Article(id=1304388236294517229, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388157621948709, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.15.023, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1778083200000, receivedDateStr=2026-05-07, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788919989894, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788919989894, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788919989894, creator=13701087609, updateTime=1788919989894, updator=13701087609, issue=Issue{id=1304388157621948709, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='15', pageStart='5789', pageEnd='6208', issueExtLink='null', onlineDate='null', pubDate='1786464000000', pubDateStr='2026-08-12', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1788919971137, creator='13701087609', updateTime=1788923514106, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304403017982300207, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388157621948709, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304403017982300208, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388157621948709, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=6066, endPage=6076, ext={EN=ArticleExt(id=1304388236625867247, articleId=1304388236294517229, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Cloning, expression and carotenoid synthesis-regulating function of LbbHLH94-like in Lycium barbarum, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective This study aims to clone the LbbHLH94-like gene from Gouqi (Lycium barbarum), analyze its spatiotemporal expression, stress and hormone response patterns, and clarify its regulatory function in carotenoid biosynthesis, so as to provide a theoretical basis for revealing the molecular mechanism of goji berry fruit quality formation. Methods Using Lycium barbarum cv. ‘Ningqi 1’ as material, we employed techniques such as PCR and qRT-PCR to clone and analyze the expression patterns of LbbHLH94-like in different tissues, fruit developmental stages, abiotic stresses and exogenous hormone treatments. The overexpression vector was constructed and transformed into goji plants, and the expression of key genes, enzyme activities and total carotenoid content in carotenoid metabolism were determined. Results The LbbHLH94-like gene was successfully cloned, with an 888 bp open reading frame encoding 296 amino acids, and the sequence was identical to the genome reference sequence. This gene showed obvious spatiotemporal specificity, with the highest expression level at fruit ripening stage. It responded to high and low temperature, salt, drought stresses as well as 6-BA, IBA and GA₃ induction, showing a general pattern of promotion at low concentration and inhibition at high concentration. Overexpression of LbbHLH94-like significantly up-regulated the expression of PDS, enhanced the enzyme activities of PSY and PDS, inhibited the expression and enzyme activity of CCD4, and remarkably increased total carotenoid content in fruits. Conclusion LbbHLH94-like is a key positive regulator of carotenoid biosynthesis in goji berry, and also participates in abiotic stress response and hormone signaling. The results provide important gene resources and theoretical support for quality improvement and precise molecular breeding of Lycium barbarum., authors=LIU Shuo, LIU Xuan, HU Shi, DANG Jiali, SHE Ping, SONG Yadie, YUE Sijun, ZHENG Rui, authorsList=LIU Shuo, LIU Xuan, HU Shi, DANG Jiali, SHE Ping, SONG Yadie, YUE Sijun, ZHENG Rui, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304388236546175470, articleId=1304388236294517229, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=枸杞LbbHLH94-like克隆、表达及在类胡萝卜素合成中的调控功能研究, columnId=1304140203967410414, journalTitle=中草药, columnName=药材与资源, runingTitle=null, highlight=null, articleAbstract=目的 解析枸杞Lycium barbarum LbbHLH94-like基因的表达特征及其在类胡萝卜素合成中的调控功能，为揭示枸杞果实品质形成的分子机制提供理论依据。方法 以宁夏枸杞‘宁杞1号’为材料，采用PCR、qRT-PCR等技术克隆并分析LbbHLH94-like在不同组织、果实发育阶段、非生物胁迫及外源激素处理下的表达模式；构建过表达载体转化枸杞，分析转基因植株类胡萝卜素代谢关键基因表达、酶活性及总类胡萝卜素含量。结果 成功克隆LbbHLH94-like基因，其开放阅读框全长888 bp，编码296个氨基酸，序列与基因组参考序列一致；该基因具有明显时空表达特异性，在果实成熟期表达量最高，并响应高温、低温、盐、干旱胁迫及6-苄氨基嘌呤（6-benzylaminopurine，6-BA）、吲哚-3-丁酸（indole-3-butyric acid，IBA）、赤霉素（gibberellic acid，GA₃）诱导，外源激素整体呈低浓度促进、高浓度抑制模式；过表达LbbHLH94-like可显著上调PDS基因表达、提高PSY与PDS酶活性，抑制类胡萝卜素裂解双加氧酶4（carotenoid cleavage dioxygenase 4，CCD4）基因表达及酶活性，显著提升果实总类胡萝卜素含量。结论 LbbHLH94-like是枸杞类胡萝卜素合成的关键正向调控因子，同时参与非生物胁迫与激素信号应答，研究结果为枸杞品质改良与精准分子育种提供重要基因资源。, authors=刘朔¹, 刘璇¹, 胡诗¹, 党佳丽¹, 佘萍¹, 宋雅蝶¹, 岳思君¹, 郑蕊¹, authorsList=刘朔, 刘璇, 胡诗, 党佳丽, 佘萍, 宋雅蝶, 岳思君, 郑蕊, authorCompany=1 宁夏大学 生命科学学院, 西部特色生物资源保护与利用教育部重点实验室, 林木资源高效生产全国重点实验室, 优势特色作物现代分子育种宁夏重点实验室, 宁夏 银川 750021, correspAuthors=郑蕊, authorNote=刘朔: 刘朔，女，硕士研究生，研究方向为特色及药用植物资源保护与开发利用。E-mail:1412954721@qq.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=5F3NdnRyGzZs4XEbmWo53w==, pdfFileSize=1236150, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金资助项目 (32460098); 宁夏回族自治区重点研发计划项目 (2025BBF02004))}, authors=[Author(id=1307431848477483745, tenantId=1146029695717560320, journalId=null, articleId=1304388236294517229, orderNo=null, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=null, authorType=null, ext={EN=AuthorExt(id=null, tenantId=null, journalId=1302319053441957962, articleId=1304388236294517229, 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Zhao J H, Xu Y H, Li H X, et al. ERF5.1 modulates carotenoid accumulation by interacting with CCD4.1 in Lycium [J]. Hortic Res, 2023, 10(12): uhad230.
卢晓悦, 刘雪霞, 池礼鑫, 等. 宁夏枸杞果实转录组分析及类胡萝卜素合成相关差异表达基因的筛选[J]. 南方农业学报, 2025, 56(3): 706-719.
Donno D, Mellano M G, Gamba G, et al. Analytical strategies for fingerprinting of antioxidants, nutritional substances, and bioactive compounds in foodstuffs based on high performance liquid chromatography–mass spectrometry: An overview [J]. Foods, 2020, 9(12): 1734.
Wei F, Wan R, Shi Z G, et al. Transcriptomics and metabolomics reveal the critical genes of carotenoid biosynthesis and color formation of goji (Lycium barbarum L.) fruit ripening [J]. Plants, 2023, 12(15): 2791.
Zhang H L, Zhang S Y, Zhang H, et al. Carotenoid metabolite and transcriptome dynamics underlying flower color in marigold (Tagetes erecta L.) [J]. Sci Rep, 2020, 10(1): 16835.
刘雪霞, 范文强, 焦慧慧, 等. 基于转录组测序的宁夏枸杞不同品种果实活性成分合成差异表达基因分析[J]. 生物工程学报, 2023, 39(7): 3015-3036.
Wei Z H, Shang M Q, Jiang Z C, et al. Natural allelic variation of basic helix–loop–helix transcription factor 25 regulates carotenoid biosynthesis in sweet potato [J]. Plant Biotechnol J, 2025, 23(7): 2627-2644.
王子雨. 辣椒CaNAC035基因上游调控因子CabHLH79的鉴定及其调控机理的研究[D]. 杨凌: 西北农林科技大学,2022.
吕宝莲, 杨宇昕, 崔立操, 等. 小麦bHLH家族转录因子的鉴定及其在盐胁迫条件下的表达分析[J]. 作物杂志, 2024(1): 65-72.
刘发旺, 翟科峰, 张丽丽, 等. 王枣子bHLH转录因子家族基因挖掘及鉴定[J]. 淮北师范大学学报: 自然科学版, 2025, 46(3): 33-40.
Jia C P, Yang H T, Yang T, et al. Comprehensive metabolomic and transcriptomic analyses reveal SlSnRK_2.6/SlCHPP-SlbHLH95-SlPDS module regulating tomato fruit carotene biosynthesis [J]. J Agric Food Chem, 2025, 73(44): 28493-28510.
刘倩, 邓淑芳, 刘玲, 等. 转录因子CcbHLH107调控蜜橘果实转色的功能分析[J]. 食品科学, 2024, 45(15): 194-204.
邓明华, 莫云容, 吕俊恒, 等. 辣椒类胡萝卜素生物合成的分子遗传学研究进展[J]. 广东农业科学, 2024, 51(2): 71-80.
Zhai Y Q, Zhang L C, Xia C, et al. The wheat transcription factor, TabHLH39, improves tolerance to multiple abiotic stressors in transgenic plants [J]. Biochem Biophys Res Commun, 2016, 473(4): 1321-1327.
钱婷, 赵凡, 张玉洁, 等. 肋果沙棘和西藏沙棘转录因子bHLH94基因对海拔适应性分化的研究[J]. 植物研究, 2022, 42(6): 976-985.
Ren Y R, Zhao Q, Zhao X Y, et al. Expression analysis of the MdCIbHLH1 gene in apple flower buds and seeds in the process of dormancy [J]. Hortic Plant J, 2016, 2(2): 61-66.
Nie J, Jiang Y, Lv L J, et al. The bHLH transcription factor CsPIF4 positively regulates high temperature-induced hypocotyl elongation in cucumber [J]. Hortic Plant J, 2024, 10(5): 1187-1197.
惠甜, 沈兵琪, 王连春, 等. 桑树bHLH转录因子家族全基因组鉴定与分析[J]. 分子植物育种, 2019, 17(17): 78-93.
Seo J S, Joo J, Kim M J, et al. OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice [J]. Plant J, 2011, 65(6): 907-921.
Teng Y T, Lv M, Zhang X X, et al. BEAR1 a bHLH transcription factor, controls salt response genes to regulate rice salt response [J]. J Plant Biol, 2022, 65(3): 217-230.
刘娇妍, 朱嘉慧, 肖浩扬, 等. 水稻Os01g0853700基因对植物激素和非生物胁迫的响应分析[J]. 江苏农业科学, 2023, 51(23): 28-34.
刘强, 杨添博, 刘剑锋, 等. 叶面喷施6-BA对盐胁迫下不同种源黑果枸杞幼苗生理指标和光合特性的影响[J]. 东北林业大学学报, 2024, 52(9): 30-35.
Zhang W W, Xia L, Peng F L, et al. Transcriptomics and metabolomics changes triggered by exogenous 6-benzylaminopurine in relieving epicotyl dormancy of Polygonatum cyrtonema Hua seeds [J]. Front Plant Sci, 2022, 13: 961899.
陈紫玉, 巴哈依丁·吾甫尔, 任桂霖, 等. 外源IBA对无花果扦插苗抗氧化特性及IAA生物合成途径的影响[J]. 西北植物学报, 2024, 44(7): 1046-1054.
陈鸿, 杜金华, 马瑞娟, 等. 外源赤霉素(GA3)对不同桃品种(系)果实品质的影响[J]. 果树学报, 2025, 42(4): 775-789.
作建芬, 林益超, 刘维维, 等. 聚乙二醇-6000模拟干旱胁迫对三种牧草种子萌发及幼苗生长的影响[J]. 生态学报, 2025, 45(13): 6414-6426.
胡进红, 宋繁, 梁旺利, 等. NaCl胁迫下宁夏枸杞光合作用相关基因差异表达分析[J]. 西北植物学报, 2023, 43(1): 106-115.
刘灿, 代晓燕, 张玉银, 等. 低钾胁迫下过表达NtCaM10对烟草生长及钾素吸收的影响[J]. 中国烟草科学, 2024, 45(1): 31-38.
Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCTmethod [J]. Methods, 2001, 25(4): 402-408.
石晶, 梁新华. 甘草根系分泌物乳酸外源施用对GuSQS1和GubAS基因表达及甘草酸含量的影响[J]. 宁夏大学学报: 自然科学版, 2022, 43(1): 73-78.
Zhang L C, Kang J, Xie Q L, et al. The basic helix-loop-helix transcription factor bHLH95 affects fruit ripening and multiple metabolisms in tomato [J]. J Exp Bot, 2020, 71(20): 6311-6327.
Gan Z, Zhang Y, Yang C, et al. A kiwifruit bHLH149 transcription factor modulates carotenoid biosynthesis by directly activating LCYB during postharvest ripening [J]. Fruit Res, 2025,5: e003.
蔡欣, 黄钰杏, 崔明月, 等. 水稻OsbHLH109基因的表达分析[J]. 广东农业科学, 2023, 50(12): 120-130.
康同洋. PpbHLH59介导乙烯和脱落酸调控桃果实成熟软化的分子机制[D]. 杨凌: 西北农林科技大学, 2025.)