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不同光照强度对白芷根系形态及香豆素类成分积累的影响

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中草药 | 药材与资源 2026,57(12): 4823-4834

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中草药 |药材与资源 2026 , 57 (12) : 4823 -4834

不同光照强度对白芷根系形态及香豆素类成分积累的影响

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邢春琴^1,2, 吴清华^1,2,3, 张佳旭^1,2, 黄成凤^1,2, 杜金雨^1,2, 黄旭龙^1,2, 周涛^1,2, 裴瑾^1,2

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通讯作者:

吴清华

作者简介:

邢春琴: 邢春琴（2001—），女，硕士研究生。E-mail:xchunqin121@163.com

Effects of different light intensities on root morphology and accumulation of active ingredients in Angelica dahurica

XING Chunqin, WU Qinghua, ZHANG Jiaxu, HUANG Chengfeng, DU Jinyu, HUANG Xulong, ZHOU Tao, PEI Jin

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doi: 10.7501/j.issn.0253-2670.2026.12.024

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目的探讨不同光照强度处理不同发育时期的白芷Angelica dahurica根系形态建成与香豆素类成分积累之间的关系，为提升白芷药材品质及高效合理栽培生产提供参考和依据。方法测定50%遮阴、自然光、补光处理组白芷根系形态指标；采用HPLC法和相关性分析，测定并筛选与白芷根系形态建成关联性较强的香豆素类成分；通过施加外源香豆素，观测白芷根系形态，基于RT-qPCR技术测定其根系香豆素生物合成相关基因表达水平。结果白芷根系形态指标（主根干质量、侧根数量、主根直径、根冠比）与光照强度呈正相关；不同光强处理组总香豆素含量整体变化呈现“S”型曲线。伞形花内酯与主根长、侧根数、根冠比呈显著负相关，与主根直径呈显著正相关。外源施加伞形花内酯可显著促进白芷根系形态参数（总根表面积、总根长和根分支数）和4个香豆素（氧化前胡素、欧前胡素、珊瑚菜素、异欧前胡素）含量增加，并上调其香豆素生物合成途径上的苯丙氨酸氨酰化酶（phenylalanine ammonia-lyase，PAL）、肉桂酸-4-羟化酶（cinnamate-4-hydroxylase，C4H）、4-香豆素-CoA连接酶（4-coumarate-CoA ligase，4CL）等关键基因的表达量。结论白芷在不同光强、不同发育时期根系指标和香豆素含量存在差异，伞形花内酯外源施加会影响白芷根系建成，且通过调控白芷根中香豆素合成途径相关基因表达，影响白芷根中香豆素类成分含量的积累。

关键词

白芷 / 光照强度 / 根系发育 / HPLC / 香豆素积累 / 伞形花内酯 / RT-qPCR / 氧化前胡素 / 欧前胡素 / 珊瑚菜素 / 异欧前胡素

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Objective In order to study the relationship between the morphology of Angelica dahurica root system and the accumulation of coumarin-like components at different developmental stages under different light intensity treatments, to provide reference and basis for improving the quality of A. dahurica herbs and for efficient and reasonable cultivation and production. Methods The morphological indexes of A. dahurica root system were determined in 50% shade, natural light, and supplemental light treatment groups; High-performance liquid chromatography (HPLC) and correlation analysis were used to determine and screen the coumarin-like components that were strongly associated with the morphology of A. dahurica root system; The morphology of the root system of A. dahurica was observed by the application of exogenous coumarin, and the expression level of coumarin biosynthesis-related genes in the root system was determined based on the RT-qPCR technique. Results Root morphology indexes of A. dahurica (dry weight of primary root, number of lateral roots, diameter of primary root, root-crown ratio) were positively correlated with light intensity; the overall change of the total coumarin content in the different light intensity treatment groups showed an “S” - shaped curve. Umbelliferolide was significantly negatively correlated with primary root length, number of lateral roots, and root-crown ratio, and significantly positively correlated with primary root diameter; exogenous application of umbelliferolide significantly promoted the increase of the morphological parameters of A. dahurica root system (total surface area of the root, the length of the total root, and the number of root branches), and the content of the four complex coumarins (oxypeucedanin, imperatorin, phellopterin, and isoimperatorin), and significantly up-regulated the expression of key genes of coumarin biosynthesis pathway, including phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate-CoA ligase (4CL) and other key genes expression. Conclusion A. dahurica root indexes and coumarin content varied in different light intensities and developmental periods. The exogenous application of umbelliferolide affected the establishment of dahurica root system and the accumulation of coumarin-like components in dahurica roots by regulating the expression of genes related to the coumarin synthesis pathway in dahurica roots.

Key words

Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. / light intensity / root development / HPLC / coumarin accumulation / umbelliferone / RT-qPCR / oxypeucedanin / imperatorin / phellopterin / isoimperatorin

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邢春琴, 吴清华, 张佳旭, 黄成凤, 杜金雨, 黄旭龙, 周涛, 裴瑾. 不同光照强度对白芷根系形态及香豆素类成分积累的影响. 中草药, 2026 , 57 (12) : 4823 -4834 . DOI: 10.7501/j.issn.0253-2670.2026.12.024

XING Chunqin, WU Qinghua, ZHANG Jiaxu, HUANG Chengfeng, DU Jinyu, HUANG Xulong, ZHOU Tao, PEI Jin. Effects of different light intensities on root morphology and accumulation of active ingredients in Angelica dahurica[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (12) : 4823 -4834 . DOI: 10.7501/j.issn.0253-2670.2026.12.024

基金

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四川省科技计划重点研发项目 (2023YFS0338); 四川省科技厅重点研发计划（重大科技专项）社会发展领域重点研发项目 (2022YFS0582); 国家中医药管理局中医药创新团队及人才支持计划项目 (ZYYCXTD-D-202209); 成都中医药大学杏林学者青基人才专项 (QJRC2022034); 中央本级重大增减支项目名贵中药资源可持续利用能力建设项目” (2060302)

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2026年第57卷第12期

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doi: 10.7501/j.issn.0253-2670.2026.12.024

接收时间：2026-01-02
首发时间：2026-09-09

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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