中草药

基于Illumina高通量测序的蛇莓不同组织内生细菌多样性分析与功能预测

PDF下载

张慧 ¹ , 李贵文 ¹,² , 杨胜利 ² , 朱李娜 ¹ , 王靖宇 ¹ , 肖功年 ¹

中草药 | 药材与资源 2026,57(16): 6510-6520

收起

中草药 |药材与资源 2026 , 57 (16) : 6510 -6520

基于Illumina高通量测序的蛇莓不同组织内生细菌多样性分析与功能预测

全屏

[Author(id=1319981779973420024, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=张慧, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=2, ext={CN=AuthorExt(id=1319981780040528890, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, authorId=1319981779973420024, language=CN, stringName=张慧, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1.浙江科技大学生物与化学工程学院, 浙江杭州 310023浙江科技大学生物与化学工程学院, 浙江杭州 310023, bio=张慧: 张慧(1972—),女,教授级高级工程师,硕士生导师,主要从事功能食品研究。E-mail:zhanghui0461@zust.edu.cn, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1319981779822425076, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, xref=1, ext=[AuthorCompanyExt(id=1319981779830813685, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, companyId=1319981779822425076, language=CN, country=null, province=null, city=null, postcode=null, companyName=浙江科技大学生物与化学工程学院, 浙江杭州 310023, departmentName=null, remark=^1.浙江科技大学生物与化学工程学院, 浙江杭州 310023)])]), Author(id=1319981780116026363, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=李贵文, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=2, ext={CN=AuthorExt(id=1319981780204106750, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, authorId=1319981780116026363, language=CN, stringName=李贵文, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹,², address=^1.浙江科技大学生物与化学工程学院, 浙江杭州 310023,^2.浙江工业大学药学院, 浙江杭州 310014浙江科技大学生物与化学工程学院, 浙江杭州 310023, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1319981779822425076, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, xref=1, ext=[AuthorCompanyExt(id=1319981779830813685, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, companyId=1319981779822425076, language=CN, country=null, province=null, city=null, postcode=null, companyName=浙江科技大学生物与化学工程学院, 浙江杭州 310023, departmentName=null, remark=^1.浙江科技大学生物与化学工程学院, 浙江杭州 310023)]), AuthorCompany(id=1319981779893728246, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, xref=2, ext=[AuthorCompanyExt(id=1319981779902116855, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, companyId=1319981779893728246, language=CN, country=null, province=null, city=null, postcode=null, companyName=浙江工业大学药学院, 浙江杭州 310014, departmentName=null, remark=^2.浙江工业大学药学院, 浙江杭州 310014)])]), Author(id=1319981780271215615, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=杨胜利, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=2, ext={CN=AuthorExt(id=1319981780342517760, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, authorId=1319981780271215615, language=CN, stringName=杨胜利, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=², address=^2.浙江工业大学药学院, 浙江杭州 310014浙江工业大学药学院, 浙江杭州 310014, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1319981779893728246, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, xref=2, ext=[AuthorCompanyExt(id=1319981779902116855, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, companyId=1319981779893728246, language=CN, country=null, province=null, city=null, postcode=null, companyName=浙江工业大学药学院, 浙江杭州 310014, departmentName=null, remark=^2.浙江工业大学药学院, 浙江杭州 310014)])]), Author(id=1319981780506095617, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=朱李娜, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=2, ext={CN=AuthorExt(id=1319981780912943107, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, authorId=1319981780506095617, language=CN, stringName=朱李娜, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1.浙江科技大学生物与化学工程学院, 浙江杭州 310023浙江科技大学生物与化学工程学院, 浙江杭州 310023, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1319981779822425076, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, xref=1, ext=[AuthorCompanyExt(id=1319981779830813685, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, companyId=1319981779822425076, language=CN, country=null, province=null, city=null, postcode=null, companyName=浙江科技大学生物与化学工程学院, 浙江杭州 310023, departmentName=null, remark=^1.浙江科技大学生物与化学工程学院, 浙江杭州 310023)])]), Author(id=1319981781001023492, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, orderNo=5, firstName=null, middleName=null, lastName=null, nameCn=王靖宇, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=2, ext={CN=AuthorExt(id=1319981781323984902, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, authorId=1319981781001023492, language=CN, stringName=王靖宇, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1.浙江科技大学生物与化学工程学院, 浙江杭州 310023浙江科技大学生物与化学工程学院, 浙江杭州 310023, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1319981779822425076, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, xref=1, ext=[AuthorCompanyExt(id=1319981779830813685, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, companyId=1319981779822425076, language=CN, country=null, province=null, city=null, postcode=null, companyName=浙江科技大学生物与化学工程学院, 浙江杭州 310023, departmentName=null, remark=^1.浙江科技大学生物与化学工程学院, 浙江杭州 310023)])]), Author(id=1319981781386899463, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, orderNo=6, firstName=null, middleName=null, lastName=null, nameCn=肖功年, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=2, ext={CN=AuthorExt(id=1319981783060426762, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, authorId=1319981781386899463, language=CN, stringName=肖功年, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1.浙江科技大学生物与化学工程学院, 浙江杭州 310023浙江科技大学生物与化学工程学院, 浙江杭州 310023, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1319981779822425076, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, xref=1, ext=[AuthorCompanyExt(id=1319981779830813685, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1319981777058378737, companyId=1319981779822425076, language=CN, country=null, province=null, city=null, postcode=null, companyName=浙江科技大学生物与化学工程学院, 浙江杭州 310023, departmentName=null, remark=^1.浙江科技大学生物与化学工程学院, 浙江杭州 310023)])])]

张慧¹, 李贵文¹,², 杨胜利², 朱李娜¹, 王靖宇¹, 肖功年¹

作者信息

^1.浙江科技大学生物与化学工程学院, 浙江杭州 310023

^2.浙江工业大学药学院, 浙江杭州 310014

作者简介:

张慧: 张慧(1972—),女,教授级高级工程师,硕士生导师,主要从事功能食品研究。E-mail:zhanghui0461@zust.edu.cn

Diversity analysis and functional prediction of endophytic bacteria in different tissues of Duchesnea indica based on Illumina high-throughput sequencing

Affiliations

出版时间: 2026-08-28 doi: 10.7501/j.issn.0253-2670.2026.16.023

文章导航

摘要

收起

目的剖析蛇莓Duchesnea indica不同组织内生细菌群落的多样性及其功能，揭示内生细菌与蛇莓药用活性间的潜在联系。方法以蛇莓组织根、茎、叶、果中内生细菌为研究对象，运用Illumina高通量技术对群落DNA片段进行双端测序，借助DADA2和Vsearch方法处理序列，进行生物信息学分析及功能潜能预测。结果蛇莓内生细菌基因组经高通量测序，共获取1 914 360条原始序列，在“门”水平上，以变形菌门（Proteobacteria）为优势菌群。不同组织中内生细菌以茎内丰富度最高，根内多样性最高。鞘氨醇单胞菌属Sphingomonas是区分组间差异的关键标志物种，与假单胞菌属Pseudomonas呈显著正相关，且二者是N₁₀-甲酰基四氢叶酸生物合成代谢通路的主要构成菌属。结论蛇莓内生细菌群落在叶、果实与茎中相似性较高，与根差异明显；假单胞菌属与鞘氨醇单胞菌可能在次生代谢产物合成或宿主抗逆性增强中发挥协同作用。

关键词

蛇莓 / 内生细菌 / 高通量测序 / 多样性 / 群落结构

Abstract

收起

Objective To analyze the diversity and functional potential of endophytic bacterial communities in different tissues of Duchesnea indica and explore their potential relationship with the medicinal activity of the host plant. Methods Endophytic bacteria from the root, stem, leaf and fruit tissues of D. indica were studied. DNA fragments of the microbial communities were subjected to paired-end sequencing using Illumina high-throughput technology. Sequences were processed using DADA2 and Vsearch, followed by bioinformatics analysis and functional potential prediction. Results High-throughput sequencing of the endophytic bacterial genomes yielded a total of 1 914 360 raw sequences. At the phylum level, Proteobacteria was the dominant group. Among different tissues, the stem exhibited the highest richness of endophytic bacteria, while the root showed the highest diversity. Sphingomonas was identified as a key biomarker distinguishing intergroup differences and showed a significant positive correlation with Pseudomonas. Both bacterial genuses were major contributors to the N₁₀-formyltetrahydrofolate biosynthesis pathway. Conclusion The endophytic bacterial communities in the leaf, fruit and stem tissues of D. indica exhibited high similarity but were significantly distinct from those in the root. Pseudomonas and Sphingomonas may play synergistic roles in the synthesis of secondary metabolites or in enhancing host stress resistance.

Key words

Duchesnea indica(Andr.) Focke / endophytic bacteria / high-throughput sequencing / diversity / community structure

引用本文

张慧, 李贵文, 杨胜利, 朱李娜, 王靖宇, 肖功年. 基于Illumina高通量测序的蛇莓不同组织内生细菌多样性分析与功能预测. 中草药, 2026 , 57 (16) : 6510 -6520 . DOI: 10.7501/j.issn.0253-2670.2026.16.023

ZHANG Hui, LI Guiwen, YANG Shengli, ZHU Lina, WANG Jingyu, XIAO Gongnian. Diversity analysis and functional prediction of endophytic bacteria in different tissues of Duchesnea indica based on Illumina high-throughput sequencing[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (16) : 6510 -6520 . DOI: 10.7501/j.issn.0253-2670.2026.16.023

基金

收起

浙江省尖兵领雁研发攻关计划项目

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

Chen T, Chen M, Zhu R, et al. Study on antioxidant properties of Duchesnea indica Focke [J]. Strait Pharmaceut J, 2021, 33(3): 53-6.

段轲伟. 蛇莓中化学成分的分离与结构鉴定[D]. 郑州: 郑州大学, 2012.

许文东. 蛇莓的化学成分研究[D]. 沈阳: 沈阳药科大学, 2006.

赵萍. 蔷薇科植物蛇莓活性成分的化学研究[D]. 延吉: 延边大学, 2011.

乔芊芊. 蛇莓提取物体外抑菌、抗氧化、抗炎活性的研究[D]. 北京: 中国农业科学院, 2021.

马越鸣, 程能能. 蛇莓提取物对小鼠中枢神经系统的抑制作用[J]. 中草药, 1996, 37(9): 138-139.

彭博, 胡秦, 王立为, 等. 蛇莓总酚的抗肿瘤作用及免疫学机制的初步探讨[J]. 中国药理学通报, 2007, 23(8): 1007-1010.

Xiang B L, Yu X D, Li B, et al. Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke [J]. J Food Biochem, 2019, 43(7): e12899.

Peng B, Chang Q, Wang L W, et al. Suppression of human ovarian SKOV-3 cancer cell growth by Duchesnea phenolic fraction is associated with cell cycle arrest and apoptosis [J]. Gynecol Oncol, 2008, 108(1): 173-181.

Yang W E, Ho Y C, Tang C M, et al. Duchesnea indica extract attenuates oral cancer cells metastatic potential through the inhibition of the matrix metalloproteinase-2 activity by down-regulating the MEK/ERK pathway [J]. Phytomedicine, 2019, 63: 152960.

Aleynova O A, Nityagovsky N N, Suprun A R, et al. The diversity of fungal endophytes from wild grape Vitis amurensis Rupr [J]. Plants, 2022, 11(21): 2897.

Wilson D. Endophyte: The evolution of a term, and clarification of its use and definition [J]. Oikos, 1995, 73(2):274.

Stierle A, Strobel G, Stierle D. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew [J]. Science, 1993, 260(5105): 214-216.

Liu Y H, Liu W T, Liang Z S. Endophytic bacteria from Pinellia ternata, a new source of purine alkaloids and bacterial manure [J]. Pharm Biol, 2015, 53(10): 1545-1548.

Ding X W, Liu K H, Deng B W, et al. Isolation and characterization of endophytic fungi from Camptotheca acuminata [J]. World J Microbiol Biotechnol, 2013, 29(10):1831-1838.

Xu W F, Wang F, Zhang M, et al. Diversity of cultivable endophytic bacteria in mulberry and their potential for antimicrobial and plant growth-promoting activities [J]. Microbiol Res, 2019, 229: 126328.

Jia. Z, Zhang. X, Guo. G, et al. Isolation of endophytic fungi from wild licorice and their secondary metabolites activity [J]. J Chin Institute Food Sci Technol, 2022, 22(5): 320-31.

Callahan B J, McMurdie P J, Rosen M J, et al. DADA2: High-resolution sample inference from Illumina amplicon data [J]. Nat Methods, 2016, 13(7): 581-583.

Fitzpatrick A H, Rupnik A, O’Shea H, et al. Benchmarking bioinformatic tools for amplicon-based sequencing of norovirus [J]. Appl Environ Microbiol, 2023, 89(1): e01522-e01522.

Rimet F, Vasselon V, A Keszte B, et al. Do we similarly assess diversity with microscopy and high-throughput sequencing? Case of microalgae in lakes [J]. Org Divers Evol, 2018, 18(1): 51-62.

Bacaro G, Rocchini D, Ghisla A, et al. The spatial domain matters: Spatially constrained species rarefaction in a free and open source environment [J]. Ecol Complex, 2012, 12: 63-69.

Wang S M, Schneider D, Hartke T R, et al. Optimising high-throughput sequencing data analysis, from gene database selection to the analysis of compositional data: A case study on tropical soil nematodes [J]. Front Ecol Evol, 2024, 12: 1168288.

Rudar J, Porter T M, Wright M, et al. LANDMark: An ensemble approach to the supervised selection of biomarkers in high-throughput sequencing data [J]. BMC Bioinform, 2022, 23(1): 110.

Pérez-Losada M, Narayanan D B, Kolbe A R, et al. Comparative analysis of metagenomics and metataxonomics for the characterization of vermicompost microbiomes [J]. Front Microbiol, 2022, 13: 854423.

Cheng Y L, Zhao J, Liu Y Y, et al. Response of the endophytic microbiome in Cotinus coggygria roots to Verticillium wilt infection [J]. J Fungi, 2024, 10(11): 792.

Yuan Z S, Liu F, Yuan Y, et al. Structural composition and diversity of bacterial communities in high- and low-yielding moso bamboo forests [J]. Front Biosci (Landmark Ed), 2023, 28(11): 290.

Liu X, Cui J L, Zhou Z Y, et al. “Mini-community” simulation revealed the differences of endophytic fungal communities between the above- and below-ground tissues of Ephedra sinica Stapf [J]. Fungal Biol, 2023, 127(9): 1276-1283.

Liu F, Yuan Z S, Zhang X T, et al. Characteristics and diversity of endophytic bacteria in moso bamboo (Phyllostachys edulis) based on 16S rDNA sequencing [J]. Arch Microbiol, 2017, 199(9): 1259-1266.

胡连清, 陈露, 刘雯雯, 等. 酿酒专用糯红高粱内生细菌多样性分析及功能菌株筛选[J]. 作物杂志, 2024, 36(4): 194-202.

Chen T, Chen M, Zhu R, et al. Study on antioxidant properties of Duchesnea indica Focke [J]. Strait Pharmaceut J, 2021, 33(3): 53-6.
段轲伟. 蛇莓中化学成分的分离与结构鉴定[D]. 郑州: 郑州大学, 2012.
许文东. 蛇莓的化学成分研究[D]. 沈阳: 沈阳药科大学, 2006.
赵萍. 蔷薇科植物蛇莓活性成分的化学研究[D]. 延吉: 延边大学, 2011.
乔芊芊. 蛇莓提取物体外抑菌、抗氧化、抗炎活性的研究[D]. 北京: 中国农业科学院, 2021.
马越鸣, 程能能. 蛇莓提取物对小鼠中枢神经系统的抑制作用[J]. 中草药, 1996, 37(9): 138-139.
彭博, 胡秦, 王立为, 等. 蛇莓总酚的抗肿瘤作用及免疫学机制的初步探讨[J]. 中国药理学通报, 2007, 23(8): 1007-1010.
Xiang B L, Yu X D, Li B, et al. Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke [J]. J Food Biochem, 2019, 43(7): e12899.
Peng B, Chang Q, Wang L W, et al. Suppression of human ovarian SKOV-3 cancer cell growth by Duchesnea phenolic fraction is associated with cell cycle arrest and apoptosis [J]. Gynecol Oncol, 2008, 108(1): 173-181.
Yang W E, Ho Y C, Tang C M, et al. Duchesnea indica extract attenuates oral cancer cells metastatic potential through the inhibition of the matrix metalloproteinase-2 activity by down-regulating the MEK/ERK pathway [J]. Phytomedicine, 2019, 63: 152960.
Aleynova O A, Nityagovsky N N, Suprun A R, et al. The diversity of fungal endophytes from wild grape Vitis amurensis Rupr [J]. Plants, 2022, 11(21): 2897.
Wilson D. Endophyte: The evolution of a term, and clarification of its use and definition [J]. Oikos, 1995, 73(2):274.
Stierle A, Strobel G, Stierle D. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew [J]. Science, 1993, 260(5105): 214-216.
Liu Y H, Liu W T, Liang Z S. Endophytic bacteria from Pinellia ternata, a new source of purine alkaloids and bacterial manure [J]. Pharm Biol, 2015, 53(10): 1545-1548.
Ding X W, Liu K H, Deng B W, et al. Isolation and characterization of endophytic fungi from Camptotheca acuminata [J]. World J Microbiol Biotechnol, 2013, 29(10):1831-1838.
Xu W F, Wang F, Zhang M, et al. Diversity of cultivable endophytic bacteria in mulberry and their potential for antimicrobial and plant growth-promoting activities [J]. Microbiol Res, 2019, 229: 126328.
Jia. Z, Zhang. X, Guo. G, et al. Isolation of endophytic fungi from wild licorice and their secondary metabolites activity [J]. J Chin Institute Food Sci Technol, 2022, 22(5): 320-31.
Callahan B J, McMurdie P J, Rosen M J, et al. DADA2: High-resolution sample inference from Illumina amplicon data [J]. Nat Methods, 2016, 13(7): 581-583.
Fitzpatrick A H, Rupnik A, O’Shea H, et al. Benchmarking bioinformatic tools for amplicon-based sequencing of norovirus [J]. Appl Environ Microbiol, 2023, 89(1): e01522-e01522.
Rimet F, Vasselon V, A Keszte B, et al. Do we similarly assess diversity with microscopy and high-throughput sequencing? Case of microalgae in lakes [J]. Org Divers Evol, 2018, 18(1): 51-62.
Bacaro G, Rocchini D, Ghisla A, et al. The spatial domain matters: Spatially constrained species rarefaction in a free and open source environment [J]. Ecol Complex, 2012, 12: 63-69.
Wang S M, Schneider D, Hartke T R, et al. Optimising high-throughput sequencing data analysis, from gene database selection to the analysis of compositional data: A case study on tropical soil nematodes [J]. Front Ecol Evol, 2024, 12: 1168288.
Rudar J, Porter T M, Wright M, et al. LANDMark: An ensemble approach to the supervised selection of biomarkers in high-throughput sequencing data [J]. BMC Bioinform, 2022, 23(1): 110.
Pérez-Losada M, Narayanan D B, Kolbe A R, et al. Comparative analysis of metagenomics and metataxonomics for the characterization of vermicompost microbiomes [J]. Front Microbiol, 2022, 13: 854423.
Cheng Y L, Zhao J, Liu Y Y, et al. Response of the endophytic microbiome in Cotinus coggygria roots to Verticillium wilt infection [J]. J Fungi, 2024, 10(11): 792.
Yuan Z S, Liu F, Yuan Y, et al. Structural composition and diversity of bacterial communities in high- and low-yielding moso bamboo forests [J]. Front Biosci (Landmark Ed), 2023, 28(11): 290.
Liu X, Cui J L, Zhou Z Y, et al. “Mini-community” simulation revealed the differences of endophytic fungal communities between the above- and below-ground tissues of Ephedra sinica Stapf [J]. Fungal Biol, 2023, 127(9): 1276-1283.
Liu F, Yuan Z S, Zhang X T, et al. Characteristics and diversity of endophytic bacteria in moso bamboo (Phyllostachys edulis) based on 16S rDNA sequencing [J]. Arch Microbiol, 2017, 199(9): 1259-1266.
胡连清, 陈露, 刘雯雯, 等. 酿酒专用糯红高粱内生细菌多样性分析及功能菌株筛选[J]. 作物杂志, 2024, 36(4): 194-202.

2026年第57卷第16期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.7501/j.issn.0253-2670.2026.16.023

接收时间：2026-02-02
首发时间：2026-10-22
出版时间：2026-08-28

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2026-02-02

基金

浙江省尖兵领雁研发攻关计划项目

作者信息

^1.浙江科技大学生物与化学工程学院, 浙江杭州 310023

^2.浙江工业大学药学院, 浙江杭州 310014

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.16.023

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT