Article(id=1304388228967064253, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388157621948709, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.15.014, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1772208000000, receivedDateStr=2026-02-28, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788919988146, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788919988146, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788919988146, creator=13701087609, updateTime=1788919988146, updator=13701087609, issue=Issue{id=1304388157621948709, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='15', pageStart='5789', pageEnd='6208', issueExtLink='null', onlineDate='null', pubDate='1786464000000', pubDateStr='2026-08-12', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1788919971137, creator='13701087609', updateTime=1788923514106, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304403017982300207, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388157621948709, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304403017982300208, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388157621948709, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=5942, endPage=5952, ext={EN=ArticleExt(id=1304388229352940223, articleId=1304388228967064253, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Protective effect and mechanism of soybean isoflavones on ischemic stroke, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective To study the protective effect and potential mechanism of soybean isoflavones (SI) on ischemic stroke. Methods SI were isolated from soybeans and components were analyzed. An oxygen glucose deprivation/reperfusion (OGD/R)-induced PC12 cells model was constructed to investigate the effect of SI on cell survival, migration ability, apoptosis, intracellular Ca2+ , reactive oxygen species (ROS), lactate dehydrogenase (LDH), cysteine aspartate protease-3 (Caspase-3) levels, and expressions of Kelch-like ECH associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2) pathway related proteins. A rat model of global cerebral ischemia-reperfusion injury was established using bilateral common carotid artery ligation. The effect of SI on neurological function score, brain tissue water content, proportion of cerebral infarction volume, pathological changes, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), LDH activities, malondialdehyde (MDA) level, and expressions of Keap1/Nrf2 pathway related proteins in brain tissue were investigated. Results In vitro experiments showed that compared with model group, SI significantly increased cell survival rate and migration rate (P < 0.05, 0.01), decreased cell apoptosis rate and intracellular Ca2+ , ROS, LDH, Caspase-3 levels (P < 0.05, 0.01), upregulated Nrf2, heme oxygenase-1 (HO-1), NAD(P)H quinone oxidoreductase 1 (NQO1) protein expressions (P < 0.01), and downregulated Keap1 protein expression (P < 0.05, 0.01). In vivo experiments, compared with model group, SI significantly improved neurological deficits, reduced brain edema and infarct volume in rats (P < 0.01), repaired brain tissue pathological damage, reduced MDA level and LDH activity in brain tissue (P < 0.01), increased SOD, CAT and GSH-Px activities in brain tissue (P < 0.01), upregulated Nrf2, HO-1 and NQO1 protein expressions in brain tissue (P < 0.01), and downregulated Keap1 protein expression in brain tissue (P < 0.01). Conclusion SI have a significant neuroprotective effect on ischemic stroke, which may be related to the activation of Keap1/Nrf2 pathway, enhancing the antioxidant capacity of body, inhibiting oxidative stress damage, reducing neuronal apoptosis and alleviating brain tissue damage., authors=ZHANG Yue, JIN Chang, LI Bing, CHENG Zhifeng, WANG Jing, ZHENG Meizhu, authorsList=ZHANG Yue, JIN Chang, LI Bing, CHENG Zhifeng, WANG Jing, ZHENG Meizhu, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304388229210333886, articleId=1304388228967064253, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=大豆异黄酮对缺血性脑卒中的保护作用及机制, columnId=1304140191707456168, journalTitle=中草药, columnName=药理与临床, runingTitle=null, highlight=null, articleAbstract=目的 研究大豆异黄酮对缺血性脑卒中的保护作用及潜在作用机制。方法 从大豆Glycine max 中分离大豆异黄酮并进行成分分析。构建氧糖剥夺/再灌注（oxygen glucose deprivation/reperfusion，OGD/R）诱导的PC12细胞模型，考察大豆异黄酮对细胞存活率、迁移能力、凋亡及细胞内Ca²⁺、活性氧（reactive oxygen species，ROS）、乳酸脱氢酶（lactate dehydrogenase，LDH）、半胱氨酸天冬氨酸蛋白酶-3（cystein-asparate protease-3，Caspase-3）水平和Kelch样环氧氯丙烷相关蛋白1（Kelch-like ECH-associated protein 1，Keap1）/核因子E2相关因子2（nuclear factor erythroid 2-related factor 2，Nrf2）通路相关蛋白表达的影响。采用双侧颈总动脉结扎法建立大鼠全脑缺血再灌注损伤模型，考察大豆异黄酮对神经功能评分、脑组织含水量、脑梗死体积占比、脑组织病理变化及脑组织超氧化物歧化酶（superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）、过氧化氢酶（catalase，CAT）、LDH活性、丙二醛（malondialdehyde，MDA）水平和Keap1/Nrf2通路相关蛋白表达的影响。结果 体外实验结果显示，与模型组比较，大豆异黄酮显著提高细胞存活率和迁移率（P ＜0.05、0.01），降低细胞凋亡率和细胞内Ca²⁺、ROS、LDH、Caspase-3水平（P ＜0.05、0.01），上调Nrf2、血红素加氧酶-1（heme oxygenase-1，HO-1）、醌氧化还原酶1[NAD(P)H quinone oxidoreductase 1，NQO1]的蛋白表达（P ＜0.01），下调Keap1蛋白表达（P ＜0.05、0.01）。体内实验结果显示，与模型组比较，大豆异黄显著改善大鼠神经功能缺损、减轻脑水肿及梗死体积（P ＜0.01），修复脑组织病理损伤，降低脑组织内MDA水平和LDH活性（P ＜0.01），提高脑组织内SOD、CAT和GSH-Px活性（P ＜0.01），上调脑组织Nrf2、HO-1和NQO1蛋白表达（P ＜0.01），下调脑组织Keap1蛋白表达（P ＜0.01）。结论 大豆异黄酮对缺血性脑卒中有显著的神经保护作用，其机制可能与通过激活Keap1/Nrf2通路，增强机体抗氧化能力、抑制氧化应激损伤、减少神经细胞凋亡及减轻脑组织损伤有关。, authors=张月1 , 金畅1 , 李冰1 , 程志锋1 , 王晶2 , 郑梅竹2 , authorsList=张月, 金畅, 李冰, 程志锋, 王晶, 郑梅竹, authorCompany=1 长春师范大学生命科学学院, 吉林 长春 130032; 1-42 诱导的海马神经元炎症和凋亡[J]. 中国药理学通报, 2022, 38(6): 874-879.2 O2 诱导的PC12细胞损伤的作用机制[J]. 中医药学报, 2025, 53(1): 20-28.2+ 信号通路减轻大鼠脑缺血再灌注引起的钙超载[J]. 南方医科大学学报, 2024, 44(6): 1048-1058.
中草药
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2026
, 57
(15) :
5942
-5952
大豆异黄酮对缺血性脑卒中的保护作用及机制
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张月, 金畅, 李冰, 程志锋, 王晶, 郑梅竹
作者信息
通讯作者:
郑梅竹
作者简介:
张月: 张月，硕士研究生，从事天然产物及神经退行性疾病的研究。E-mail:15942907284@163.com
Protective effect and mechanism of soybean isoflavones on ischemic stroke
ZHANG Yue, JIN Chang, LI Bing, CHENG Zhifeng, WANG Jing, ZHENG Meizhu
Affiliations
doi: 10.7501/j.issn.0253-2670.2026.15.014
文章导航
目的 研究大豆异黄酮对缺血性脑卒中的保护作用及潜在作用机制。方法 从大豆Glycine max 中分离大豆异黄酮并进行成分分析。构建氧糖剥夺/再灌注（oxygen glucose deprivation/reperfusion，OGD/R）诱导的PC12细胞模型，考察大豆异黄酮对细胞存活率、迁移能力、凋亡及细胞内Ca²⁺、活性氧（reactive oxygen species，ROS）、乳酸脱氢酶（lactate dehydrogenase，LDH）、半胱氨酸天冬氨酸蛋白酶-3（cystein-asparate protease-3，Caspase-3）水平和Kelch样环氧氯丙烷相关蛋白1（Kelch-like ECH-associated protein 1，Keap1）/核因子E2相关因子2（nuclear factor erythroid 2-related factor 2，Nrf2）通路相关蛋白表达的影响。采用双侧颈总动脉结扎法建立大鼠全脑缺血再灌注损伤模型，考察大豆异黄酮对神经功能评分、脑组织含水量、脑梗死体积占比、脑组织病理变化及脑组织超氧化物歧化酶（superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）、过氧化氢酶（catalase，CAT）、LDH活性、丙二醛（malondialdehyde，MDA）水平和Keap1/Nrf2通路相关蛋白表达的影响。结果 体外实验结果显示，与模型组比较，大豆异黄酮显著提高细胞存活率和迁移率（P ＜0.05、0.01），降低细胞凋亡率和细胞内Ca²⁺、ROS、LDH、Caspase-3水平（P ＜0.05、0.01），上调Nrf2、血红素加氧酶-1（heme oxygenase-1，HO-1）、醌氧化还原酶1[NAD(P)H quinone oxidoreductase 1，NQO1]的蛋白表达（P ＜0.01），下调Keap1蛋白表达（P ＜0.05、0.01）。体内实验结果显示，与模型组比较，大豆异黄显著改善大鼠神经功能缺损、减轻脑水肿及梗死体积（P ＜0.01），修复脑组织病理损伤，降低脑组织内MDA水平和LDH活性（P ＜0.01），提高脑组织内SOD、CAT和GSH-Px活性（P ＜0.01），上调脑组织Nrf2、HO-1和NQO1蛋白表达（P ＜0.01），下调脑组织Keap1蛋白表达（P ＜0.01）。结论 大豆异黄酮对缺血性脑卒中有显著的神经保护作用，其机制可能与通过激活Keap1/Nrf2通路，增强机体抗氧化能力、抑制氧化应激损伤、减少神经细胞凋亡及减轻脑组织损伤有关。
缺血性脑卒中
/
大豆异黄酮
/
Keap1/Nrf2通路
/
氧化应激
/
凋亡
/
大豆苷
/
黄豆黄苷
/
染料木苷
/
乙酰黄豆苷
/
乙酰基苷
/
丙二酰染料木苷
/
乙酰染料木苷
Objective To study the protective effect and potential mechanism of soybean isoflavones (SI) on ischemic stroke. Methods SI were isolated from soybeans and components were analyzed. An oxygen glucose deprivation/reperfusion (OGD/R)-induced PC12 cells model was constructed to investigate the effect of SI on cell survival, migration ability, apoptosis, intracellular Ca2+ , reactive oxygen species (ROS), lactate dehydrogenase (LDH), cysteine aspartate protease-3 (Caspase-3) levels, and expressions of Kelch-like ECH associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2) pathway related proteins. A rat model of global cerebral ischemia-reperfusion injury was established using bilateral common carotid artery ligation. The effect of SI on neurological function score, brain tissue water content, proportion of cerebral infarction volume, pathological changes, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), LDH activities, malondialdehyde (MDA) level, and expressions of Keap1/Nrf2 pathway related proteins in brain tissue were investigated. Results In vitro experiments showed that compared with model group, SI significantly increased cell survival rate and migration rate (P < 0.05, 0.01), decreased cell apoptosis rate and intracellular Ca2+ , ROS, LDH, Caspase-3 levels (P < 0.05, 0.01), upregulated Nrf2, heme oxygenase-1 (HO-1), NAD(P)H quinone oxidoreductase 1 (NQO1) protein expressions (P < 0.01), and downregulated Keap1 protein expression (P < 0.05, 0.01). In vivo experiments, compared with model group, SI significantly improved neurological deficits, reduced brain edema and infarct volume in rats (P < 0.01), repaired brain tissue pathological damage, reduced MDA level and LDH activity in brain tissue (P < 0.01), increased SOD, CAT and GSH-Px activities in brain tissue (P < 0.01), upregulated Nrf2, HO-1 and NQO1 protein expressions in brain tissue (P < 0.01), and downregulated Keap1 protein expression in brain tissue (P < 0.01). Conclusion SI have a significant neuroprotective effect on ischemic stroke, which may be related to the activation of Keap1/Nrf2 pathway, enhancing the antioxidant capacity of body, inhibiting oxidative stress damage, reducing neuronal apoptosis and alleviating brain tissue damage.
ischemic stroke
/
soybean isoflavones
/
Keap1/Nrf2 pathway
/
oxidative stress
/
apoptosis
/
daidzin
/
glycitin
/
genistin
/
acetyl daidzin
/
acetyl glycoside
/
malonyl genistin
/
acetyl genisti
张月, 金畅, 李冰, 程志锋, 王晶, 郑梅竹.
大豆异黄酮对缺血性脑卒中的保护作用及机制.
中草药,
2026
, 57
(15)
: 5942
-5952
.
DOI: 10.7501/j.issn.0253-2670.2026.15.014
ZHANG Yue, JIN Chang, LI Bing, CHENG Zhifeng, WANG Jing, ZHENG Meizhu.
Protective effect and mechanism of soybean isoflavones on ischemic stroke[J].
Chinese Traditional and Herbal Drugs ,
2026
, 57
(15)
: 5942
-5952
.
DOI: 10.7501/j.issn.0253-2670.2026.15.014
参考文献
引证文献
Li L, Sun L L, Qiu Y, et al. Protective effect of stachydrine against cerebral ischemia-reperfusion injury by reducing inflammation and apoptosis through p65 and JAK2/STAT3 signaling pathway [J]. Front Pharmacol, 2020, 11: 64.1-42 诱导的海马神经元炎症和凋亡[J]. 中国药理学通报, 2022, 38(6): 874-879.2 O2 诱导的PC12细胞损伤的作用机制[J]. 中医药学报, 2025, 53(1): 20-28.2+ 信号通路减轻大鼠脑缺血再灌注引起的钙超载[J]. 南方医科大学学报, 2024, 44(6): 1048-1058.
2026年第57卷第15期
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doi: 10.7501/j.issn.0253-2670.2026.15.014
接收时间：2026-02-28
首发时间：2026-09-09
https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.15.014
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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