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基于在体单向肠灌流和Caco-2细胞模型的金振口服液肠吸收特性研究

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中草药 | 药理与临床 2026,57(4): 1391-1401

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中草药 |药理与临床 2026 , 57 (4) : 1391 -1401

基于在体单向肠灌流和Caco-2细胞模型的金振口服液肠吸收特性研究

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常榕蓉¹, 尹兴斌¹, 冯洋¹, 孙宇菲¹, 荆霄鸿¹, 尹东阁¹, 董晓旭¹, 曲昌海¹, 王振中^2,3, 王团结^2,3, 雷海民¹, 倪健¹, 肖伟^2,3

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通讯作者:

倪健

作者简介:

常榕蓉: 常榕蓉（2001—），女，硕士研究生，研究方向为中药新剂型与新技术。E-mail:19863675636@163.com

Study on intestinal absorption characteristics of Jinzhen Oral Liquid based on in situ single-pass intestinal perfusion and Caco-2 cell model

CHANG Rongrong, YIN Xingbin, FENG Yang, SUN Yufei, JING Xiaohong, YIN Dongge, DONG Xiaoxv, QV Changhai, WANG Zhenzhong, WANG Tuanjie, LEI Haimin, NI Jian, XIAO Wei

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doi: 10.7501/j.issn.0253-2670.2026.04.017

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目的采用大鼠在体单向肠灌流与Caco-2细胞模型系统探究金振口服液中黄芩苷、甘草酸、芦荟大黄素、汉黄芩素、大黄素、大黄酚6种成分的肠吸收特性。方法体外实验借助Caco-2单层细胞模型，通过表观渗透系数（apparent permeability coefficient，Papp）初步评价6种成分的吸收能力；体内实验借助大鼠在体肠灌流，通过吸收速率常数（Ka）和有效渗透系数（effective permeability coefficient，Peff）进一步验证6种成分在大鼠肠段（十二指肠、空肠、回肠以及结肠）的吸收情况；对比分析体内外吸收规律并解析金振口服液中6种成分吸收规律。结果体内外实验结果呈现较好的一致性。在低、中、高3种浓度灌流条件下，6种成分在大鼠的各个肠段均具有可吸收性。其中Peff值在十二指肠、空肠、结肠中均大于1.2×10−³ cm/min，表明6种成分在该3肠段均吸收良好；在回肠段，黄芩苷、甘草酸、汉黄芩素Peff值为1.8×10−⁴～1.2×10−³ cm/min，表明该3种成分在回肠段吸收情况适中；芦荟大黄素、大黄素、大黄酚Peff值大于1.2×10−³ cm/min，表明该3种成分在回肠肠段吸收良好。另外，该6种成分的吸收机制存在差异，且多数成分存在主动转运、促进扩散等需载体参与的吸收特征（部分呈浓度相关性或饱和性），同时也有成分以被动扩散为主或主动与被动转运共同参与。结论体内外肠吸收实验揭示了金振口服液中6种主要成分的肠段特异性吸收规律，其整体吸收情况良好，符合口服制剂的要求。

关键词

金振口服液 / 肠吸收特性 / 在体单向肠灌流 / Caco-2细胞模型 / 黄芩苷 / 甘草酸 / 芦荟大黄素 / 汉黄芩素 / 大黄素 / 大黄酚

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Objective To systematically investigate the intestinal absorption characteristics of baicalin, glycyrrhizic acid, aloe-emodin, wogonin, emodin and chrysophanol in Jinzhen Oral Liquid (金振口服液) using the rat in situ single-pass intestinal perfusion model and Caco-2 cell model. Methods In vitro experiments were conducted using Caco-2 monolayer cell model to preliminarily evaluate the absorption capacity of the six components through apparent permeability coefficient (P_app). In situ experiments were conducted using rats in situ intestinal perfusion to further verify the absorption of the six components in rat intestinal segments (duodenum, jejunum, ileum, and colon) through the absorption rate constant (K_a) and effective permeability coefficient (P_eff). The in vitro and in situ absorption rules were compared and analyzed to clarify their absorption mechanisms. Results The in vitro and in situ experimental results showed good consistency. Under low-, medium-, and high-concentration perfusion conditions, all six components were absorbable in various intestinal segments of rats. Among them, the P_eff values in the duodenum, jejunum, and colon were all greater than 1.2 × 10⁻³ cm/min, indicating that the six components were well absorbed in these three intestinal segments. In the ileum segment, the P_eff values of baicalin, glycyrrhizic acid and wogonin were 1.8 × 10⁻⁴—1.2 × 10⁻³ cm/min, indicating moderate absorption of these three components in the ileum. The P_eff values of aloe-emodin, emodin and chrysophanol were greater than 1.2 × 10⁻³ cm/min, indicating good absorption of these three components in the ileum. In addition, there were differences in the absorption mechanisms of these six components, and most of them exhibited absorption characteristics that required carrier involvement, such as active transport and diffusion promotion (some of which were concentration dependent or saturated). At the same time, there were also components that mainly participate in passive diffusion or both active and passive transport. Conclusion In situ and in vitro intestinal absorption experiments reveal the segment-specific absorption patterns of six major components in Jinzhen Oral Liquid, with good overall absorption that meets the requirements for oral preparations.

Key words

Jinzhen Oral Liquid / intestinal absorption characteristics / in situ single-pass intestinal perfusion / Caco-2 cell model / baicalin / glycyrrhizic acid / aloe-emodin / wogonin / emodin / chrysophanol

引用本文

常榕蓉, 尹兴斌, 冯洋, 孙宇菲, 荆霄鸿, 尹东阁, 董晓旭, 曲昌海, 王振中, 王团结, 雷海民, 倪健, 肖伟. 基于在体单向肠灌流和Caco-2细胞模型的金振口服液肠吸收特性研究. 中草药, 2026 , 57 (4) : 1391 -1401 . DOI: 10.7501/j.issn.0253-2670.2026.04.017

CHANG Rongrong, YIN Xingbin, FENG Yang, SUN Yufei, JING Xiaohong, YIN Dongge, DONG Xiaoxv, QV Changhai, WANG Zhenzhong, WANG Tuanjie, LEI Haimin, NI Jian, XIAO Wei. Study on intestinal absorption characteristics of Jinzhen Oral Liquid based on in situ single-pass intestinal perfusion and Caco-2 cell model[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (4) : 1391 -1401 . DOI: 10.7501/j.issn.0253-2670.2026.04.017

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国家工信部项目 (TC2308068); 国家中医药管理局高水平建设学科 (zyyzdxk-2023272)

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2026年第57卷第4期

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doi: 10.7501/j.issn.0253-2670.2026.04.017

接收时间：2025-09-01
首发时间：2026-09-09

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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