Article(id=1304414874692509696, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414858296971266, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.06.009, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1761667200000, receivedDateStr=2025-10-29, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788926340982, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788926340982, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788926340982, creator=13701087609, updateTime=1788926340982, updator=13701087609, issue=Issue{id=1304414858296971266, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='6', pageStart='2009', pageEnd='2444', issueExtLink='null', onlineDate='null', pubDate='1774627200000', pubDateStr='2026-03-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788926337074, creator='13701087609', updateTime=1788926665348, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304416235240841997, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414858296971266, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304416235240841998, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414858296971266, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=2098, endPage=2108, ext={EN=ArticleExt(id=1304414875124523010, articleId=1304414874692509696, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Preparation, quality evaluation and pharmacodynamic study of naringenin micellar thermosensitive gel, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective To prepare naringenin micelles-loaded thermosensitive gel (NMTG), evaluate its biological safety and anti-inflammatory activity, and investigate its therapeutic effects on dry eye disease (DED) induced by benzalkonium chloride (BAC) in rats. Methods Naringenin micelles (NM) were prepared by the thin-film dispersion method. Quality evaluations of the prepared NM included appearance, particle size, ζ potential, transmission electron microscopy, encapsulation efficiency, drug loading and stability. Concentrations of Poloxamer 188 (P188) and Poloxamer 407 (P407) were optimized based on the phase transition temperatures (T gel and T atd gel ) of NMTG before and after dilution with tear fluid. Optimal concentrations of P188 and P407 were dissolved in NM to form NMTG. Biological safety, anti-inflammatory activity, and therapeutic effects on BAC-induced DED rats were investigated. Results Prepared NM was clear and transparent. Transmission electron microscopy showed near-spherical particles with an average particle size of (21.56 ± 0.16) nm, a polydispersity index (PDI) value of 0.053 ± 0.033, and a ζ potential of (-18.63 ± 1.94) mV. Encapsulation efficiency and drug loading were (73.96 ± 0.24)% and (7.34 ± 0.38)%, respectively, and the stability was good within 14 d. The optimal NMTG was achieved with concentrations of 5.29% P188 and 16.24% P407. NMTG demonstrated good biological safety and anti-inflammatory activity. Pharmacological experiments indicated that NMTG showed favorable therapeutic effects on DED rats. Conclusion The successful preparation of NMTG improve the solubility of naringenin, which endow it with good DED therapeutic effects. This will provide support for new indications of naringenin in ocular applications and offer candidate solutions for the development of new naringenin dosage forms., authors=LUO Qianfei, MA Ruoxin, SUN Wenjin, LIU Tingyu, ZHANG Haochen, JIN Mingyang, ZHOU Junfang, WANG Jianhui, LIU Shuling, CUI Teng, WANG Huiyun, authorsList=LUO Qianfei, MA Ruoxin, SUN Wenjin, LIU Tingyu, ZHANG Haochen, JIN Mingyang, ZHOU Junfang, WANG Jianhui, LIU Shuling, CUI Teng, WANG Huiyun, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304414875015471105, articleId=1304414874692509696, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=柚皮素胶束温敏凝胶的制备、质量评价及药效学研究, columnId=1304140189132149234, journalTitle=中草药, columnName=药剂与工艺, runingTitle=null, highlight=null, articleAbstract=目的 制备柚皮素胶束温敏凝胶（naringenin micelles-loaded thermosensitive gel，NMTG），评价其生物安全性和抗炎活性，并考察其对苯扎氯铵诱导干眼症（dry eye disease，DED）大鼠的治疗作用。方法 采用薄膜分散法制备柚皮素胶束（naringenin micelles，NM），并对其外观、粒径、ζ电位、透射电子显微镜（scanning electron microscopy，TEM）、包封率和载药量、稳定性等进行质量评价。以泪液稀释前、后NMTG相变温度T gel和T atd gel为考察指标，优选泊洛沙姆188（Poloxamer 188，P188）和泊洛沙姆407（Poloxamer 407，P407）的用量，将最优用量的P188和P407溶于NM中形成NMTG；研究其生物安全性、抗炎活性和对苯扎氯铵诱导DED大鼠的治疗作用。结果 制备的NM外观澄清透明，TEM观察NM呈类球形，平均粒径为（21.56±0.16）nm，多分散指数（polydispersity index，PDI）为0.053±0.033，ζ电位为（-18.63±1.94）mV，包封率为（73.96±0.24）%，载药量为（7.34±0.38）%，在14 d内稳定性良好。以P188、P407用量分别为5.29%和16.24%制得最佳NMTG；NMTG具有良好的生物安全性和抗炎活性，并且药效学实验证明NMTG对DED大鼠具有良好的治疗作用。结论 成功制备NMTG，提高柚皮素溶解度以使其具有良好的DED治疗作用，将为柚皮素在眼部应用的新适应证提供支持，并为柚皮素新剂型的开发提供候选方案。, authors=罗倩菲1,2 , 马若馨2 , 孙文进2 , 刘庭宇2 , 张浩琛2 , 靳茗扬2 , 周俊芳2 , 王建辉2 , 刘树玲2 , 崔腾2 , 王慧云2 , authorsList=罗倩菲, 马若馨, 孙文进, 刘庭宇, 张浩琛, 靳茗扬, 周俊芳, 王建辉, 刘树玲, 崔腾, 王慧云, authorCompany=1 山东第二医科大学药学院, 山东 潍坊 261053; et al . Topical application of Liriope platyphylla extract attenuates dry eye syndrome induced by particulate matter[J]. J Ophthalmol , 2019, 2019: 1429548.et al . Protective effect of the natural flavonoid naringenin in mouse models of retinal injury[J]. Eur J Pharmacol , 2024, 962: 176231., et al. Evaluation of the effect of naringenin liposomal formulation on retinopathy in an experimental rabbit model[J]. Kafkas Univ Vet Fak , 2022, 28(4): 469-479.et al . Recent developments of nanostructures for the ocular delivery of natural compounds[J]. Front Chem , 2022, 10: 850757.et al . Nanomicelle-assisted targeted ocular delivery with enhanced antiinflammatory efficacy in vivo [J]. Adv Sci , 2017, 5(1): 1700455.
中草药
|药剂与工艺
2026
, 57
(6) :
2098
-2108
柚皮素胶束温敏凝胶的制备、质量评价及药效学研究
全屏
罗倩菲1,2 , 马若馨2 , 孙文进2 , 刘庭宇2 , 张浩琛2 , 靳茗扬2 , 周俊芳2 , 王建辉2 , 刘树玲2 , 崔腾2 , 王慧云2
作者信息
1 山东第二医科大学药学院, 山东 潍坊 261053;
作者简介:
罗倩菲: 罗倩菲(2000-),女,硕士研究生,研究方向为纳米药物递送系统。E-mail:luoqianfei@126.com
Preparation, quality evaluation and pharmacodynamic study of naringenin micellar thermosensitive gel
LUO Qianfei, MA Ruoxin, SUN Wenjin, LIU Tingyu, ZHANG Haochen, JIN Mingyang, ZHOU Junfang, WANG Jianhui, LIU Shuling, CUI Teng, WANG Huiyun
Affiliations
doi: 10.7501/j.issn.0253-2670.2026.06.009
文章导航
目的 制备柚皮素胶束温敏凝胶（naringenin micelles-loaded thermosensitive gel，NMTG），评价其生物安全性和抗炎活性，并考察其对苯扎氯铵诱导干眼症（dry eye disease，DED）大鼠的治疗作用。方法 采用薄膜分散法制备柚皮素胶束（naringenin micelles，NM），并对其外观、粒径、ζ电位、透射电子显微镜（scanning electron microscopy，TEM）、包封率和载药量、稳定性等进行质量评价。以泪液稀释前、后NMTG相变温度T gel和T atd gel为考察指标，优选泊洛沙姆188（Poloxamer 188，P188）和泊洛沙姆407（Poloxamer 407，P407）的用量，将最优用量的P188和P407溶于NM中形成NMTG；研究其生物安全性、抗炎活性和对苯扎氯铵诱导DED大鼠的治疗作用。结果 制备的NM外观澄清透明，TEM观察NM呈类球形，平均粒径为（21.56±0.16）nm，多分散指数（polydispersity index，PDI）为0.053±0.033，ζ电位为（-18.63±1.94）mV，包封率为（73.96±0.24）%，载药量为（7.34±0.38）%，在14 d内稳定性良好。以P188、P407用量分别为5.29%和16.24%制得最佳NMTG；NMTG具有良好的生物安全性和抗炎活性，并且药效学实验证明NMTG对DED大鼠具有良好的治疗作用。结论 成功制备NMTG，提高柚皮素溶解度以使其具有良好的DED治疗作用，将为柚皮素在眼部应用的新适应证提供支持，并为柚皮素新剂型的开发提供候选方案。
柚皮素
/
胶束
/
温敏凝胶
/
干眼症
/
薄膜分散法
/
星点设计-效应面法
/
药效学
/
生物安全性
/
抗炎活性
Objective To prepare naringenin micelles-loaded thermosensitive gel (NMTG), evaluate its biological safety and anti-inflammatory activity, and investigate its therapeutic effects on dry eye disease (DED) induced by benzalkonium chloride (BAC) in rats. Methods Naringenin micelles (NM) were prepared by the thin-film dispersion method. Quality evaluations of the prepared NM included appearance, particle size, ζ potential, transmission electron microscopy, encapsulation efficiency, drug loading and stability. Concentrations of Poloxamer 188 (P188) and Poloxamer 407 (P407) were optimized based on the phase transition temperatures (T gel and T atd gel ) of NMTG before and after dilution with tear fluid. Optimal concentrations of P188 and P407 were dissolved in NM to form NMTG. Biological safety, anti-inflammatory activity, and therapeutic effects on BAC-induced DED rats were investigated. Results Prepared NM was clear and transparent. Transmission electron microscopy showed near-spherical particles with an average particle size of (21.56 ± 0.16) nm, a polydispersity index (PDI) value of 0.053 ± 0.033, and a ζ potential of (-18.63 ± 1.94) mV. Encapsulation efficiency and drug loading were (73.96 ± 0.24)% and (7.34 ± 0.38)%, respectively, and the stability was good within 14 d. The optimal NMTG was achieved with concentrations of 5.29% P188 and 16.24% P407. NMTG demonstrated good biological safety and anti-inflammatory activity. Pharmacological experiments indicated that NMTG showed favorable therapeutic effects on DED rats. Conclusion The successful preparation of NMTG improve the solubility of naringenin, which endow it with good DED therapeutic effects. This will provide support for new indications of naringenin in ocular applications and offer candidate solutions for the development of new naringenin dosage forms.
naringenin
/
micelles
/
thermosensitive gel
/
dry eye disease
/
thin-film dispersion method
/
star-point design-response surface method
/
pharmacodynamics
/
biological safety
/
anti-inflammatory activity
罗倩菲, 马若馨, 孙文进, 刘庭宇, 张浩琛, 靳茗扬, 周俊芳, 王建辉, 刘树玲, 崔腾, 王慧云.
柚皮素胶束温敏凝胶的制备、质量评价及药效学研究.
中草药,
2026
, 57
(6)
: 2098
-2108
.
DOI: 10.7501/j.issn.0253-2670.2026.06.009
LUO Qianfei, MA Ruoxin, SUN Wenjin, LIU Tingyu, ZHANG Haochen, JIN Mingyang, ZHOU Junfang, WANG Jianhui, LIU Shuling, CUI Teng, WANG Huiyun.
Preparation, quality evaluation and pharmacodynamic study of naringenin micellar thermosensitive gel[J].
Chinese Traditional and Herbal Drugs ,
2026
, 57
(6)
: 2098
-2108
.
DOI: 10.7501/j.issn.0253-2670.2026.06.009
参考文献
引证文献
邢悦, 张晓梅. 干眼病因的研究进展[J]. 中国现代医学杂志, 2021, 31(18): 49-54.et al . Topical application of Liriope platyphylla extract attenuates dry eye syndrome induced by particulate matter[J]. J Ophthalmol , 2019, 2019: 1429548.et al . Protective effect of the natural flavonoid naringenin in mouse models of retinal injury[J]. Eur J Pharmacol , 2024, 962: 176231., et al. Evaluation of the effect of naringenin liposomal formulation on retinopathy in an experimental rabbit model[J]. Kafkas Univ Vet Fak , 2022, 28(4): 469-479.et al . Recent developments of nanostructures for the ocular delivery of natural compounds[J]. Front Chem , 2022, 10: 850757.et al . Nanomicelle-assisted targeted ocular delivery with enhanced antiinflammatory efficacy in vivo [J]. Adv Sci , 2017, 5(1): 1700455.
2026年第57卷第6期
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doi: 10.7501/j.issn.0253-2670.2026.06.009
接收时间：2025-10-29
首发时间：2026-09-09
https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.06.009
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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