中草药

人参不定根皂苷生物合成分子调控与培养策略研究进展

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中草药 | 综述 2026,57(5): 1971-1981

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中草药 |综述 2026 , 57 (5) : 1971 -1981

人参不定根皂苷生物合成分子调控与培养策略研究进展

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张慧¹, 吕新芳¹

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通讯作者:

吕新芳

作者简介:

张慧: 张慧,女,硕士研究生,研究方向为分子生物学。E-mail:2998231375@qq.com

Advances in molecular regulation and cultivation strategies of ginsenoside biosynthesis in Panax ginseng adventitious roots

ZHANG Hui, LYU Xinfang

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doi: 10.7501/j.issn.0253-2670.2026.05.032

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摘要

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人参Panax ginseng是一种兼具药用和食用价值的珍贵中药材，其主要活性成分为人参皂苷。受限于野生资源匮乏、市场需求旺盛及传统栽培模式的局限，构建高效可控的体外培养体系已成为研究热点。结合近年来的多组学研究成果，综述了人参皂苷的生物合成通路及其关键酶基因的表达调控机制，系统探讨了人参不定根培养的优势及其在次生代谢产物生产中的应用潜力，重点分析了培养基优化、诱导策略和环境因子对人参皂苷生产的影响。未来研究应进一步揭示皂苷生物合成中的后修饰步骤，整合多组学数据构建动态代谢模型，并优化绿色诱导策略，以加速人参不定根培养技术向规模化、工业化生产的转化。

关键词

人参不定根 / 人参皂苷 / 人参皂苷合成通路 / 多组学分析 / 培养条件

Abstract

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Panax ginseng is a valuable traditional Chinese medicinal herb with both therapeutic and nutritional properties, and ginsenosides are its principal active components. Due to the limited availability of wild resources, increasing market demand, and constraints of conventional cultivation methods, the development of an efficient and controllable in vitro culture system has become a major research focus. Based on recent multi-omics studies, this review summarizes the biosynthetic pathways of ginsenosides and the regulatory mechanisms of their key enzyme genes. It systematically discusses the advantages of adventitious root culture and its potential applications in the production of secondary metabolites, with particular emphasis on the effects of medium optimization, elicitor strategies, and environmental factors on ginsenoside production. Future studies should aim to uncover the post-modification steps in ginsenoside biosynthesis, integrate multi-omics data to construct dynamic metabolic models, and refine eco-friendly elicitation strategies to facilitate the industrial-scale application of adventitious root culture technology in P. ginseng production.

Key words

Panax ginseng C. A. Meyer / adventitious root / ginsenoside / ginsenoside synthesis pathway / multi-omics analysis / culture conditions

引用本文

张慧, 吕新芳. 人参不定根皂苷生物合成分子调控与培养策略研究进展. 中草药, 2026 , 57 (5) : 1971 -1981 . DOI: 10.7501/j.issn.0253-2670.2026.05.032

ZHANG Hui, LYU Xinfang. Advances in molecular regulation and cultivation strategies of ginsenoside biosynthesis in Panax ginseng adventitious roots[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (5) : 1971 -1981 . DOI: 10.7501/j.issn.0253-2670.2026.05.032

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魏来, 罗志勇. 人参皂苷生物合成及关键酶的研究进展[J]. 湖南中医杂志, 2017, 33(10): 212-215.
王超, 孙浩栋, 魏凤凤, 等. 人参皂苷Rb₁改善心力衰竭的研究进展[J]. 现代药物与临床, 2023, 38(3): 731-737.
Wu J, Zhong J J. Production of ginseng and its bioactive components in plant cell culture: Current technological and applied aspects[J]. J Biotechnol, 1999, 68(2/3): 89-99.
Xu F J, Valappil A K, Mathiyalagan R, et al. In vitro cultivation and ginsenosides accumulation in Panax ginseng: A review[J]. Plants, 2023, 12(17): 3165.
尹双双, 高文远, 王娟, 等. 药用植物不定根培养的影响因素[J]. 中国中药杂志, 2012, 37(24): 3691-3694.
Adil M, Jeong B R. In vitro cultivation of Panax ginseng C.A. Meyer[J]. Ind Crops Prod, 2018, 122: 239-251.
Szymańska G, Kochan E, Szymczyk P. Field cultivation and in vitro cultures, root-forming callus cultures and adventitious root cultures, of Panax quinquefolium as a source of ginsenosides[J]. Z Naturforsch C J Biosci, 2013, 68(11/12): 482-488.
Murthy H N, Dandin V S, Paek K Y. Tools for biotechnological production of useful phytochemicals from adventitious root cultures[J]. Phytochem Rev, 2016, 15(1): 129-145.
左北梅, 高文远, 董艳艳, 等. 药用植物人参的组织培养研究进展[J]. 中国现代中药, 2012, 14(1): 34-37.
Huang T, Gao W Y, Wang J, et al. Selection and optimization of a high-producing tissue culture of Panax ginseng C. A. Meyer[J]. Acta Physiol Plant, 2010, 32(4): 765-772.
Kim Y J, Zhang D B, Yang D C. Biosynthesis and biotechnological production of ginsenosides[J]. Biotechnol Adv, 2015, 33(6 Pt 1): 717-735.
Nag S A, Qin J J, Wang W, et al. Ginsenosides as anticancer agents: in vitro and in vivo activities, structure-activity relationships, and molecular mechanisms of action[J]. Front Pharmacol, 2012, 3: 25.
Yuan C S, Wang C Z, Wicks S M, et al. Chemical and pharmacological studies of saponins with a focus on American ginseng[J]. J Ginseng Res, 2010, 34(3): 160-167.
房晓雪. 栽培人参的驯化模式及其与野生人参皂苷含量差异的转录调控机制[D]. 长春: 东北师范大学, 2024.
Liang Y, Zhao S. Progress in understanding of ginsenoside biosynthesis[J]. Plant Biol, 2008, 10(4): 415-421.
Luthra R, Roy A, Pandit S, et al. Biotechnological methods for the production of ginsenosides[J]. S Afr N J Bot, 2021, 141: 25-36.
Bach T J, Boronat A, Caelles C, et al. Aspects related to mevalonate biosynthesis in plants[J]. Lipids, 1991, 26(8): 637-648.
Hou M Q, Wang R F, Zhao S J, et al. Ginsenosides in Panax genus and their biosynthesis[J]. Acta Pharm Sin B, 2021, 11(7): 1813-1834.
明乾良, 韩婷, 黄芳, 等. 人参皂苷生物合成途径及其相关酶的研究进展[J]. 中草药, 2010, 41(11): 1913-1917.
季宏与, 丁常宏, 于丹, 等. 转录组学技术在人参皂苷生物合成途径研究中的应用进展[J]. 食品与发酵工业, 2022, 48(16): 318-325.
Wang Z, Gerstein M, Snyder M. RNA-Seq: A revolutionary tool for transcriptomics[J]. Nat Rev Genet, 2009, 10(1): 57-63.
Jayakodi M, Lee S C, Park H S, et al. Transcriptome profiling and comparative analysis of Panax ginseng adventitious roots[J]. J Ginseng Res, 2014, 38(4): 278-288.
Bin K K, Jayakodi M, Lee Y S, et al. Identification of candidate UDP-glycosyltransferases involved in protopanaxadiol-type ginsenoside biosynthesis in Panax ginseng[J]. Sci Rep, 2018, 8(1): 11744.
Jayakodi M, Lee S C, Lee Y S, et al. Comprehensive analysis of Panax ginseng root transcriptomes[J]. BMC Plant Biol, 2015, 15: 138.
Koo H, Lee Y S, Nguyen V B, et al. Comparative transcriptome and metabolome analyses of four Panax species explore the dynamics of metabolite biosynthesis[J]. J Ginseng Res, 2023, 47(1): 44-53.
Lee Y S, Park H S, Lee D K, et al. Comparative analysis of the transcriptomes and primary metabolite profiles of adventitious roots of five Panax ginseng cultivars[J]. J Ginseng Res, 2017, 41(1): 60-68.
Lee Y S, Park H S, Lee D K, et al. Integrated transcriptomic and metabolomic analysis of five Panax ginseng cultivars reveals the dynamics of ginsenoside biosynthesis[J]. Front Plant Sci, 2017, 8: 1048.
Subramaniyam S, Mathiyalagan R, Natarajan S, et al. Transcript expression profiling for adventitious roots of Panax ginseng Meyer[J]. Gene, 2014, 546(1): 89-96.
Wu X H, Fan M Z, Li X F, et al. Involvement of putrescine, nitric oxide, and hydrogen peroxide in methyl jasmonate-induced ginsenoside synthesis in adventitious root cultures of Panax ginseng C. A. Meyer[J]. J Plant Growth Regul, 2021, 40(4): 1440-1449.
Lambert E, Faizal A, Geelen D. Modulation of triterpene saponin production: In vitro cultures, elicitation, and metabolic engineering[J]. Appl Biochem Biotechnol, 2011, 164(2): 220-237.
Lee M H, Jeong J H, Seo J W, et al. Enhanced triterpene and phytosterol biosynthesis in Panax ginseng overexpressing squalene synthase gene[J]. Plant Cell Physiol, 2004, 45(8): 976-984.
Oh J Y, Kim Y J, Jang M G, et al. Investigation of ginsenosides in different tissues after elicitor treatment in Panax ginseng[J]. J Ginseng Res, 2014, 38(4): 270-277.
向文珍, 张贺, 王鑫, 等. 茉莉酸甲酯诱导刺人参不定根三萜皂苷合成及其关键酶基因表达研究[J]. 中药材, 2024, 47(9): 2146-2153.
Zhang R, Huang J J, Zhu J, et al. Isolation and characterization of a novel PDR-type ABC transporter gene PgPDR3 from Panax ginseng C. A. Meyer induced by methyl jasmonate[J]. Mol Biol Rep, 2013, 40(11): 6195-6204.
Cao H Z, Nuruzzaman M, Xiu H, et al. Transcriptome analysis of methyl jasmonate-elicited Panax ginseng adventitious roots to discover putative ginsenoside biosynthesis and transport genes[J]. Int J Mol Sci, 2015, 16(2): 3035-3057.
Wang J, Li J X, Li J L, et al. Transcriptome profiling shows gene regulation patterns in ginsenoside pathway in response to methyl jasmonate in Panax quinquefolium adventitious root[J]. Sci Rep, 2016, 6: 37263.
Li J X, Li H F, Liu D H, et al. Analysis of ginsenoside content, functional genes involved in ginsenosides biosynthesis, and activities of antioxidant enzymes in Panax quinquefolium L. adventitious roots by fungal elicitors[J]. Res Chem Intermed, 2017, 43(4): 2415-2432.
梁佳, 苗佳琪, 郝甜甜, 等. 人参不定根诱导、增殖及皂苷积累的研究[J]. 中药材, 2023, 46(10): 2382-2387.
Kim Y J, Joo S C, Shi J X, et al. Metabolic dynamics and physiological adaptation of Panax ginseng during development[J]. Plant Cell Rep, 2018, 37(3): 393-410.
Chen H, Li X Z, Zheng Y J, et al. Effects of different culture times genes expression on ginsenoside biosynthesis of the ginseng adventitious roots in Panax ginseng[J]. Horticulturae, 2023, 9(7): 762.
Cui X H, Murthy H N, Zhang J D, et al. Effect of nutritional factors on the accretion of secondary metabolites in Malaysian ginseng adventitious root cultures[J]. Plant Biotechnol Rep, 2020, 14(3): 381-386.
Sivakumar G, Yu K W, Paek K Y. Production of biomass and ginsenosides from adventitious roots of Panax ginseng in bioreactor cultures[J]. Eng Life Sci, 2005, 5(4): 333-342.
Yu K W, Gao W Y, Hahn E J, et al. Effects of macro elements and nitrogen source on adventitious root growth and ginsenoside production in ginseng (Panax ginseng C. A. Meyer)[J]. J Plant Biol, 2001, 44(4): 179-184.
Paek K Y, Murthy H N, Hahn E J, et al. Biotechnology in China I[M]. Berlin: Springer, 2009: 151-176.
邱茹玥, 窦维泽, 张思瑜, 等. 基于转录组分析铵态氮与硝态氮对人参不定根皂苷积累的影响[J/OL]. 作物杂志, (2024-08-22)[2025-09-27]. https://link.cnki.net/ urlid/11.1808.s.20240820.1917.002.
Liu H, Wang J, Gao W Y, et al. Optimization and quality assessment of adventitious roots culture in Panax quinquefolium L.[J]. Acta Physiol Plant, 2014, 36(3): 713-719.
Kevers C, Jacques P, Thonart P, et al. In vitro root cultures of Panax ginseng and P. quinquefolium[J]. Plant Growth Regul, 1999, 27(3): 173-179.
Dewir Y H, Chakrabarty D, Wu C H, et al. Influences of polyunsaturated fatty acids (PUFAs) on growth and secondary metabolite accumulation in Panax ginseng C. A. Meyer adventitious roots cultured in air-lift bioreactors[J]. S Afr N J Bot, 2010, 76(2): 354-358.
Jeong C S, Murthy H N, Hahn E J, et al. Improved production of ginsenosides in suspension cultures of ginseng by medium replenishment strategy[J]. J Biosci Bioeng, 2008, 105(3): 288-291.
Li X Z, Zheng Y J, Liu M M, et al. Weighted gene co-expression network analysis and identification of ginsenoside biosynthesis candidate genes for ginseng adventitious roots under MeJA treatment[J]. Genes Genomics, 2024, 46(12): 1473-1485.
Ali M B, Hahn E J, Paek K Y. Copper-induced changes in the growth, oxidative metabolism, and saponin production in suspension culture roots of Panax ginseng in bioreactors[J]. Plant Cell Rep, 2006, 25(10): 1122-1132.
Ali M B, Yu K W, Hahn E J, et al. Methyl jasmonate and salicylic acid elicitation induces ginsenosides accumulation, enzymatic and non-enzymatic antioxidant in suspension culture Panax ginseng roots in bioreactors[J]. Plant Cell Rep, 2006, 25(6): 613-620.
Kim Y S, Hahn E J, Murthy H N, et al. Adventitious root growth and ginsenoside accumulation in Panax ginseng cultures as affected by methyl jasmonate[J]. Biotechnol Lett, 2004, 26(21): 1619-1622.
Yu K W, Gao W Y, Hahn E J, et al. Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C. A. Meyer[J]. Biochem Eng J, 2002, 11(2/3): 211-215.
Kim Y S, Yeung E C, Hahn E J, et al. Combined effects of phytohormone, indole-3-butyric acid, and methyl jasmonate on root growth and ginsenoside production in adventitious root cultures of Panax ginseng C. A. Meyer[J]. Biotechnol Lett, 2007, 29(11): 1789-1792.
Bae K H, Choi Y E, Shin C G, et al. Enhanced ginsenoside productivity by combination of ethephon and methyl jasmoante in ginseng (Panax ginseng C. A. Meyer) adventitious root cultures[J]. Biotechnol Lett, 2006, 28(15): 1163-1166.
Yu K W, Murthy H N, Jeong C S, et al. Organic germanium stimulates the growth of ginseng adventitious roots and ginsenoside production[J]. Process Biochem, 2005, 40(9): 2959-2961.
Tewari R K, Lee S Y, Hahn E J, et al. Temporal changes in the growth, saponin content and antioxidant defense in the adventitious roots of Panax ginseng subjected to nitric oxide elicitation[J]. Plant Biotechnol Rep, 2007, 1(4): 227-235.
Tewari R K, Paek K Y. Salicylic acid-induced nitric oxide and ROS generation stimulate ginsenoside accumulation in Panax ginseng roots[J]. J Plant Growth Regul, 2011, 30(4): 396-404.
于靖辉. 人参内生菌广泛靶向代谢组学分析及其对人参不定根的影响[D]. 长春: 吉林农业大学, 2023.
Wang S H, Liang W X, Lu J, et al. Penicillium sp. YJM-2013 induces ginsenosides biosynthesis in Panax ginseng adventitious roots by inducing plant resistance responses[J]. Chin Herb Med, 2020, 12(3): 257-264
Song X L, Wu H, Yin Z H, et al. Endophytic bacteria isolated from Panax ginseng improves ginsenoside accumulation in adventitious ginseng root culture[J]. Molecules, 2017, 22(6): 837.
Xu X D, Liang W X, Yao L, et al. Production of ginsenoside by Chaetomium sp. and its effect on enhancing the contents of ginsenosides in Panax ginseng adventitious roots[J]. Biochem Eng J, 2021, 174: 108100.
Li J X, Liu S J, Wang J, et al. Fungal elicitors enhance ginsenosides biosynthesis, expression of functional genes as well as signal molecules accumulation in adventitious roots of Panax ginseng C. A. Mey[J]. J Biotechnol, 2016, 239: 106-114.
王诗惠. 温度和固定化黑曲霉对人参不定根中人参皂苷含量的调控研究[D]. 天津: 天津科技大学, 2019.
Hao Y J, An X L, Sun H D, et al. Ginsenoside synthesis of adventitious roots in Panax ginseng is promoted by fungal suspension homogenate of Alternaria panax and regulated by several signaling molecules[J]. Ind Crops Prod, 2020, 150: 112414.
An X L, Yu Y, Fan M Z, et al. A fungal Mycelium elicitor efficiently improved ginsenoside synthesis during adventitious root culture of Panax ginseng[J]. J Plant Biochem Biotechnol, 2022, 31(3): 657-664.
Le K C, Im W T, Paek K Y, et al. Biotic elicitation of ginsenoside metabolism of mutant adventitious root culture in Panax ginseng[J]. Appl Microbiol Biotechnol, 2018, 102(4): 1687-1697.
Kim D S, Kim S Y, Jeong I Y, et al. Improvement of ginsenoside production by Panax ginseng adventitious roots induced by γ-irradiation[J]. Biol Plant, 2009, 53(3): 408-414.
Kim D S, Song M, Kim S H, et al. The improvement of ginsenoside accumulation in Panax ginseng as a result of γ-irradiation[J]. J Ginseng Res, 2013, 37(3): 332-340.
Zhang J Y, Sun H J, Song I J, et al. Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation ⁶⁰Co of adventitious roots[J]. J Ginseng Res, 2014, 38(3): 220-225.
Le K C, Ho T T, Paek K Y, et al. Low dose gamma radiation increases the biomass and ginsenoside content of callus and adventitious root cultures of wild ginseng (Panax ginseng Mayer)[J]. Ind Crops Prod, 2019, 130: 16-24.
Wu J Y, Wong K, Ho K P, et al. Enhancement of saponin production in Panax ginseng cell culture by osmotic stress and nutrient feeding[J]. Enzyme Microb Technol, 2005, 36(1): 133-138.
Wang S H, Liang W X, Yao L, et al. Effect of temperature on morphology, ginsenosides biosynthesis, functional genes, and transcriptional factors expression in Panax ginseng adventitious roots[J]. J Food Biochem, 2019, 43(4): e12794.
袁元. 低温处理对人参不定根中人参皂苷Rg₁和Re积累的影响[D]. 延吉: 延边大学, 2020.
Yu K W, Murthy H N, Hahn E J, et al. Ginsenoside production by hairy root cultures of Panax ginseng: Influence of temperature and light quality[J]. Biochem Eng J, 2005, 23(1): 53-56.
Kim Y J, Lee O R, Oh J Y, et al. Functional analysis of 3-hydroxy-3-methylglutaryl coenzyme a reductase encoding genes in triterpene saponin-producing ginseng[J]. Plant Physiol, 2014, 165(1): 373-387.
翟兵中, 曲雪峰, 胡文力, 等. 人参不定根生产、生物活性成分及应用现状[J]. 食品安全质量检测学报, 2021, 12(15): 6097-6104.
杨金玲, 高丽丽, 朱平. 人参皂苷生物合成研究进展[J]. 药学学报, 2013, 48(2): 170-178.

2026年第57卷第5期

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doi: 10.7501/j.issn.0253-2670.2026.05.032

接收时间：2025-10-31
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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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