Article(id=1304388193705550400, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388135723496407, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.13.017, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1765123200000, receivedDateStr=2025-12-08, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788919979740, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788919979740, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788919979740, creator=13701087609, updateTime=1788919979740, updator=13701087609, issue=Issue{id=1304388135723496407, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='13', pageStart='4949', pageEnd='5352', issueExtLink='null', onlineDate='null', pubDate='1783785600000', pubDateStr='2026-07-12', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788919965916, creator='13701087609', updateTime=1788923489765, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304402915871977875, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388135723496407, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304402915871977876, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388135723496407, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=5140, endPage=5147, ext={EN=ArticleExt(id=1304388194007540290, articleId=1304388193705550400, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Effect and mechanism of usnic acid on inflammatory injury in mice with cerebral hemorrhage based on HMGB1/RAGE pathway, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective To explore the effect of usnic acid on inflammatory injury in mice with intracerebral hemorrhage (ICH) based on high mobility group box 1 (HMGB1)/receptor for advanced glycation end products (RAGE) pathway. Methods C57BL/6 mice were randomly divided into sham group, model group, HMGB1 inhibitor ethyl pyruvate (40 mg/kg) group, usnic acid low-, high-dose (20, 30 mg/kg) groups and usnic acid (30 mg/kg) + rHMGB1 (8 μg/kg) group, with 18 mice in each group. The ICH model was constructed by intracranial injection of 0.5 μL collagenase. After continuous administration for 3 d, the modified neurological severity score (mNSS) and left turn rate of mice in each group were evaluated. The water rate of brain tissue in mice was determined. ELISA was used to measure the levels of interleukin-6 (IL-6), IL-1β and IL-18 in brain tissue. Hematoxylin-eosin (HE) staining was used to observe pathological changes in brain tissue. Immunofluorescence was used to observe microglia activation in brain tissue. TUNEL staining was used to detect cell apoptosis of brain tissue. Western blotting was used to detect the expressions of HMGB1/RAGE pathway related proteins in brain tissue. Results Compared with sham group, the striatum tissue of mice in model group was severely damaged, mNSS, left cerebral hemisphere water rate, ionized calcium-binding adapter molecule 1 (Iba1) positive cells number, inflammatory factors levels, cell apoptosis rate, HMGB1, RAGE, phosphorylated nuclear factor-κB p65 (p-NF-κB p65)/NF-κB p65 and TNF-α protein expression levels were significantly increased (P < 0.05), and the left turn rate was significantly decreased (P < 0.05). Compared with model group, the damage degree of striatum tissue in usnic acid low-, high-dose group and ethyl pyruvate group was alleviated (P < 0.05), mNSS, left cerebral hemisphere water rate, Iba1 positive cells number, inflammatory factors levels, cell apoptosis rate and HMGB1/RAGE pathway related proteins expression levels were significantly decreased (P < 0.05), and the left turn rate was significantly increased (P < 0.05). rHMGB1 significantly reversed the inhibitory effect of usnic acid on inflammatory injury in ICH mice (P < 0.05). Conclusion Usnic acid may alleviate inflammatory injury in ICH mice by inhibiting HMGB1/RAGE pathway., authors=WU Junxiao, LI Yan, SHI Bo, CHEN Hong, HU Chengwang, authorsList=WU Junxiao, LI Yan, SHI Bo, CHEN Hong, HU Chengwang, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304388193940431425, articleId=1304388193705550400, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=基于HMGB1/RAGE通路探究松萝酸对脑出血小鼠炎症损伤的影响及作用机制, columnId=1304140191707456168, journalTitle=中草药, columnName=药理与临床, runingTitle=null, highlight=null, articleAbstract=目的 基于高迁移率族蛋白B1（high mobility group box 1，HMGB1）/晚期糖基化终末产物受体（receptor for advanced glycation end products，RAGE）通路探究松萝酸对脑出血（intracerebral hemorrhage，ICH）小鼠炎症损伤的影响。方法 将C57BL/6小鼠随机分为假手术组、模型组、HMGB1抑制剂丙酮酸乙酯（40 mg/kg）组及松萝酸低、高剂量（20、30 mg/kg）组和松萝酸（30 mg/kg）＋rHMGB1（8 μg/kg）组，每组18只。采用颅内注射0.5μL胶原酶构建ICH模型，连续3 d给药后，评价各组小鼠改良神经功能缺损评分（modified neurological severity score，mNSS）和左转率；测定小鼠脑组织含水率；ELISA检测脑组织白细胞介素-6（interleukin-6，IL-6）、IL-1β和IL-18水平；苏木素-伊红（hematoxylin-eosin，HE）染色观察脑组织病理改变；免疫荧光观察脑组织小胶质细胞活化情况；TUNEL染色检测脑组织细胞凋亡；Western blotting检测脑组织HMGB1/RAGE通路相关蛋白表达。结果 与假手术组比较，模型组小鼠纹状体组织损伤严重，mNSS、左侧大脑半球含水率、离子化钙结合适配分子1-（ionized calcium-binding adapter molecule 1，Iba1）阳性细胞数、炎症因子水平、细胞凋亡率及HMGB1、RAGE、磷酸化核因子-κB p65（phosphorylated nuclear factor-κB p65，p-NF-κB p65）/NF-κB p65、肿瘤坏死因子-α（tumor necrosis-α，TNF-α）蛋白表达水平显著升高（P ＜0.05），左转率显著降低（P ＜0.05）；与模型组比较，松萝酸低、高剂量组和丙酮酸乙酯组纹状体组织损伤程度减轻，mNSS、左侧大脑半球含水率、Iba1阳性细胞数、炎症因子水平、细胞凋亡率及HMGB1/RAGE通路相关蛋白表达水平显著降低（P ＜0.05），左转率显著升高（P ＜0.05）；rHMGB1显著逆转了松萝酸对ICH小鼠炎症损伤的抑制作用（P ＜0.05）。结论 松萝酸可能通过抑制HMGB1/RAGE通路减轻ICH小鼠炎症损伤。, authors=吴俊晓1 , 李艳2 , 史博3 , 陈红4 , 胡成旺3 , authorsList=吴俊晓, 李艳, 史博, 陈红, 胡成旺, authorCompany=1 南阳医学高等专科学校 康复医学系, 河南 南阳 473061; et al . Sex differences in treatment, radiological features and outcome after intracerebral haemorrhage: Pooled analysis of intensive blood pressure reduction in acute cerebral haemorrhage trials 1 and 2 [J]. Eur Stroke J , 2020, 5(4): 345-350.et al . Effects of Xingnaojing on serum high-sensitivity C-reactive protein and neuron-specific enolase in patients with acute cerebral hemorrhage: A protocol of systematic review and Meta-analysis [J]. Medicine , 2020, 99(45): e21379.JAMA , 2019, 321(13): 1295-1303.J Stroke Cerebrovasc Dis , 2022, 31(9): 106637.et al . Lichen-derived compounds and extracts as biologically active substances with anticancer and neuroprotective properties [J]. Pharmaceuticals , 2021, 14(12): 1293.et al . Usnic acid improves memory impairment after cerebral ischemia/ reperfusion injuries by anti-neuroinflammatory, anti-oxidant, and anti-apoptotic properties [J]. Iran J Basic Med Sci , 2020, 23(9): 1225-1231.Biogerontology , 2019, 20(3): 279-301.et al . Role of HMGB1 translocation to neuronal nucleus in rat model with septic brain injury [J]. Neurosci Lett , 2017, 645: 90-96.et al . Dexmedetomidine post-conditioning alleviates cerebral ischemia-reperfusion injury in rats by inhibiting high mobility group protein B1 group (HMGB1)/Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) signaling pathway [J]. Med Sci Monit , 2020, 26: e918617.et al . Neuronal-targeted TFEB rescues dysfunction of the autophagy-lysosomal pathway and alleviates ischemic injury in permanent cerebral ischemia [J]. Autophagy , 2019, 15(3): 493-509.Neurotherapeutics , 2020, 17(4): 1757-1767.et al . Potential therapeutic targets for intracerebral hemorrhage-associated inflammation: An update [J]. J Cereb Blood Flow Metab , 2020, 40(9): 1752-1768.et al . Usnic acid enantiomers restore cognitive deficits and neurochemical alterations induced by Aβ1-42 in mice [J]. Behav Brain Res , 2021, 397: 112945.et al . Cerebral hemorrhage: Pathophysiology, treatment, and future directions [J]. Circ Res , 2022, 130(8): 1204-1229.et al . TREM2 activation attenuates neuroinflammation and neuronal apoptosis via PI3K/Akt pathway after intracerebral hemorrhage in mice [J]. J Neuroinflammation , 2020, 17(1): 168.et al . Melatonin alleviates intracerebral hemorrhage-induced secondary brain injury in rats via suppressing apoptosis, inflammation, oxidative stress, DNA damage, and mitochondria injury [J]. Transl Stroke Res , 2018, 9(1): 74-91.et al . Oxymatrine attenuates sepsis-induced inflammation and organ injury via inhibition of HMGB1/RAGE/NF-κB signaling pathway [J]. Drug Dev Res , 2024, 85(4): e22219.et al . Inhibiting HMGB1-RAGE axis prevents pro-inflammatory macrophages/microglia polarization and affords neuroprotection after spinal cord injury [J]. J Neuroinflammation , 2020, 17(1): 295.
中草药
|药理与临床
2026
, 57
(13) :
5140
-5147
基于HMGB1/RAGE通路探究松萝酸对脑出血小鼠炎症损伤的影响及作用机制
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吴俊晓1 , 李艳2 , 史博3 , 陈红4 , 胡成旺3
作者信息
1 南阳医学高等专科学校 康复医学系, 河南 南阳 473061;
通讯作者:
胡成旺
作者简介:
吴俊晓: 吴俊晓（1973—），女，副教授，研究方向为康复医学。Tel:(0377)63526359 E-mail:xeh393@163.com
Effect and mechanism of usnic acid on inflammatory injury in mice with cerebral hemorrhage based on HMGB1/RAGE pathway
WU Junxiao, LI Yan, SHI Bo, CHEN Hong, HU Chengwang
Affiliations
doi: 10.7501/j.issn.0253-2670.2026.13.017
文章导航
目的 基于高迁移率族蛋白B1（high mobility group box 1，HMGB1）/晚期糖基化终末产物受体（receptor for advanced glycation end products，RAGE）通路探究松萝酸对脑出血（intracerebral hemorrhage，ICH）小鼠炎症损伤的影响。方法 将C57BL/6小鼠随机分为假手术组、模型组、HMGB1抑制剂丙酮酸乙酯（40 mg/kg）组及松萝酸低、高剂量（20、30 mg/kg）组和松萝酸（30 mg/kg）＋rHMGB1（8 μg/kg）组，每组18只。采用颅内注射0.5μL胶原酶构建ICH模型，连续3 d给药后，评价各组小鼠改良神经功能缺损评分（modified neurological severity score，mNSS）和左转率；测定小鼠脑组织含水率；ELISA检测脑组织白细胞介素-6（interleukin-6，IL-6）、IL-1β和IL-18水平；苏木素-伊红（hematoxylin-eosin，HE）染色观察脑组织病理改变；免疫荧光观察脑组织小胶质细胞活化情况；TUNEL染色检测脑组织细胞凋亡；Western blotting检测脑组织HMGB1/RAGE通路相关蛋白表达。结果 与假手术组比较，模型组小鼠纹状体组织损伤严重，mNSS、左侧大脑半球含水率、离子化钙结合适配分子1-（ionized calcium-binding adapter molecule 1，Iba1）阳性细胞数、炎症因子水平、细胞凋亡率及HMGB1、RAGE、磷酸化核因子-κB p65（phosphorylated nuclear factor-κB p65，p-NF-κB p65）/NF-κB p65、肿瘤坏死因子-α（tumor necrosis-α，TNF-α）蛋白表达水平显著升高（P ＜0.05），左转率显著降低（P ＜0.05）；与模型组比较，松萝酸低、高剂量组和丙酮酸乙酯组纹状体组织损伤程度减轻，mNSS、左侧大脑半球含水率、Iba1阳性细胞数、炎症因子水平、细胞凋亡率及HMGB1/RAGE通路相关蛋白表达水平显著降低（P ＜0.05），左转率显著升高（P ＜0.05）；rHMGB1显著逆转了松萝酸对ICH小鼠炎症损伤的抑制作用（P ＜0.05）。结论 松萝酸可能通过抑制HMGB1/RAGE通路减轻ICH小鼠炎症损伤。
松萝酸
/
高迁移率族蛋白B1
/
晚期糖基化终末产物受体
/
脑出血
/
炎症损伤
Objective To explore the effect of usnic acid on inflammatory injury in mice with intracerebral hemorrhage (ICH) based on high mobility group box 1 (HMGB1)/receptor for advanced glycation end products (RAGE) pathway. Methods C57BL/6 mice were randomly divided into sham group, model group, HMGB1 inhibitor ethyl pyruvate (40 mg/kg) group, usnic acid low-, high-dose (20, 30 mg/kg) groups and usnic acid (30 mg/kg) + rHMGB1 (8 μg/kg) group, with 18 mice in each group. The ICH model was constructed by intracranial injection of 0.5 μL collagenase. After continuous administration for 3 d, the modified neurological severity score (mNSS) and left turn rate of mice in each group were evaluated. The water rate of brain tissue in mice was determined. ELISA was used to measure the levels of interleukin-6 (IL-6), IL-1β and IL-18 in brain tissue. Hematoxylin-eosin (HE) staining was used to observe pathological changes in brain tissue. Immunofluorescence was used to observe microglia activation in brain tissue. TUNEL staining was used to detect cell apoptosis of brain tissue. Western blotting was used to detect the expressions of HMGB1/RAGE pathway related proteins in brain tissue. Results Compared with sham group, the striatum tissue of mice in model group was severely damaged, mNSS, left cerebral hemisphere water rate, ionized calcium-binding adapter molecule 1 (Iba1) positive cells number, inflammatory factors levels, cell apoptosis rate, HMGB1, RAGE, phosphorylated nuclear factor-κB p65 (p-NF-κB p65)/NF-κB p65 and TNF-α protein expression levels were significantly increased (P < 0.05), and the left turn rate was significantly decreased (P < 0.05). Compared with model group, the damage degree of striatum tissue in usnic acid low-, high-dose group and ethyl pyruvate group was alleviated (P < 0.05), mNSS, left cerebral hemisphere water rate, Iba1 positive cells number, inflammatory factors levels, cell apoptosis rate and HMGB1/RAGE pathway related proteins expression levels were significantly decreased (P < 0.05), and the left turn rate was significantly increased (P < 0.05). rHMGB1 significantly reversed the inhibitory effect of usnic acid on inflammatory injury in ICH mice (P < 0.05). Conclusion Usnic acid may alleviate inflammatory injury in ICH mice by inhibiting HMGB1/RAGE pathway.
usnic acid
/
high mobility group box 1
/
receptor for advanced glycation end product
/
intracerebral hemorrhage
/
inflammatory injury
吴俊晓, 李艳, 史博, 陈红, 胡成旺.
基于HMGB1/RAGE通路探究松萝酸对脑出血小鼠炎症损伤的影响及作用机制.
中草药,
2026
, 57
(13)
: 5140
-5147
.
DOI: 10.7501/j.issn.0253-2670.2026.13.017
WU Junxiao, LI Yan, SHI Bo, CHEN Hong, HU Chengwang.
Effect and mechanism of usnic acid on inflammatory injury in mice with cerebral hemorrhage based on HMGB1/RAGE pathway[J].
Chinese Traditional and Herbal Drugs ,
2026
, 57
(13)
: 5140
-5147
.
DOI: 10.7501/j.issn.0253-2670.2026.13.017
参考文献
引证文献
Sandset E C, Wang X, Carcel C, et al . Sex differences in treatment, radiological features and outcome after intracerebral haemorrhage: Pooled analysis of intensive blood pressure reduction in acute cerebral haemorrhage trials 1 and 2 [J]. Eur Stroke J , 2020, 5(4): 345-350.et al . Effects of Xingnaojing on serum high-sensitivity C-reactive protein and neuron-specific enolase in patients with acute cerebral hemorrhage: A protocol of systematic review and Meta-analysis [J]. Medicine , 2020, 99(45): e21379.JAMA , 2019, 321(13): 1295-1303.J Stroke Cerebrovasc Dis , 2022, 31(9): 106637.et al . Lichen-derived compounds and extracts as biologically active substances with anticancer and neuroprotective properties [J]. Pharmaceuticals , 2021, 14(12): 1293.et al . Usnic acid improves memory impairment after cerebral ischemia/ reperfusion injuries by anti-neuroinflammatory, anti-oxidant, and anti-apoptotic properties [J]. Iran J Basic Med Sci , 2020, 23(9): 1225-1231.Biogerontology , 2019, 20(3): 279-301.et al . Role of HMGB1 translocation to neuronal nucleus in rat model with septic brain injury [J]. Neurosci Lett , 2017, 645: 90-96.et al . Dexmedetomidine post-conditioning alleviates cerebral ischemia-reperfusion injury in rats by inhibiting high mobility group protein B1 group (HMGB1)/Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) signaling pathway [J]. Med Sci Monit , 2020, 26: e918617.et al . Neuronal-targeted TFEB rescues dysfunction of the autophagy-lysosomal pathway and alleviates ischemic injury in permanent cerebral ischemia [J]. Autophagy , 2019, 15(3): 493-509.Neurotherapeutics , 2020, 17(4): 1757-1767.et al . Potential therapeutic targets for intracerebral hemorrhage-associated inflammation: An update [J]. J Cereb Blood Flow Metab , 2020, 40(9): 1752-1768.et al . Usnic acid enantiomers restore cognitive deficits and neurochemical alterations induced by Aβ1-42 in mice [J]. Behav Brain Res , 2021, 397: 112945.et al . Cerebral hemorrhage: Pathophysiology, treatment, and future directions [J]. Circ Res , 2022, 130(8): 1204-1229.et al . TREM2 activation attenuates neuroinflammation and neuronal apoptosis via PI3K/Akt pathway after intracerebral hemorrhage in mice [J]. J Neuroinflammation , 2020, 17(1): 168.et al . Melatonin alleviates intracerebral hemorrhage-induced secondary brain injury in rats via suppressing apoptosis, inflammation, oxidative stress, DNA damage, and mitochondria injury [J]. Transl Stroke Res , 2018, 9(1): 74-91.et al . Oxymatrine attenuates sepsis-induced inflammation and organ injury via inhibition of HMGB1/RAGE/NF-κB signaling pathway [J]. Drug Dev Res , 2024, 85(4): e22219.et al . Inhibiting HMGB1-RAGE axis prevents pro-inflammatory macrophages/microglia polarization and affords neuroprotection after spinal cord injury [J]. J Neuroinflammation , 2020, 17(1): 295.
2026年第57卷第13期
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doi: 10.7501/j.issn.0253-2670.2026.13.017
接收时间：2025-12-08
首发时间：2026-09-09
https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.13.017
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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