中草药

余甘子果实中具有抗氧化和降糖活性成分研究

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中草药 | 化学成分 2026,57(4): 1221-1227

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中草药 |化学成分 2026 , 57 (4) : 1221 -1227

余甘子果实中具有抗氧化和降糖活性成分研究

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罗甸^1,2, 杨敏¹, 王晨阳¹, 李维薇³, 朱宏涛¹, 杨力颖⁴, 张颖君¹

作者信息

通讯作者:

张颖君

作者简介:

罗甸: 罗甸，博士研究生，研究方向为天然药物化学。E-mail:luodian@mail.kib.ac.cn

Chemical constituents from fruits of Phyllanthus emblica and their antioxidant and hypoglycemic activities

LUO Dian, YANG Min, WANG Chenyang, LI Weiwei, ZHU Hongtao, YANG Liying, ZHANG Yingjun

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doi: 10.7501/j.issn.0253-2670.2026.04.002

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目的研究余甘子Phyllanthus emblica果实的化学成分，并评价其抗氧化与降糖活性。方法采用硅胶和反相硅胶RP-18柱色谱以及半制备高效液相色谱等技术进行分离纯化，根据化合物理化性质，应用UV、NMR、HR-ESI-MS及X射线单晶衍射等手段并结合文献数据鉴定化合物结构。通过DPPH自由基清除实验和α-葡萄糖苷酶抑制实验评价抗氧化和降糖活性。结果从余甘子果实甲醇提取物的醋酸乙酯萃取部位中分离得到9个化合物，分别鉴定为1-(2-aminobenzofuran-3-yl)-2-(2-methoxyphenyl)ethan-1-one（1）、methyl dioxindole-3-acetate（2）、橙皮酰胺（3）、叶下珠酯G3（4）、mucic acid dimethyl ester 2-O-gallate（5）、诃子酸三甲酯（6）、3,4,8,9,10-pentahydroxy- dibenzo[b,d]pyran-6-one（7）、鞣花酸（8）和甲基 1-O-苯甲酰基-3-α-葡萄糖醛酸基甘油酯（9）。活性筛选显示，化合物5～8具有显著的DPPH自由基清除能力（清除率为86.59%～90.75%）；化合物1、7和8对α-葡萄糖苷酶表现出抑制活性，半数抑制浓度（median inhibition concentration，IC₅₀）为1.44～33.06 μmol/L，其中化合物7的抑制活性最强，优于阳性对照槲皮素（IC₅₀＝4.96 μmol/L）。结论化合物1为新的生物碱类化合物，命名为余甘子碱A；化合物2、3和9为首次从该植物中分离得到。首次从余甘子中鉴定出具有降糖活性的生物碱类成分，为其传统“降糖、抗氧化”功效提供了科学依据。

关键词

余甘子 / 生物碱 / 余甘子碱A / 抗氧化 / α-葡萄糖苷酶抑制 / 橙皮酰胺 / 甲基 1-O-苯甲酰基-3-α-葡萄糖醛酸基甘油酯

Abstract

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Objective To investigate the chemical constituents of the fruits of Phyllanthus emblica and evaluate their antioxidant and hypoglycemic activities. Methods Compounds were isolated and purified using silica gel and reversed-phase silica gel RP-18 column chromatography, followed with semi-preparative high-performance liquid chromatography (HPLC). The chemical structures were elucidated based on physicochemical properties, together with UV, NMR, HR-ESI-MS, and X-ray single-crystal diffraction methods, and comparison with literature data. Antioxidant and hypoglycemic activities were assessed by DPPH free radical scavenging and α-glucosidase inhibitory assays. Results Nine compounds were isolated from the ethyl acetate fraction of the methanol extract of P. emblica fruits and identified as 1-(2-aminobenzofuran-3-yl)-2-(2-methoxyphenyl)ethan-1-one (1), methyl dioxindole-3-acetate (2), aurantiamide (3), phyllaemblicin G3 (4), mucic acid dimethyl ester 2-O-gallate (5), chebulic acid trimethyl ester (6), 3,4,8,9,10-pentahydroxydibenzo[b,d]pyran-6-one (7), ellagic acid (8), and methyl 1-O-benzoyl-3-α- glucuronosyl glycerol (9), respectively. The bioassay showed that compounds 5-8 exhibited significant DPPH radical scavenging capacity (scavenging rates = 86.59% − 90.75%), and 1, 7, and 8 displayed inhibitory activity against α-glucosidase with IC₅₀ values ranging from 1.44 to 33.06 μmol/L. Among them, compound 7 showed the strongest inhibition, which was superior to the positive control quercetin (IC₅₀ = 4.96 μmol/L). Conclusion Compound 1 is a new alkaloid, named emblicine A, while 2, 3, and 9 are reported from the titled plant for the first time. It is the first study to identify alkaloid with hypoglycemic activity from P. emblica, providing a scientific basis for its traditional use in glycemic control and antioxidant defense.

Key words

Phyllanthus emblica L. / alkaloid / emblicine A / antioxidant / α-glucosidase inhibitory activity / aurantiamide / methyl 1-O-benzoyl-3-α- glucuronosyl glycerol

引用本文

罗甸, 杨敏, 王晨阳, 李维薇, 朱宏涛, 杨力颖, 张颖君. 余甘子果实中具有抗氧化和降糖活性成分研究. 中草药, 2026 , 57 (4) : 1221 -1227 . DOI: 10.7501/j.issn.0253-2670.2026.04.002

LUO Dian, YANG Min, WANG Chenyang, LI Weiwei, ZHU Hongtao, YANG Liying, ZHANG Yingjun. Chemical constituents from fruits of Phyllanthus emblica and their antioxidant and hypoglycemic activities[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (4) : 1221 -1227 . DOI: 10.7501/j.issn.0253-2670.2026.04.002

基金

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国家自然科学基金资助项目 (32270426); 国家自然科学基金资助项目 (82074124); 云南省重点研发计划 (202403AP140026)

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2026年第57卷第4期

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doi: 10.7501/j.issn.0253-2670.2026.04.002

接收时间：2025-12-03
首发时间：2026-09-09

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收稿日期：2025-12-03

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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