue, JING Xiaohong, WANG Wenqi, CHANG Rongrong, SUN Yufei, DENG Xixi, DONG Xiaoxu, QV Changhai, NI Jian, YIN Xingbin.
Preparation and characterization of polyphyllin VII targeted nanoparticles based on metal-organic framework materials IRMOF-10 and preliminary evaluation of anti-triple negative breast cancer efficacy[J].
Chinese Traditional and Herbal Drugs,
2026
, 57
(6)
: 2060
-2074
.
DOI: 10.7501/j.issn.0253-2670.2026.06.006
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收起
北京市自然科学基金项目 (7262198); 国家自然科学基金项目 (81703715)
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Bray F, Laversanne M, Sung H, et al. Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2024, 74(3): 229-263. Siegel R L, Kratzer T B, Giaquinto A N, et al. Cancer statistics, 2025[J]. CA A Cancer J Clin, 2025, 75(1): 10-45. Yin L, Duan J J, Bian X W, et al. Triple-negative breast cancer molecular subtyping and treatment progress[J]. Breast Cancer Res, 2020, 22(1): 61. Chapdelaine A G, Sun G Q. Challenges and opportunities in developing targeted therapies for triple negative breast cancer[J]. Biomolecules, 2023, 13(8): 1207. 杨秋莉, 王学芬, 张向农. 古代中医对乳腺癌的认识[J]. 中国中医基础医学杂志, 2010, 16(5): 437-439. 王春晖, 裴晓华, 孙艳丽. 乳腺癌的古今中医认识及治疗研究概况[J]. 世界中西医结合杂志, 2016, 11(9): 1323-1325. 刘明明, 丁舸, 赵益, 等. 基于数据挖掘分析熊墨年辨治乳腺癌组方用药规律[J]. 新中医, 2021, 53(7): 104-110. 王桂彬, 谭世珺, 宋雪, 等. 中医临床“六辨”思维视域下乳腺癌全周期管理与辨治[J]. 北京中医药大学学报, 2025, 48(12): 1629-1635. 董晶, 汪秋颖, 皮佩仙, 等. 七叶一枝花抗癌临床应用及作用机制研究进展[J]. 中国民族民间医药, 2024, 33(13): 42-48. 李世香, 梁智蓉, 杨生丽, 等. 一例巨块型溃破乳腺癌中西医整合治疗分享[J]. 基层中医药, 2025, 4(8): 63-68. Zhang C, Jia X J, Bao J L, et al. Polyphyllin VII induces apoptosis in HepG2 cells through ROS-mediated mitochondrial dysfunction and MAPK pathways[J]. BMC Complement Altern Med, 2016, 16: 58. Lin Z F, Liu Y T, Li F Y, et al. Anti-lung cancer effects of polyphyllin VI and VII potentially correlate with apoptosis in vitro and in vivo[J]. Phytother Res, 2015, 29(10): 1568-1576. Wang H Q, Xiao X J, Li Z Z, et al. Polyphyllin VII, a novel moesin inhibitor, suppresses cell growth and overcomes bortezomib resistance in multiple myeloma[J]. Cancer Lett, 2022, 537: 215647. Hamouda A O, Sarg N H, Ibrahim S I, et al. Structure-driven mechanisms and synergistic approaches of polyphyllin VII in breast cancer therapy[J]. Arch Der Pharm, 2025, 358(7): e70064. Li Y H, Sun Y, Tang T L, et al. Paris saponin VII reverses chemoresistance in breast MCF-7/ADR cells[J]. J Ethnopharmacol, 2019, 232: 47-54. Zhang X, Sun Y, Cheng Y, et al. Biopharmaceutics classification evaluation for Paris saponin VII[J]. Chin J Nat Med, 2020, 18(9): 714-720. Wang W P, Liu Y, Sun M Y, et al. Hepatocellular toxicity of Paris saponins I, II, VI and VII on two kinds of hepatocytes-HL-7702 and HepaRG cells, and the underlying mechanisms[J]. Cells, 2019, 8(7): 690. 高飞燕, 刘鑫龙, 彭珊, 等. 鼠李糖类似物介导的脂质体递药系统及其靶向抗胰腺癌的研究[J]. 药学学报, 2024, 59(4): 1067-1078. Liu Y, An T Q, Que Z J, et al. Inhalable herbal nano-liposomes co-delivering astragaloside IV and polyphyllin VII reprogram immunosuppressive microenvironment to overcome anti-PD-1 resistance in NSCLC[J]. Biol Proced Online, 2025, 27(1): 43. Bai S B, Sun Y, Cheng Y, et al. MCP mediated active targeting calcium phosphate hybrid nanoparticles for the treatment of orthotopic drug-resistant colon cancer[J]. J Nanobiotechnology, 2021, 19(1): 367. 蔡梦如, 姚宇, 尹东阁, 等. 金属有机骨架材料在中药研究的应用进展[J]. 中草药, 2021, 52(16): 5028-5038. Wang K X, Cai M R, Yin D G, et al. Functional metal-organic framework nanoparticles loaded with polyphyllin I for targeted tumor therapy[J]. J Sci Adv Mater Devices, 2023, 8(2): 100548. Filiz A, Bayazit Ş S, Baris Barlas F. Metal-organic frameworks (MOFs) in drug delivery: Emerging trends, functional enhancements, and biocompatibility challenges[J]. J Drug Deliv Sci Technol, 2025, 112: 107284. Cai M R, Yang Y Q, Kong J H, et al. Nanoscale metal-organic frameworks as a versatile platform for synergistic combination tumor therapy[J]. J Nanobiotechnology, 2025, 23(1): 601. Borycz J, Tiana D, Haldoupis E, et al. CO2 adsorption in M-IRMOF-10(M = Mg, Ca, Fe, Cu, Zn, Ge, Sr, Cd, Sn, Ba)[J]. J Phys Chem C, 2016, 120(23): 12819-12830. Liu Z W, Zhang K, Wu Y, et al. New functionalized IRMOF-10 with strong affinity for methanol: A simulation study[J]. Appl Surf Sci, 2018, 440: 351-358. Cai M R, Liang W L, Wang K X, et al. Aperture modulation of isoreticular metal organic frameworks for targeted antitumor drug delivery[J]. ACS Appl Mater Interfaces, 2022, 14(32): 36366-36378. Yin D G, Hu X L, Cai M R, et al. Preparation, characterization, and invitro release of curcumin-loaded IRMOF-10 nanoparticles and investigation of their pro-apoptotic effects on human hepatoma HepG2 cells[J]. Molecules, 2022, 27(12): 3940. Liu X, Taftaf R, Kawaguchi M, etal. Homophilic CD44 interactions mediate tumor cell aggregation and polyclonal metastasis in patient-derived breast cancer models[J]. Cancer Discov, 2019, 9(1): 96-113. Xu H X, Niu M K, Yuan X, et al. CD44 as a tumor biomarker and therapeutic target[J]. Exp Hematol Oncol, 2020, 9(1): 36. Guo Q, Yang C X, Gao F. The state of CD44 activation in cancer progression and therapeutic targeting[J]. FEBS J, 2022, 289(24): 7970-7986. Peira E, Sapino S, Chirio D, et al. Formulation and comparative characterization of SLNs and NLCs for targeted co-delivery of paclitaxel and hydroxytyrosol carboxylic acid esters against triple-negative breast cancer[J]. Pharmaceutics, 2025, 17(9): 1208. Soares F A, Salinas B, Reis S, et al. Hyaluronic acid-engineered milk extracellular vesicles to target triple negative breast cancer through CD44[J]. Pharm Biol, 2025, 63(1): 411-427. Wang J, Wang J Y, Zhang J, et al. Bimetallic chitosan/hyaluronic acid nanoparticles self-amplify ferroptosis/cuproptosis in triple-negative breast cancer[J]. Int J Biol Macromol, 2025, 308(Pt 4): 142535. Sharma R, Yadav V, Katari O, et al. Hyaluronic acid functionalized liposomes for co-delivery of paclitaxel and ursolic acid for enhanced efficacy against triple negative breast cancer[J]. J Drug Deliv Sci Technol, 2025, 104: 106451. Cai M R, Yao Y, Yin D G, et al. Enhanced lysosomal escape of cell penetrating peptide-functionalized metal-organic frameworks for co-delivery of survivin siRNA and oridonin[J]. J Colloid Interface Sci, 2023, 646: 370-380. Zhao L, Liu Z Y, Deng X Y, et al. Polyphyllin VII induces mitochondrial apoptosis by regulating the PP2A/Akt/ DRP1 signaling axis in human ovarian cancer[J]. Oncol Rep, 2021, 45(2): 513-522. Ahmad B, Rehman S U, Azizullah A, et al. Molecular mechanisms of anticancer activities of polyphyllin VII[J]. Chem Biol Drug Des, 2021, 97(4): 914-929. Xiang Y C, Peng P, Liu X W, et al. Paris saponin VII, a Hippo pathway activator, induces autophagy and exhibits therapeutic potential against human breast cancer cells[J]. Acta Pharmacol Sin, 2022, 43(6): 1568-1580. Zhang Y B, Wei S J, Zhang Q X, et al. Paris saponin VII inhibits triple-negative breast cancer by targeting the MEK/ERK/STMN1 signaling axis[J]. Phytomedicine, 2024, 130: 155746.
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