Article(id=1304388163833720924, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388049975137100, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.11.023, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1764604800000, receivedDateStr=2025-12-02, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788919972618, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788919972618, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788919972618, creator=13701087609, updateTime=1788919972618, updator=13701087609, issue=Issue{id=1304388049975137100, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='11', pageStart='4089', pageEnd='4508', issueExtLink='null', onlineDate='null', pubDate='1781193600000', pubDateStr='2026-06-12', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788919945471, creator='13701087609', updateTime=1788923432386, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304402675202805770, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388049975137100, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304402675207000075, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388049975137100, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=4364, endPage=4375, ext={EN=ArticleExt(id=1304388165284950111, articleId=1304388163833720924, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Screening of phosphate-solubilizing bacteria from rhizosphere of Fritillaria taipaiensis in different habitats and its inoculation effect, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective To screen high-efficiency phosphate-solubilizing bacteria (PSB) in the rhizosphere soil of Fritillaria taipaiensis and provide data support for the development of microbial fertilizers. Methods Rhizosphere soil samples were collected from ten production areas. Strains of inorganic phosphate-solubilizing bacteria (IPSB) and organic phosphate-solubilizing bacteria (OPSB) were isolated and purified. Their phosphate-solubilizing capacities were evaluated qualitatively and quantitatively, and the strains were identified based on physiological and biochemical characteristics as well as 16S rDNA sequencing. The effects of selected strains were further verified using pot experiments. Results A total of 42 IPSB and 31 OPSB strains were isolated. Quantitative analysis showed that among the IPSB strains, WP2-2 exhibited the strongest solubilizing capacity, increasing available phosphorus by 231.30 mg/L, followed by WP7-2 (211.05 mg/L). Among the OPSB strains, YP3-1 showed the highest phosphorus solubilization (28.57 mg/L), followed by YP5-1 (22.30 mg/L). Strain WP2-2 was identified as Serratia plymuthica, WP7-2 as Bacillus cereus, and both YP3-1 and YP5-1 were also identified as B. cereus. Pot experiments revealed that inoculation with these PSB strains altered the total phosphorus content in bulbs and fibrous roots, and also influenced the total alkaloid content in the bulbs. Conclusion The strains WP2-2, WP7-2, YP3-1, and YP5-1 show great potential for developing specialized microbial fertilizers for the cultivation of F. taipaiensis., authors=WANG Kai, SHI Zhifen, WANG Panpan, WANG Guangzhi, MA Qiang, ZHOU Nong, authorsList=WANG Kai, SHI Zhifen, WANG Panpan, WANG Guangzhi, MA Qiang, ZHOU Nong, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304388165175898206, articleId=1304388163833720924, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=不同生长环境下太白贝母根际解磷细菌的筛选及其接种效应研究, columnId=1304140203967410414, journalTitle=中草药, columnName=药材与资源, runingTitle=null, highlight=null, articleAbstract=目的 筛选太白贝母Fritillaria taipaiensis根际土壤高效解磷细菌,为微生物菌肥的有效开发提供数据支撑。方法 从10个产地的太白贝母根际土壤中筛选和鉴定解无机磷细菌和解有机磷细菌,经分离、纯化以及解磷能力的定性与定量分析,并结合生理生化和16S rDNA技术鉴定菌株,之后通过盆栽实验测定其接种效果。结果 最终从10个不同产地的太白贝母根际土壤中筛选得到42株解无机磷细菌和31株解有机磷细菌。解磷能力定量结果显示,解无机磷细菌中菌株WP2-2表现出最强的解磷能力,增磷量为231.30 mg/L,其次是菌株WP7-2,增磷量为211.05 mg/L。解有机磷细菌中菌株YP3-1的解磷能力最强,增磷量为28.57 mg/L,菌株YP5-1次之,增磷量为22.30 mg/L。经鉴定,WP2-2为普城沙雷氏菌Serratia plymuthica,WP7-2是蜡状芽孢杆菌Bacillus cereus,YP3-1和YP5-1同属蜡状芽孢杆菌B. cereus。盆栽试验结果显示,接种解磷细菌能够改变太白贝母鳞茎和须根的全磷含量,在一定程度上影响鳞茎总生物碱含量。结论 菌株WP2-2、WP7-2、YP3-1、YP5-1可作为太白贝母根际微生物菌肥开发的候选菌株,为后续需解磷细菌与有效成分相关基因调控之间的研究提供了理论基础。, authors=王凯1,2, 施志芬1,2,3, 王盼盼2, 王光志1, 马强4, 周浓2, authorsList=王凯, 施志芬, 王盼盼, 王光志, 马强, 周浓, authorCompany=1 成都中医药大学药学院, 四川 成都 611130;
2 重庆三峡科技大学生物与食品工程学院, 三峡库区道地药材绿色种植与深加工重庆市工程研究中心, 重庆 404020;
3 红河职业技术学院 现代农业学院, 云南 红河 661199;
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乔志伟, 洪坚平, 方曌, 等. 溶磷细菌对复垦土壤有效磷及各形态无机磷含量的影响[J]. 河南农业科学, 2017, 46(9): 50-55.
蔺宝珺, 杨文权, 赵帅, 等. 高寒草甸植物根际溶磷菌的筛选鉴定及其溶磷与促生效果[J]. 草地学报, 2022, 30(11): 3132-3139.
东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001: 374-390.
韩雪娇. 杨树根际土壤解磷细菌的筛选、解磷特性及其油菜促生效应研究[D]. 淮阴: 淮阴工学院, 2020.
李豆豆, 尚双华, 韩巍, 等. 一株高效解磷真菌新菌株的筛选鉴定及解磷特性[J]. 应用生态学报, 2019, 30(7): 2384-2392.
骆韵涵, 柯志滨, 钟超, 等. 红树林土壤解磷菌的分离鉴定及解磷特性[J]. 中国环境科学, 2020, 40(6): 2664-2673.
晋婷婷, 任嘉红, 刘瑞祥. 南方红豆杉根际解有机磷细菌的鉴定及其解磷特性和促生作用研究[J]. 西北植物学报, 2016, 36(9): 1819-1827.
曹冠华, 张雪, 马诗婷, 等. 阳春砂仁根内生真菌解磷功能评价及分类学鉴定[J]. 中草药, 2020, 51(5): 1316-1323.
Shi Z F, Pan F M, Kong X T, et al. Effects of inoculation with phosphate solubilizing bacteria on the physiology, biochemistry, and expression of genes related to the protective enzyme system of Fritillaria taipaiensis P. Y. Li [J]. Phyton, 2024, 93(2): 247-260.
中国药典[S]. 一部. 2025: 38.
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吴凡, 崔萍, 夏尚远, 等. 桑树根际解磷细菌的分离鉴定及解磷能力测定[J]. 蚕业科学, 2007, 33(4): 521-527.
朱德旋, 杜春梅, 董锡文, 等. 一株寒地高效解无机磷细菌的分离鉴定及拮抗作用[J]. 微生物学报, 2020, 60(8): 1672-1682.
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李宁, 王珊珊, 马丽丽, 等. 两株高效溶磷菌的溶磷能力及其对玉米生长和红壤磷素形态的影响[J]. 植物营养与肥料学报, 2021, 27(2): 275-283.
陈倩颖. 解有机磷细菌的分离鉴定及其解磷特性研究[D]. 合肥: 安徽农业大学, 2009.
杨慧, 范丙全, 龚明波, 等. 一株新的溶磷草生欧文氏菌的分离、鉴定及其溶磷效果的初步研究[J]. 微生物学报, 2008, 48(1): 51-56.
Halder A K, Chakrabartty P K. Solubilization of inorganic phosphate by Rhizobium [J]. Folia Microbiol, 1993, 38(4): 325-330.
李卓蔚, 郎佳琪, 孟琦, 等. 接种解有机磷细菌对滇重楼品质及土壤肥力的影响[J]. 中国实验方剂学杂志, 2022, 28(16): 156-164.
赵晶晶, 郭冬琴, 杨敏, 等. 不同剂量解磷菌对滇重楼生长发育的影响[J]. 环境化学, 2022, 41(2): 761-769.
张建海, 冯彬彬, 吴翠色. 丛枝菌根真菌对太白贝母生长及质量标志物的影响[J]. 中国中医药信息杂志, 2020, 27(7): 88-93.
Araque Gelves D I, Andreoli de Souza G C, Salvador M J, et al. Impact of plant-microorganism interaction: A key driver for the production of bioactive metabolites of interest in the pharmaceutical, agricultural, cosmetic, and food industries [J]. J Plant Growth Regul, 2025: 236-250.)
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不同生长环境下太白贝母根际解磷细菌的筛选及其接种效应研究
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中草药 |药材与资源 2026 , 57 (11) : 4364 -4375
不同生长环境下太白贝母根际解磷细菌的筛选及其接种效应研究
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王凯1,2, 施志芬1,2,3, 王盼盼2, 王光志1, 马强4, 周浓2
作者信息
    1 成都中医药大学药学院, 四川 成都 611130;
    2 重庆三峡科技大学生物与食品工程学院, 三峡库区道地药材绿色种植与深加工重庆市工程研究中心, 重庆 404020;
    3 红河职业技术学院 现代农业学院, 云南 红河 661199;
    4 重庆三峡医药高等专科学校 基础医学部, 重庆 404120
通讯作者:
马强
作者简介:
王凯: 王凯,硕士研究生,研究方向为中药品种、质量与资源开发应用研究。E-mail:wangkai720607@163.com
Screening of phosphate-solubilizing bacteria from rhizosphere of Fritillaria taipaiensis in different habitats and its inoculation effect
  • WANG Kai, SHI Zhifen, WANG Panpan, WANG Guangzhi, MA Qiang, ZHOU Nong
  • Affiliations
    doi: 10.7501/j.issn.0253-2670.2026.11.023
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    目的 筛选太白贝母Fritillaria taipaiensis根际土壤高效解磷细菌,为微生物菌肥的有效开发提供数据支撑。方法 从10个产地的太白贝母根际土壤中筛选和鉴定解无机磷细菌和解有机磷细菌,经分离、纯化以及解磷能力的定性与定量分析,并结合生理生化和16S rDNA技术鉴定菌株,之后通过盆栽实验测定其接种效果。结果 最终从10个不同产地的太白贝母根际土壤中筛选得到42株解无机磷细菌和31株解有机磷细菌。解磷能力定量结果显示,解无机磷细菌中菌株WP2-2表现出最强的解磷能力,增磷量为231.30 mg/L,其次是菌株WP7-2,增磷量为211.05 mg/L。解有机磷细菌中菌株YP3-1的解磷能力最强,增磷量为28.57 mg/L,菌株YP5-1次之,增磷量为22.30 mg/L。经鉴定,WP2-2为普城沙雷氏菌Serratia plymuthica,WP7-2是蜡状芽孢杆菌Bacillus cereus,YP3-1和YP5-1同属蜡状芽孢杆菌B. cereus。盆栽试验结果显示,接种解磷细菌能够改变太白贝母鳞茎和须根的全磷含量,在一定程度上影响鳞茎总生物碱含量。结论 菌株WP2-2、WP7-2、YP3-1、YP5-1可作为太白贝母根际微生物菌肥开发的候选菌株,为后续需解磷细菌与有效成分相关基因调控之间的研究提供了理论基础。
    太白贝母  /  解磷细菌  /  根际土壤  /  分离鉴定  /  接种效应
    Objective To screen high-efficiency phosphate-solubilizing bacteria (PSB) in the rhizosphere soil of Fritillaria taipaiensis and provide data support for the development of microbial fertilizers. Methods Rhizosphere soil samples were collected from ten production areas. Strains of inorganic phosphate-solubilizing bacteria (IPSB) and organic phosphate-solubilizing bacteria (OPSB) were isolated and purified. Their phosphate-solubilizing capacities were evaluated qualitatively and quantitatively, and the strains were identified based on physiological and biochemical characteristics as well as 16S rDNA sequencing. The effects of selected strains were further verified using pot experiments. Results A total of 42 IPSB and 31 OPSB strains were isolated. Quantitative analysis showed that among the IPSB strains, WP2-2 exhibited the strongest solubilizing capacity, increasing available phosphorus by 231.30 mg/L, followed by WP7-2 (211.05 mg/L). Among the OPSB strains, YP3-1 showed the highest phosphorus solubilization (28.57 mg/L), followed by YP5-1 (22.30 mg/L). Strain WP2-2 was identified as Serratia plymuthica, WP7-2 as Bacillus cereus, and both YP3-1 and YP5-1 were also identified as B. cereus. Pot experiments revealed that inoculation with these PSB strains altered the total phosphorus content in bulbs and fibrous roots, and also influenced the total alkaloid content in the bulbs. Conclusion The strains WP2-2, WP7-2, YP3-1, and YP5-1 show great potential for developing specialized microbial fertilizers for the cultivation of F. taipaiensis.
    Fritillaria taipaiensis P. Y. Li  /  phosphate-solubilizing bacteria  /  rhizosphere soil  /  separation and identification  /  inoculation effect
    王凯, 施志芬, 王盼盼, 王光志, 马强, 周浓. 不同生长环境下太白贝母根际解磷细菌的筛选及其接种效应研究. 中草药, 2026 , 57 (11) : 4364 -4375 . DOI: 10.7501/j.issn.0253-2670.2026.11.023
    WANG Kai, SHI Zhifen, WANG Panpan, WANG Guangzhi, MA Qiang, ZHOU Nong. Screening of phosphate-solubilizing bacteria from rhizosphere of Fritillaria taipaiensis in different habitats and its inoculation effect[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (11) : 4364 -4375 . DOI: 10.7501/j.issn.0253-2670.2026.11.023

      重庆市自然科学基金面上项目 (CSTB2023NSCQ-LMX0010)

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    吴凡, 崔萍, 夏尚远, 等. 桑树根际解磷细菌的分离鉴定及解磷能力测定[J]. 蚕业科学, 2007, 33(4): 521-527.
    朱德旋, 杜春梅, 董锡文, 等. 一株寒地高效解无机磷细菌的分离鉴定及拮抗作用[J]. 微生物学报, 2020, 60(8): 1672-1682.
    郭艺鹏, 王海儒, 孙林琦, 等. 枣根际解磷细菌的分离筛选及16S rDNA鉴定[J]. 河南农业大学学报, 2015, 49(6): 811-816.
    李宁, 王珊珊, 马丽丽, 等. 两株高效溶磷菌的溶磷能力及其对玉米生长和红壤磷素形态的影响[J]. 植物营养与肥料学报, 2021, 27(2): 275-283.
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    李卓蔚, 郎佳琪, 孟琦, 等. 接种解有机磷细菌对滇重楼品质及土壤肥力的影响[J]. 中国实验方剂学杂志, 2022, 28(16): 156-164.
    赵晶晶, 郭冬琴, 杨敏, 等. 不同剂量解磷菌对滇重楼生长发育的影响[J]. 环境化学, 2022, 41(2): 761-769.
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    doi: 10.7501/j.issn.0253-2670.2026.11.023
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    2种不同金属材料的力学参数

    Family
    属数
    Number of
    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)

    Genus
    种数
    Number of
    species
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    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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