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鸡血藤查耳酮合成酶基因SsCHS1克隆及功能分析

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中草药 | 药材与资源 2026,57(18): 7397-7406

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中草药 |药材与资源 2026 , 57 (18) : 7397 -7406

鸡血藤查耳酮合成酶基因SsCHS1克隆及功能分析

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谢月英¹, 姚李祥¹, 柯芳¹, 林泉¹, 余丽莹¹, 秦双双¹

作者信息

通讯作者:

余丽莹

作者简介:

谢月英: 谢月英（1978—），女，副研究员，研究方向为中药资源。E-mail:372020561@qq.com

Cloning and functional characterization of chalcone synthase gene SsCHS1 from Spatholobus suberectus

XIE Yueying¹, YAO Lixiang¹, KE Fang¹, LIN Quan¹, YU Liying¹, QIN Shuangshuang¹

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doi: 10.7501/j.issn.0253-2670.2026.18.026

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摘要

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目的克隆鸡血藤Spatholobus suberectus查耳酮合成酶基因（chalcone synthase，CHS）SsCHS1，开展生物信息学及基因功能研究，以期解析其在黄酮生物合成中的作用。方法以鸡血藤cDNA为模板，借助RT-PCR技术克隆SsCHS1，对该基因的序列特征、蛋白结构与功能等开展系统的生物信息学解析。构建SsCHS1过表达载体，遗传转化本氏烟草，测定转基因植株中总黄酮含量及黄酮合成途径中关键酶基因的表达水平。结果 SsCHS1基因全长1 176 bp，其编码蛋白由391个氨基酸组成，理论相对分子质量为42 825.35，等电点为6.10，属于亲水性蛋白；与豆科植物甘草、大豆等物种的CHS蛋白具有较高同源性。启动子分析表明，SsCHS1富集了激素响应、光响应、胁迫响应及转录因子结合位点相关的多种顺式作用元件。功能分析显示，过表达SsCHS1可显著促进转基因烟草中黄酮的积累，并上调黄酮生物合成途径中关键酶基因的表达水平。结论鸡血藤SsCHS1基因能够促进黄酮的生物合成，该基因的发掘为深入解析鸡血藤活性成分的合成机制提供了重要的理论依据与候选基因。

关键词

鸡血藤 / SsCHS1 / 基因克隆 / 黄酮 / 基因功能

Abstract

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Objective To clone the chalcone synthase gene SsCHS1 from Spatholobus suberectus and conduct bioinformatics and functional analyses, in order to elucidate its role in flavonoid biosynthesis. Methods Using cDNA from S. suberectus as a template, SsCHS1 was cloned by RT-PCR. Systematic bioinformatics analyses were performed on the sequence characteristics, protein structure, and function of the gene. An SsCHS1 overexpression vector was constructed and genetically transformed into Nicotiana benthamiana. The total flavonoid content and the expression levels of key enzyme genes in the flavonoid biosynthesis pathway in transgenic plants were determined. Results The full-length SsCHS1 gene was 1 176 bp, encoding a protein consisting of 391 amino acids, with a theoretical relative molecular mass of 42 825.35, an isoelectric point of 6.10, and was characterized as a hydrophilic protein. It showed high homology with CHS proteins from leguminous plants such as Glycyrrhiza uralensis and Glycine max. Promoter analysis revealed that SsCHS1 contains multiple cis-acting elements associated with hormone response, light response, stress response, and transcription factor binding sites. Functional analysis demonstrated that overexpression of SsCHS1 significantly promoted flavonoid accumulation in transgenic tobacco and upregulated the expression levels of key enzyme genes in the flavonoid biosynthesis pathway. Conclusion The SsCHS1 gene from S. suberectus promotes flavonoid biosynthesis. The identification of this gene provides an important theoretical basis and a candidate gene for further elucidation of the biosynthetic mechanisms of active components in S. suberectus.

Key words

Spatholobus suberectus Dunn / SsCHS1 / gene cloning / flavonoid / gene function

引用本文

谢月英, 姚李祥, 柯芳, 林泉, 余丽莹, 秦双双. 鸡血藤查耳酮合成酶基因SsCHS1克隆及功能分析. 中草药, 2026 , 57 (18) : 7397 -7406 . DOI: 10.7501/j.issn.0253-2670.2026.18.026

XIE Yueying, YAO Lixiang, KE Fang, LIN Quan, YU Liying, QIN Shuangshuang. Cloning and functional characterization of chalcone synthase gene SsCHS1 from Spatholobus suberectus[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (18) : 7397 -7406 . DOI: 10.7501/j.issn.0253-2670.2026.18.026

基金

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国家重点研发计划项目 (2024YFC3506703); 广西中医药适宜技术开发与推广项目 (GZSY2025005); 广西自然科学基金项目 (2025GXNSFAA069354)

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中国药典[S]. 一部. 2025:209-210.
秦双双,朱艳霞,韦坤华,等.鸡血藤的本草沿革与黄酮类成分及其药理学研究进展[J]. 中国中药杂志,2018, 43(11):2216-2223.
Chen H L, Yang J, Fu Y F, et al. Effect of total flavonoids of Spatholobus suberectus Dunn on PCV2 induced oxidative stress in RAW264.7 cells[J]. BMC Complement Altern Med, 2017, 17(1):244.
Pan Y H, Luo X M, Gong P Y. Spatholobi caulis:A systematic review of its traditional uses, chemical constituents, biological activities and clinical applications[J]. J Ethnopharmacol, 2023, 317:116854.
Liu X Y, Zhang Y B, Yang X W, et al. Anti-inflammatory activity of some characteristic constituents from the vine stems of Spatholobus suberectus[J]. Molecules, 2019,24(20):3750.
Chen S R, Wang A Q, Lin L G, et al. In vitro study on antihepatitis C virus activity of Spatholobus suberectus Dunn[J]. Molecules, 2016, 21(10):1367.
Peng F, Meng C W, Zhou Q M, et al. Cytotoxic evaluation against breast cancer cells of isoliquiritigenin analogues from Spatholobus suberectus and their synthetic derivatives[J]. J Nat Prod, 2016, 79(1):248-251.
Sunil L, Shetty N P. Biosynthesis and regulation of anthocyanin pathway genes[J]. Appl Microbiol Biotechnol, 2022, 106(5/6):1783-1798.
覃炜棋,林泉,梁莹,等.鸡血藤不同器官转录组分析与儿茶素生物合成基因研究[J]. 中国中药杂志, 2025,50(12):3297-3306.
Liu H M, Liu Z J, Wu Y, et al. Regulatory mechanisms of anthocyanin biosynthesis in apple and pear[J]. Int J Mol Sci, 2021, 22(16):8441.
Wang W K, Schaal B A, Chiou Y M, et al. Diverse selective modes among orthologs/paralogs of the Chalcone synthase(Chs)gene family of Arabidopsis thaliana and its relative A. halleri ssp. gemmifera[J]. Mol Phylogenet Evol, 2007, 44(2):503-520.
Wang L X, Lam P Y, Lui A C W, et al. Flavonoids are indispensable for complete male fertility in rice[J]. J Exp Bot, 2020, 71(16):4715-4728.
Yang M, Yin M Z, Chu S S, et al. Colour, chemical compounds, and antioxidant capacity of Astragali Radix based on untargeted metabolomics and targeted quantification[J]. Phytochem Anal, 2022, 33(4):599-611.
Guo D D, Gao Y, Liu F, et al. Integrating molecular characterization and metabolites profile revealed CtCHI1’s significant role in Carthamus tinctorius L.[J]. BMC Plant Biol, 2019, 19(1):376.
Helariutta Y, Elomaa P, Kotilainen M, et al. Chalcone synthase-like genes active during Corolla development are differentially expressed and encode enzymes with different catalytic properties in Gerbera hybrida(Asteraceae)[J]. Plant Mol Biol, 1995, 28(1):47-60.
敖志杰,张惊宇,曾娟,等.灰毡毛忍冬CHS1基因克隆及功能分析[J]. 中草药, 2025, 56(17):6334-6345.
Qin S S, Wu L Q, Wei K H, et al. A draft genome for Spatholobus suberectus[J]. Sci Data, 2019, 6(1):113.
Qin S S, Wei G L, Lin Q, et al. Analysis of the Spatholobus suberectus full-length transcriptome identified an R2R3-MYB transcription factor-encoding gene SsMYB158 that regulates flavonoid biosynthesis[J]. Plant Physiol Biochem, 2024, 214:108929.
Han Y H, Ding T, Su B, et al. Genome-wide identification,characterization and expression analysis of the Chalcone synthase family in maize[J]. Int J Mol Sci, 2016, 17(2):161.
Anguraj Vadivel A K, Krysiak K, Tian G, et al. Genomewide identification and localization of Chalcone synthase family in soybean(Glycine max L. Merr)[J]. BMC Plant Biol, 2018, 18(1):325.
Harshavardhan Koduri P K, Gordon G S, Barker E I, et al.Genome-wide analysis of the Chalcone synthase superfamily genes of Physcomitrella patens[J]. Plant Mol Biol, 2010, 72(3):247-263.
Winkel-Shirley B. Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology[J]. Plant Physiol, 2001, 126(2):485-493.
Dao T T H, Linthorst H J M, Verpoorte R. Chalcone synthase and its functions in plant resistance[J]. Phytochem Rev, 2011, 10(3):397-412.
Saito K, Yonekura-Sakakibara K, Nakabayashi R, et al.The flavonoid biosynthetic pathway in Arabidopsis:Structural and genetic diversity[J]. Plant Physiol Biochem, 2013, 72:21-34.
Ferrer J L, Jez J M, Bowman M E, et al. Structure of Chalcone synthase and the molecular basis of plant polyketide biosynthesis[J]. Nat Struct Biol, 1999, 6(8):775-784.
李鹏飞,王爱凡,黄佳慧,等.薰衣草花青素合成相关基因La CHS的克隆与功能分析[J]. 浙江农林大学学报, 2025, 42(5):1048-1058.
单丽伟,汪勇,王美玲,等.大豆类黄酮生物合成关键酶CHS基因的克隆及表达分析[J]. 西北植物学报,2012, 32(11):2164-2168.
Jenkins G I, Long J C, Wade H K, et al. UV and blue light signalling:Pathways regulating Chalcone synthase gene expression in Arabidopsis[J]. New Phytol, 2001, 151(1):121-131.
Hu H F, Shi B, Zhu W C, et al. Genome-wide identification,characterization and expression analysis of mango(Mangifera indica L.)Chalcone synthase(CHS)genes in response to light[J]. Horticulturae, 2022, 8(10):968.
Jiang T, Guo K Y, Liu L D, et al. Integrated transcriptomic and metabolomic data reveal the flavonoid biosynthesis metabolic pathway in Perilla frutescens(L.)leaves[J]. Sci Rep, 2020, 10:16207.

2026年第57卷第18期

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doi: 10.7501/j.issn.0253-2670.2026.18.026

接收时间：2026-05-27
首发时间：2026-10-29

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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