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Method Compounds were isolated and purified by MCI gel, silica gel, Toyopearl HW-40C, ODS and semi-preparative HPLC. Their chemical structures were comprehensively elucidated by spectroscopic methods, such as HRESIMS, NMR, IR, UV and ECD. Results A total of 14 nitrogen-containing compounds were finally isolated and identified as 6-[(8R,9S)-8,9,10-trihydroxybutyl]-3,4-dimethylpyridazine (1), 6-ethyl-3-(hydroxymethyl)-1H-pyridin-2-one (2), (+)-(1R,3R)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (3), 1H-indole-3-carboxaldehyde (4), cyclo-(L-Leu-L-Phe) (5), cyclo-(L-Phe-L-Ile) (6), cyclo-(L-Asn-L-Phe) (7), (3S,6S)-3-benzyl-6-(1-hydroxyethyl)-2,5-piperazinedione (8), cyclo-(D-Phe-Gly) (9), cyclo-(L-Tyr-L-Tyr) (10), cyclo-(L-Val-L-Tyr) (11), pedatisectine L (12), cyclo-(Ala-Tyr) (13), and (2S,5S)-5-[(1S)-1-methylpropyl]-3,6-dioxo-2-piperazinepropanoic acid (14). Conclusion Compounds 1 and 2 were characterized as two new compounds named cyrtoneine J and cyrtoneine K, while compounds 3 and 5—14 were isolated from the genus Polygonatum for the first time., authors=MA Xudong, ZHANG Junyang, YANG Meng, HAO Zhiyou, ZHENG Xiaoke, FENG Weisheng, authorsList=MA Xudong, ZHANG Junyang, YANG Meng, HAO Zhiyou, ZHENG Xiaoke, FENG Weisheng, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304735427689213961, articleId=1304735427454332936, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=九蒸九晒多花黄精中2个新的含氮类化合物, columnId=1304140187169214944, journalTitle=中草药, columnName=化学成分, runingTitle=null, highlight=null, articleAbstract=目的 研究九蒸九晒多花黄精Polygonatum cyrtonema 70%丙酮提取物的化学成分。方法 采用MCI、硅胶、Toyopearl HW-40C、ODS以及半制备液相等多种色谱技术进行分离纯化，通过HRESIMS、NMR、IR、UV、ECD等波谱学方法进行结构鉴定。结果 从九蒸九晒多花黄精70%丙酮提取物中分离得到14个含氮类化合物，分别鉴定为6-[(8R,9S)-8,9,10-三羟基丁基]-3,4-二甲基哒嗪（1）、6-乙基-3-羟甲基-1H-吡啶-2-酮（2）、(＋)-(1R,3R)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid（3）、3-吲哚甲醛（4）、环(L-亮氨酸-L-苯丙氨酸)（5）、环(L-苯丙氨酸-L-异亮氨酸)（6）、环(天冬酰胺-苯丙氨酸)（7）、(3S,6S)-3-苄基-6-(1-羟乙基)-2,5-哌嗪二酮（8）、环(D-苯丙-苷)二肽（9）、环(酪-酪)二肽（10）、环(L-缬-L-酪) 二肽（11）、pedatisectine L（12）、环(丙-酪) 二肽（13）、(2S,5S)-5-[(1S)-1-methylpropyl]-3,6-dioxo-2-piperazine propanoic acid（14）。结论 化合物1和2为新化合物，命名为多花黄精碱J和多花黄精碱K；化合物3、5～14均为首次从黄精中分离得到。, authors=马旭东^1,2, 张俊阳^1,2, 杨梦^1,2, 郝志友^1,2, 郑晓珂^1,2, 冯卫生^1,2, authorsList=马旭东, 张俊阳, 杨梦, 郝志友, 郑晓珂, 冯卫生, authorCompany=1 河南中医药大学药学院, 河南 郑州 450046;
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