Article(id=1304140207209607438, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304140186485543391, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.03.022, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1759334400000, receivedDateStr=2025-10-02, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788860855151, onlineDateStr=2026-09-08, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788860855151, onlineIssueDateStr=2026-09-08, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788860855151, creator=13701087609, updateTime=1788860855151, updator=13701087609, issue=Issue{id=1304140186485543391, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='3', pageStart='789', pageEnd='1208', issueExtLink='null', onlineDate='null', pubDate='1770825600000', pubDateStr='2026-02-12', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788860850211, creator='13701087609', updateTime=1788860942564, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304140573955351430, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304140186485543391, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304140573955351431, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304140186485543391, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1054, endPage=1063, ext={EN=ArticleExt(id=1304140207540957456, articleId=1304140207209607438, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Bioinformatics and expression analysis of SmABCG34 gene in Salvia miltiorrhiza, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective To clone the SmABCG34 gene from the Salvia miltiorrhiza genome and analyze its bioinformatics and expression characteristics. Methods The SmABCG34 gene was cloned from the S. miltiorrhiza genome, and the Conservative Domain and physicochemical properties of the gene and its encoded protein were analyzed by Bioinformatics Online Website. Quantitative real-time PCR (qRT-PCR) was used to detect the tissue expression specificity of SmABCG34 gene and its response to stress induced by abscisic acid (ABA), trans-zeatin (tZ), and polyethylene glycol-6000 (PEG-6000). Results The CDS of the SmABCG34 gene is 4 347 bp in size and encodes 1 448 amino acids; This protein contains a typical NBD-TMD-NBD-TMD structure and belongs to the full-molecule transporter of the ABCG subfamily; subcellular localization shows that this protein was localizted to the cell membrane. Phylogenetic analysis showed that SmABCG34 was closely related to S. hispanica, with a protein homology of up to 91.23 %. The expression pattern analysis revealed that the SmABCG34 gene was expressed in the roots, stems, leaves and flowers of S. miltiorrhiza with the highest expression in the flowers, followed by the leaves. After treatment with ABA, tZ, and PEG-6000, compared to 0 h, the expression of the SmABCG34 gene was significantly upregulated at 12 h. Conclusion The SmABCG34 gene belongs to the ABCG subfamily and shows a significant response to hormone and stress treatments, providing a scientific basis for further exploration of the function of the SmABCG34 gene., authors=LIN Yanan, BAI Yanhong, QIU Xinran, ZU Xiuli, YU Juan, LIU Hongyan, GONG Yanan, CHEN Xue, LIU Qian, authorsList=LIN Yanan, BAI Yanhong, QIU Xinran, ZU Xiuli, YU Juan, LIU Hongyan, GONG Yanan, CHEN Xue, LIU Qian, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304140207444488463, articleId=1304140207209607438, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=丹参SmABCG34基因的生物信息学与表达分析, columnId=1304140203967410414, journalTitle=中草药, columnName=药材与资源, runingTitle=null, highlight=null, articleAbstract=目的 从丹参Salvia miltiorrhiza基因组中克隆SmABCG34基因，对其进行生物信息学和表达特异性分析。方法 基于丹参基因组筛选克隆SmABCG34基因，利用生物信息学在线网站对该基因及其编码蛋白进行理化性质、保守结构域等分析。利用实时荧光定量PCR（qRT-PCR）检测SmABCG34基因的组织表达特性及对脱落酸（abscisic acid，ABA）、反式玉米素（trans-Zeatin，tZ）、聚乙二醇-6000（polyethylene glycol-6000，PEG-6000）胁迫的响应情况。结果SmABCG34基因CDS大小为4 347 bp，编码1 448个氨基酸；该蛋白包含典型的结合域（nucleotide-binding domain，NBD）-跨膜结构域（transmembrane domain，TMD）-NBD-TMD结构，属于ABCG亚家族全分子转运蛋白；亚细胞定位预测显示该蛋白定位于细胞膜。系统进化分析表明，SmABCG34与西班牙鼠尾草Salvia hispanica亲缘关系较近，蛋白同源性高达91.23%。表达模式分析显示，SmABCG34基因在丹参根、茎、叶、花中均有表达，在花中表达量最高，叶中次之。ABA、tZ、PEG-6000处理后，相比于0 h，SmABCG34基因在12 h时表达均显著上调。结论SmABCG34基因属于ABCG亚家族成员，对激素和胁迫处理具有明显的响应，为后续探究SmABCG34基因的功能提供了科学依据。, authors=林亚楠1, 白艳红1, 邱馨冉1, 祖秀丽1, 于娟1, 刘红燕2,3,4, 宫亚楠5, 陈雪1,2,3, 刘谦1,2,3, authorsList=林亚楠, 白艳红, 邱馨冉, 祖秀丽, 于娟, 刘红燕, 宫亚楠, 陈雪, 刘谦, authorCompany=1 山东中医药大学药学院, 山东 济南 250300; 2 山东中医药大学, 中医药经典理论教育部重点实验室, 山东 济南 250300; 3 山东中医药大学, 山东省数字中药重点实验室, 山东 济南 250300; 4 山东中医药大学实验中心, 山东 济南 250300; 5 济南市章丘区人民医院心内一科, 山东 济南 250200, correspAuthors=陈雪, authorNote=林亚楠: 林亚楠（1999—），女，山东东营人，硕士研究生，研究方向为中药资源学。E-mail:18754621424@163.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=64X+WWcT5dnOGNUn1rW+fA==, pdfFileSize=2441127, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金资助(82404791);山东省自然科学基金项目(ZR2021QH202);山东省自然科学基金项目(ZR2023MH338);国家现代农业产业技术体系资助项目(CARS-21);菏泽市黄河流域生态保护和高质量发展科技创新突破计划项目(KJTP202308))}, authors=null, keywords=[Keyword(id=1304140207683563793, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304140207209607438, 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provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.03.022, detailUrlEn=https://castjournals.cast.org.cn/joweb/zcy/EN/10.7501/j.issn.0253-2670.2026.03.022, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zcy/CN/PDF/10.7501/j.issn.0253-2670.2026.03.022, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zcy/EN/PDF/10.7501/j.issn.0253-2670.2026.03.022, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1788860855151, fullTextJson=null, articleText=null, reference=中国药典 [S]. 一部. 2020: 77. 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Phytochemistry, 2021, 184: 112663. Kotlyarov S, Kotlyarova A. Biological Functions and Clinical Significance of the ABCG1 Transporter [J]. Biology (Basel), 2024, 14(1): 8. Yan L, Zhang J H, Chen H Y, et al. Genome-wide analysis of ATP-binding cassette transporter provides insight to genes related to bioactive metabolite transportation in Salvia miltiorrhiza [J]. BMC Genomics, 2021, 22(1): 315-315. Huo X H, Pan A, Lei M Y, et al. Genome-wide characterization and functional analysis of ABCG subfamily reveal its role in cutin formation in cotton [J]. Int J Mol Sci, 2023, 24(3): 2379. 孙洁婷, 敬雪皎, 赵丹妮, 等. 植物ABCG转运蛋白功能的研究进展 [J]. 科学通报, 2024, 69(14): 1866-1880. Liu L P, Zhao L H, Chen P J, et al. ATP binding cassette transporters ABCG1 and ABCG16 affect reproductive development via auxin signalling in Arabidopsis [J]. Plant J, 2020, 102(6): 1172-1186. Wang Y Y, Wang Y, Bai H F, et al. An ABCG-type transporter facilitates ABA influx and regulates camptothecin biosynthesis in Camptotheca acuminata [J]. Int J Mol Sci, 2022, 23(24): 16120. Li H, Li Z Y, Yang K B, et al. Comparison analysis of ABCG subfamily in bamboo and the potential function of PeABCG15 in monolignol transport [J]. Plant Physiol Biochem, 2024, 217: 109278. Banasiak J, Borghi L, Stec N, et al. The full-size ABCG transporter of Medicago truncatula is involved in strigolactone secretion, affecting arbuscular mycorrhiza [J]. Front Plant Sci, 2020, 11: 18. Zhao J Z, Yu N N, Ju M, et al. ABC transporter OsABCG18 controls the shootward transport of cytokinins and grain yield in rice [J]. J Exp Bot, 2019, 70(21): 6277-6291. Li Y J, Chen J F, Zhi J Y, et al. The ABC transporter SmABCG1 mediates tanshinones export from the peridermic cells of Salvia miltiorrhiza root [J]. J Integr Plant Biol, 2025, 67(1): 135-149. Schmittgen T D, Livak K J. Analyzing real-time PCR data by the comparative C(T) method [J]. Nat Protoc, 2008, 3(6): 1101-1108. Xiong J, Feng J M, Yuan D X, et al. Tracing the structural evolution of eukaryotic ATP binding cassette transporter superfamily [J]. Sci Rep, 2015, 5: 16724. 王梦龙, 翁灵灵, 吴淑英, 等. ABCG转运蛋白参与植物雄性育性调控的研究进展 [J]. 植物科学学报, 2024, 42(3): 387-394. Crouzet J, Roland J, Peeters E, et al. NtPDR1, a plasma membrane ABC transporter from Nicotiana tabacum, is involved in diterpene transport [J]. Plant Mol Biol, 2013, 82(1/2): 181-192. 何倩. 青蒿转运蛋白基因AaPDR12的克隆与功能分析 [D]. 上海: 上海交通大学, 2018. 李雅静. 丹参根中丹参酮类成分生物合成调控、转运机制及生理活性研究 [D]. 上海: 中国人民解放军海军军医大学, 2022. 李彤. 大豆GmABCG5L基因铁转运的功能研究 [D]. 哈尔滨: 哈尔滨师范大学, 2023. Lee E J, Kim K Y, Zhang J, et al. Arabidopsis seedling establishment under waterlogging requires ABCG5-mediated formation of a dense cuticle layer [J]. New Phytol, 2021, 229(1): 156-172. Jarzyniak K, Banasiak J, Jamruszka T, et al. Early stages of legume-rhizobia symbiosis are controlled by ABCG-mediated transport of active cytokinins [J]. Nat Plants, 2021, 7(4): 428-436. Adebesin F, Widhalm JR, Boachon B, et al. Emission of volatile organic compounds from petunia flowers is facilitated by an ABC transporter [J]. Science, 2017, 356(6345): 1386-1388. 董润鑫, 徐芯, 李彦妮, 等. 丹参花总酚酸和花青素的含量测定 [J]. 四川农业科技, 2024, 43(9): 70-75. Reshi ZA, Ahmad W, Lukatkin AS, et al. From Nature to Lab: A Review of Secondary Metabolite Biosynthetic Pathways, Environmental Influences, and In Vitro Approaches [J]. Metabolites, 2023, 13(8): 895. Gai Z, Wang Y, Ding Y, et al. Exogenous abscisic acid induces the lipid and flavonoid metabolism of tea plants under drought stress [J]. Sci Rep, 2020, 10(1): 12275. Fu X, Liu H, Hassani D, et al. AaABCG40 Enhances Artemisinin Content and Modulates Drought Tolerance in Artemisia annua [J]. Front Plant Sci, 2020, 11: 950. Huang X, Hou L, Meng J, et al. The Antagonistic Action of Abscisic Acid and Cytokinin Signaling Mediates Drought Stress Response in Arabidopsis [J]. Mol Plant, 2018, 11(7): 970-982. Yazaki K. ABC transporters involved in the transport of plant secondary metabolites [J]. FEBS Lett, 2006, 580(4): 1183-1191. Elejalde-Palmett C, Martinez San Segundo I, Garroum I, et al. ABCG transporters export cutin precursors for the formation of the plant cuticle [J]. Curr Biol, 2021, 31(10): 2111-2123. Gupta A, Rico-Medina A, Caño-Delgado AI. The physiology of plant responses to drought [J]. Science, 2020, 368(6488): 266-269. Kuromori T, Fujita M, Urano K, et al. Overexpression of AtABCG25 enhances the abscisic acid signal in guard cells and improves plant water use efficiency [J]. Plant Sci, 2016, 251: 75-81.)
Objective To clone the SmABCG34 gene from the Salvia miltiorrhiza genome and analyze its bioinformatics and expression characteristics. Methods The SmABCG34 gene was cloned from the S. miltiorrhiza genome, and the Conservative Domain and physicochemical properties of the gene and its encoded protein were analyzed by Bioinformatics Online Website. Quantitative real-time PCR (qRT-PCR) was used to detect the tissue expression specificity of SmABCG34 gene and its response to stress induced by abscisic acid (ABA), trans-zeatin (tZ), and polyethylene glycol-6000 (PEG-6000). Results The CDS of the SmABCG34 gene is 4 347 bp in size and encodes 1 448 amino acids; This protein contains a typical NBD-TMD-NBD-TMD structure and belongs to the full-molecule transporter of the ABCG subfamily; subcellular localization shows that this protein was localizted to the cell membrane. Phylogenetic analysis showed that SmABCG34 was closely related to S. hispanica, with a protein homology of up to 91.23 %. The expression pattern analysis revealed that the SmABCG34 gene was expressed in the roots, stems, leaves and flowers of S. miltiorrhiza with the highest expression in the flowers, followed by the leaves. After treatment with ABA, tZ, and PEG-6000, compared to 0 h, the expression of the SmABCG34 gene was significantly upregulated at 12 h. Conclusion The SmABCG34 gene belongs to the ABCG subfamily and shows a significant response to hormone and stress treatments, providing a scientific basis for further exploration of the function of the SmABCG34 gene.
LIN Yanan, BAI Yanhong, QIU Xinran, ZU Xiuli, YU Juan, LIU Hongyan, GONG Yanan, CHEN Xue, LIU Qian.
Bioinformatics and expression analysis of SmABCG34 gene in Salvia miltiorrhiza[J].
Chinese Traditional and Herbal Drugs,
2026
, 57
(3)
: 1054
-1063
.
DOI: 10.7501/j.issn.0253-2670.2026.03.022
中国药典 [S]. 一部. 2020: 77. Li Z M, Xu S W, Liu P Q. Salvia miltiorrhiza Burge (Danshen): A golden herbal medicine in cardiovascular therapeutics [J]. Acta Pharmacol Sin, 2018, 39(5): 802-824. He G N, Chen G F, Liu W D, et al. Salvianolic acid B: A review of pharmacological effects, safety, combination therapy, new dosage forms, and novel drug delivery routes [J]. Pharmaceutics, 2023, 15(9): 2235. Li Z B, Zou J, Cao D, et al. Pharmacological basis of tanshinone and new insights into tanshinone as a multitarget natural product for multifaceted diseases [J]. Biomed Pharmacother, 2020, 130: 110599. Ma X H, Ma Y, Tang J F, et al. The Biosynthetic Pathways of Tanshinones and Phenolic Acids in Salvia miltiorrhiza [J]. Molecules, 2015, 20(9): 16235-16254. Wu S, Zhu B, Qin L, et al. Transcription Factor: A Powerful Tool to Regulate Biosynthesis of Active Ingredients in Salvia miltiorrhiza [J]. Front Plant Sci, 2021, 12: 622011. Dhara A, Raichaudhuri A. ABCG transporter proteins with beneficial activity on plants [J]. Phytochemistry, 2021, 184: 112663. Kotlyarov S, Kotlyarova A. Biological Functions and Clinical Significance of the ABCG1 Transporter [J]. Biology (Basel), 2024, 14(1): 8. Yan L, Zhang J H, Chen H Y, et al. Genome-wide analysis of ATP-binding cassette transporter provides insight to genes related to bioactive metabolite transportation in Salvia miltiorrhiza [J]. BMC Genomics, 2021, 22(1): 315-315. Huo X H, Pan A, Lei M Y, et al. Genome-wide characterization and functional analysis of ABCG subfamily reveal its role in cutin formation in cotton [J]. Int J Mol Sci, 2023, 24(3): 2379. 孙洁婷, 敬雪皎, 赵丹妮, 等. 植物ABCG转运蛋白功能的研究进展 [J]. 科学通报, 2024, 69(14): 1866-1880. Liu L P, Zhao L H, Chen P J, et al. ATP binding cassette transporters ABCG1 and ABCG16 affect reproductive development via auxin signalling in Arabidopsis [J]. Plant J, 2020, 102(6): 1172-1186. Wang Y Y, Wang Y, Bai H F, et al. An ABCG-type transporter facilitates ABA influx and regulates camptothecin biosynthesis in Camptotheca acuminata [J]. Int J Mol Sci, 2022, 23(24): 16120. Li H, Li Z Y, Yang K B, et al. Comparison analysis of ABCG subfamily in bamboo and the potential function of PeABCG15 in monolignol transport [J]. Plant Physiol Biochem, 2024, 217: 109278. Banasiak J, Borghi L, Stec N, et al. The full-size ABCG transporter of Medicago truncatula is involved in strigolactone secretion, affecting arbuscular mycorrhiza [J]. Front Plant Sci, 2020, 11: 18. Zhao J Z, Yu N N, Ju M, et al. ABC transporter OsABCG18 controls the shootward transport of cytokinins and grain yield in rice [J]. J Exp Bot, 2019, 70(21): 6277-6291. Li Y J, Chen J F, Zhi J Y, et al. The ABC transporter SmABCG1 mediates tanshinones export from the peridermic cells of Salvia miltiorrhiza root [J]. J Integr Plant Biol, 2025, 67(1): 135-149. Schmittgen T D, Livak K J. Analyzing real-time PCR data by the comparative C(T) method [J]. Nat Protoc, 2008, 3(6): 1101-1108. Xiong J, Feng J M, Yuan D X, et al. Tracing the structural evolution of eukaryotic ATP binding cassette transporter superfamily [J]. Sci Rep, 2015, 5: 16724. 王梦龙, 翁灵灵, 吴淑英, 等. ABCG转运蛋白参与植物雄性育性调控的研究进展 [J]. 植物科学学报, 2024, 42(3): 387-394. Crouzet J, Roland J, Peeters E, et al. NtPDR1, a plasma membrane ABC transporter from Nicotiana tabacum, is involved in diterpene transport [J]. Plant Mol Biol, 2013, 82(1/2): 181-192. 何倩. 青蒿转运蛋白基因AaPDR12的克隆与功能分析 [D]. 上海: 上海交通大学, 2018. 李雅静. 丹参根中丹参酮类成分生物合成调控、转运机制及生理活性研究 [D]. 上海: 中国人民解放军海军军医大学, 2022. 李彤. 大豆GmABCG5L基因铁转运的功能研究 [D]. 哈尔滨: 哈尔滨师范大学, 2023. Lee E J, Kim K Y, Zhang J, et al. Arabidopsis seedling establishment under waterlogging requires ABCG5-mediated formation of a dense cuticle layer [J]. New Phytol, 2021, 229(1): 156-172. Jarzyniak K, Banasiak J, Jamruszka T, et al. Early stages of legume-rhizobia symbiosis are controlled by ABCG-mediated transport of active cytokinins [J]. Nat Plants, 2021, 7(4): 428-436. Adebesin F, Widhalm JR, Boachon B, et al. Emission of volatile organic compounds from petunia flowers is facilitated by an ABC transporter [J]. Science, 2017, 356(6345): 1386-1388. 董润鑫, 徐芯, 李彦妮, 等. 丹参花总酚酸和花青素的含量测定 [J]. 四川农业科技, 2024, 43(9): 70-75. Reshi ZA, Ahmad W, Lukatkin AS, et al. From Nature to Lab: A Review of Secondary Metabolite Biosynthetic Pathways, Environmental Influences, and In Vitro Approaches [J]. Metabolites, 2023, 13(8): 895. Gai Z, Wang Y, Ding Y, et al. Exogenous abscisic acid induces the lipid and flavonoid metabolism of tea plants under drought stress [J]. Sci Rep, 2020, 10(1): 12275. Fu X, Liu H, Hassani D, et al. AaABCG40 Enhances Artemisinin Content and Modulates Drought Tolerance in Artemisia annua [J]. Front Plant Sci, 2020, 11: 950. Huang X, Hou L, Meng J, et al. The Antagonistic Action of Abscisic Acid and Cytokinin Signaling Mediates Drought Stress Response in Arabidopsis [J]. Mol Plant, 2018, 11(7): 970-982. Yazaki K. ABC transporters involved in the transport of plant secondary metabolites [J]. FEBS Lett, 2006, 580(4): 1183-1191. Elejalde-Palmett C, Martinez San Segundo I, Garroum I, et al. ABCG transporters export cutin precursors for the formation of the plant cuticle [J]. Curr Biol, 2021, 31(10): 2111-2123. Gupta A, Rico-Medina A, Caño-Delgado AI. The physiology of plant responses to drought [J]. Science, 2020, 368(6488): 266-269. Kuromori T, Fujita M, Urano K, et al. Overexpression of AtABCG25 enhances the abscisic acid signal in guard cells and improves plant water use efficiency [J]. Plant Sci, 2016, 251: 75-81.
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