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基于代谢组和转录组的酸枣仁活性成分生物合成关键基因的初步筛选研究

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中草药 | 药材与资源 2026,57(8): 3121-3141

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中草药 |药材与资源 2026 , 57 (8) : 3121 -3141

基于代谢组和转录组的酸枣仁活性成分生物合成关键基因的初步筛选研究

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陈继聪¹, 孙航¹, 练从龙¹, 马蕊¹, 赵乐¹, 陈随清¹, 刘秀玉^1,2

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通讯作者:

刘秀玉

作者简介:

陈继聪: 陈继聪,硕士研究生,研究方向为中药活性成分合成途径解析及其异源生产研究。Tel:19840988037 E-mail:chenjicong3210@163.com

Preliminary screening of key genes for biosynthesis of active components of Ziziphi Spinosae Semen based on metabolome and transcriptome

CHEN Jicong, SUN Hang, LIAN Conglong, MA Rui, ZHAO Le, CHEN Suiqing, LIU Xiuyu

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doi: 10.7501/j.issn.0253-2670.2026.08.024

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目的旨在深度挖掘参与斯皮诺素和酸枣仁皂苷A生物合成的关键基因。方法选取青果期和成熟期的酸枣仁Ziziphi Spinosae Semen作为供试材料，进行转录组和代谢组学分析，通过功能注释和差异表达基因分析，系统解析与斯皮诺素和酸枣仁皂苷A生物合成途径相关的关键候选基因。结果通过代谢组学分析共检测到1 646种代谢物；转录组测序及功能注释鉴定出7 316个差异表达基因。研究发现，斯皮诺素在酸枣仁青果期相对富集，酸枣仁皂苷A在成熟期显著富集。结合系统发育和热图分析，筛选出50个关键候选基因，包括33个UGTs、12个OMTs、2个OSCs和3个CYP450s基因，推测其可能参与斯皮诺素和酸枣仁皂苷A的下游生物合成途径。结论系统解析并推测了斯皮诺素和酸枣仁皂苷A的生物合成途径，为后续功能元件鉴定奠定基础，拓展了酸枣仁活性成分的分子生物学研究，为中药资源开发利用提供了新思路。

关键词

酸枣仁 / 代谢组学 / 转录组学 / 斯皮诺素 / 酸枣仁皂苷A

Abstract

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Objective This study aimed to comprehensively identify the key genes involved in the biosynthesis of spinosin and jujuboside A. Methods Ziziphi Spinosae Semen samples at green fruit and mature stages were selected as experimental materials to construct transcriptomic and metabolomic datasets. Key candidate genes associated with the spinosin and jujuboside A biosynthesis pathways were systematically analyzed through functional annotation and differential expression gene analysis. Results Metabolomic profiling detected 1 646 metabolites, while transcriptomic sequencing and functional annotation identified 7 316 differentially expressed genes. Results revealed that spinosin was relatively enriched during the green fruit stage of Ziziphi Spinosae Semen, whereas jujuboside A accumulated predominantly at the mature stage. Through phylogenetic and heatmap analyses, 50 key candidate genes were screened, including 33 UGTs, 12 OMTs, two OSCs, and three CYP450s, which are hypothesized to participate in the downstream biosynthesis of spinosin and jujuboside A. Conclusion This work systematically deciphers and proposes biosynthetic pathways for these components, laying a foundation for subsequent functional gene validation. It advances molecular biology research on Ziziphi Spinosae Semen bioactive compounds and provides novel insights for the development and utilization of traditional Chinese medicine resources.

Key words

Ziziphus jujube Mill. var. spinosa (Bunge) Hu ex H. F. Chou / metabolomics / transcriptomics / spinosin / jujuboside A

引用本文

陈继聪, 孙航, 练从龙, 马蕊, 赵乐, 陈随清, 刘秀玉. 基于代谢组和转录组的酸枣仁活性成分生物合成关键基因的初步筛选研究. 中草药, 2026 , 57 (8) : 3121 -3141 . DOI: 10.7501/j.issn.0253-2670.2026.08.024

CHEN Jicong, SUN Hang, LIAN Conglong, MA Rui, ZHAO Le, CHEN Suiqing, LIU Xiuyu. Preliminary screening of key genes for biosynthesis of active components of Ziziphi Spinosae Semen based on metabolome and transcriptome[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (8) : 3121 -3141 . DOI: 10.7501/j.issn.0253-2670.2026.08.024

基金

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河南中医药大学博士科研启动基金项目 (00104311-2024); 河南省高等学校青年骨干教师培养计划 (2021GGJS086); 中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目” (2060302)

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2026年第57卷第8期

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doi: 10.7501/j.issn.0253-2670.2026.08.024

接收时间：2025-12-02
首发时间：2026-09-09

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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