中草药

海南产对叶百部中1个新的降倍半萜

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中草药 | 化学成分 2026,57(6): 2022-2028

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中草药 |化学成分 2026 , 57 (6) : 2022 -2028

海南产对叶百部中1个新的降倍半萜

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朱雄波: 朱雄波,男,硕士研究生,研究方向为天然产物化学。E-mail:zoobox@qq.com

A new norsesquiterpenoid from Stemona tuberosa in Hainan

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doi: 10.7501/j.issn.0253-2670.2026.06.003

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目的研究海南产对叶百部Stemona tuberosa根部的化学成分及其抗炎活性。方法利用硅胶柱色谱、凝胶柱色谱、半制备高效液相色谱等色谱技术进行分离纯化，再通过核磁共振、质谱等现代波谱学方法进行鉴定，最后通过脂多糖诱导的小鼠单核巨噬细胞RAW264.7抗炎模型测试化合物的抗炎活性。结果从海南产对叶百部根中分离鉴定得到7个化合物，分别为对叶百部萜酮（1）、羟基百部叶碱（2）、4-羟基肉桂酸甲酯（3）、(-)-百部酰胺（4）、(2S,4R,9'aS)-4-甲基八氢螺[氧杂环戊烷-2,9'-吡咯并[1,2-a]氮杂䓬]-3',5-二酮（5）、2,7-二羟基-4,8-二甲氧基-1,3-二甲基-9,10-二氢菲（6）、3-[2-(3-羟基-2-甲氧基苯基)乙基]-5-甲氧基-2-甲基苯酚（7）。化合物2和3具有抑制脂多糖诱导的RAW264.7细胞NO生成的活性，半数抑制浓度（median inhibition concentration，IC₅₀）值分别为（42.99±4.96）μmol/L和（70.22±0.81）μmol/L。结论化合物1为新化合物，命名为对叶百部萜酮；化合物2为首次从海南产对叶百部中分离得到；化合物2和3在测试浓度范围内无明显细胞毒性且具有一定的抗炎活性。

关键词

对叶百部 / 生物碱 / 降倍半萜 / 抗炎活性 / 对叶百部萜酮 / 羟基百部叶碱 / 4-羟基肉桂酸甲酯

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Objective To investigate the chemical components and anti-inflammatory activities of the roots of Stemona tuberosa from Hainan province. Methods The chemical constituents of S. tuberosa were isolated and purified using chromatography techniques such as silica gel, gel column chromatography, and semi-preparative high-performance liquid chromatography (HPLC). Subsequently, the structures of the purified compounds were identified by modern spectroscopic methods such as nuclear magnetic resonance (NMR) and mass spectrometry (MS). Finally, the anti-inflammatory activities of the compounds were evaluated using the lipopolysaccharide (LPS)-induced murine monocyte-macrophage RAW264.7 cell model. Results A total of seven compounds were isolated from S. tuberosa, and identified as (4R)-3,5,5-trimethyl-4-(3-methylbut-2-enoyl)cyclohex-2-en-1-one (1 ), oxystemofoline (2 ), methyl 4-hydroxycinnamate (3 ), (-)-stemoamide (4 ), tuberostemospiroline (5 ), stemanthrene C (6 ), stilbostemin F (7 ). Compounds 2 and 3 exhibited inhibitory activity against NO production in LPS-induced RAW264.7 cells, with median inhibition concentration (IC₅₀) values of (42.99 ± 4.96) and (70.22 ± 0.81) μmol/L, respectively. Conclusion Compound 1 was identified as a new compound, named tuberostemoterpenone. Compound 2 was isolated from S. tuberosa for the first time. Compounds 2 and 3 exhibited no significant cytotoxicity within the tested concentration range and demonstrated certain anti-inflammatory activity.

Key words

Stemona tuberosa Lour. / alkaloids / norsesquiterpenoids / anti-inflammatory activity / tuberostemoterpenone / oxystemofoline / methyl 4-hydroxycinnamate

引用本文

朱雄波, 许瑞华, 万博文, 吴妃, 魏艳梅, 蔡彩虹, 王昊, 戴好富, 梅文莉, 黄圣卓, 王若仲. 海南产对叶百部中1个新的降倍半萜. 中草药, 2026 , 57 (6) : 2022 -2028 . DOI: 10.7501/j.issn.0253-2670.2026.06.003

ZHU Xiongbo, XU Ruihua, WAN Bowen, WU Fei, WEI Yanmei, CAI Caihong, WANG Hao, DAI Haofu, MEI Wenli, HUANG Shengzhuo, WANG Ruozhong. A new norsesquiterpenoid from Stemona tuberosa in Hainan[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (6) : 2022 -2028 . DOI: 10.7501/j.issn.0253-2670.2026.06.003

基金

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海南省重点研发项目 (ZDYF2024SHFZ063); 国家自然科学基金面上项目 (82373763); 国家热带农业科学中心科技创新团队 (CATASCXTD202521)

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2026年第57卷第6期

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doi: 10.7501/j.issn.0253-2670.2026.06.003

接收时间：2025-12-21
首发时间：2026-09-09

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收稿日期：2025-12-21

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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