中草药

铁皮石斛花外泌体样纳米颗粒的制备及体外抗肿瘤作用

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中草药 | 药剂与工艺 2026,57(19): 7573-7586

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中草药 |药剂与工艺 2026 , 57 (19) : 7573 -7586

铁皮石斛花外泌体样纳米颗粒的制备及体外抗肿瘤作用

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肖吕浪^1,2,3, 王江英¹, 张芳瑕^1,2,3, 余梦竹^1,2,3, 周英^1,2, 朱月^1,2,3, 汪祖华^1,2,3

作者信息

通讯作者:

汪祖华

作者简介:

肖吕浪: 肖吕浪，男，硕士研究生，研究方向为纳米创新药物制剂开发。E-mail:17671769374@163.com

Preparation and in vitro anti-tumor effects of extracellular vesicle-like nanoparticles from Dendrobium officinale flowers

XIAO Lyulang^1,2,3, WANG Jiangying¹, ZHANG Fangxia^1,2,3, YU Mengzhu^1,2,3, ZHOU Ying^1,2, ZHU Yue^1,2,3, WANG Zuhua^1,2,3

Affiliations

doi: 10.7501j.issn.0253-2670.2026.19.008

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摘要

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目的从铁皮石斛Dendrobium officinale花中分离制备细胞外泌体样纳米颗粒（extracellular vesicle-like nanoparticles from D. officinale flowers，DF-EVLPs），并评估其对结肠癌细胞的特异性靶向能力，同时考察其对结肠癌细胞的体外靶向与抗肿瘤作用，为开发基于药食同源植物的靶向递送系统提供实验依据。方法从铁皮石斛花中分离提取DF-EVLPs，并对其形态和粒径进行表征。利用pKH26荧光标记观察DF-EVLPs被小鼠结肠癌CT26细胞的摄取情况；通过CCK-8法、划痕愈合实验和流式细胞术及Western blotting实验分别评估DF-EVLPs对CT26细胞增殖、迁移及凋亡的影响及其机制作用。结果所获得的DF-EVLPs呈均一的杯状形态，平均粒径为（156.9±7.2）nm。细胞摄取实验显示，DF-EVLPs能够被CT26细胞高效内化，且对CT26细胞的摄取效率显著高于正常结肠上皮细胞，表明其具有良好的特异性靶向能力。体外功能实验显示，DF-EVLPs以浓度相关方式抑制CT26细胞增殖，显著降低细胞迁移能力，并诱导细胞凋亡。结论成功制备了铁皮石斛花来源的外泌体样纳米颗粒，DF-EVLPs作为一种新型食品来源纳米治疗剂，能够靶向结肠癌细胞并发挥体外抗肿瘤活性。揭示了铁皮石斛花的一种新生物活性功能，为基于“药食同源”理念的结直肠癌膳食干预提供了实验依据。

关键词

铁皮石斛花 / 外泌体样纳米颗粒 / 结肠癌 / 抗肿瘤作用 / 靶向递送系统

Abstract

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Objective To isolate and prepare extracellular vesicle-like nanoparticles from Tiepishihu (Dendrobium officinale) flowers (DF-EVLPs), and evaluate their specific targeting ability for colon cancer cells, as well as investigate their in vitro targeting and anti-tumor effects against colon cancer cells, in order to provide experimental evidence for the development of targeted delivery systems based on medicinal and edible plants. Methods DF-EVLPs were isolated from D. officinale flowers and their morphology and particle size were characterized. The uptake of DF-EVLPs by CT26 colon cancer cells was observed using pKH26 fluorescence labeling. The effects of DF-EVLPs on the proliferation, migration and apoptosis of CT26 cells and their mechanism were evaluated using CCK-8 assay, scratch wound healing experiment, flow cytometry and Western blotting. Results The obtained DF-EVLPs showed a uniform cup-shaped morphology with an average particle size of (156.9 ± 7.2) nm. The cell uptake experiment demonstrated that DF-EVLPs could be efficiently internalized by CT26 colon cancer cells, and the uptake efficiency of DF-EVLPs for colon cancer cells was significantly higher than that of normal colonic epithelial cells, indicating their good specific targeting ability. The in vitro functional experiments showed that DF-EVLPs inhibited the proliferation of CT26 cells in a concentration-dependent manner, significantly reduced cell migration ability, and induced cell apoptosis. Conclusion This study successfully prepared extracellular vesicle-like nanoparticles from D. officinale flowers. As a new type of food-derived nanotherapeutic agent, DF-EVLPs can specifically target colon cancer cells and exert in vitro anti-tumor activity. This study reveals a new biological activity function of D. officinale flowers and provides experimental evidence for dietary intervention for colorectal cancer based on the “medicinal and edible” concept.

Key words

Dendrobium officinale flower / extracellular vesicle-like nanoparticles / colon cancer / anti-tumor effect / targeted delivery system

引用本文

肖吕浪, 王江英, 张芳瑕, 余梦竹, 周英, 朱月, 汪祖华. 铁皮石斛花外泌体样纳米颗粒的制备及体外抗肿瘤作用. 中草药, 2026 , 57 (19) : 7573 -7586 . DOI: 10.7501j.issn.0253-2670.2026.19.008

XIAO Lyulang, WANG Jiangying, ZHANG Fangxia, YU Mengzhu, ZHOU Ying, ZHU Yue, WANG Zuhua. Preparation and in vitro anti-tumor effects of extracellular vesicle-like nanoparticles from Dendrobium officinale flowers[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (19) : 7573 -7586 . DOI: 10.7501j.issn.0253-2670.2026.19.008

基金

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贵州省现代中药创制全省重点实验室 (黔科合平台［2025］019); 中药制剂与大健康产品开发贵州省科技创新领军人才工作站 (黔科合平台KXJZ［2025］042); 贵州中医药大学纳米药物技术研究中心 (贵中医ZX合字［2024］070)

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2026年第57卷第19期

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doi: 10.7501j.issn.0253-2670.2026.19.008

接收时间：2026-04-21
首发时间：2026-10-29

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收稿日期：2026-04-21

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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