中草药

红花CtFDs基因的克隆及异源表达分析

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于丽莉, 黎继华, 张煜茹, 赵虹, 李校堃, 张健, 刘秀明

中草药 | 药材与资源 2026,57(10): 3954-3962

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中草药 |药材与资源 2026 , 57 (10) : 3954 -3962

红花CtFDs基因的克隆及异源表达分析

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于丽莉, 黎继华, 张煜茹, 赵虹, 李校堃, 张健, 刘秀明

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通讯作者:

张健

作者简介:

于丽莉: 于丽莉(1998—),女,博士研究生,研究方向为红花活性成分代谢调控。E-mail:15640309810@163.com

Cloning and heterologous expression analysis of CtFDs gene from Carthamus tinctorius

YU Lili, LI Jihua, ZHANG Yuru, ZHAO Hong, LI Xiaokun, ZHANG Jian, LIU Xiuming

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doi: 10.7501/j.issn.0253-2670.2026.10.023

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目的从红花Carthamus tinctorius基因组中克隆CtFD1与CtFD2基因，对其进行生物信息学、表达特异性和亚细胞定位分析。方法基于红花基因组筛选并克隆CtFD1与CtFD2基因，利用生物信息学在线网站进行理化性质、保守结构域等分析；通过实时荧光定量PCR（quantitative real-time polymerase chain reaction，qRT-PCR）检测其组织表达特性；利用烟草瞬时表达系统检测观察亚细胞定位情况；通过优化诱导剂浓度及温度使蛋白为可溶性表达。结果 CtFD1与CtFD2基因CDS大小分别为726 bp和540 bp，编码241和179个氨基酸。二者均包含典型的bZIP转录因子结合域，为亲水性蛋白，定位于细胞核。系统进化树显示与黄花蒿Artemisia annua亲缘关系较近。组织表达分析表明，CtFD1与CtFD2基因在茎尖分生组织（shoot apical meristem，SAM）中表达量最高。原核表达分析结果显示在16 ℃、IPTG浓度为0.2 mmol/L时，蛋白可在上清中大量表达。结论 CtFD1与CtFD2基因属于bZIP家族成员，在红花茎尖分生组织中表达量最高，表明这2个基因对红花开花调控可能发挥重要作用，为进一步研究基因功能及开花机制解析提供了科学依据。

关键词

红花 / CtFDs基因 / 基因克隆 / 生物信息学 / 表达分析

Abstract

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Objective To clone the CtFD1 and CtFD2 genes from the genome of Carthamus tinctorius and perform bioinformatics, expression specificity, and subcellular localization analyses. Methods The CtFD1 and CtFD2 genes were screened and cloned based on the C. tinctorius genome. Bioinformatics online tools were used to analyze physicochemical properties, conserved domains, and other features. Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to examine tissue-specific expression patterns. Subcellular localization was observed using a tobacco transient expression system. Soluble expression of the proteins was achieved by optimizing inducer concentration and temperature. Results The coding sequences (CDS) of CtFD1 and CtFD2 were 726 bp and 540 bp in length, encoding 241 and 179 amino acids, respectively. Both proteins contained a typical bZIP transcription factor binding domain, which were hydrophilic, and localized to the nucleus. Phylogenetic analysis indicated a close evolutionary relationship with Artemisia annua. Tissue expression analysis showed that CtFD1 and CtFD2 were most highly expressed in shoot apical meristem (SAM). Prokaryotic expression analysis revealed that both proteins were abundantly expressed in the supernatant at 16  ℃ with an IPTG concentration of 0.2 mmol/L. Conclusion The CtFD1 and CtFD2 genes belong to the bZIP family and exhibit the highest expression levels in the SAM of C. tinctorius, suggesting that they may play important roles in regulating flowering. This study provides a scientific basis for further functional characterization of these genes and elucidation of the flowering mechanism in C. tinctorius.

Key words

Carthamus tinctorius L. / CtFDs gene / gene cloning / bioinformatics analysis / expression analysis

引用本文

于丽莉, 黎继华, 张煜茹, 赵虹, 李校堃, 张健, 刘秀明. 红花CtFDs基因的克隆及异源表达分析. 中草药, 2026 , 57 (10) : 3954 -3962 . DOI: 10.7501/j.issn.0253-2670.2026.10.023

YU Lili, LI Jihua, ZHANG Yuru, ZHAO Hong, LI Xiaokun, ZHANG Jian, LIU Xiuming. Cloning and heterologous expression analysis of CtFDs gene from Carthamus tinctorius[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (10) : 3954 -3962 . DOI: 10.7501/j.issn.0253-2670.2026.10.023

基金

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兵团自然科学支持计划重点项目 (2025DA003); 新疆院士人才发展基金

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2026年第57卷第10期

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doi: 10.7501/j.issn.0253-2670.2026.10.023

接收时间：2026-02-02
首发时间：2026-09-09

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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