Article(id=1304388233270420186, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388135723496407, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.13.005, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1764864000000, receivedDateStr=2025-12-05, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788919989173, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788919989173, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788919989173, creator=13701087609, updateTime=1788919989173, updator=13701087609, issue=Issue{id=1304388135723496407, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='13', pageStart='4949', pageEnd='5352', issueExtLink='null', onlineDate='null', pubDate='1783785600000', pubDateStr='2026-07-12', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788919965916, creator='13701087609', updateTime=1788923489765, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304402915871977875, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388135723496407, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304402915871977876, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388135723496407, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=4987, endPage=5001, ext={EN=ArticleExt(id=1304388233555632860, articleId=1304388233270420186, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Identifying chemical constituents of Danggui Buxue Decoction and its tissue distribution in mice by UHPLC-Q-Exactive Orbitrap HRMS, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=This study aimed to characterize the chemical profile of Danggui Buxue Decoction (DBD) and investigate the systemic exposure and tissue distribution of its constituents in mice following oral administration, using UHPLC-Q-Exactive Orbitrap high-resolution mass spectrometry (HRMS). Methods A total of 12 healthy male C57BL/6J mice were randomly divided into a blank control group (n = 3), administered purified water, and a DBD-treated group (n = 9), given a DBD extract at 16 g/kg by gavage. Serum and tissue samples were collected at 0.5, 1.5, and 3 h post-dose, pooled, and processed via protein precipitation. Chromatographic separation was performed on an ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) using a gradient elution with 0.1% formic acid in water and methanol as the mobile phase. Mass spectrometric data were acquired in both positive and negative ion modes via full-scan MS1 /data-dependent MS2 scanning. Compound identification was achieved by analyzing relative retention times and MS1 fragment information using Xcalibur 4.2 software, with confirmation against reference standards, literature data, and databases. Results A total of 158 chemical compounds were identified in DBD, comprising 56 flavonoids, 44 saponins, 24 phthalides, 17 organic acids, 8 coumarins, 2 alkaloids, and 7 other constituents. The number of prototype compounds detected in mice after DBD administration varied over time. The abundance of these migrating components across tissues followed the order: ileum > liver > serum > lung > kidney > spleen > heart > brain. Key constituents identified in the serum and various tissues included 7,3' -dihydroxy-5' -methoxyisoflavone (pterocarpan E), calycosin-7-O -β-D -glucoside, methylnissolin, calycosin, oroxylin A, isomucronulatol, 3-butylidenephthalide, (6αR ,11αR )-3,9-dimethoxy-10-hydroxypterocarpan and formononetin. Conclusion This study delineates the comprehensive chemical composition of DBD and in vivo absorption and tissue distribution patterns using HRMS, providing crucial insights for identifying the bioactive constituents of DBD and supporting further tissue pharmacokinetic investigations., authors=LI Rongsheng, LIU Hongmei, GUO Yunhe, FU Guosheng, YI Li, XU Ying, LIU Wei, YUAN Weian, ZHANG Linzhang, authorsList=LI Rongsheng, LIU Hongmei, GUO Yunhe, FU Guosheng, YI Li, XU Ying, LIU Wei, YUAN Weian, ZHANG Linzhang, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304388233480135387, articleId=1304388233270420186, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=UHPLC-Q-Exactive Orbitrap HRMS鉴定当归补血汤的化学成分及小鼠体内成分分布, columnId=1304140187169214944, journalTitle=中草药, columnName=化学成分, runingTitle=null, highlight=null, articleAbstract=目的 采用UHPLC-Q-Exactive Orbitrap HRMS技术鉴定当归补血汤的化学成分，并分析其在小鼠ig后的入血及组织移行分布特征。方法 选取12只健康雄性C57BL/6J小鼠，随机分为空白组（n ＝3）ig纯化水，给药组（n ＝9）按16 g/kg ig当归补血汤提取液，给药后0.5、1.5、3 h采集血清及组织样本，合并后蛋白沉淀法处理。采用ACQUITY UPLC BEH C₁₈色谱柱（100 mm×2.1 mm，1.7 μm），以0.1%甲酸水溶液-甲醇为流动相梯度洗脱分离。质谱数据通过正负离子一级全扫描/数据依赖二级扫描模式采集，结合Xcalibur 4.2软件分析离子峰相对保留时间及一级碎片信息，并通过对照品、文献及数据库比对实现成分鉴定。结果 从当归补血汤中共鉴定出158个化学成分，包括黄酮类56个、皂苷类44个、苯酞类24个、有机酸类17个、香豆素类8个、生物碱类2个以及其他类7个。小鼠ig当归补血汤后原型移行成分数量随时间变化，分布规律为回肠＞肝＞血清＞肺＞肾＞脾＞心＞脑。在血清和各组织中移行成分包括7,3'-二羟基-5'-甲氧基异黄酮（紫檀素E）、毛蕊异黄酮葡萄糖苷、美迪紫檀素、毛蕊异黄酮、千层纸素A、异短尖剑豆酚、丁烯基苯酞、(6αR ,11αR )-3,9-二甲氧基-10-羟基紫檀烷以及芒柄花素。结论 通过高分辨质谱技术解析了当归补血汤化学物质组成及其体内移行特征，为当归补血汤的药效物质解析及组织药代动力研究提供了重要参考。, authors=李荣胜1,2 , 刘红梅3 , 郭云鹤4 , 付国胜2 , 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(2018-04-13) [2026-06-24]. https://www.gov.cn/zhengce/zhengceku/ 2018-12/31/content_5429153. htm.et al . Danggui Buxue Decoction attenuates doxorubicin-induced cardiotoxicity by inhibiting ZBP1-mediated PANoptosis in vivo and in vitro [J]. Phytomedicine , 2025, 145: 157037.et al . Analysis of the mechanism underlying diabetic wound healing acceleration by calycosin-7-glycoside using network pharmacology and molecular docking [J]. Phytomedicine , 2023, 114: 154773.
中草药
|化学成分
2026
, 57
(13) :
4987
-5001
UHPLC-Q-Exactive Orbitrap HRMS鉴定当归补血汤的化学成分及小鼠体内成分分布
全屏
李荣胜1,2 , 刘红梅3 , 郭云鹤4 , 付国胜2 , 仪丽5 , 徐莹6 , 刘伟2 , 元唯安1 , 张霖璋1,2
作者信息
1 上海中医药大学附属曙光医院, 临床研究中心, 上海 201203;
通讯作者:
元唯安
作者简介:
李荣胜: 李荣胜，高级工程师，研究方向为药物临床试验管理。E-mail:lys006@qq.com
刘红梅: 刘红梅，副主任医师，从事中药及复方干预脑病研究。E-mail:1951547770@qq.com
Identifying chemical constituents of Danggui Buxue Decoction and its tissue distribution in mice by UHPLC-Q-Exactive Orbitrap HRMS
LI Rongsheng, LIU Hongmei, GUO Yunhe, FU Guosheng, YI Li, XU Ying, LIU Wei, YUAN Weian, ZHANG Linzhang
Affiliations
doi: 10.7501/j.issn.0253-2670.2026.13.005
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目的 采用UHPLC-Q-Exactive Orbitrap HRMS技术鉴定当归补血汤的化学成分，并分析其在小鼠ig后的入血及组织移行分布特征。方法 选取12只健康雄性C57BL/6J小鼠，随机分为空白组（n ＝3）ig纯化水，给药组（n ＝9）按16 g/kg ig当归补血汤提取液，给药后0.5、1.5、3 h采集血清及组织样本，合并后蛋白沉淀法处理。采用ACQUITY UPLC BEH C₁₈色谱柱（100 mm×2.1 mm，1.7 μm），以0.1%甲酸水溶液-甲醇为流动相梯度洗脱分离。质谱数据通过正负离子一级全扫描/数据依赖二级扫描模式采集，结合Xcalibur 4.2软件分析离子峰相对保留时间及一级碎片信息，并通过对照品、文献及数据库比对实现成分鉴定。结果 从当归补血汤中共鉴定出158个化学成分，包括黄酮类56个、皂苷类44个、苯酞类24个、有机酸类17个、香豆素类8个、生物碱类2个以及其他类7个。小鼠ig当归补血汤后原型移行成分数量随时间变化，分布规律为回肠＞肝＞血清＞肺＞肾＞脾＞心＞脑。在血清和各组织中移行成分包括7,3'-二羟基-5'-甲氧基异黄酮（紫檀素E）、毛蕊异黄酮葡萄糖苷、美迪紫檀素、毛蕊异黄酮、千层纸素A、异短尖剑豆酚、丁烯基苯酞、(6αR ,11αR )-3,9-二甲氧基-10-羟基紫檀烷以及芒柄花素。结论 通过高分辨质谱技术解析了当归补血汤化学物质组成及其体内移行特征，为当归补血汤的药效物质解析及组织药代动力研究提供了重要参考。
UHPLC-Q-Exactive Orbitrap HRMS
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经典名方
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当归补血汤
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入血成分
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组织分布
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紫檀素E
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毛蕊异黄酮葡萄糖苷
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千层纸素A
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丁烯基苯酞
This study aimed to characterize the chemical profile of Danggui Buxue Decoction (DBD) and investigate the systemic exposure and tissue distribution of its constituents in mice following oral administration, using UHPLC-Q-Exactive Orbitrap high-resolution mass spectrometry (HRMS). Methods A total of 12 healthy male C57BL/6J mice were randomly divided into a blank control group (n = 3), administered purified water, and a DBD-treated group (n = 9), given a DBD extract at 16 g/kg by gavage. Serum and tissue samples were collected at 0.5, 1.5, and 3 h post-dose, pooled, and processed via protein precipitation. Chromatographic separation was performed on an ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) using a gradient elution with 0.1% formic acid in water and methanol as the mobile phase. Mass spectrometric data were acquired in both positive and negative ion modes via full-scan MS1 /data-dependent MS2 scanning. Compound identification was achieved by analyzing relative retention times and MS1 fragment information using Xcalibur 4.2 software, with confirmation against reference standards, literature data, and databases. Results A total of 158 chemical compounds were identified in DBD, comprising 56 flavonoids, 44 saponins, 24 phthalides, 17 organic acids, 8 coumarins, 2 alkaloids, and 7 other constituents. The number of prototype compounds detected in mice after DBD administration varied over time. The abundance of these migrating components across tissues followed the order: ileum > liver > serum > lung > kidney > spleen > heart > brain. Key constituents identified in the serum and various tissues included 7,3' -dihydroxy-5' -methoxyisoflavone (pterocarpan E), calycosin-7-O -β-D -glucoside, methylnissolin, calycosin, oroxylin A, isomucronulatol, 3-butylidenephthalide, (6αR ,11αR )-3,9-dimethoxy-10-hydroxypterocarpan and formononetin. Conclusion This study delineates the comprehensive chemical composition of DBD and in vivo absorption and tissue distribution patterns using HRMS, providing crucial insights for identifying the bioactive constituents of DBD and supporting further tissue pharmacokinetic investigations.
UHPLC-Q-Exactive Orbitrap HRMS
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famous classical formulas
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Danggui Buxue Decoction
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constituents absorbed into serum
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tissue distribution
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7,3' -dihydroxy-5' -methoxyisoflavone
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calycosin-7-O -β -D -glucoside
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oroxylin A
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3-butylidenephthalide
李荣胜, 刘红梅, 郭云鹤, 付国胜, 仪丽, 徐莹, 刘伟, 元唯安, 张霖璋.
UHPLC-Q-Exactive Orbitrap HRMS鉴定当归补血汤的化学成分及小鼠体内成分分布.
中草药,
2026
, 57
(13)
: 4987
-5001
.
DOI: 10.7501/j.issn.0253-2670.2026.13.005
LI Rongsheng, LIU Hongmei, GUO Yunhe, FU Guosheng, YI Li, XU Ying, LIU Wei, YUAN Weian, ZHANG Linzhang.
Identifying chemical constituents of Danggui Buxue Decoction and its tissue distribution in mice by UHPLC-Q-Exactive Orbitrap HRMS[J].
Chinese Traditional and Herbal Drugs ,
2026
, 57
(13)
: 4987
-5001
.
DOI: 10.7501/j.issn.0253-2670.2026.13.005
国家自然科学基金项目 (82505535); 国家自然科学基金项目 (82575089); 国家自然科学基金项目 (82474045); 中国博士后基金面上项目 (2025M773981); 国家资助博士后研究人员计划C档资助 (GZC20252641); 上海市自然基金项目 (25ZR1401335,24ZR1467100,25ZR1402483); 上海市进一步加快中医药传承创新发展三年行动计划资助[ZY (2025-2027); 国家中医药管理局中药创新能力提升项目 (BJTH 2024-02F-0009); 上海市卫健委医学新技术研究与转化种子计划项目 (2024ZZ1006); 上海市科委科技启明星项目 (24QA2709200); 上海市东方英才计划青年项目 (QNWS2024054); 上海市超级博士后资助项目 (2024530); 上海市卫健委中医药科研项目计划 (2024QN084)
参考文献
引证文献
国家中医药管理局. 国家中医药管理局关于发布《古代经典名方目录（第一批）》的通知 [EB/OL]. (2018-04-13) [2026-06-24]. https://www.gov.cn/zhengce/zhengceku/ 2018-12/31/content_5429153. htm.et al . Danggui Buxue Decoction attenuates doxorubicin-induced cardiotoxicity by inhibiting ZBP1-mediated PANoptosis in vivo and in vitro [J]. Phytomedicine , 2025, 145: 157037.et al . Analysis of the mechanism underlying diabetic wound healing acceleration by calycosin-7-glycoside using network pharmacology and molecular docking [J]. Phytomedicine , 2023, 114: 154773.
2026年第57卷第13期
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doi: 10.7501/j.issn.0253-2670.2026.13.005
接收时间：2025-12-05
首发时间：2026-09-09
https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.13.005
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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