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基于脾脏转录组学探究驴胶补血颗粒改善大鼠产后气血双虚症状的作用机制

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中草药 | 药理与临床 2026,57(17): 6848-6858

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中草药 |药理与临床 2026 , 57 (17) : 6848 -6858

基于脾脏转录组学探究驴胶补血颗粒改善大鼠产后气血双虚症状的作用机制

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程帆¹, 陈晓翌¹, 颜冬兰², 黄胜², 张宏宇¹, 田俊生^1,3

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田俊生

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程帆: 程帆，女，硕士研究生，研究方向为中药药理作用机制研究。E-mail:17803554115@139.com

Mechanism of Lvjiao Buxue Granules in improving postpartum qi and blood deficiency symptoms in rats based on spleen transcriptomics

CHENG Fan¹, CHEN Xiaoyi¹, YAN Donglan², HUANG Sheng², ZHANG Hongyu¹, TIAN Junsheng^1,3

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doi: 10.7501/j.issn.0253-2670.2026.17.016

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目的基于脾脏转录组学探究驴胶补血颗粒改善产后气血双虚模型大鼠的作用机制。方法产后24 h的大鼠采用连续2周负重游泳联合眼眶放血建立产后气血双虚模型，造模成功后随机分为模型组及驴胶补血颗粒低、中、高剂量（0.84、1.68、3.36 g/kg）组和当归补血丸（1.89 g/kg）组，另取产后24 h的大鼠作为对照组。给药干预2周后，通过气虚评分、血虚评分、血常规指标、免疫器官指数、免疫炎症指标等综合评价驴胶补血颗粒对模型大鼠产后气血双虚症状的改善作用。通过转录组学分析探究驴胶补血颗粒干预后对大鼠脾脏差异表达基因及相关信号通路的影响，采用Western blotting和qRT-PCR对关键信号通路中的蛋白和基因表达进行验证。结果驴胶补血颗粒可以显著改善产后气血双虚模型大鼠的气虚评分、血虚评分、血常规指标（P＜0.05、0.01），降低血清中炎症相关指标白细胞介素-6（interleukin-6，IL-6）、IL-1β、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）水平（P＜0.05、0.01），升高免疫调节相关指标免疫球蛋白A（immunoglobulin A，IgA）、IgG、IgM水平（P＜0.05、0.01），抑制脾脏组织中丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）通路相关蛋白和基因的表达（P＜0.05、0.01、0.001）。结论驴胶补血颗粒通过抑制MAPK通路的磷酸化，调节机体免疫功能，降低炎性反应发生，从而改善大鼠产后气血双虚症状。

关键词

驴胶补血颗粒 / 产后气血双虚 / 转录组学 / MAPK信号通路 / 免疫功能 / 炎症反应

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Objective To explore the mechanism of Lvjiao Buxue Granules (驴胶补血颗粒) in improving postpartum qi and blood deficiency model rats based on spleen transcriptomics. Methods Rats 24 h postpartum were subjected to continuous two weeks weight-bearing swimming combined with orbital bloodletting to establish a postpartum qi and blood deficiency model. After successful modeling, they were randomly divided into model group, Lvjiao Buxue Granules low-, medium-, high-dose (0.84, 1.68, 3.36 g/kg) groups and Danggui Buxue Pills (当归补血丸, 1.89 g/kg) group. Rats 24 h postpartum were also selected as the control group. After two weeks of drug intervention, the comprehensive evaluation of the improvement effect of Lvjiao Buxue Granules on postpartum qi and blood deficiency symptoms in model rats was conducted through qi deficiency score, blood deficiency score, blood routine index, immune organ index, immune inflammation index and other indicators. Through transcriptome analysis, the effects of Lvjiao Buxue Granules intervention on differentially expressed genes and related signaling pathways in spleen of rats were explored. Western blotting and qRT-PCR were used to verify the protein and gene expressions in key signaling pathways. Results Lvjiao Buxue Granules could significantly improve qi deficiency score, blood deficiency score and blood routine indicators of postpartum qi and blood deficiency model rats (P < 0.05, 0.01), reduce inflammation related indicators such as interleukin-6 (IL-6), IL-1β and tumor necrosis factor-α (TNF-α) levels in serum (P < 0.05, 0.01), increase immune regulation related indicators such as immunoglobulin A (IgA), IgG and IgM levels (P < 0.05, 0.01), and inhibit the expressions of mitogen-activated protein kinase (MAPK) pathway related proteins and genes in spleen tissue (P < 0.05, 0.01, 0.001). Conclusion Lvjiao Buxue Granules can improve the symptoms of postpartum qi and blood deficiency in rats by inhibiting the phosphorylation of MAPK pathway, regulating the immune function of body and reducing the occurrence of inflammatory reactions.

Key words

Lvjiao Buxue Granules / postpartum qi and blood deficiency / transcriptomics / MAPK signal pathway / immune function / inflammatory response

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程帆, 陈晓翌, 颜冬兰, 黄胜, 张宏宇, 田俊生. 基于脾脏转录组学探究驴胶补血颗粒改善大鼠产后气血双虚症状的作用机制. 中草药, 2026 , 57 (17) : 6848 -6858 . DOI: 10.7501/j.issn.0253-2670.2026.17.016

CHENG Fan, CHEN Xiaoyi, YAN Donglan, HUANG Sheng, ZHANG Hongyu, TIAN Junsheng. Mechanism of Lvjiao Buxue Granules in improving postpartum qi and blood deficiency symptoms in rats based on spleen transcriptomics[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (17) : 6848 -6858 . DOI: 10.7501/j.issn.0253-2670.2026.17.016

基金

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国家自然科学基金面上项目 (82374153); 企业委托横向科研项目 (02260123030013)

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温海莹. 产后多虚多瘀动物模型的实验研究[D]. 成都: 成都中医药大学, 2008.
陈晓翌, 程帆, 颜冬兰, 等. 乳汁代谢组学结合生物靶标网络分析探究驴胶补血颗粒改善大鼠产后气血双虚的作用机制[J]. 药学学报, 2025, 60(11): 3435-3446.
陈晓翌. 驴胶补血颗粒改善大鼠产后气血双虚的药效与作用机制研究[D]. 太原: 山西大学, 2025.
Szpunar M J, Malaktaris A, Baca S A, et al. Are alterations in estradiol, cortisol, and inflammatory cytokines associated with depression during pregnancy and postpartum? An exploratory study [J]. Brain Behav Immun Health, 2021, 16: 100309.
廖安飞, 许璇, 白杨, 等. 不同分娩方式产后凝血功能的变化及与炎症应激反应指标的相关性[J]. 基层医学论坛, 2025, 29(5): 21-24.
Chang W C, Yen C C, Cheng C P, et al. Chinese herbal decoction (Danggui Buxue Tang) supplementation augments physical performance and facilitates physiological adaptations in swimming rats [J]. Pharm Biol, 2020, 58(1): 545-552.
Chen J P, Tsim K W K. A review of edible jujube, the Ziziphus jujuba fruit: A heath food supplement for anemia prevalence [J]. Front Pharmacol, 2020, 11: 593655.
薛红莉. 八珍汤补益气血作用研究进展[J]. 河南中医, 2010, 30(10): 1039-1041.
Lu S, Sun X B, Zhou Z B, et al. Mechanism of Bazhen Decoction in the treatment of colorectal cancer based on network pharmacology, molecular docking, and experimental validation [J]. Front Immunol, 2023, 14: 1235575.
费国娟, 杨芙蓉, 汪鸣, 等. 加味甘麦大枣汤对气血两虚型产后抑郁症患者肠道菌群和血清5-HT的影响[J]. 中国微生态学杂志, 2024, 36(2): 211-217.
You Y, Luo L, You Y Y, et al. Shengmai Yin Formula modulates the gut microbiota of spleen-deficiency rats [J]. Chin Med, 2020, 15: 114.
Fang Y W, Chen S F, Wang M L, et al. Effects of traditional Chinese medicine-assisted intervention on improving postpartum lactation: A systematic review and Meta-analysis [J]. Heliyon, 2024, 10(6): e27154.
Bao Q N, Yin Z H, Zhou Y F, et al. Efficacy and safety of acupuncture for postpartum hypogalactia: A systematic review and Meta-analysis of randomized controlled trials [J]. PLoS One, 2024, 19(6): e0303948.
邵渝, 刘细寒, 邱菊华, 等. 调气固本推拿法干预气血虚弱型产后缺乳的临床研究[J]. 中医药导报, 2015, 21(23): 94-96.
中国药典[S]. 一部. 2025: 1022.
Rao S, Ghosh D, Asturias E J, et al. What can we learn about influenza infection and vaccination from transcriptomics? [J]. Hum Vaccines Immunother, 2019, 15(11): 2615-2623.
马玉朋, 只德广, 边疆, 等. 补血益母丸对药物流产大鼠模型与气血双虚大鼠模型的药效作用研究[J]. 云南中医中药杂志, 2025, 46(10): 79-86.
李亚梅, 宾雨飞, 夏伯候, 等. 补血益母丸对气血双虚模型小鼠的药效作用研究[J]. 中药药理与临床, 2020, 36(3): 158-164.
林锦涛, 李宇卫. 芍药甘草汤药理作用机制及临床应用研究新进展[J]. 内蒙古中医药, 2026, 45(2): 163-165.
曾荣华, 周露, 欧阳厚淦, 等. 针刺“足三里”穴对脾虚证模型大鼠肠系膜淋巴结T淋巴细胞亚群的影响[J]. 中国组织工程研究, 2018, 22(4): 576-581.
Dias J A, Guimarães I C, Cantuária V L, et al. Maternal obesity modulates postpartum inflammatory and hormonal profiles, without detectable differences in tested redox markers [J]. Am J Reprod Immunol, 2026, 95(3): e70227.
王兴昌, 史发林, 李玲玉, 等. TNF-α、IL-2、IgM、IgG和IgA在重离子治疗肿瘤患者中的变化及其临床意义[J]. 分子诊断与治疗杂志, 2026, 18(2): 394-396.
Wan M C, Wang Y L, Zeng L, et al. Serum immune indicators including IgM, IgA, and IgG levels and quality of life of patients with gastrointestinal tumours during chemotherapy [J]. J Med Biochem, 2025, 44(4): 814-821.
Zafar A, Khalid M, Alsaidan O A, et al. Protective effect of erucic acid against cyclophosphamide-induced immunosuppression via IFN-γ/TNF-α/IgG pathways: In vivo network pharmacology-based analysis, and molecular docking [J]. Food Funct, 2026, 17(2): 716-733.
Abdukhakimova D, Dossybayeva K, Almukhamedova Z, et al. Serum immunoglobulin A (IgA) levels in children affected with juvenile idiopathic arthritis [J]. Heliyon, 2023, 9(7): e17479.
Jin F Q, Zhang Q, Weng J P, et al. Serum IgA, IgG, IgM levels, total protein (TP), albumin (ALB) changes in elderly hip fracture patients after surgery and nutritional support [J]. J Med Biochem, 2025, 44(4): 822-830.
刘杰, 胡粤杭, 应汝炯, 等. 柴胡桂枝汤治疗肝郁脾虚型慢性疲劳综合征的临床疗效及对患者免疫功能的影响[J]. 时珍国医国药, 2019, 30(6): 1414-1416.
Hammad A M, Ibrahim Y A, Khdair S I, et al. Metformin reduces oxandrolone- induced depression-like behavior in rats via modulating the expression of IL-1β, IL-6, IL-10 and TNF-α [J]. Behav Brain Res, 2021, 414: 113475.
张鹤, 白宇宁, 刘绍能, 等. 现代医学视角下中医“脾主运化”探析[J]. 北京中医药, 2022, 41(2): 179-181.
Khan N, Kaur S, Knuth C M, et al. CNS-spleen axis-a close interplay in mediating inflammatory responses in burn patients and a key to novel burn therapeutics [J]. Front Immunol, 2021, 12: 720221.
Arafa E A, Refaey M S, Abd El-Ghafar O A M, et al. The promising therapeutic potentials of ginsenosides mediated through p38 MAPK signaling inhibition [J]. Heliyon, 2021, 7(11): e08354.
Ma X J, Yan W H, He N. Lidocaine attenuates hypoxia/reoxygenation-induced inflammation, apoptosis and ferroptosis in lung epithelial cells by regulating the p38 MAPK pathway [J]. Mol Med Rep, 2022, 25(5): 150.
Lee S, Rauch J, Kolch W. Targeting MAPK signaling in cancer: Mechanisms of drug resistance and sensitivity [J]. Int J Mol Sci, 2020, 21(3): 1102.
谢育娣, 洪艺煌, 曾素芬, 等. 产前丝裂原活化蛋白激酶相关因子表达与二次剖宫产产后出血的相关性及氨甲环酸的治疗效果分析[J]. 中国妇幼保健, 2025, 40(17): 3122-3125.
Zhao Y L, Su M Y, Cheng Y, et al. Taohong Siwu Decoction mitigates abnormal uterine bleeding by suppressing ferroptosis in endometrial epithelial cell through p38 MAPK signaling pathway [J]. J Ethnopharmacol, 2026, 365: 121532.

2026年第57卷第17期

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doi: 10.7501/j.issn.0253-2670.2026.17.016

接收时间：2026-04-27
首发时间：2026-10-29

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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