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AHP-CRITIC法结合BBD-RSM优选醋鳖甲炮制工艺及颜色-成分关联研究

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中草药 | 药剂与工艺 2026,57(8): 3019-3030

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中草药 |药剂与工艺 2026 , 57 (8) : 3019 -3030

AHP-CRITIC法结合BBD-RSM优选醋鳖甲炮制工艺及颜色-成分关联研究

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余荷蓉¹, 万欣¹, 闫瑞洋¹, 代树文², 张玖捌¹, 李昱¹, 毛靖¹, 梅茜¹, 赵晓莉¹, 毛春芹¹, 林丽娜², 陆兔林¹

作者信息

通讯作者:

林丽娜

作者简介:

余荷蓉: 余荷蓉,硕士研究生,研究方向为中药炮制及中药饮片质量标准。E-mail:935781685@qq.com

Optimization of processing technology of vinegar-quenched Pelodisci Carapax based on AHP-CRITIC combined with BBD-response surface technology and correlation between color and components

YU Herong, WAN Xin, YAN Ruiyang, DAI Shuwen, ZHANG Jiuba, LI Yu, MAO Jing, MEI Xi, ZHAO Xiaoli, MAO Chunqin, LIN Lina, LU Tulin

Affiliations

doi: 10.7501/j.issn.0253-2670.2026.08.015

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摘要

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目的优选醋鳖甲vinegar-quenched Pelodisci Carapax炮制工艺，探讨炮制过程中颜色与内在成分含量的关联性，为其工艺标准化与质量评价提供依据。方法采用Box-Behnken设计-响应面法（Box-Behnken design-response surface methodology，BBD-RSM），以砂烫温度、砂烫时间、醋淬后干燥时间为考察因素，以外观性状、水分、浸出物及6种氨基酸含量为考察指标，结合AHP-CRITIC法计算综合评分（overall desirability，OD）优化炮制工艺。采用色差仪测定炮制品的粉末颜色值（L*、a*、b*），分析其与成分含量的关联性。结果优选出醋鳖甲最佳工艺为砂烫温度211℃、砂烫时间7 min、醋淬后干燥时间121 min。相关性分析显示，L*值与脯氨酸含量呈极显著正相关（P＜0.01），与甘氨酸和缬氨酸含量呈显著正相关（P＜0.05）；b*值与羟脯氨酸、甘氨酸、精氨酸、脯氨酸、缬氨酸含量呈极显著负相关（P＜0.01）；a*值与甘氨酸含量呈极显著负相关（P＜0.01），与精氨酸、缬氨酸含量呈显著负相关（P＜0.05），E*值与脯氨酸呈显著正相关（P＜0.05）。回归性分析进一步确认颜色值与多成分含量之间存在量化关系。结论AHP-CRITIC法结合BBD-RSM法可科学优选醋鳖甲的炮制工艺，其颜色与成分显著相关，为建立醋鳖甲质量评价体系提供新方法。

关键词

醋鳖甲 / 炮制工艺 / AHP-CRITIC赋权法 / Box-Behnken设计-响应面法 / 色泽度 / 关联性分析

Abstract

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Objective To optimize the processing technology of Cubiejiɑ (vinegar-quenched Pelodisci Carapax, vqPC) and to investigate the correlation between color and intrinsic component content during processing, thereby providing a basis for process standardization and quality evaluation. Methods Using Box-Behnken design combined with response surface methodology (BBD-RSM), sand-frying temperature, sand-frying time, and drying time after vinegar quenching were selected as independent variables, while appearance characteristics, moisture content, extract contents, and the contents of six amino acids served as response variables. The analytic hierarchy process (AHP) and criteria importance through intercriteria correlation (CRITIC) method were integrated to calculate overall desirability (OD) for process optimization. Color values (L^*, a^*, b^*) of the processed powder were measured using a colorimeter and their correlations with component contents were analyzed. Results The optimal processing parameters were determined as follows: sand-frying temperature 211 ℃, sand-frying time 7 min, and drying time after vinegar quenching 121 min. Correlation analysis indicated that the L^* value showed a highly significant positive correlation with proline content (P < 0.01) and positive correlation with the contents of glycine and valine (P < 0.05). The b^* value exhibited a highly significant negative correlation with the contents of hydroxyproline, glycine, arginine, proline and valine (P < 0.01). The a^* value showed a highly significant negative correlation with glycine content (P < 0.01) and a significant negative correlation with arginine and valine content (P < 0.05). The E^* value showed a significant positive correlation with proline content (P < 0.05). Regression analysis further confirmed quantitative relationships between color values and the contents of multiple components. Conclusion The combination of AHP-CRITIC and BBD-RSM provides a scientific approach for optimizing the processing technology of vqPC. The significant correlations between color parameters and chemical components offer a new method for establishing its quality evaluation system.

Key words

vinegar-quenched Pelodisci Carapax / processing technology / AHP-CRITIC weighting method / Box-Behnken design-response surface methodology / chroma / correlation analysis

引用本文

余荷蓉, 万欣, 闫瑞洋, 代树文, 张玖捌, 李昱, 毛靖, 梅茜, 赵晓莉, 毛春芹, 林丽娜, 陆兔林. AHP-CRITIC法结合BBD-RSM优选醋鳖甲炮制工艺及颜色-成分关联研究. 中草药, 2026 , 57 (8) : 3019 -3030 . DOI: 10.7501/j.issn.0253-2670.2026.08.015

YU Herong, WAN Xin, YAN Ruiyang, DAI Shuwen, ZHANG Jiuba, LI Yu, MAO Jing, MEI Xi, ZHAO Xiaoli, MAO Chunqin, LIN Lina, LU Tulin. Optimization of processing technology of vinegar-quenched Pelodisci Carapax based on AHP-CRITIC combined with BBD-response surface technology and correlation between color and components[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (8) : 3019 -3030 . DOI: 10.7501/j.issn.0253-2670.2026.08.015

基金

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国家重点研发计划项目 (2023YFC3504200)

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排序方式：

佚名. 神农本草经 (中品·虫鱼部)[M]. 支晓娟, 整理. 广州:广东科技出版社, 2022:233.
中国药典[S]. 一部. 2025:414.
廖彭莹, 张天丰, 邓纭宁, 等. 鳖各部位的成分和药理作用研究进展[J]. 中药材, 2024, 47(4):1053-1061.
王红霞, 董玉秀. 不同产地鳖甲生片与醋鳖甲饮片紫外光谱鉴别[J]. 北京中医药大学学报, 2007, 30(8):543-546.
刘林增. 鳖甲炮制传统工艺沿革与现状[J]. 山东医学高等专科学校学报, 2012, 34(6):430-431.
黄清杰, 徐志伟. 砂炒法的历史沿革与研究进展[J]. 甘肃医药, 2020, 39(4):301-303.
Phang J M. The regulatory mechanisms of proline and hydroxyproline metabolism:Recent advances in perspective[J]. Front Oncol, 2023, 12:1118675.
董珂旭, 张媛, 高如汐, 等. AHP-熵权法结合Box-Behnken设计-响应面法优选枸杞汁制巴戟天炮制工艺[J]. 中草药, 2025, 56(7):2301-2311.
吴建华, 刘婧, 吴志瑰, 等. 颜色量化及其在中药中的应用研究进展[J]. 江西中医药大学学报, 2016, 28(5):114-115.
黄莉, 杨磊, 金凯, 等. AHP-CRITIC综合加权联用响应面法的蜜百合炮制工艺优选及颜色相关性分析[J]. 中草药, 2024, 55(18):6174-6185.
Li P, Wu G Y. Roles of dietary glycine, proline, and hydroxyproline in collagen synthesis and animal growth[J]. Amino Acids, 2018, 50(1):29-38.
杜冰冰, 郝莹莹, 吕龙贤. L-精氨酸对小鼠急性肝损伤的预防作用及肠道菌群的影响[J]. 中国微生态学杂志, 2025, 37(7):745-751.
Jian H F, Xu Q Q, Wang X M, et al. Amino acid and fatty acid metabolism disorders trigger oxidative stress and inflammatory response in excessive dietary valine-induced NAFLD of laying hens[J]. Front Nutr, 2022, 9:849767.
吕纯懿, 徐瑞荣. 升麻鳖甲汤研究进展[J]. 中华中医药学刊, 2025, 43(5):82-88.
李燕, 李笑, 刘妍君, 等. 青蒿鳖甲汤治疗肺部疾病的研究进展[J]. 中国实验方剂学杂志, 2024, 30(3):258-269.
浙江省食品药品监督管理局. 浙江省中药炮制规范-2015年版[M]. 北京:中国医药科技出版社, 2016:400.
江苏省药品监督管理局. 江苏省中药饮片炮制规范[M]. 南京:江苏科学技术出版社, 2002:456-457.
Wang S S, Chen G J, Chen K W, et al. Bioavailability and prebiotic potential of Carapax Trionycis, a waste from soft-shelled turtle processing[J]. J Sci Food Agric, 2020, 100(6):2554-2567.
邹惠亮, 陈褚建, 王新财, 等. UPLC-MS/MS法快速测定鳖甲胶中4种主要氨基酸的含量[J]. 中国药品标准, 2022, 23(5):535-540.
廖彭莹, 周利琴, 廖丹葵, 等. 柱前衍生化高效液相色谱法测定鳖甲生、制品中氨基酸的含量[J]. 中药材, 2016, 39(4):802-805.
刘绍姜, 钟凌云, 张弘弢, 等. 基于AHP-CRITIC法结合Box-Behnken设计-响应面法优选盐麸泽泻炮制工艺及药效研究[J]. 中草药, 2025, 56(8):2773-2785.
李东辉, 吴红伟, 李国峰, 等. 中药炮制前后化学成分转化的研究[J]. 沈阳药科大学学报, 2023, 40(1):106-114.
宫瑞泽, 霍晓慧, 张磊, 等. 美拉德反应对中药品质的影响及调控研究进展[J]. 中草药, 2019, 50(1):243-251.
王凯, 曲珍妮, 毕钰, 等. 中药炮制加工过程中美拉德反应的研究进展[J]. 中国实验方剂学杂志, 2025, 31(8):268-278.

2026年第57卷第8期

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doi: 10.7501/j.issn.0253-2670.2026.08.015

接收时间：2025-12-03
首发时间：2026-09-09

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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