Article(id=1304388140827964406, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388049975137100, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.11.022, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1763568000000, receivedDateStr=2025-11-20, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788919967133, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788919967133, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788919967133, creator=13701087609, updateTime=1788919967133, updator=13701087609, issue=Issue{id=1304388049975137100, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='11', pageStart='4089', pageEnd='4508', issueExtLink='null', onlineDate='null', pubDate='1781193600000', pubDateStr='2026-06-12', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788919945471, creator='13701087609', updateTime=1788923432386, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304402675202805770, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388049975137100, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304402675207000075, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388049975137100, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=4351, endPage=4363, ext={EN=ArticleExt(id=1304388142988030968, articleId=1304388140827964406, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Revealing regulatory mechanism of low-temperature on accumulation of active components in Veronica polita based on transcriptome and metabolome, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective To investigate the regulatory mechanism underlying the effect of low-temperature treatment on the accumulation of active components in Veronica polita and clarify its regulatory mechanism. Methods Plants subjected to low-temperature treatment were analyzed through phenotypic observation, physiological and biochemical indices, transcriptome and metabolome analyses. Results Prolonged low-temperature treatment significantly increased the plant height, fresh weight and dry weight, as well as the root length of V. polita. The activities of superoxide dismutase (SOD) and peroxidase (POD), as well as the contents of total flavonoids, total phenols and total sugars first rose and then decreased. The contents of malondialdehyde (MDA) and chlorophyll increased, whereas chlorophyll fluorescence parameters decreased. The contents of total flavonoids and total phenols also first increased and then decreased. Transcriptomic analysis showed that the differentially expressed genes (DEGs) exhibited a stage-specific expression patterns. Pathways such as secondary metabolite biosynthesis, phenylpropanoid biosynthesis, metabolic pathways, and plant hormone signal transduction were significantly enriched. Three flavonoid synthesis-related genes (GT5, JOX2, HO1) and five phenol synthesis and metabolism-related genes (LAC3, AOX4, LPR2, AS1, MT-CO2) were identified. Metabolomic analysis indicated that the contents of quercetin, myricetin, and benzoic acid showed a trend of first increasing and then decreasing with the extension of treatment time. Integrated multi-omics analysis demonstrated that the core mechanism by which V. polita responds to low-temperature stress involves the precise reprogramming of the phenylpropanoid/flavonoid metabolic pathway. Conclusion Appropriatelow-temperature treatment promotes the accumulation of secondary metabolites in V. polita, thereby providing a foundation for further research into its functional genes., authors=JIN Minrui, SU Yanlong, ZHOU Xiangyan, LI Mengfei, authorsList=JIN Minrui, SU Yanlong, ZHOU Xiangyan, LI Mengfei, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304388142895756279, articleId=1304388140827964406, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=转录组学联合代谢组学揭示低温对婆婆纳活性成分积累的调控机制, columnId=1304140203967410414, journalTitle=中草药, columnName=药材与资源, runingTitle=null, highlight=null, articleAbstract=目的 研究低温处理对婆婆纳Veronica polita活性成分积累的影响，并阐明其调控机制。方法 对经低温处理的婆婆纳进行表型观察、生理生化指标测定、转录组及代谢组分析。结果 随着低温处理时间的延长，婆婆纳的株高、鲜质量、干质量，以及根长均显著增加。超氧化物歧化酶（superoxide dismutase，SOD）和过氧化物酶（peroxidase，POD）活性、总黄酮、总酚及总糖含量均先升高后降低，丙二醛（malondialdehyde，MDA）、叶绿素含量及过氧化氢酶（catalase，CAT）活性增加，叶绿素荧光参数降低。转录组分析显示，差异表达基因（differentially expressed genes，DEGs）呈阶段特异性表达模式：次生代谢物生物合成、苯丙烷类生物合成、代谢途径、植物激素信号转导等通路DEGs显著富集：筛选出3个黄酮类合成相关基因（GT5、JOX2、HO1）和5个酚类物质合成与代谢相关基因（LAC3、AOX4、LPR2、AS1、MT-CO2）。代谢物中槲皮素、杨梅素和苯甲酸含量随着处理时间延长，呈先升高后降低的趋势。整合多组学分析揭示，婆婆纳响应低温的机制主要为苯丙烷/类黄酮代谢通路的调控。结论 适度低温处理有助于婆婆纳次生代谢物的积累，为其功能基因的深入研究奠定了基础。, authors=金敏睿1, 苏衍龙2, 周香艳1, 栗孟飞1,2, authorsList=金敏睿, 苏衍龙, 周香艳, 栗孟飞, authorCompany=1 甘肃农业大学生命科学技术学院, 甘肃 兰州 730070; 2 甘肃农业大学农学院 干旱生境作物学国家重点实验室, 甘肃 兰州 730070, correspAuthors=周香艳, authorNote=金敏睿: 金敏睿（2000—），男，甘肃兰州人，硕士研究生，主要从事生物技术育种方面研究。Tel:13893603207 E-mail:1426257490@qq.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, 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Physiological mechanisms of resistance to cold stress associated with 10 elite apple rootstocks [J]. J Integr Agric, 2018, 17(4): 857-866. 刘天乐, 朱田田, 晋玲, 等. 当归AP2/ERF转录因子家族鉴定及干旱胁迫下的表达分析[J]. 中草药, 2024, 55(14): 4849-4859. 陈佳茜, 郭广洋, 谭新杰, 等. 连翘多胺氧化酶基因家族的鉴定及干旱和盐胁迫下的表达分析[J]. 中草药, 2024, 55(9): 3077-3084. Manasa S L, Panigrahy M, Panigrahi K C S, et al. Overview of cold stress regulation in plants [J]. Bot Rev, 2022, 88(3): 359-387. Ding Y L, Shi Y T, Yang S H. Advances and challenges in uncovering cold tolerance regulatory mechanisms in plants [J]. New Phytol, 2019, 222(4): 1690-1704. Székely Á, Szalóki T, Lantos C, et al. Data of selected set of rice accessions at the germination stage under cold stress [J]. Data Brief, 2022, 41: 107929. 李佳荫, 冯国军, 刘大军, 等. 低温胁迫对菜豆种子吸胀期间生理特性和发芽能力的影响[J]. 中国农学通报, 2020, 36(1): 24-29. 连艺佳, 刘瑞, 王嘉杰, 等. 低温对冬小麦萌发生理特性的影响[J]. 北京农学院学报, 2023, 38(4): 1-7. 刘洋. 基于高通量测序分析灌浆初期低温对小麦颖果淀粉积累的影响[D]. 扬州: 扬州大学, 2023. Zhang T, Chen C B, Chen Y Q, et al. Changes in the leaf physiological characteristics and tissue-specific distribution of ginsenosides in Panax ginseng during flowering stage under cold stress [J]. Front Bioeng Biotechnol, 2021, 9: 637324. 马文琪, 王红阳,张文晋, 等. 生态因子对人参外形和皂苷类成分的影响2021, 46(8): 1920-1926. Deng D Q, Fan J, Cao L, et al. Effect of low-temperature stress on secondary metabolism of Astragalus membranaceus Bge. var. mongolicus Hsiao [J]. Russ J Plant Physiol, 2024, 71(4): 105. Abla M, Sun H G, Li Z Y, et al. Identification of miRNAs and their response to cold stress in Astragalus membranaceus [J]. Biomolecules, 2019, 9(5): 182. Daniell H, Lin C S, Yu M, et al. Chloroplast genomes: Diversity, evolution, and applications in genetic engineering [J]. Genome Biol, 2016, 17(1): 134. Wicke S, Schneeweiss G M, DePamphilis C W, et al. The evolution of the plastid chromosome in land plants: Gene content, gene order, gene function [J]. Plant Mol Biol, 2011, 76(3): 273-297. Egamberdiev S S, Saha S, Salakhutdinov I, et al. Comparative assessment of genetic diversity in cytoplasmic and nuclear genome of upland cotton [J]. Genetica, 2016, 144(3): 289-306. Liu H X, Wang Y, Xu J, et al. Ethylene signaling is required for the acceleration of cell death induced by the activation of AtMEK5 in Arabidopsis [J]. Cell Res, 2008, 18(3): 422-432. 冯汉青, 赵玲, 庞海龙, 等. 低温胁迫下交替呼吸途径对小麦幼根生长及氧化压力的调节作用[J]. 西北师范大学学报: 自然科学版, 2020, 56(4): 78-83. 旦增曲培, 郭肖, 东知多杰, 等. 藏药巴夏嘎本草考证及同名异物形成原因分析[J]. 世界科学技术—中医药现代化, 2020, 22(10): 3746-3751. 兰淑芝, 李伯清, 低温对植物的影响[J]. 吉林农业, 1997(8): 19-20. Król A, Amarowicz R, Weidner S. The effects of cold stress on the phenolic compounds and antioxidant capacity of grapevine (Vitis vinifera L.) leaves [J]. J Plant Physiol, 2015, 189: 97-104. Hajihashemi S, Noedoost F, Geuns J M C, et al. Effect of cold stress on photosynthetic traits, carbohydrates, morphology, and anatomy in nine cultivars of Stevia rebaudiana [J]. Front Plant Sci, 2018, 9: 1430. 晏方勤, 李晓, 焦坤, 等. 邻作婆婆纳对花生苗期主要害虫和天敌种群的影响[J]. 花生学报, 2022, 51(1): 23-29. 李庚飞, 徐承. 婆婆纳对铅污染耐受性的研究[J]. 北方园艺, 2015(8): 70-73. 赵云山, 毕雅琼, 雷露静, 等. “巴沙嘎”类蒙药品种整理与研究进展[J]. 中国中药杂志, 2017, 42(5): 998-1004. 王恒, 欧阳辉, 唐辉, 等. 婆婆纳的性状与显微鉴定[J]. 中药材, 2017, 40(2): 325-327. 王莉娟, 张樱山, 马瑞瑞, 等. 藏药婆婆纳HPLC特征图谱的建立与主成分含量测定[J]. 中兽医医药杂志, 2022, 41(3): 17-22. Barreira J C M, Dias M I, Živković J, et al. Phenolic profiling of Veronica spp. grown in mountain, urban and sandy soil environments [J]. Food Chem, 2014, 163: 275-283. 许小涵, 唐志强, 刘谦, 等. 干旱胁迫下忍冬全基因组DNA甲基化和转录组分析[J]. 中草药, 2023, 54(16): 5339-5349. Price J C, Udall J A, Bodily P M, et al. De novo identification of ‘heterotigs’ towards accurate and in-phase assembly of complex plant genomes [J]. Internat Conferen Bioinformat Computat Biol, 2012, 52: 26. Tillich M, Lehwark P, Pellizzer T, et al. GeSeq-versatile and accurate annotation of organelle genomes [J]. Nucleic Acids Res, 2017, 45(W1): W6-W11. Zheng S Y, Poczai P, Hyvönen J, et al. Chloroplot: An online program for the versatile plotting of organelle genomes [J]. Front Genet, 2020, 11: 576124. 孙晓琛, 栗锦鹏, 原静静, 等. 基于转录组测序分析干旱胁迫对党参不同组织基因表达的调控[J]. 中草药, 2022, 53(14): 4465-4475. Amiryousefi A, Hyvönen J, Poczai P. IRscope: An online program to visualize the junction sites of chloroplast genomes [J]. Bioinformatics, 2018, 34(17): 3030-3031. Rentel M C, Lecourieux D, Ouaked F, et al. OXI1 kinase is necessary for oxidative burst-mediated signalling in Arabidopsis [J]. Nature, 2004, 427(6977): 858-861. Milne I, Lindner D, Bayer M, et al. TOPALi v2: A rich graphical interface for evolutionary analyses of multiple alignments on HPC clusters and multi-core desktops [J]. Bioinformatics, 2009, 25(1): 126-127. Xu K, Zhao Y, Gu J, et al. Proteomic analysis reveals the molecular mechanism underlying the cold acclimation and freezing tolerance of wheat (Triticum aestivum L.) [J]. Plant Sci, 2022, 318: 111242. John Martin J J, Song Y Q, Hou M M, et al. Multi-omics approaches in oil palm research: A comprehensive review of metabolomics, proteomics, and transcriptomics based on low-temperature stress [J]. Int J Mol Sci, 2024, 25(14): 7695. Junker B H, Lonien J, Heady L E, et al. Parallel determination of enzyme activities and in vivo fluxes in Brassica napus embryos grown on organic or inorganic nitrogen source [J]. Phytochemistry, 2007, 68(16/17/18): 2232-2242. Yang X Y, Zhao T Y, Rao P, et al. Transcriptome profiling of Populus tomentosa under cold stress [J]. Ind Crops Prod, 2019, 135: 283-293. Li Y Y, Wang X W, Ban Q Y, et al. Comparative transcriptomic analysis reveals gene expression associated with cold adaptation in the tea plant Camellia sinensis [J]. BMC Genomics, 2019, 20(1): 624. Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels [J]. Anal Biochem, 1971, 44(1): 276-287. Chance B, Maehly A C. Assay of catalases and peroxidases [M] Amsterdam: Elsevier, 1955: 764-775. Aebi H. Catalase in vitro [M]// Oxygen Radicals in Biological Systems. Amsterdam: Elsevier, 1984, 105: 121-126. Heath R L, Packer L. Reprint of: Photoperoxidation in isolated chloroplasts I. kinetics and stoichiometry of fatty acid peroxidation [J]. Arch Biochem Biophys, 2022, 726: 109248. DuBois M, Gilles K A, Hamilton J K, et al. Colorimetric method for determination of sugars and related substances [J]. Anal Chem, 1956, 28(3): 350-356. Jia Z S, Tang M C, Wu J M. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals [J]. Food Chem, 1999, 64(4): 555-559. Singleton V L, Rossi J A Jr. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents [J]. Am J Enol Vitic, 1965, 16(3): 144-158. Yun Z, Jin S, Ding Y D, et al. Comparative transcriptomics and proteomics analysis of Citrus fruit, to improve understanding of the effect of low temperature on maintaining fruit quality during lengthy post-harvest storage [J]. J Exp Bot, 2012, 63(8): 2873-2893. Xu G X, Li L J, Zhou J, et al. Comparison of transcriptome and metabolome analysis revealed differences in cold resistant metabolic pathways in different apple cultivars under low temperature stress [J]. Hortic Plant J, 2023, 9(2): 183-198. Shahan R, Nolan T M, Benfey P N. Single-cell analysis of cell identity in the Arabidopsis root apical meristem: Insights and opportunities [J]. J Exp Bot, 2021, 72(19): 6679-6686. Didovyk A, Borek B, Tsimring L, et al. Transcriptional regulation with CRISPR-Cas9: Principles, advances, and applications [J]. Curr Opin Biotechnol, 2016, 40: 177-184. Guo Q Q, Li X, Niu L, et al. Transcription-associated metabolomic adjustments in maize occur during combined drought and cold stress [J]. Plant Physiol, 2021, 186(1): 677-695. Li Q, Byrns B, Badawi M A, et al. Transcriptomic insights into phenological development and cold tolerance of wheat grown in the field [J]. Plant Physiol, 2018, 176(3): 2376-2394. 孙国庆, 刘建雨, 吴发明, 等. 逆境胁迫下红花CYP450s基因的表达与幼叶总黄酮含量的相关性分析[J]. 中草药, 2022, 53(1): 222-230. Wang P F, Dai L M, Ai J, et al. Identification and functional prediction of cold-related long non-coding RNA (lncRNA) in grapevine [J]. Sci Rep, 2019, 9(1): 6638. Svensson J T, Crosatti C, Campoli C, et al. Transcriptome analysis of cold acclimation in barley Albina and xantha mutants [J]. Plant Physiol, 2006, 141(1): 257-270.)
Objective To investigate the regulatory mechanism underlying the effect of low-temperature treatment on the accumulation of active components in Veronica polita and clarify its regulatory mechanism. Methods Plants subjected to low-temperature treatment were analyzed through phenotypic observation, physiological and biochemical indices, transcriptome and metabolome analyses. Results Prolonged low-temperature treatment significantly increased the plant height, fresh weight and dry weight, as well as the root length of V. polita. The activities of superoxide dismutase (SOD) and peroxidase (POD), as well as the contents of total flavonoids, total phenols and total sugars first rose and then decreased. The contents of malondialdehyde (MDA) and chlorophyll increased, whereas chlorophyll fluorescence parameters decreased. The contents of total flavonoids and total phenols also first increased and then decreased. Transcriptomic analysis showed that the differentially expressed genes (DEGs) exhibited a stage-specific expression patterns. Pathways such as secondary metabolite biosynthesis, phenylpropanoid biosynthesis, metabolic pathways, and plant hormone signal transduction were significantly enriched. Three flavonoid synthesis-related genes (GT5, JOX2, HO1) and five phenol synthesis and metabolism-related genes (LAC3, AOX4, LPR2, AS1, MT-CO2) were identified. Metabolomic analysis indicated that the contents of quercetin, myricetin, and benzoic acid showed a trend of first increasing and then decreasing with the extension of treatment time. Integrated multi-omics analysis demonstrated that the core mechanism by which V. polita responds to low-temperature stress involves the precise reprogramming of the phenylpropanoid/flavonoid metabolic pathway. Conclusion Appropriatelow-temperature treatment promotes the accumulation of secondary metabolites in V. polita, thereby providing a foundation for further research into its functional genes.
JIN Minrui, SU Yanlong, ZHOU Xiangyan, LI Mengfei.
Revealing regulatory mechanism of low-temperature on accumulation of active components in Veronica polita based on transcriptome and metabolome[J].
Chinese Traditional and Herbal Drugs,
2026
, 57
(11)
: 4351
-4363
.
DOI: 10.7501/j.issn.0253-2670.2026.11.022
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收起
甘肃省自然科学基金重点项目 (25JRRA349); 国家现代农业产业技术体系 (CARS-21)
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Wang Y X, Hu Y, Chen B H, et al. Physiological mechanisms of resistance to cold stress associated with 10 elite apple rootstocks [J]. J Integr Agric, 2018, 17(4): 857-866. 刘天乐, 朱田田, 晋玲, 等. 当归AP2/ERF转录因子家族鉴定及干旱胁迫下的表达分析[J]. 中草药, 2024, 55(14): 4849-4859. 陈佳茜, 郭广洋, 谭新杰, 等. 连翘多胺氧化酶基因家族的鉴定及干旱和盐胁迫下的表达分析[J]. 中草药, 2024, 55(9): 3077-3084. Manasa S L, Panigrahy M, Panigrahi K C S, et al. Overview of cold stress regulation in plants [J]. Bot Rev, 2022, 88(3): 359-387. Ding Y L, Shi Y T, Yang S H. Advances and challenges in uncovering cold tolerance regulatory mechanisms in plants [J]. New Phytol, 2019, 222(4): 1690-1704. Székely Á, Szalóki T, Lantos C, et al. Data of selected set of rice accessions at the germination stage under cold stress [J]. Data Brief, 2022, 41: 107929. 李佳荫, 冯国军, 刘大军, 等. 低温胁迫对菜豆种子吸胀期间生理特性和发芽能力的影响[J]. 中国农学通报, 2020, 36(1): 24-29. 连艺佳, 刘瑞, 王嘉杰, 等. 低温对冬小麦萌发生理特性的影响[J]. 北京农学院学报, 2023, 38(4): 1-7. 刘洋. 基于高通量测序分析灌浆初期低温对小麦颖果淀粉积累的影响[D]. 扬州: 扬州大学, 2023. Zhang T, Chen C B, Chen Y Q, et al. Changes in the leaf physiological characteristics and tissue-specific distribution of ginsenosides in Panax ginseng during flowering stage under cold stress [J]. Front Bioeng Biotechnol, 2021, 9: 637324. 马文琪, 王红阳,张文晋, 等. 生态因子对人参外形和皂苷类成分的影响2021, 46(8): 1920-1926. Deng D Q, Fan J, Cao L, et al. Effect of low-temperature stress on secondary metabolism of Astragalus membranaceus Bge. var. mongolicus Hsiao [J]. Russ J Plant Physiol, 2024, 71(4): 105. Abla M, Sun H G, Li Z Y, et al. Identification of miRNAs and their response to cold stress in Astragalus membranaceus [J]. Biomolecules, 2019, 9(5): 182. Daniell H, Lin C S, Yu M, et al. Chloroplast genomes: Diversity, evolution, and applications in genetic engineering [J]. Genome Biol, 2016, 17(1): 134. Wicke S, Schneeweiss G M, DePamphilis C W, et al. The evolution of the plastid chromosome in land plants: Gene content, gene order, gene function [J]. Plant Mol Biol, 2011, 76(3): 273-297. Egamberdiev S S, Saha S, Salakhutdinov I, et al. Comparative assessment of genetic diversity in cytoplasmic and nuclear genome of upland cotton [J]. Genetica, 2016, 144(3): 289-306. Liu H X, Wang Y, Xu J, et al. Ethylene signaling is required for the acceleration of cell death induced by the activation of AtMEK5 in Arabidopsis [J]. Cell Res, 2008, 18(3): 422-432. 冯汉青, 赵玲, 庞海龙, 等. 低温胁迫下交替呼吸途径对小麦幼根生长及氧化压力的调节作用[J]. 西北师范大学学报: 自然科学版, 2020, 56(4): 78-83. 旦增曲培, 郭肖, 东知多杰, 等. 藏药巴夏嘎本草考证及同名异物形成原因分析[J]. 世界科学技术—中医药现代化, 2020, 22(10): 3746-3751. 兰淑芝, 李伯清, 低温对植物的影响[J]. 吉林农业, 1997(8): 19-20. Król A, Amarowicz R, Weidner S. The effects of cold stress on the phenolic compounds and antioxidant capacity of grapevine (Vitis vinifera L.) leaves [J]. J Plant Physiol, 2015, 189: 97-104. Hajihashemi S, Noedoost F, Geuns J M C, et al. Effect of cold stress on photosynthetic traits, carbohydrates, morphology, and anatomy in nine cultivars of Stevia rebaudiana [J]. Front Plant Sci, 2018, 9: 1430. 晏方勤, 李晓, 焦坤, 等. 邻作婆婆纳对花生苗期主要害虫和天敌种群的影响[J]. 花生学报, 2022, 51(1): 23-29. 李庚飞, 徐承. 婆婆纳对铅污染耐受性的研究[J]. 北方园艺, 2015(8): 70-73. 赵云山, 毕雅琼, 雷露静, 等. “巴沙嘎”类蒙药品种整理与研究进展[J]. 中国中药杂志, 2017, 42(5): 998-1004. 王恒, 欧阳辉, 唐辉, 等. 婆婆纳的性状与显微鉴定[J]. 中药材, 2017, 40(2): 325-327. 王莉娟, 张樱山, 马瑞瑞, 等. 藏药婆婆纳HPLC特征图谱的建立与主成分含量测定[J]. 中兽医医药杂志, 2022, 41(3): 17-22. Barreira J C M, Dias M I, Živković J, et al. Phenolic profiling of Veronica spp. grown in mountain, urban and sandy soil environments [J]. Food Chem, 2014, 163: 275-283. 许小涵, 唐志强, 刘谦, 等. 干旱胁迫下忍冬全基因组DNA甲基化和转录组分析[J]. 中草药, 2023, 54(16): 5339-5349. Price J C, Udall J A, Bodily P M, et al. De novo identification of ‘heterotigs’ towards accurate and in-phase assembly of complex plant genomes [J]. Internat Conferen Bioinformat Computat Biol, 2012, 52: 26. Tillich M, Lehwark P, Pellizzer T, et al. GeSeq-versatile and accurate annotation of organelle genomes [J]. Nucleic Acids Res, 2017, 45(W1): W6-W11. Zheng S Y, Poczai P, Hyvönen J, et al. Chloroplot: An online program for the versatile plotting of organelle genomes [J]. Front Genet, 2020, 11: 576124. 孙晓琛, 栗锦鹏, 原静静, 等. 基于转录组测序分析干旱胁迫对党参不同组织基因表达的调控[J]. 中草药, 2022, 53(14): 4465-4475. Amiryousefi A, Hyvönen J, Poczai P. IRscope: An online program to visualize the junction sites of chloroplast genomes [J]. Bioinformatics, 2018, 34(17): 3030-3031. Rentel M C, Lecourieux D, Ouaked F, et al. OXI1 kinase is necessary for oxidative burst-mediated signalling in Arabidopsis [J]. Nature, 2004, 427(6977): 858-861. Milne I, Lindner D, Bayer M, et al. TOPALi v2: A rich graphical interface for evolutionary analyses of multiple alignments on HPC clusters and multi-core desktops [J]. Bioinformatics, 2009, 25(1): 126-127. Xu K, Zhao Y, Gu J, et al. Proteomic analysis reveals the molecular mechanism underlying the cold acclimation and freezing tolerance of wheat (Triticum aestivum L.) [J]. Plant Sci, 2022, 318: 111242. John Martin J J, Song Y Q, Hou M M, et al. Multi-omics approaches in oil palm research: A comprehensive review of metabolomics, proteomics, and transcriptomics based on low-temperature stress [J]. Int J Mol Sci, 2024, 25(14): 7695. Junker B H, Lonien J, Heady L E, et al. Parallel determination of enzyme activities and in vivo fluxes in Brassica napus embryos grown on organic or inorganic nitrogen source [J]. Phytochemistry, 2007, 68(16/17/18): 2232-2242. Yang X Y, Zhao T Y, Rao P, et al. Transcriptome profiling of Populus tomentosa under cold stress [J]. Ind Crops Prod, 2019, 135: 283-293. Li Y Y, Wang X W, Ban Q Y, et al. Comparative transcriptomic analysis reveals gene expression associated with cold adaptation in the tea plant Camellia sinensis [J]. BMC Genomics, 2019, 20(1): 624. Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels [J]. Anal Biochem, 1971, 44(1): 276-287. Chance B, Maehly A C. Assay of catalases and peroxidases [M] Amsterdam: Elsevier, 1955: 764-775. Aebi H. Catalase in vitro [M]// Oxygen Radicals in Biological Systems. Amsterdam: Elsevier, 1984, 105: 121-126. Heath R L, Packer L. Reprint of: Photoperoxidation in isolated chloroplasts I. kinetics and stoichiometry of fatty acid peroxidation [J]. Arch Biochem Biophys, 2022, 726: 109248. DuBois M, Gilles K A, Hamilton J K, et al. Colorimetric method for determination of sugars and related substances [J]. Anal Chem, 1956, 28(3): 350-356. Jia Z S, Tang M C, Wu J M. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals [J]. Food Chem, 1999, 64(4): 555-559. Singleton V L, Rossi J A Jr. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents [J]. Am J Enol Vitic, 1965, 16(3): 144-158. Yun Z, Jin S, Ding Y D, et al. Comparative transcriptomics and proteomics analysis of Citrus fruit, to improve understanding of the effect of low temperature on maintaining fruit quality during lengthy post-harvest storage [J]. J Exp Bot, 2012, 63(8): 2873-2893. Xu G X, Li L J, Zhou J, et al. Comparison of transcriptome and metabolome analysis revealed differences in cold resistant metabolic pathways in different apple cultivars under low temperature stress [J]. Hortic Plant J, 2023, 9(2): 183-198. Shahan R, Nolan T M, Benfey P N. Single-cell analysis of cell identity in the Arabidopsis root apical meristem: Insights and opportunities [J]. J Exp Bot, 2021, 72(19): 6679-6686. Didovyk A, Borek B, Tsimring L, et al. Transcriptional regulation with CRISPR-Cas9: Principles, advances, and applications [J]. Curr Opin Biotechnol, 2016, 40: 177-184. Guo Q Q, Li X, Niu L, et al. Transcription-associated metabolomic adjustments in maize occur during combined drought and cold stress [J]. Plant Physiol, 2021, 186(1): 677-695. Li Q, Byrns B, Badawi M A, et al. Transcriptomic insights into phenological development and cold tolerance of wheat grown in the field [J]. Plant Physiol, 2018, 176(3): 2376-2394. 孙国庆, 刘建雨, 吴发明, 等. 逆境胁迫下红花CYP450s基因的表达与幼叶总黄酮含量的相关性分析[J]. 中草药, 2022, 53(1): 222-230. Wang P F, Dai L M, Ai J, et al. Identification and functional prediction of cold-related long non-coding RNA (lncRNA) in grapevine [J]. Sci Rep, 2019, 9(1): 6638. Svensson J T, Crosatti C, Campoli C, et al. Transcriptome analysis of cold acclimation in barley Albina and xantha mutants [J]. Plant Physiol, 2006, 141(1): 257-270.
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