中草药

负载姜黄素可打印氨基酸基低共熔凝胶敷料制备及其体外性能和治疗口腔溃疡药效作用研究

PDF下载

中草药 | 药剂与工艺 2026,57(15): 5874-5889

收起

中草药 |药剂与工艺 2026 , 57 (15) : 5874 -5889

负载姜黄素可打印氨基酸基低共熔凝胶敷料制备及其体外性能和治疗口腔溃疡药效作用研究

全屏

关晓朵¹, 严文政¹, 李惠杰¹, 杨根生¹, 杨庆良¹, 贺悦²

作者信息

通讯作者:

杨庆良

作者简介:

关晓朵: 关晓朵，女，硕士研究生，研究方向为药物新剂型。Tel:13458456645 E-mail:221125070253@zjut.edu.cn

Preparation, in vitro performance, and therapeutic efficacy against oral ulcers of curcumin-loaded printable amino acid-based deep eutectic gel dressings

GUAN Xiaoduo, YAN Wenzheng, LI Huijie, YANG Gensheng, YANG Qingliang, HE Yue

Affiliations

doi: 10.7501/j.issn.0253-2670.2026.15.009

文章导航

摘要

收起

目的制备基于氨基酸的低共熔凝胶敷料，对其体外性能及体内治疗口腔溃疡药效作用进行研究。方法以赖氨酸、脯氨酸、精氨酸、甘氨酸为氢键供体，乳酸、柠檬酸、甘油、甘露醇、苹果酸为氢键受体，制备筛选低共熔溶剂（deep eutectic solvent，DES），以姜黄素溶解度为指标确定最优体系。基于甲基丙烯酰化明胶/甲基丙烯酰化壳聚糖（gelatin methacryloyl/chitosan methacryloyl，GelMA/CSMA）复合基质，结合3D打印技术优化处方工艺制备凝胶敷料，评价其机械性能、黏附性、溶胀特性和生物相容性，并构建SD大鼠口腔溃疡模型验证体内疗效。结果最优DES体系（脯氨酸-乳酸1∶12）对姜黄素的增溶能力达0.789 mg/mL，较水中溶解度提升700余倍。最优打印处方和工艺参数分别为1% CSMA-5% GelMA，0.1%苯基-2,4,6-三甲基苯甲酰基膦酸锂（lithium phenyl-2,4,6-trimethylbenzoylphosphinate，LAP），0.02%柠檬黄，20% DES添加量，100 μm打印层高，25 s逐层曝光时间。所得氨基酸基凝胶敷料机械性能优异，黏附力25 min达2.5 N，6 h溶胀率约500%。体外生物相容性显示，L929细胞存活率均＞90%，DES体系无显著细胞毒性；SD大鼠口腔溃疡模型中，该凝胶可局部缓释姜黄素，使溃疡面积较对照组减少40%，并有效降低中性粒细胞密度及炎症浸润程度。结论成功制备负载姜黄素的氨基酸基DES凝胶敷料，具有良好的伤口适配性与精准药物释放行为，为口腔溃疡提供了高效、安全的治疗策略。

关键词

低共熔溶剂 / 氨基酸 / 姜黄素 / 凝胶敷料 / 口腔溃疡

Abstract

收起

Objective To prepare an amino acid-based deep eutectic gel dressing and study in vitro performance and the in vivo therapeutic effect on oral ulcer. Methods Deep eutectic solvents (DESs) were screened using lysine, proline, arginine, and glycine as hydrogen bond donors, and lactic acid, citric acid, glycerol, mannitol, and malic acid as acceptors, with curcumin solubility identifying the optimal system. Gel dressings were prepared by optimizing the formulation process based on the composite matrix of gelatin methacryloyl/chitosan methacryloyl (GelMA/CSMA) in combination with 3D printing technology. Its mechanical properties, adhesiveness, swelling, and biocompatibility were evaluated, and in vivo efficacy was verified in an SD rat oral ulcer model. Results The optimal DES system (proline-lactic acid, 1:12) increased curcumin solubility over 700-fold (0.789 mg/mL) compared to its solubility in water. The optimal printing formulation and process parameters were as follows: 1% CSMA, 5% GelMA, 0.1% lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP), 0.02% tartrazine, 20% DES content, 100 μm layer height, and a layer-by-layer exposure time of 25 s. The obtained amino acid-based gel dressing had excellent mechanical properties, with a 2.5 N adhesion force at 25 min and an approximately 500% swelling ratio at 6 h. In vitro biocompatibility showed that the survival rate of L929 cells was all above 90%, and the DES system had no significant cytotoxicity. It also showed high in vitro biocompatibility (L929 viability > 90%, non-cytotoxic). In vivo, the gel achieved localized, sustained curcumin release in SD oral ulcer model rats, reducing ulcer area by 40% with decreased neutrophil density and inflammatory infiltration. Conclusion Successfully prepared an amino acid-based DES gel dressing loaded with curcumin, which exhibits excellent wound adaptability and precise drug release behavior, offering an efficient and safe therapeutic strategy for oral ulcers.

Key words

deep eutectic solvents / amino acids / curcumin / gel dressings / oral ulcer

引用本文

关晓朵, 严文政, 李惠杰, 杨根生, 杨庆良, 贺悦. 负载姜黄素可打印氨基酸基低共熔凝胶敷料制备及其体外性能和治疗口腔溃疡药效作用研究. 中草药, 2026 , 57 (15) : 5874 -5889 . DOI: 10.7501/j.issn.0253-2670.2026.15.009

GUAN Xiaoduo, YAN Wenzheng, LI Huijie, YANG Gensheng, YANG Qingliang, HE Yue. Preparation, in vitro performance, and therapeutic efficacy against oral ulcers of curcumin-loaded printable amino acid-based deep eutectic gel dressings[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (15) : 5874 -5889 . DOI: 10.7501/j.issn.0253-2670.2026.15.009

基金

收起

湖州市科技局公益研究项目—人口健康类 (2025GY121); 浙江省中医药科技计划项目资助 (2025ZL027)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

Yu W W, Li X, Liu X F, et al. Spermidine-functionalized Janus hydrogel microneedles inhibit ferroptosis and promote healing of oral ulcers [J]. Bioact Mater, 2026, 60: 299-319.
Qiao Z, Zhang K L, Liu H F, et al. CSMP: A self-assembled plant polysaccharide-based hydrofilm for enhanced wound healing [J]. Adv Healthc Mater, 2024, 13(6): 2303244.
Liu T F, Lu Y F, Zhan R X, et al. Nanomaterials and nanomaterials-based drug delivery to promote cutaneous wound healing [J]. Adv Drug Deliv Rev, 2023, 193: 114670.
Dong R N, Guo B L. Smart wound dressings for wound healing [J]. Nano Today, 2021, 41: 101290.
Jin X Z, Duan J J, et al. Multifunctional plasma-activated alginate dressing potentiates the moist healing of chronic infected wounds [J]. ACS Appl Mater Interfaces, 2025, 17(34): 48111-48122.
Minsart M, Van Vlierberghe S, Dubruel P, et al. Commercial wound dressings for the treatment of exuding wounds: An in-depth physico-chemical comparative study [J]. Burns Trauma, 2022, 10: tkac024.
Vessally E, Rovnag R. Recent advances in polysaccharide-based nanocomposites as wound healing and transdermal drug delivery systems: A review [J]. Int J Biol Macromol, 2025, 321: 144743.
Lu Z J, Sun C, Hu P Y, et al. pH-responsive multifunctional drug-loaded covalent organic framework hydrogel dressing for enhanced bacteria-infected wound healing [J]. Mater Des, 2025, 257: 114487.
Qiao L P, Liang Y P, Chen J Y, et al. Antibacterial conductive self-healing hydrogel wound dressing with dual dynamic bonds promotes infected wound healing [J]. Bioact Mater, 2023, 30: 129-141.
Jiang L, Wang X M, Jiang H. Versatile chitosan-based hydrogel dressings for multi-scenario wound management [J]. Small, 2026, 22(14): e14813.
Luo X Z, Cheng S W, Zhang W, et al. Near-infrared fluorescence probe for indication of the pathological stages of wound healing process and its clinical application [J]. ACS Sens, 2024, 9(2): 810-819.
Zhu M, Ou J Z, Chen Y Z, et al. Programming of macrophage polarization in different stages for accelerating wound healing [J]. Chem Eng J, 2024, 491: 152131.
Shen J Y, Tong Z, Han B, et al. Synergistic wound healing: Unraveling the multi-target effects of traditional Chinese medicine and its biomaterials on chronic wound pathways [J]. Int J Nanomed, 2025, 20: 12889-12912.
冯雪, 朱双益, 胡溪, 等. HIF-1α/VEGFR2/ERK1/2通路在姜黄素治疗大鼠腹膜透析相关腹膜纤维化的作用[J]. 中国药学杂志, 2025, 60(21): 2285-2292.
Bertoncini-Silva C, Vlad A, Ricciarelli R, et al. Enhancing the bioavailability and bioactivity of curcumin for disease prevention and treatment [J]. Antioxidants, 2024, 13(3): 331.
赵笑雨, 陈怡莹, 张海亮, 等. 共载姜黄素及顺铂pH敏感脂质体制备及其体外抗乳腺癌活性评价[J]. 中草药, 2025, 56(1): 52-67.
罗益蓉, 王隽, 朱宗萍, 等. 姜黄多糖为稳定剂制备口服姜黄素纳米乳及其抗炎活性研究[J]. 中草药, 2025, 56(6): 1935-1944.
Meng K X, Tu X Z, Sun F X, et al. Carbohydrate polymer-based nanoparticles in curcumin delivery for cancer therapy [J]. Int J Biol Macromol, 2025, 304: 140441.
Liu J H, Zhang Z P, Lin X F, et al. Magnesium metal-organic framework microneedles loaded with curcumin for accelerating oral ulcer healing [J]. J Nanobiotechnol, 2024, 22(1): 594.
Guo X L, Yang L, Sheng N, et al. Antibacterial antioxidant polyurethane/curcumin patch with anti-swelling ability for tissue adhesion and treatment of oral ulcers [J]. Chem Eng J, 2025, 511: 162131.
梁佩云, 黄钰景, 陈浩铭, 等. 超声辅助低共熔溶剂提取莱菔子中萝卜硫素的工艺优化及其铜绿假单胞菌生物膜抑制活性研究[J]. 中草药, 2024, 55(6): 1960-1969.
Han W F, Yuan M H, Fan Z L, et al. Optimization of CO₂ capturing performance of amino acid-based deep eutectic solvent phase change absorbents: Introducing DBN into HBA [J]. Sep Purif Technol, 2025, 354: 128798.
Parsana N, El Seoud O, Al-Ghamdi A, et al. Deep eutectic solvent and poly(vinyl alcohol) based self-healable, injectable and adhesive “Eutectogel”: An emerging drug delivery vehicle [J]. Chem Select, 2024, 9(1): e202304157.
Yang L, Li W Z, Ding X Y, et al. Biomimetic mineralized organic-inorganic hybrid scaffolds from microfluidic 3D printing for bone repair [J]. Adv Funct Mater, 2025, 35(1): 2410927.
Shinde S, Kim K H, Pawar O, et al. 3D-printable alginate-MoS₂-AgNW hydrogel bioink for dual-mode wearable capacitive biosensors [J]. Int J Bioprinting, 2025, 11(5): 301-314.

2026年第57卷第15期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.7501/j.issn.0253-2670.2026.15.009

接收时间：2026-02-16
首发时间：2026-09-09

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2026-02-16

基金

作者信息

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.15.009

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT