Article(id=1304406823159747042, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304406818550206926, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.01.006, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1762617600000, receivedDateStr=2025-11-09, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788924421348, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788924421348, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788924421348, creator=13701087609, updateTime=1788924421348, updator=13701087609, issue=Issue{id=1304406818550206926, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='1', pageStart='1', pageEnd='389', issueExtLink='null', onlineDate='null', pubDate='1768147200000', pubDateStr='2026-01-12', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788924420249, creator='13701087609', updateTime=1788924674802, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304407886289986387, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304406818550206926, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304407886289986388, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304406818550206926, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=45, endPage=52, ext={EN=ArticleExt(id=1304406823612731876, articleId=1304406823159747042, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Two new phenolic glycosides from Gentiana nanobella, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective To study the chemical constituents fromdried whole plant of alpine herb Gentiana nanobella, and to conduct preliminary screening for antimalarial activity of the isolated constituents. Methods Ethyl acetate and n-butanol fractions of 75% ethanol extract of the whole plant of G. nanobella were isolated and purified by repeated column chromatography padded with silica gel, RP-18 and Sephadex LH-20, respectively. The structures of the isolated compounds were identified based on their physicochemical properties and spectral data. The in vitro inhibitory activity of the testing compound against human Plasmodium falciparum 3D7 strain was achieved with the SYBR Green I method. Results A total of 11 phenol compounds were obtained and identified as 2,3-dihydroxy benzoic acid methyl ester-3-O-β-L-rhamnosyl-(1''→2')-b-D-glucopyranoside (1), 2,3-dihydroxy benzoic acid-3-O-β-L-rhamnosyl-(1''→2')-b-D-glucopyranoside (2), 2,3-dihydroxy benzoic acid methyl ester-3-O-D-glucopyranosyl-(1''→6')-β-D-glucopyranoside (3), benzyl β-D-xylopyranosyl-(1''→6')-β-D-glucopyranoside (4), apigenin 5-O-D-glucopyranoside (5), salcolin B (6), apigenin-7-O-D-glucopyranoside (7), benzoic acid (8), p-hydroxybenzoic acid (9), 3-hydroxy-2-methoxybenzoic acid (10), and isovanillic acid (11). Compound 6 exhibited an in vitro antimalarial activity against Plasmodium falciparum 3D7 strain with IC50 18.50 μmol/L and inhibition rate of 98.48% at a concentration of 50 μmol/L. Conclusion Compounds 1 and 2 are new compounds, named gentinanoside A and gentinanoside B. Compounds 111 were isolated from G. nanobella for the first time. G. nanobella is abundant in phenolic compounds, and some compounds had certain antimalarial activity., authors=YANG Dawei, LING Haowei, WU Jiu, GOU Liang, XU Wei, JIANG Bei, authorsList=YANG Dawei, LING Haowei, WU Jiu, GOU Liang, XU Wei, JIANG Bei, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304406823495291363, articleId=1304406823159747042, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=钟花龙胆中2个新酚苷类成分, columnId=1304140187169214944, journalTitle=中草药, columnName=化学成分, runingTitle=null, highlight=null, articleAbstract=目的 研究高寒植物钟花龙胆Gentiana nanobella干燥全株醇提物中的化学成分,并进行初步抗疟活性筛选。方法 采用硅胶、RP-18、Sephadex LH-20等多种柱色谱以及高效液相、半制备液相色谱分离手段,对钟花龙胆干燥全株进行分离纯化,根据理化性质及波谱数据鉴定化合物的结构。使用SYBR Green I方法筛选供试化合物对人体恶性疟原虫3D7株的体外抑制活性。结果 从钟花龙胆干燥全株75%乙醇提取物醋酸乙酯部位、正丁醇部位中分离鉴定了11个化合物,分别鉴定为2,3-二羟基苯甲酸甲酯-3-O-β-L-吡喃鼠李糖基-(1′′→2′)-β-D-吡喃葡萄糖苷(1)、2,3-二羟基苯甲酸-3-O-β-L-吡喃鼠李糖基-(1′′→2′)-β-D-吡喃葡萄糖苷(2)、2,3-二羟基苯甲酸甲酯-3-O-D-吡喃葡萄糖基-(1′′→6′)-β-D-吡喃葡萄糖苷(3)、苄基-β-D-吡喃木糖基-(1′′→6′)-β-D-葡萄糖苷(4)、芹菜素-5-O-D-吡喃葡萄糖苷(5)、salcolin B(6)、芹菜素-7-O-D-吡喃葡萄糖苷(7)、苯甲酸(8)、对羟基苯甲酸(9)、3-羟基-2-甲氧基苯甲酸(10)、异香草酸(11)。化合物6在50 μmol/L浓度下,体外对人体恶性疟原虫3D7株抑制率为98.48%,半数抑制浓度(median inhibition concentration,IC₅₀)值为18.50 μmol/L。结论 化合物12为新酚苷类化合物,分别命名为钟花龙胆苷A和钟花龙胆苷B;化合物111均为首次从钟花龙胆中分离得到;钟花龙胆富含酚性成分,部分化合物具有一定的抗疟活性。, authors=杨达伟1, 凌浩威1, 吴玖1, 苟亮1, 徐伟1,2, 姜北1, authorsList=杨达伟, 凌浩威, 吴玖, 苟亮, 徐伟, 姜北, authorCompany=1 大理大学 云南省滇西抗病原植物资源筛选研究重点实验室, 药物研究所, 药学院, 云南 大理 671000;
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付晓琪,张悦,徐紫晨,等.龙胆化学成分、药理作用研究进展及质量标志物预测分析[J]. 中国新药杂志,2025, 34(15):1608-1614.
叶繁庆,孙雨颉,范春雨,等.龙胆化学成分、生物活性及临床应用研究进展[J]. 中草药, 2023, 54(19):6497-6510.
陈琳.制坚龙胆的化学成分研究[D]. 昆明:昆明理工大学, 2019.
蔡爱华.关龙胆化学成分及抗肿瘤活性研究[D]. 延吉:延边大学, 2014.
翟亚东.尖叶假龙胆化学成分及抗心肌缺血作用研究[D]. 哈尔滨:黑龙江中医药大学, 2015.
王彩君,王智民,王维皓,等.龙胆属植物中的化学成分及药理活性研究进展[J]. 中国中药杂志, 2009,34(23):2987-2994.
董丽萍,倪梁红,赵志礼,等.龙胆属环烯醚萜类化学成分研究进展[J]. 中草药, 2017, 48(10):2116-2128.
凌浩威,李靖,史凯旋,等.钟花龙胆化学成分研究[J]. 大理大学学报, 2023, 8(8):6-10.
孙淼杰,王艳林,韩欣桐,等.三七根茎中黄酮类成分及其抗血小板聚集活性研究[J]. 中草药, 2025, 56(5):1500-1507.
Xu M, Yang C R, Zhang Y J. Minor antifungal aromatic glycosides from the roots of Gentiana rigescens(Gentianaceae)[J]. Chin Chem Lett, 2009, 20(10):1215-1217.
杨炳友,杨春丽,刘艳,等.洋金花根化学成分研究[J]. 中国中药杂志, 2018, 43(8):1654-1661.
来国防,赵沛基,倪志伟,等.中华青荚叶的一个新果糖酯[J]. 云南植物研究, 2008, 30(1):115-120.
Wenzig E, Kunert O, Ferreira D, et al. Flavonolignans from Avena sativa[J]. J Nat Prod, 2005, 68(2):289-292.
Abbasi M A, Lodhi M A, Ahmad V U, et al. Kinetics studies on flavone glycosides:Inhibitors of α-chymotrypsin[J]. Chem Nat Compd, 2011, 47(5):693-696.
Muratova M S, Zou G A, Jenis J, et al. Chemical constituents of Alhagi sparsifolia[J]. Chem Nat Compd,2019, 55(5):932-933.
赵家琪,郝吉,马远任,等.赶山鞭化学成分的研究[J]. 中成药, 2021, 43(3):664-669.
Luo J R, Jiang H E, Zhao Y X, et al. Components of the heartwood of Populus euphratica from an ancient tomb[J]. Chem Nat Compd, 2008, 44(1):6-9.
袁谱龙,王雪萍,陈凯先,等.水茄茎化学成分的研究[J]. 中成药, 2016, 38(1):104-107.
周伟,秦艳梅,刘小莉.云南药用龙胆资源分布和评价[J]. 云南中医中药杂志, 2015, 36(10):81-83.
Baba S A, Malik S A. Evaluation of antioxidant and antibacterial activity of methanolic extracts of Gentiana kurroo Royle[J]. Saudi J Biol Sci, 2014, 21(5):493-498.)
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钟花龙胆中2个新酚苷类成分
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中草药 | 化学成分 2026,57(1): 45-52
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中草药 |化学成分 2026 , 57 (1) : 45 -52
钟花龙胆中2个新酚苷类成分
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杨达伟1, 凌浩威1, 吴玖1, 苟亮1, 徐伟1,2, 姜北1
作者信息
    1 大理大学 云南省滇西抗病原植物资源筛选研究重点实验室, 药物研究所, 药学院, 云南 大理 671000;
    2 筋骨病中医药防治南充市重点实验室, 南充市中医医院 中药制剂中心, 四川 南充 637000
通讯作者:
姜北
作者简介:
杨达伟: 杨达伟(2002-),男,硕士研究生,研究方向为天然药物化学。E-mail:1617892860@qq.com
Two new phenolic glycosides from Gentiana nanobella
  • YANG Dawei, LING Haowei, WU Jiu, GOU Liang, XU Wei, JIANG Bei
  • Affiliations
    doi: 10.7501/j.issn.0253-2670.2026.01.006
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    目的 研究高寒植物钟花龙胆Gentiana nanobella干燥全株醇提物中的化学成分,并进行初步抗疟活性筛选。方法 采用硅胶、RP-18、Sephadex LH-20等多种柱色谱以及高效液相、半制备液相色谱分离手段,对钟花龙胆干燥全株进行分离纯化,根据理化性质及波谱数据鉴定化合物的结构。使用SYBR Green I方法筛选供试化合物对人体恶性疟原虫3D7株的体外抑制活性。结果 从钟花龙胆干燥全株75%乙醇提取物醋酸乙酯部位、正丁醇部位中分离鉴定了11个化合物,分别鉴定为2,3-二羟基苯甲酸甲酯-3-O-β-L-吡喃鼠李糖基-(1′′→2′)-β-D-吡喃葡萄糖苷(1)、2,3-二羟基苯甲酸-3-O-β-L-吡喃鼠李糖基-(1′′→2′)-β-D-吡喃葡萄糖苷(2)、2,3-二羟基苯甲酸甲酯-3-O-D-吡喃葡萄糖基-(1′′→6′)-β-D-吡喃葡萄糖苷(3)、苄基-β-D-吡喃木糖基-(1′′→6′)-β-D-葡萄糖苷(4)、芹菜素-5-O-D-吡喃葡萄糖苷(5)、salcolin B(6)、芹菜素-7-O-D-吡喃葡萄糖苷(7)、苯甲酸(8)、对羟基苯甲酸(9)、3-羟基-2-甲氧基苯甲酸(10)、异香草酸(11)。化合物6在50 μmol/L浓度下,体外对人体恶性疟原虫3D7株抑制率为98.48%,半数抑制浓度(median inhibition concentration,IC₅₀)值为18.50 μmol/L。结论 化合物12为新酚苷类化合物,分别命名为钟花龙胆苷A和钟花龙胆苷B;化合物111均为首次从钟花龙胆中分离得到;钟花龙胆富含酚性成分,部分化合物具有一定的抗疟活性。
    钟花龙胆  /  新酚苷类化合物  /  抗疟活性  /  钟花龙胆苷A  /  钟花龙胆苷B  /  芹菜素-5-O-D-吡喃葡萄糖苷
    Objective To study the chemical constituents fromdried whole plant of alpine herb Gentiana nanobella, and to conduct preliminary screening for antimalarial activity of the isolated constituents. Methods Ethyl acetate and n-butanol fractions of 75% ethanol extract of the whole plant of G. nanobella were isolated and purified by repeated column chromatography padded with silica gel, RP-18 and Sephadex LH-20, respectively. The structures of the isolated compounds were identified based on their physicochemical properties and spectral data. The in vitro inhibitory activity of the testing compound against human Plasmodium falciparum 3D7 strain was achieved with the SYBR Green I method. Results A total of 11 phenol compounds were obtained and identified as 2,3-dihydroxy benzoic acid methyl ester-3-O-β-L-rhamnosyl-(1''→2')-b-D-glucopyranoside (1), 2,3-dihydroxy benzoic acid-3-O-β-L-rhamnosyl-(1''→2')-b-D-glucopyranoside (2), 2,3-dihydroxy benzoic acid methyl ester-3-O-D-glucopyranosyl-(1''→6')-β-D-glucopyranoside (3), benzyl β-D-xylopyranosyl-(1''→6')-β-D-glucopyranoside (4), apigenin 5-O-D-glucopyranoside (5), salcolin B (6), apigenin-7-O-D-glucopyranoside (7), benzoic acid (8), p-hydroxybenzoic acid (9), 3-hydroxy-2-methoxybenzoic acid (10), and isovanillic acid (11). Compound 6 exhibited an in vitro antimalarial activity against Plasmodium falciparum 3D7 strain with IC50 18.50 μmol/L and inhibition rate of 98.48% at a concentration of 50 μmol/L. Conclusion Compounds 1 and 2 are new compounds, named gentinanoside A and gentinanoside B. Compounds 111 were isolated from G. nanobella for the first time. G. nanobella is abundant in phenolic compounds, and some compounds had certain antimalarial activity.
    Gentiana nanobella C. Marquand  /  new phenolic glycosides  /  antimalarial activity  /  gentinanoside A  /  gentinanoside B  /  apigenin 5-O-D-glucopyranoside
    杨达伟, 凌浩威, 吴玖, 苟亮, 徐伟, 姜北. 钟花龙胆中2个新酚苷类成分. 中草药, 2026 , 57 (1) : 45 -52 . DOI: 10.7501/j.issn.0253-2670.2026.01.006
    YANG Dawei, LING Haowei, WU Jiu, GOU Liang, XU Wei, JIANG Bei. Two new phenolic glycosides from Gentiana nanobella[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (1) : 45 -52 . DOI: 10.7501/j.issn.0253-2670.2026.01.006

      国家自然科学基金项目 (81960637,82460689); 云南省滇西抗病原植物资源筛选研究重点实验室建设项目及研究生开放课题项目 (202305AG340015,APR2024Y17)

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    宝钢,图诺木拉,朝格巴达拉夫,等.龙胆属秦艽组植物化学成分及药理作用研究进展[J]. 中成药, 2024,46(8):2689-2697.
    付晓琪,张悦,徐紫晨,等.龙胆化学成分、药理作用研究进展及质量标志物预测分析[J]. 中国新药杂志,2025, 34(15):1608-1614.
    叶繁庆,孙雨颉,范春雨,等.龙胆化学成分、生物活性及临床应用研究进展[J]. 中草药, 2023, 54(19):6497-6510.
    陈琳.制坚龙胆的化学成分研究[D]. 昆明:昆明理工大学, 2019.
    蔡爱华.关龙胆化学成分及抗肿瘤活性研究[D]. 延吉:延边大学, 2014.
    翟亚东.尖叶假龙胆化学成分及抗心肌缺血作用研究[D]. 哈尔滨:黑龙江中医药大学, 2015.
    王彩君,王智民,王维皓,等.龙胆属植物中的化学成分及药理活性研究进展[J]. 中国中药杂志, 2009,34(23):2987-2994.
    董丽萍,倪梁红,赵志礼,等.龙胆属环烯醚萜类化学成分研究进展[J]. 中草药, 2017, 48(10):2116-2128.
    凌浩威,李靖,史凯旋,等.钟花龙胆化学成分研究[J]. 大理大学学报, 2023, 8(8):6-10.
    孙淼杰,王艳林,韩欣桐,等.三七根茎中黄酮类成分及其抗血小板聚集活性研究[J]. 中草药, 2025, 56(5):1500-1507.
    Xu M, Yang C R, Zhang Y J. Minor antifungal aromatic glycosides from the roots of Gentiana rigescens(Gentianaceae)[J]. Chin Chem Lett, 2009, 20(10):1215-1217.
    杨炳友,杨春丽,刘艳,等.洋金花根化学成分研究[J]. 中国中药杂志, 2018, 43(8):1654-1661.
    来国防,赵沛基,倪志伟,等.中华青荚叶的一个新果糖酯[J]. 云南植物研究, 2008, 30(1):115-120.
    Wenzig E, Kunert O, Ferreira D, et al. Flavonolignans from Avena sativa[J]. J Nat Prod, 2005, 68(2):289-292.
    Abbasi M A, Lodhi M A, Ahmad V U, et al. Kinetics studies on flavone glycosides:Inhibitors of α-chymotrypsin[J]. Chem Nat Compd, 2011, 47(5):693-696.
    Muratova M S, Zou G A, Jenis J, et al. Chemical constituents of Alhagi sparsifolia[J]. Chem Nat Compd,2019, 55(5):932-933.
    赵家琪,郝吉,马远任,等.赶山鞭化学成分的研究[J]. 中成药, 2021, 43(3):664-669.
    Luo J R, Jiang H E, Zhao Y X, et al. Components of the heartwood of Populus euphratica from an ancient tomb[J]. Chem Nat Compd, 2008, 44(1):6-9.
    袁谱龙,王雪萍,陈凯先,等.水茄茎化学成分的研究[J]. 中成药, 2016, 38(1):104-107.
    周伟,秦艳梅,刘小莉.云南药用龙胆资源分布和评价[J]. 云南中医中药杂志, 2015, 36(10):81-83.
    Baba S A, Malik S A. Evaluation of antioxidant and antibacterial activity of methanolic extracts of Gentiana kurroo Royle[J]. Saudi J Biol Sci, 2014, 21(5):493-498.
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    2种不同金属材料的力学参数

    Family
    属数
    Number of
    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)

    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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