Article(id=1304388091620381674, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388049975137100, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.11.007, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1764000000000, receivedDateStr=2025-11-25, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788919955401, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788919955401, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788919955401, creator=13701087609, updateTime=1788919955401, updator=13701087609, issue=Issue{id=1304388049975137100, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='11', pageStart='4089', pageEnd='4508', issueExtLink='null', onlineDate='null', pubDate='1781193600000', pubDateStr='2026-06-12', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788919945471, creator='13701087609', updateTime=1788923432386, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304402675202805770, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388049975137100, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304402675207000075, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304388049975137100, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=4147, endPage=4157, ext={EN=ArticleExt(id=1304388091989480428, articleId=1304388091620381674, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Analysis on quality attribute of substance benchmarks of Wuwei Xiaodu Yin based on supramolecular “imprinting template” theory, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective Based on the supramolecular “imprinting template” theory, this study investigates the autonomous interaction patterns of the component groups in Wuwei Xiaodu Yin (WXY, 五味消毒饮) during the preparation process from crude herbs to reference samples and elucidates the quantitative transfer law of its quality attributes. Methods Fifteen batches of WXY reference samples were prepared via water decoction. A UPLC fingerprint analysis method was established to identify key chromatographic peaks and determine the contents of chlorogenic acid, esculetin, cichoric acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, glehinnalin, and linarin were determined at 240 nm. Parameters such as transfer rate, extract yield, total statistical moment, and information entropy were calculated for both the herbs and the reference samples. Results The fingerprint similarity of the 15 batches of reference samples was greater than 0.9. Fourteen chromatographic peaks were identified, with 10 characteristic peaks defined. The extract yield ranged from 43.27% to 53.43%. The transfer rates of chlorogenic acid, esculetin, cichoric acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, glehinnalin, and linarin were 34.25%—42.72%, 51.52%—58.80%, 28.21%—32.04%, 56.26%—65.59%, 40.63%—53.89%, 22.35%—31.29%, 38.39%—46.22%, 34.02%—40.82%, 18.64%—20.96%, and 28.55%—32.63%, respectively, with no discrete data observed. The relative standard deviations (RSDs) of the information entropy for the UPLC fingerprints of the five herbs and the reference samples were all less than 10%. The RSDs of mean chromatographic retention time of total quantum (MCRTT ) and information entropy for WXY (crude herbs) were 3.90% and 2.25%, respectively. Conclusion Quality analysis of the reference samples based on the parameters of the supramolecular “imprinting template” provides a scientific basis for the quality control of WXY reference samples and the development of compound formulations., authors=XIAO Qiang, YUAN Qin, ZHANG Zian, CHEN Fan, LIU Danling, HUANG Duanyang, ZENG Hui, PAN Xue, HE Fuyuan, XIAO Meifeng, authorsList=XIAO Qiang, YUAN Qin, ZHANG Zian, CHEN Fan, LIU Danling, HUANG Duanyang, ZENG Hui, PAN Xue, HE Fuyuan, XIAO Meifeng, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304388091872039915, articleId=1304388091620381674, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=基于超分子“印迹模板”理论分析五味消毒饮基准样品的质量属性, columnId=1304140189132149234, journalTitle=中草药, columnName=药剂与工艺, runingTitle=null, highlight=null, articleAbstract=目的 基于超分子“印迹模板”理论，研究五味消毒饮（Wuwei Xiaodu Yin，WXY）成分群在“药材-基准样品”制备过程中的自主作用规律，阐明其质量属性的量值传递规律。方法 水煎法制备15批WXY基准样品，建立UPLC指纹图谱分析方法，指认关键色谱峰，在240 nm波长处测定绿原酸、秦皮乙素、菊苣酸、新绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、格列风内酯和蒙花苷的含量；计算药材及基准样品转移率、出膏率、总量统计矩和信息熵等参数。结果 15批WXY基准样品指纹图谱相似度＞0.9，指认10个色谱峰，设定10个特征色谱峰。15批WXY基准样品出膏率为43.27%～53.43%；绿原酸、秦皮乙素、菊苣酸、新绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、格列风内酯和蒙花苷的转移率依次为34.35%～42.72%、51.52%～58.80%、28.21%～32.04%、56.26%～65.59%、40.63%～53.89%、22.35%～31.29%、38.39%～46.22%、34.02%～40.82%、18.64%～20.96%、28.55%～32.63%，均未出现离散数据；5味药材与基准样品UPLC指纹图谱的信息熵的RSD均＜10%；WXY（药材）的总量一阶矩（mean chromatographic retention time of total quantum，MCRTT）、信息熵的RSD分别为3.90%、2.25%。结论 基于超分子“印迹模板”作用参数的基准样品质量分析，为WXY基准样品的质量控制和复方制剂开发提供科学依据。, authors=肖强1,2 , 袁琴1,2 , 张子桉1,2 , 陈凡1,2 , 刘丹玲1 , 黄端阳1 , 曾慧1 , 潘雪1,2 , 贺福元1,2 , 肖美凤1,2 , authorsList=肖强, 袁琴, 张子桉, 陈凡, 刘丹玲, 黄端阳, 曾慧, 潘雪, 贺福元, 肖美凤, authorCompany=1 湖南中医药大学药学院, 湖南 长沙 410208;
中草药
|药剂与工艺
2026
, 57
(11) :
4147
-4157
基于超分子“印迹模板”理论分析五味消毒饮基准样品的质量属性
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肖强1,2 , 袁琴1,2 , 张子桉1,2 , 陈凡1,2 , 刘丹玲1 , 黄端阳1 , 曾慧1 , 潘雪1,2 , 贺福元1,2 , 肖美凤1,2
作者信息
1 湖南中医药大学药学院, 湖南 长沙 410208;
通讯作者:
贺福元
作者简介:
肖强: 肖强，硕士研究生，研究方向为中药及复方超分子研究。E-mail:2538569297@qq.com
Analysis on quality attribute of substance benchmarks of Wuwei Xiaodu Yin based on supramolecular “imprinting template” theory
XIAO Qiang, YUAN Qin, ZHANG Zian, CHEN Fan, LIU Danling, HUANG Duanyang, ZENG Hui, PAN Xue, HE Fuyuan, XIAO Meifeng
Affiliations
doi: 10.7501/j.issn.0253-2670.2026.11.007
文章导航
目的 基于超分子“印迹模板”理论，研究五味消毒饮（Wuwei Xiaodu Yin，WXY）成分群在“药材-基准样品”制备过程中的自主作用规律，阐明其质量属性的量值传递规律。方法 水煎法制备15批WXY基准样品，建立UPLC指纹图谱分析方法，指认关键色谱峰，在240 nm波长处测定绿原酸、秦皮乙素、菊苣酸、新绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、格列风内酯和蒙花苷的含量；计算药材及基准样品转移率、出膏率、总量统计矩和信息熵等参数。结果 15批WXY基准样品指纹图谱相似度＞0.9，指认10个色谱峰，设定10个特征色谱峰。15批WXY基准样品出膏率为43.27%～53.43%；绿原酸、秦皮乙素、菊苣酸、新绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、格列风内酯和蒙花苷的转移率依次为34.35%～42.72%、51.52%～58.80%、28.21%～32.04%、56.26%～65.59%、40.63%～53.89%、22.35%～31.29%、38.39%～46.22%、34.02%～40.82%、18.64%～20.96%、28.55%～32.63%，均未出现离散数据；5味药材与基准样品UPLC指纹图谱的信息熵的RSD均＜10%；WXY（药材）的总量一阶矩（mean chromatographic retention time of total quantum，MCRTT）、信息熵的RSD分别为3.90%、2.25%。结论 基于超分子“印迹模板”作用参数的基准样品质量分析，为WXY基准样品的质量控制和复方制剂开发提供科学依据。
经典名方
/
五味消毒饮
/
基准样品
/
量值传递规律
/
超分子
/
印迹模板
/
质量属性
/
指纹图谱
/
绿原酸
/
秦皮乙素
/
菊苣酸
/
新绿原酸
/
隐绿原酸
/
异绿原酸A
/
异绿原酸B
/
异绿原酸C
/
格列风内酯
/
蒙花苷
/
总量统计矩
/
信息熵
Objective Based on the supramolecular “imprinting template” theory, this study investigates the autonomous interaction patterns of the component groups in Wuwei Xiaodu Yin (WXY, 五味消毒饮) during the preparation process from crude herbs to reference samples and elucidates the quantitative transfer law of its quality attributes. Methods Fifteen batches of WXY reference samples were prepared via water decoction. A UPLC fingerprint analysis method was established to identify key chromatographic peaks and determine the contents of chlorogenic acid, esculetin, cichoric acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, glehinnalin, and linarin were determined at 240 nm. Parameters such as transfer rate, extract yield, total statistical moment, and information entropy were calculated for both the herbs and the reference samples. Results The fingerprint similarity of the 15 batches of reference samples was greater than 0.9. Fourteen chromatographic peaks were identified, with 10 characteristic peaks defined. The extract yield ranged from 43.27% to 53.43%. The transfer rates of chlorogenic acid, esculetin, cichoric acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, glehinnalin, and linarin were 34.25%—42.72%, 51.52%—58.80%, 28.21%—32.04%, 56.26%—65.59%, 40.63%—53.89%, 22.35%—31.29%, 38.39%—46.22%, 34.02%—40.82%, 18.64%—20.96%, and 28.55%—32.63%, respectively, with no discrete data observed. The relative standard deviations (RSDs) of the information entropy for the UPLC fingerprints of the five herbs and the reference samples were all less than 10%. The RSDs of mean chromatographic retention time of total quantum (MCRTT ) and information entropy for WXY (crude herbs) were 3.90% and 2.25%, respectively. Conclusion Quality analysis of the reference samples based on the parameters of the supramolecular “imprinting template” provides a scientific basis for the quality control of WXY reference samples and the development of compound formulations.
classical famous formula
/
Wuwei Xiaodu Yin
/
benchmark substance
/
law of value transfer
/
supramolecular
/
imprinted template
/
quality attribute
/
fingerprint
/
chlorogenic acid
/
aesculetin
/
cichoric acid
/
neochlorogenic acid
/
cryptchlorogenic acid
/
isochlorogenic acid A
/
isochlorogenic acid B
/
isochlorogenic acid C
/
glifonlactone
/
limonide
/
total statistical moment
/
information entropy
肖强, 袁琴, 张子桉, 陈凡, 刘丹玲, 黄端阳, 曾慧, 潘雪, 贺福元, 肖美凤.
基于超分子“印迹模板”理论分析五味消毒饮基准样品的质量属性.
中草药,
2026
, 57
(11)
: 4147
-4157
.
DOI: 10.7501/j.issn.0253-2670.2026.11.007
XIAO Qiang, YUAN Qin, ZHANG Zian, CHEN Fan, LIU Danling, HUANG Duanyang, ZENG Hui, PAN Xue, HE Fuyuan, XIAO Meifeng.
Analysis on quality attribute of substance benchmarks of Wuwei Xiaodu Yin based on supramolecular “imprinting template” theory[J].
Chinese Traditional and Herbal Drugs ,
2026
, 57
(11)
: 4147
-4157
.
DOI: 10.7501/j.issn.0253-2670.2026.11.007
国家自然科学基金资助项目 (82274215); 国家自然科学基金资助项目 (82404815); 湖南省自然科学基金资助项目 (2025JJ80145); 湖南省中医药管理局 (B2024010); 湖南中医药大学研究生科研创新项目 (2025CX083); 湖南中医药大学研究生科研创新项目 (2025XC096); 湖南中医药大学研究生科研创新项目 (CX20251199)
参考文献
引证文献
国家中医药管理局, 国家药品监督管理局. 关于印发《古代经典名方目录（第一批）》的通知[EB/OL]. (2018-04-16) [2025-09-15]. http://www.natcm.gov.cn/kejisi/zhengcewenjian/2018-04-16/7107.html.
2026年第57卷第11期
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doi: 10.7501/j.issn.0253-2670.2026.11.007
接收时间：2025-11-25
首发时间：2026-09-09
https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.11.007
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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