Article(id=1304414804182065853, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414798482010221, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.05.006, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1760630400000, receivedDateStr=2025-10-17, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788926324172, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788926324172, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788926324172, creator=13701087609, updateTime=1788926324172, updator=13701087609, issue=Issue{id=1304414798482010221, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='5', pageStart='1597', pageEnd='2008', issueExtLink='null', onlineDate='null', pubDate='1773244800000', pubDateStr='2026-03-12', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788926322813, creator='13701087609', updateTime=1788926625459, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304416067925864795, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414798482010221, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304416067925864796, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304414798482010221, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1647, endPage=1657, ext={EN=ArticleExt(id=1304414804920263359, articleId=1304414804182065853, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Preparation and characterization of photo-controlled release and reactive oxygen species-responsive elemene/chlorin e6 co-loaded liposomes and their anti-bladder cancer 5637 cell activity in vitro , columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective Reactive oxygen species (ROS)-responsive co-loaded elemene (Ee) and chlorin e6 (Ce6) liposomes (Ee-Ce6-L) were prepared, characterization of their physicochemical properties, and evaluated for their inhibitory effects on bladder cancer 5637 cells. Methods The synergistic ratio of elemene and Ce6 (Ee + Ce6) was screened using the MTT assay. Ee-Ce6-L was prepared via the thin-film hydration method and characterized for morphology, particle size, encapsulation efficiency, drug loading capacity, stability, ROS generation capacity, and in vitro release rate. MTT assays evaluated the cytotoxicity of Ee-Ce6-L against 5637 cells. Annexin V-FITC/PI double staining was used to study the apoptotic effects of Ee-Ce6-L on cells. Fluorescence microscopy and a fluorometer were employed to examine its impact on ROS generation capacity and mitochondrial membrane potential (MMP). Western blotting analysis was conducted to investigate the expression of apoptosis pathway-related proteins. Results Ee + Ce6 exhibited synergistic effects across the 40:1 to 200:1 concentration range, with optimal synergy observed at 120:1; Ee-Ce6-L exhibited good stability and dispersibility. After irradiation with 660 nm laser, the particle size changed from (194.27 ± 0.20) nm to (390.97 ± 2.76) nm, and the ζ potential shifted from (−19.06 ± 0.07) mV to (−6.30 ± 0.34) mV. In vitro release experiments demonstrated that Ee-Ce6-L exhibits dual functions of sustained release and light-controlled drug release; the encapsulation efficiencies of elemene and Ce6 were (94.02 ± 0.38)% and (96.95 ± 0.70)%, respectively, while the drug loading capacities of Ee and Ce6 were (11.51 ± 0.46)% and (2.32 ± 0.01)%; Ee-Ce6-L exhibited strong singlet oxygen generation capacity; In vitro cell experiments demonstrated that Ee-Ce6-L promoted apoptosis in 5637 cells by increasing ROS production and reducing MMP, as well as by decreasing B-cell lymphoma-2 (Bcl-2) protein expression while increasing Bcl-2-associated X protein (Bax), cleaved poly ADP-ribose polymerase (cleaved PARP), and cleaved cystein-asparate protease-3 (cleaved Caspase-3) protein expression. Conclusion Ee-Ce6-L exhibits ROS-responsive release properties and synergistically inhibits 5637 cell proliferation and induces apoptosis through the combined effects of traditional Chinese medicine and phototherapy., authors=WANG Aijia, TIAN Huan, GUO Dongyan, ZHAO Feng, YUE Baosen, CHENG Jiangxue, ZOU Junbo, SHI Yajun, ZHANG Xiaofei, ZHAI Bingtao, authorsList=WANG Aijia, TIAN Huan, GUO Dongyan, ZHAO Feng, YUE Baosen, CHENG Jiangxue, ZOU Junbo, SHI Yajun, ZHANG Xiaofei, ZHAI Bingtao, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304414804848960190, articleId=1304414804182065853, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=光控释活性氧响应的共载榄香烯和二氢卟吩e6脂质体的制备、表征及体外抗膀胱癌5637细胞活性研究, columnId=1304140189132149234, journalTitle=中草药, columnName=药剂与工艺, runingTitle=null, highlight=null, articleAbstract=目的 制备活性氧（reactive oxygen species，ROS）响应的共载榄香烯（elemene，Ee）和二氢卟吩e6（chlorin e6，Ce6）脂质体（Ee-Ce6-L），对其理化性质进行表征，并研究Ee-Ce6-L对膀胱癌5637细胞的抑制作用。方法 采用MTT法筛选榄香烯与Ce6（Ee+Ce6）的协同比例，通过薄膜水化法制备Ee-Ce6-L，并对其形态、粒径、包封率和载药量、稳定性、ROS生成能力、体外释放度等性能进行表征；MTT实验考察Ee-Ce6-L对5637细胞的毒性；Annexin V-FITC/PI双染实验研究Ee-Ce6-L对细胞的凋亡作用；荧光显微镜及荧光酶标仪考察其对ROS生成能力及线粒体膜电位（mitochondrial membrane potential，MMP）的影响；通过免疫印迹法（Western blotting）考察凋亡通路相关蛋白的表达。结果 Ee+Ce6在40∶1～200∶1均具有协同作用，且在120∶1时协同效果最好；Ee-Ce6-L具有较好的稳定性和分散性，经660 nm激光照射后粒径由（194.27±0.20）nm变为（390.97±2.76）nm，ζ电位由（-19.06±0.07）mV变为（-6.30±0.34）mV；体外释放实验表明，Ee-Ce6-L具有缓释和光控释药的双重作用；榄香烯和Ce6的包封率分别为（94.02±0.38）%、（96.95±0.70）%，榄香烯和Ce6的载药量分别为（11.51±0.46）%、（2.32±0.01）%；Ee-Ce6-L有较强的单线态氧生成能力；体外细胞实验表明，Ee-Ce6-L能够通过增加ROS的产生和降低MMP，以及降低B细胞淋巴瘤-2（B-cell lymphoma-2，Bcl-2）蛋白表达，增加Bcl-2相关X蛋白（Bcl-2-associated X protein，Bax），裂解的多聚ADP核糖聚合酶（cleaved poly ADP-ribose polymerase，cleaved PARP）和裂解的半胱氨酸天冬氨酸蛋白酶-3（cleaved cystein-asparate protease-3，cleaved Caspase-3）蛋白的表达，促进5637细胞凋亡。结论 Ee-Ce6-L具有ROS响应释放特性，其能够通过中药-光疗作用协同抑制5637细胞增殖和诱导细胞凋亡。, authors=王艾佳1 , 田欢2 , 郭东艳1,3 , 赵锋2,4 , 岳宝森2,5 , 程江雪1 , 邹俊波1 , 史亚军1 , 张小飞1,6 , 翟秉涛1,6 , authorsList=王艾佳, 田欢, 郭东艳, 赵锋, 岳宝森, 程江雪, 邹俊波, 史亚军, 张小飞, 翟秉涛, authorCompany=1 陕西中医药大学药学院, 陕西 咸阳 712046; et al . Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin , 2024, 74(3): 229-263.et al . A photosensitizer-loaded DNA origami nanosystem for photodynamic therapy[J]. ACS Nano , 2016, 10(3): 3486-3495.et al . An injectable dipeptide-fullerene supramolecular hydrogel for photodynamic antibacterial therapy[J]. J Mater Chem B , 2018, 6(44): 7335-7342.et al . Chlorin e6 loaded lactoferrin nanoparticles for enhanced photodynamic therapy[J]. J Mater Chem B , 2017, 5(46): 9189-9196.et al . Ce6 nanoassemblies: Molecular mechanism and strategies for combinational anticancer therapy[J]. Aggregate , 2024, 5(1): e443.et al . Emerging combination strategies with phototherapy in cancer nanomedicine[J]. Chem Soc Rev , 2020, 49(22): 8065-8087.et al . Recent advances in understanding the mechanisms of elemene in reversing drug resistance in tumor cells: A review[J]. Molecules , 2021, 26(19): 5792.et al . β-elemene enhances cisplatin-induced apoptosis in bladder cancer cells through the ROS-AMPK signaling pathway[J]. Oncol Lett , 2020, 19(1): 291-300.et al . Liposome co-loaded with β-elemene and IR780 for combined chemo-phototherapy[J]. J Drug Deliv Sci Technol , 2022, 68: 103122.Saudi J Biol Sci , 2022, 29(6): 103298.Cancer Res , 2010, 70(2): 440-446.et al . Preparation of photo-controlled release ROS-responsive Ce6/elemene co-loaded liposomes and study on the effect on enhancing apoptosis of NMIBC[J]. Biomed Pharmacother , 2024, 179: 117398.J Vis Exp , 2020(160): e60682.et al . Light-responsive Ru-Se coordinated nanoreactor spatiotemporally relieves hypoxia for photodynamic therapy of bladder cancer[J]. ACS Appl Mater Interfaces , 2025, 17(20): 29392-29406.et al . Molecular targets of β-elemene, a herbal extract used in traditional Chinese medicine, and its potential role in cancer therapy: A review[J]. Biomed Pharmacother , 2019, 114: 108812.et al . Perspectives on cutting-edge nanoparticulate drug delivery technologies based on lipids and their applications[J]. Chem Biol Drug Des , 2023, 102(2): 377-394.Nano Lett , 2021, 21(1): 3-5.Wiley Interdiscip Rev Nanomed Nanobiotechnol , 2017, 9(5). DOI:10.1002/wnan.1450.et al . Repeatable and adjustable on-demand sciatic nerve block with phototriggerable liposomes[J]. Proc Natl Acad Sci USA , 2015, 112(51): 15719-15724.Int J Pharm , 2021, 593: 120148.et al . Enhancement of tumor lethality of ROS in photodynamic therapy[J]. Cancer Med , 2021, 10(1): 257-268.)
中草药
|药剂与工艺
2026
, 57
(5) :
1647
-1657
光控释活性氧响应的共载榄香烯和二氢卟吩e6脂质体的制备、表征及体外抗膀胱癌5637细胞活性研究
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王艾佳, 田欢, 郭东艳, 赵锋, 岳宝森, 程江雪, 邹俊波, 史亚军, 张小飞, 翟秉涛
作者信息
通讯作者:
翟秉涛
作者简介:
王艾佳: 王艾佳(2003—),女,硕士研究生,从事中药新制剂与新剂型研究。E-mail:15091611352@163.com
Preparation and characterization of photo-controlled release and reactive oxygen species-responsive elemene/chlorin e6 co-loaded liposomes and their anti-bladder cancer 5637 cell activity in vitro
WANG Aijia, TIAN Huan, GUO Dongyan, ZHAO Feng, YUE Baosen, CHENG Jiangxue, ZOU Junbo, SHI Yajun, ZHANG Xiaofei, ZHAI Bingtao
Affiliations
doi: 10.7501/j.issn.0253-2670.2026.05.006
文章导航
目的 制备活性氧（reactive oxygen species，ROS）响应的共载榄香烯（elemene，Ee）和二氢卟吩e6（chlorin e6，Ce6）脂质体（Ee-Ce6-L），对其理化性质进行表征，并研究Ee-Ce6-L对膀胱癌5637细胞的抑制作用。方法 采用MTT法筛选榄香烯与Ce6（Ee+Ce6）的协同比例，通过薄膜水化法制备Ee-Ce6-L，并对其形态、粒径、包封率和载药量、稳定性、ROS生成能力、体外释放度等性能进行表征；MTT实验考察Ee-Ce6-L对5637细胞的毒性；Annexin V-FITC/PI双染实验研究Ee-Ce6-L对细胞的凋亡作用；荧光显微镜及荧光酶标仪考察其对ROS生成能力及线粒体膜电位（mitochondrial membrane potential，MMP）的影响；通过免疫印迹法（Western blotting）考察凋亡通路相关蛋白的表达。结果 Ee+Ce6在40∶1～200∶1均具有协同作用，且在120∶1时协同效果最好；Ee-Ce6-L具有较好的稳定性和分散性，经660 nm激光照射后粒径由（194.27±0.20）nm变为（390.97±2.76）nm，ζ电位由（-19.06±0.07）mV变为（-6.30±0.34）mV；体外释放实验表明，Ee-Ce6-L具有缓释和光控释药的双重作用；榄香烯和Ce6的包封率分别为（94.02±0.38）%、（96.95±0.70）%，榄香烯和Ce6的载药量分别为（11.51±0.46）%、（2.32±0.01）%；Ee-Ce6-L有较强的单线态氧生成能力；体外细胞实验表明，Ee-Ce6-L能够通过增加ROS的产生和降低MMP，以及降低B细胞淋巴瘤-2（B-cell lymphoma-2，Bcl-2）蛋白表达，增加Bcl-2相关X蛋白（Bcl-2-associated X protein，Bax），裂解的多聚ADP核糖聚合酶（cleaved poly ADP-ribose polymerase，cleaved PARP）和裂解的半胱氨酸天冬氨酸蛋白酶-3（cleaved cystein-asparate protease-3，cleaved Caspase-3）蛋白的表达，促进5637细胞凋亡。结论 Ee-Ce6-L具有ROS响应释放特性，其能够通过中药-光疗作用协同抑制5637细胞增殖和诱导细胞凋亡。
榄香烯
/
二氢卟吩e6
/
脂质体
/
膀胱癌
/
光动力疗法
Objective Reactive oxygen species (ROS)-responsive co-loaded elemene (Ee) and chlorin e6 (Ce6) liposomes (Ee-Ce6-L) were prepared, characterization of their physicochemical properties, and evaluated for their inhibitory effects on bladder cancer 5637 cells. Methods The synergistic ratio of elemene and Ce6 (Ee + Ce6) was screened using the MTT assay. Ee-Ce6-L was prepared via the thin-film hydration method and characterized for morphology, particle size, encapsulation efficiency, drug loading capacity, stability, ROS generation capacity, and in vitro release rate. MTT assays evaluated the cytotoxicity of Ee-Ce6-L against 5637 cells. Annexin V-FITC/PI double staining was used to study the apoptotic effects of Ee-Ce6-L on cells. Fluorescence microscopy and a fluorometer were employed to examine its impact on ROS generation capacity and mitochondrial membrane potential (MMP). Western blotting analysis was conducted to investigate the expression of apoptosis pathway-related proteins. Results Ee + Ce6 exhibited synergistic effects across the 40:1 to 200:1 concentration range, with optimal synergy observed at 120:1; Ee-Ce6-L exhibited good stability and dispersibility. After irradiation with 660 nm laser, the particle size changed from (194.27 ± 0.20) nm to (390.97 ± 2.76) nm, and the ζ potential shifted from (−19.06 ± 0.07) mV to (−6.30 ± 0.34) mV. In vitro release experiments demonstrated that Ee-Ce6-L exhibits dual functions of sustained release and light-controlled drug release; the encapsulation efficiencies of elemene and Ce6 were (94.02 ± 0.38)% and (96.95 ± 0.70)%, respectively, while the drug loading capacities of Ee and Ce6 were (11.51 ± 0.46)% and (2.32 ± 0.01)%; Ee-Ce6-L exhibited strong singlet oxygen generation capacity; In vitro cell experiments demonstrated that Ee-Ce6-L promoted apoptosis in 5637 cells by increasing ROS production and reducing MMP, as well as by decreasing B-cell lymphoma-2 (Bcl-2) protein expression while increasing Bcl-2-associated X protein (Bax), cleaved poly ADP-ribose polymerase (cleaved PARP), and cleaved cystein-asparate protease-3 (cleaved Caspase-3) protein expression. Conclusion Ee-Ce6-L exhibits ROS-responsive release properties and synergistically inhibits 5637 cell proliferation and induces apoptosis through the combined effects of traditional Chinese medicine and phototherapy.
elemene
/
chlorin e6
/
liposome
/
bladder cancer
/
photodynamic therapy
王艾佳, 田欢, 郭东艳, 赵锋, 岳宝森, 程江雪, 邹俊波, 史亚军, 张小飞, 翟秉涛.
光控释活性氧响应的共载榄香烯和二氢卟吩e6脂质体的制备、表征及体外抗膀胱癌5637细胞活性研究.
中草药,
2026
, 57
(5)
: 1647
-1657
.
DOI: 10.7501/j.issn.0253-2670.2026.05.006
WANG Aijia, TIAN Huan, GUO Dongyan, ZHAO Feng, YUE Baosen, CHENG Jiangxue, ZOU Junbo, SHI Yajun, ZHANG Xiaofei, ZHAI Bingtao.
Preparation and characterization of photo-controlled release and reactive oxygen species-responsive elemene/chlorin e6 co-loaded liposomes and their anti-bladder cancer 5637 cell activity in vitro [J].
Chinese Traditional and Herbal Drugs ,
2026
, 57
(5)
: 1647
-1657
.
DOI: 10.7501/j.issn.0253-2670.2026.05.006
国家自然科学基金青年项目 (82505363); 国家自然科学基金青年项目 (82204935); 国家中医药管理局赵锋全国老药工传承工作室建设项目 (国中医药人教函［2024］255号); 国家中医药管理局岳宝森全国老药工传承工作室建设项目 (国中医药人教函［2025］181号); 陕西省教育厅重点科学研究计划项目 (25JR058); 陕西中医药大学学科创新团队项目 (2019-YL11); 西安市卫生健康委员会项目 (2025qn01)
参考文献
引证文献
Bray F, Laversanne M, Sung H, et al . Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin , 2024, 74(3): 229-263.et al . A photosensitizer-loaded DNA origami nanosystem for photodynamic therapy[J]. ACS Nano , 2016, 10(3): 3486-3495.et al . An injectable dipeptide-fullerene supramolecular hydrogel for photodynamic antibacterial therapy[J]. J Mater Chem B , 2018, 6(44): 7335-7342.et al . Chlorin e6 loaded lactoferrin nanoparticles for enhanced photodynamic therapy[J]. J Mater Chem B , 2017, 5(46): 9189-9196.et al . Ce6 nanoassemblies: Molecular mechanism and strategies for combinational anticancer therapy[J]. Aggregate , 2024, 5(1): e443.et al . Emerging combination strategies with phototherapy in cancer nanomedicine[J]. Chem Soc Rev , 2020, 49(22): 8065-8087.et al . Recent advances in understanding the mechanisms of elemene in reversing drug resistance in tumor cells: A review[J]. Molecules , 2021, 26(19): 5792.et al . β-elemene enhances cisplatin-induced apoptosis in bladder cancer cells through the ROS-AMPK signaling pathway[J]. Oncol Lett , 2020, 19(1): 291-300.et al . Liposome co-loaded with β-elemene and IR780 for combined chemo-phototherapy[J]. J Drug Deliv Sci Technol , 2022, 68: 103122.Saudi J Biol Sci , 2022, 29(6): 103298.Cancer Res , 2010, 70(2): 440-446.et al . Preparation of photo-controlled release ROS-responsive Ce6/elemene co-loaded liposomes and study on the effect on enhancing apoptosis of NMIBC[J]. Biomed Pharmacother , 2024, 179: 117398.J Vis Exp , 2020(160): e60682.et al . Light-responsive Ru-Se coordinated nanoreactor spatiotemporally relieves hypoxia for photodynamic therapy of bladder cancer[J]. ACS Appl Mater Interfaces , 2025, 17(20): 29392-29406.et al . Molecular targets of β-elemene, a herbal extract used in traditional Chinese medicine, and its potential role in cancer therapy: A review[J]. Biomed Pharmacother , 2019, 114: 108812.et al . Perspectives on cutting-edge nanoparticulate drug delivery technologies based on lipids and their applications[J]. Chem Biol Drug Des , 2023, 102(2): 377-394.Nano Lett , 2021, 21(1): 3-5.Wiley Interdiscip Rev Nanomed Nanobiotechnol , 2017, 9(5). DOI:10.1002/wnan.1450.et al . Repeatable and adjustable on-demand sciatic nerve block with phototriggerable liposomes[J]. Proc Natl Acad Sci USA , 2015, 112(51): 15719-15724.Int J Pharm , 2021, 593: 120148.et al . Enhancement of tumor lethality of ROS in photodynamic therapy[J]. Cancer Med , 2021, 10(1): 257-268.
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doi: 10.7501/j.issn.0253-2670.2026.05.006
接收时间：2025-10-17
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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