Article(id=1304388203545391405, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388135723496407, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.13.015, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1767801600000, receivedDateStr=2026-01-08, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788919982085, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788919982085, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788919982085, creator=13701087609, updateTime=1788919982085, updator=13701087609, issue=Issue{id=1304388135723496407, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='13', pageStart='4949', pageEnd='5352', issueExtLink='null', onlineDate='null', pubDate='1783785600000', pubDateStr='2026-07-12', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788919965916, creator='13701087609', updateTime=1788923489765, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304402915871977875, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388135723496407, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304402915871977876, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388135723496407, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=5120, endPage=5129, ext={EN=ArticleExt(id=1304388205457994032, articleId=1304388203545391405, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Mechanism of tetrandrine regulating ferroptosis of breast cancer cells mediated by peroxiredoxin 1, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective To investigate the mechanism by which tetrandrine regulates the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/glycogen synthase kinase-3β (GSK-3β) signaling pathway to induce ferroptosis in breast cancer cells based on peroxiredoxin 1 (PRDX1). Methods Human breast cancer MCF-7 cells transfected with PRDX1 siRNA were treated with tetrandrine to examine its effects on cell proliferation, apoptosis, migration and invasion. The levels of malondialdehyde (MDA), Fe2+ , intracellular and mitochondrial reactive oxygen species (ROS), mitochondrial membrane potential, and mitochondrial membrane permeability were measured. Western blotting and qRT-PCR were used to detect the expressions of voltage-dependent anion channel 1 (VDAC1), p53, PI3K/Akt/GSK-3β signaling pathway and nuclear factor E2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway related proteins and genes. Results Tetrandrine dose-dependently inhibited MCF-7 cell proliferation, migration and invasion while inducing apoptosis (P < 0.01), increased intracellular and mitochondrial ROS, MDA and Fe2+ levels (P < 0.01), aggravated lipid peroxidation, reduced mitochondrial membrane potential (P < 0.01), enhanced mitochondrial membrane permeability (P < 0.01), upregulated HO-1, p53, GSK-3β and VDAC1 expressions (P < 0.01), downregulated Nrf2, PI3K, p-PI3K, Akt and p-Akt expressions (P < 0.01). Knockdown of PRDX1 gene significantly amplified these effects of tetrandrine (P < 0.01). Conclusion Tetrandrine induces mitochondria-associated ferroptosis in MCF-7 cells via PI3K/Akt/GSK-3β signaling pathway, with PRDX1 playing a critical regulatory role., authors=XIN Guosong, SUN Yue, LIU Yuhan, HOU Yanxiu, LI Hairu, authorsList=XIN Guosong, SUN Yue, LIU Yuhan, HOU Yanxiu, LI Hairu, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304388203734135086, articleId=1304388203545391405, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=基于过氧化物还原酶1介导的粉防己碱调控乳腺癌细胞铁死亡机制研究, columnId=1304140191707456168, journalTitle=中草药, columnName=药理与临床, runingTitle=null, highlight=null, articleAbstract=目的 基于过氧化物还原酶1（peroxiredoxin 1，PRDX1）研究粉防己碱调控磷脂酰肌醇3-激酶（phosphatidylinositol 3-kinase，PI3K）/蛋白激酶B（protein kinase B，Akt）/糖原合成激酶-3β（glycogen synthesizing kinase-3β，GSK-3β）信号通路诱导乳腺癌细胞铁死亡的作用机制。方法 人乳腺癌MCF-7细胞转染PRDX1 siRNA后，给予粉防己碱干预，考察粉防己碱对MCF-7细胞增殖、凋亡、迁移和侵袭能力的影响；检测丙二醛（malondialdehyde，MDA）、Fe²⁺、细胞内及线粒体活性氧（reactive oxygen species，ROS）、线粒体膜电位和线粒体膜孔通透性；采用Western blotting和qRT-PCR检测电压依赖性阴离子通道1（voltage-dependent anion channel 1，VDAC1）、p53、PI3K/Akt/GSK-3β信号通路及核转录因子E2相关因子（nuclear factor E2-related factor 2，Nrf2）/血红素加氧酶-1（heme oxygenase-1，HO-1）通路相关蛋白及基因的表达。结果 粉防己碱可呈剂量相关性抑制MCF-7细胞增殖、迁移、侵袭并诱导细胞凋亡（P ＜0.01），升高细胞及线粒体内ROS、MDA和Fe²⁺水平（P ＜0.01），加剧脂质过氧化，降低线粒体膜电位（P ＜0.01），提高线粒体膜孔通透性（P ＜0.01），上调HO-1、p53、GSK-3β和VDAC1的表达（P ＜0.01），下调Nrf2、PI3K、p-PI3K、Akt和p-Akt的表达（P ＜0.01）。敲低PRDX1 基因可显著增强粉防己碱的上述作用（P ＜0.01）。结论 粉防己碱通过调控PI3K/Akt/GSK-3β信号通路诱导MCF-7细胞发生线粒体相关性铁死亡，且PRDX1在其中发挥关键的调控作用。, authors=辛国松1,2 , 孙越1 , 刘禹含1 , 侯妍秀1 , 李海茹3 , authorsList=辛国松, 孙越, 刘禹含, 侯妍秀, 李海茹, authorCompany=1 哈尔滨商业大学 药物工程技术研究中心, 黑龙江 哈尔滨 150076; et al . Tetrandrine alleviates pulmonary fibrosis by inhibiting alveolar epithelial cell senescence through PINK1/Parkin-mediated mitophagy [J]. Eur J Pharmacol , 2024, 969: 176459.et al . Tetrandrine improves ventricular remodeling and inflammation via inhibition of the MAPK/NF-κB pathway [J]. Int Heart J , 2025, 66(3): 463-474.et al . Tetrandrine inhibits aldosterone synthesis by covalently targeting CYP11A1 to attenuate hypertension [J]. Front Pharmacol , 2024, 15: 1387756.et al . The regulation of ferroptosis by tumor suppressor p53 and its pathway [J]. Int J Mol Sci , 2020, 21(21): 8387.J Cell Signal , 2020, 1(4): 155-159.
中草药
|药理与临床
2026
, 57
(13) :
5120
-5129
基于过氧化物还原酶1介导的粉防己碱调控乳腺癌细胞铁死亡机制研究
全屏
辛国松1,2 , 孙越1 , 刘禹含1 , 侯妍秀1 , 李海茹3
作者信息
1 哈尔滨商业大学 药物工程技术研究中心, 黑龙江 哈尔滨 150076;
通讯作者:
李海茹
作者简介:
辛国松: 辛国松（1984—），男，研究员，博士（博士后），研究方向为抗肿瘤中药药理学研究。E-mail:13766801150@163.com
Mechanism of tetrandrine regulating ferroptosis of breast cancer cells mediated by peroxiredoxin 1
XIN Guosong, SUN Yue, LIU Yuhan, HOU Yanxiu, LI Hairu
Affiliations
doi: 10.7501/j.issn.0253-2670.2026.13.015
文章导航
目的 基于过氧化物还原酶1（peroxiredoxin 1，PRDX1）研究粉防己碱调控磷脂酰肌醇3-激酶（phosphatidylinositol 3-kinase，PI3K）/蛋白激酶B（protein kinase B，Akt）/糖原合成激酶-3β（glycogen synthesizing kinase-3β，GSK-3β）信号通路诱导乳腺癌细胞铁死亡的作用机制。方法 人乳腺癌MCF-7细胞转染PRDX1 siRNA后，给予粉防己碱干预，考察粉防己碱对MCF-7细胞增殖、凋亡、迁移和侵袭能力的影响；检测丙二醛（malondialdehyde，MDA）、Fe²⁺、细胞内及线粒体活性氧（reactive oxygen species，ROS）、线粒体膜电位和线粒体膜孔通透性；采用Western blotting和qRT-PCR检测电压依赖性阴离子通道1（voltage-dependent anion channel 1，VDAC1）、p53、PI3K/Akt/GSK-3β信号通路及核转录因子E2相关因子（nuclear factor E2-related factor 2，Nrf2）/血红素加氧酶-1（heme oxygenase-1，HO-1）通路相关蛋白及基因的表达。结果 粉防己碱可呈剂量相关性抑制MCF-7细胞增殖、迁移、侵袭并诱导细胞凋亡（P ＜0.01），升高细胞及线粒体内ROS、MDA和Fe²⁺水平（P ＜0.01），加剧脂质过氧化，降低线粒体膜电位（P ＜0.01），提高线粒体膜孔通透性（P ＜0.01），上调HO-1、p53、GSK-3β和VDAC1的表达（P ＜0.01），下调Nrf2、PI3K、p-PI3K、Akt和p-Akt的表达（P ＜0.01）。敲低PRDX1 基因可显著增强粉防己碱的上述作用（P ＜0.01）。结论 粉防己碱通过调控PI3K/Akt/GSK-3β信号通路诱导MCF-7细胞发生线粒体相关性铁死亡，且PRDX1在其中发挥关键的调控作用。
粉防己碱
/
PRDX1
/
乳腺癌
/
铁死亡
/
PI3K/Akt/GSK-3β信号通路
Objective To investigate the mechanism by which tetrandrine regulates the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/glycogen synthase kinase-3β (GSK-3β) signaling pathway to induce ferroptosis in breast cancer cells based on peroxiredoxin 1 (PRDX1). Methods Human breast cancer MCF-7 cells transfected with PRDX1 siRNA were treated with tetrandrine to examine its effects on cell proliferation, apoptosis, migration and invasion. The levels of malondialdehyde (MDA), Fe2+ , intracellular and mitochondrial reactive oxygen species (ROS), mitochondrial membrane potential, and mitochondrial membrane permeability were measured. Western blotting and qRT-PCR were used to detect the expressions of voltage-dependent anion channel 1 (VDAC1), p53, PI3K/Akt/GSK-3β signaling pathway and nuclear factor E2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway related proteins and genes. Results Tetrandrine dose-dependently inhibited MCF-7 cell proliferation, migration and invasion while inducing apoptosis (P < 0.01), increased intracellular and mitochondrial ROS, MDA and Fe2+ levels (P < 0.01), aggravated lipid peroxidation, reduced mitochondrial membrane potential (P < 0.01), enhanced mitochondrial membrane permeability (P < 0.01), upregulated HO-1, p53, GSK-3β and VDAC1 expressions (P < 0.01), downregulated Nrf2, PI3K, p-PI3K, Akt and p-Akt expressions (P < 0.01). Knockdown of PRDX1 gene significantly amplified these effects of tetrandrine (P < 0.01). Conclusion Tetrandrine induces mitochondria-associated ferroptosis in MCF-7 cells via PI3K/Akt/GSK-3β signaling pathway, with PRDX1 playing a critical regulatory role.
tetrandrine
/
PRDX1
/
breast cancer
/
ferroptosis
/
PI3K/Akt/GSK-3β signaling pathway
辛国松, 孙越, 刘禹含, 侯妍秀, 李海茹.
基于过氧化物还原酶1介导的粉防己碱调控乳腺癌细胞铁死亡机制研究.
中草药,
2026
, 57
(13)
: 5120
-5129
.
DOI: 10.7501/j.issn.0253-2670.2026.13.015
XIN Guosong, SUN Yue, LIU Yuhan, HOU Yanxiu, LI Hairu.
Mechanism of tetrandrine regulating ferroptosis of breast cancer cells mediated by peroxiredoxin 1[J].
Chinese Traditional and Herbal Drugs ,
2026
, 57
(13)
: 5120
-5129
.
DOI: 10.7501/j.issn.0253-2670.2026.13.015
黑龙江省自然科学基金项目 (PL2025H208); 2023年度哈尔滨商业大学镜湖学者支持计划; 哈尔滨市科技计划自筹经费项目 (2023ZCZJCG038); 黑龙江省中医药科研项目 (ZHY2024-239); 2025年度黑龙江省省属本科高校基本科研业务费项目资助 (2025-KYYWF-ZR0101)
参考文献
引证文献
邬昊, 吕青. 全球及中国乳腺癌的流行病学趋势及防控启示: 2018—2022年《全球癌症统计报告》解读 [J]. 中国普外基础与临床杂志, 2024, 31(7): 796-802.et al . Tetrandrine alleviates pulmonary fibrosis by inhibiting alveolar epithelial cell senescence through PINK1/Parkin-mediated mitophagy [J]. Eur J Pharmacol , 2024, 969: 176459.et al . Tetrandrine improves ventricular remodeling and inflammation via inhibition of the MAPK/NF-κB pathway [J]. Int Heart J , 2025, 66(3): 463-474.et al . Tetrandrine inhibits aldosterone synthesis by covalently targeting CYP11A1 to attenuate hypertension [J]. Front Pharmacol , 2024, 15: 1387756.et al . The regulation of ferroptosis by tumor suppressor p53 and its pathway [J]. Int J Mol Sci , 2020, 21(21): 8387.J Cell Signal , 2020, 1(4): 155-159.
2026年第57卷第13期
PDF下载
22
5
引用本文
BibTeX
文章信息
doi: 10.7501/j.issn.0253-2670.2026.13.015
接收时间：2026-01-08
首发时间：2026-09-09
https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.13.015
引用本文
BibTeX
2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
关闭全屏
BibTeX
EndNote
RefWorks
TxT
PDF下载
全屏
22
5
关闭全屏
北京市海淀区学院南路86号
010-62199257（仅加盟咨询）
qkjq@cast.org.cn
